scholarly journals Poly(3-Hydroxybutyrate)-Multiwalled Carbon Nanotubes Electrospun Scaffolds Modified with Curcumin

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2588
Author(s):  
Nader Tanideh ◽  
Negar Azarpira ◽  
Najmeh Sarafraz ◽  
Shahrokh Zare ◽  
Aida Rowshanghiyas ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Tissue Engineering ◽  
Multiwalled Carbon Nanotubes ◽  
Inflammatory Reaction ◽  
3D Structure ◽  
Hydrolytic Degradation ◽  
Multiwalled Carbon ◽  
Electrospun Scaffolds

Appropriate selection of suitable materials and methods is essential for scaffolds fabrication in tissue engineering. The major challenge is to mimic the structure and functions of the extracellular matrix (ECM) of the native tissues. In this study, an optimized 3D structure containing poly(3-hydroxybutyrate) (P3HB), multiwalled carbon nanotubes (MCNTs) and curcumin (CUR) was created by electrospinning a novel biomimetic scaffold. CUR, a natural anti-inflammatory compound, has been selected as a bioactive component to increase the biocompatibility and reduce the potential inflammatory reaction of electrospun scaffolds. The presence of CUR in electrospun scaffolds was confirmed by 1H NMR and Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) revealed highly interconnected porosity of the obtained 3D structures. Addition of up to 20 wt% CUR has enhanced mechanical properties of the scaffolds. CUR has also promoted in vitro bioactivity and hydrolytic degradation of the electrospun nanofibers. The developed P3HB-MCNT composite scaffolds containing 20 wt% of CUR revealed excellent in vitro cytocompatibility using mesenchymal stem cells and in vivo biocompatibility in rat animal model study. Importantly, the reduced inflammatory reaction in the rat model after 8 weeks of implantation has also been observed for scaffolds modified with CUR. Overall, newly developed P3HB-MCNTs-CUR electrospun scaffolds have demonstrated their high potential for tissue engineering applications.

scholarly journals Preparation, Characterization, andIn VitroandVivoAntitumor Activity of Oridonin-Conjugated Multiwalled Carbon Nanotubes Functionalized with Carboxylic Group

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Chuanjin Wang ◽  
Wei Li
Keyword(s):  
Carbon Nanotubes ◽  
Multiwalled Carbon Nanotubes ◽  
Tumor Therapy ◽  
Water Soluble ◽  
Carboxylic Group ◽  
Multiwalled Carbon ◽  
Compound C ◽  
Poorly Water Soluble

Carbon nanotubes have shown great potential in tumor therapy. Oridonin (ORI) is a poorly water-soluble diterpenoid compound (C20H28O6) used in the treatment of esophageal and hepatic carcinoma for decades. For the purpose of enhancing the antitumor potency and reducing cytotoxicity of ORI, multiwalled carbon nanotubes functionalized with carboxylic group (MWCNTs-COOH) were used as ORI carrier. ORI was noncovalently encapsulated into (or onto) the functionalized carbon nanotubes (MWCNTs-ORI). The obtained MWCNTs-ORI has been characterized. The ORI loading efficiency in MWCNTs-COOH carrier was studied to be about 82.6% (w/w).In vitrocytotoxicity assay on MWCNTs-ORI gave IC50of7.29±0.5 μg/mL and ORI-F gave IC50of14.5±1.4 μg/mL. The antitumor effect studiesin vivoshowed that MWCNTs-ORI improved antitumor activity of ORI in comparison with ORI-F. The tumor inhibition ratio for MWCNTs-ORI (1.68×10-2 g·Kg−1·d−1) was 86.4%, higher than that of ORI-F (1.68×10-2 g·Kg−1·d−1) which was 39.2%. This can greatly improve the pharmaceutical efficiency and reduce potential side effects.

scholarly journals 3D meshes of carbon nanotubes guide functional reconnection of segregated spinal explants

2016 ◽  
Vol 2 (7) ◽  
pp. e1600087 ◽  
Author(s):  
Sadaf Usmani ◽  
Emily Rose Aurand ