Polyethyleneimine-Mediated Functionalization of Multiwalled Carbon Nanotubes: Synthesis, Characterization, and In Vitro Toxicity Assay

We have investigated the covalent conjugation of aminated multiwalled carbon nanotubes (MWCNTNH2)s with Oxidized Regenerated Cellulose (ORC) in order to enhance the hemostatic effect. The MWCNT-NH2s were prepared by functionalization of pristine MWCNTs (pMWCNTs) using amine groups. Neat ORC gauze and MWCNT-NH2s were reacted using glutamic acid as cross linking bridge. We investigated an amination of pMWCNTs as well as the dispersion of MWCNT-NH2s in the ORC gauze as matrix and their interfacial interactions by SEM and FT-IR. The results revealed that relatively strong interaction exists between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results in the significant increment of water uptake of MWCNT-NH2s/ORC composites with increasing the concentration of MWCNT-NH2s in composite. The in-vitro procoagulation test shows that the MWCNT-NH2s/ORC gauzes have significant procoagulant activity. The hemostatic evaluation of MWCNT-NH2s/ORC composites on rabbits shows that the aminated MWCNTs increase the rate of blood stopping and hence they decrease the blood loosing from injured sites. Hemostatic evaluation indicates that the MWCNT-NH2s/ORC gauze has a valuable hemostatic performance. The products of platelets release reaction, activated platelets glycoprotein and activated clotting enzymes were increased simultaneously. The mechanism of the hemostasis for MWCNT-NH2s/ORC gauze is discussed.

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Enhanced laser thermal ablation for the in vitro treatment of liver cancer by specific delivery of multiwalled carbon nanotubes functionalized with human serum albumin

International Journal of Nanomedicine ◽

10.2147/ijn.s15841 ◽

2011 ◽

pp. 129 ◽

Cited By ~ 47

Author(s):

Cornel Iancu ◽

Constantin ◽

Catoi Cornel ◽

Flaviu ◽

Rares ◽

...

Keyword(s):

Carbon Nanotubes ◽

Human Serum Albumin ◽

Liver Cancer ◽

Serum Albumin ◽

Human Serum ◽

Multiwalled Carbon Nanotubes ◽

Thermal Ablation ◽

Multiwalled Carbon ◽

In Vitro Treatment

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Nitrogen-Doped Multiwalled Carbon Nanotubes Inhibit Fat Deposition via Immunomodulatory Actions

10.21203/rs.3.rs-960318/v1 ◽

2021 ◽

Author(s):

Haifang Li ◽

Xue Xiao ◽

Geng Hu ◽

Dalin He ◽

Wenqian Zhang ◽

...

Keyword(s):

Carbon Nanotubes ◽

Multiwalled Carbon Nanotubes ◽

Mammalian Cells ◽

Fat Metabolism ◽

Fat Deposition ◽

The Body ◽

Inflammatory Factors ◽

Multiwalled Carbon ◽

Nitrogen Doped

Abstract Multiwalled carbon nanotubes (MWCNTs) offer immense opportunities to deliver drug and biomolecules to targeted tissues. However, it’s unclear for us about their effects on fat metabolism. Here, we demonstrate that nitrogen-doped carboxylate-functionalized MWCNTs (N-MWCNTs) inhibit fat deposition both in vivo and in vitro primarily by suppressing adipogenesis. N-MWCNTs show good biocompatability in HEK293 mammalian cells. Intramuscular administration of N-MWCNTs does not affect the body weight gain and feed intake of mice, but reduces the fat mass. In in vitro-cultured adipocytes, N-MWCNTs suppress fat accumulation, accompanying with decreased and increased expression of adipogenic and lipolysis genes, respectively. Transcriptome analysis further certified the N-MWCNT alteration of fat metabolism-related genes. Interestingly, we observed the phagocytosis of N-MWCNTs by macrophage-like cells via TEM imaging. The mRNA sequencing data also showed remarkable variation of the genes involved in TLRs pathway, ultimately leading to down- or up-regulation of inflammatory factors, of which Tnfα, Il1, Il7, Il10, and Il12 are decreased, whereas Il6 and Il11 are increased. In conclusion, N-MWCNTs induce the production of inflammatory cytokines through immune responses, which trigger the reduction of fat deposition. These findings support the usage of N-MWCNTs as a promising delivery for anti-obesity agents.

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In vitro cytotoxicity of calcium phosphate cement reinforced with multiwalled carbon nanotubes

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-019-6256-4 ◽

2019 ◽

Vol 30 (5) ◽

Cited By ~ 1

Author(s):

Lubomir Medvecky ◽

Maria Giretova ◽

Ruzena Kralikova ◽

Simona Medvecka ◽

Jaroslav Briancin

Keyword(s):

Carbon Nanotubes ◽

Calcium Phosphate ◽

Multiwalled Carbon Nanotubes ◽

Calcium Phosphate Cement ◽

In Vitro Cytotoxicity ◽

Multiwalled Carbon

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Preparation, Characterization, andIn VitroandVivoAntitumor Activity of Oridonin-Conjugated Multiwalled Carbon Nanotubes Functionalized with Carboxylic Group

Journal of Nanomaterials ◽

10.1155/2016/3439419 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Chuanjin Wang ◽

Wei Li

Keyword(s):

Carbon Nanotubes ◽

Multiwalled Carbon Nanotubes ◽

Tumor Therapy ◽

Water Soluble ◽

Carboxylic Group ◽

Multiwalled Carbon ◽

Compound C ◽

Poorly Water Soluble

Carbon nanotubes have shown great potential in tumor therapy. Oridonin (ORI) is a poorly water-soluble diterpenoid compound (C20H28O6) used in the treatment of esophageal and hepatic carcinoma for decades. For the purpose of enhancing the antitumor potency and reducing cytotoxicity of ORI, multiwalled carbon nanotubes functionalized with carboxylic group (MWCNTs-COOH) were used as ORI carrier. ORI was noncovalently encapsulated into (or onto) the functionalized carbon nanotubes (MWCNTs-ORI). The obtained MWCNTs-ORI has been characterized. The ORI loading efficiency in MWCNTs-COOH carrier was studied to be about 82.6% (w/w).In vitrocytotoxicity assay on MWCNTs-ORI gave IC50of7.29±0.5 μg/mL and ORI-F gave IC50of14.5±1.4 μg/mL. The antitumor effect studiesin vivoshowed that MWCNTs-ORI improved antitumor activity of ORI in comparison with ORI-F. The tumor inhibition ratio for MWCNTs-ORI (1.68×10-2 g·Kg−1·d−1) was 86.4%, higher than that of ORI-F (1.68×10-2 g·Kg−1·d−1) which was 39.2%. This can greatly improve the pharmaceutical efficiency and reduce potential side effects.

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In vitro cytotoxicity and induction of apoptosis by multiwalled carbon nanotubes in human peripheral lymphocytes: Correlation with physicochemical properties

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb11.4082 ◽

2012 ◽

Vol 11 (52) ◽

Author(s):

Mohamed F. Elshal

Keyword(s):

Carbon Nanotubes ◽

Physicochemical Properties ◽

Multiwalled Carbon Nanotubes ◽

In Vitro Cytotoxicity ◽

Multiwalled Carbon ◽

Peripheral Lymphocytes ◽

Human Peripheral Lymphocytes ◽

Induction Of Apoptosis

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Poly(3-Hydroxybutyrate)-Multiwalled Carbon Nanotubes Electrospun Scaffolds Modified with Curcumin

Polymers ◽

10.3390/polym12112588 ◽

2020 ◽

Vol 12 (11) ◽

pp. 2588

Author(s):

Nader Tanideh ◽

Negar Azarpira ◽

Najmeh Sarafraz ◽

Shahrokh Zare ◽

Aida Rowshanghiyas ◽

...

Keyword(s):

Carbon Nanotubes ◽

Tissue Engineering ◽

Multiwalled Carbon Nanotubes ◽

Inflammatory Reaction ◽

3D Structure ◽

Hydrolytic Degradation ◽

Multiwalled Carbon ◽

Electrospun Scaffolds

Appropriate selection of suitable materials and methods is essential for scaffolds fabrication in tissue engineering. The major challenge is to mimic the structure and functions of the extracellular matrix (ECM) of the native tissues. In this study, an optimized 3D structure containing poly(3-hydroxybutyrate) (P3HB), multiwalled carbon nanotubes (MCNTs) and curcumin (CUR) was created by electrospinning a novel biomimetic scaffold. CUR, a natural anti-inflammatory compound, has been selected as a bioactive component to increase the biocompatibility and reduce the potential inflammatory reaction of electrospun scaffolds. The presence of CUR in electrospun scaffolds was confirmed by 1H NMR and Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) revealed highly interconnected porosity of the obtained 3D structures. Addition of up to 20 wt% CUR has enhanced mechanical properties of the scaffolds. CUR has also promoted in vitro bioactivity and hydrolytic degradation of the electrospun nanofibers. The developed P3HB-MCNT composite scaffolds containing 20 wt% of CUR revealed excellent in vitro cytocompatibility using mesenchymal stem cells and in vivo biocompatibility in rat animal model study. Importantly, the reduced inflammatory reaction in the rat model after 8 weeks of implantation has also been observed for scaffolds modified with CUR. Overall, newly developed P3HB-MCNTs-CUR electrospun scaffolds have demonstrated their high potential for tissue engineering applications.

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Synthesis of Multiwalled Carbon Nanotubes–Poly(Methacrylic Acid) Nanohybrid Systems: Characterization, Thermal Properties, and In Vitro Release Studies of Naproxen as a Model Drug

Polymer-Plastics Technology and Engineering ◽

10.1080/03602559.2017.1289401 ◽

2017 ◽

Vol 56 (16) ◽

pp. 1723-1729 ◽

Cited By ~ 2

Author(s):

Abdolrahim Abbaszad Rafi ◽

Mohammad Karzar Jeddi ◽

Abdollah Bashir-Hashemi ◽

Mehrdad Mahkam

Keyword(s):

Carbon Nanotubes ◽

Thermal Properties ◽

Multiwalled Carbon Nanotubes ◽

Methacrylic Acid ◽

In Vitro Release ◽

Multiwalled Carbon ◽

Release Studies ◽

Model Drug ◽

Vitro Release

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Antagonistic effect of co-exposure to short-multiwalled carbon nanotubes and benzo[a]pyrene in human lung cells (A549)

Toxicology and Industrial Health ◽

10.1177/0748233719854570 ◽

2019 ◽

Vol 35 (6) ◽

pp. 445-456

Author(s):

Mansour Rezazadeh Azari ◽

Yousef Mohammadian ◽

Habibollah Peirovi ◽

Meisam Omidi ◽

Fariba Khodagholi ◽

...

Keyword(s):

Carbon Nanotubes ◽

Multiwalled Carbon Nanotubes ◽

Human Lung ◽

A549 Cells ◽

In Vitro Toxicity ◽

Ros Generation ◽

Exposure Condition ◽

Multiwalled Carbon ◽

Human Lung Cells ◽

Starting Point

In theenvironment, co-exposure to short-multiwalled carbon nanotubes (S-MWCNTs) and polycyclic aromatic compounds (PAHs) has been reported. In the co-exposure condition, the adsorption of PAHs onto MWCNTs may reduce PAHs toxic effect. The objective of this study was to investigate the cytotoxicity of S-MWCNTs and benzo[a]pyrene (B[a]P) individually, and in combination in human lung cell lines (A549). The adsorption of B[a]P onto MWCNTs was measured spectrometrically. In vitro toxicity was assessed through cell viability, reactive oxygen species (ROS) generation, apoptosis, and 8-hydroxy-2′-deoxyguanosine (8-OHdG) generation experiments. The S-MWCNTs demonstrated cytotoxicity through the generation of ROS, apoptosis, and 8-OHdG in A549 cells. Co-exposure to S-MWCNTs and B[a]P demonstrated a significant reduction in ROS generation and apoptosis compared with the sum of their separate toxic effects at the same concentrations. Decreasing the bioavailability of B[a]P by MWCNT interaction is the probable reason for the antagonistic effects of the co-exposure condition. The findings of this study will contribute to a better understanding of the health effects of co-exposures to air pollutants and could be a starting point for modifying future health risk assessments.

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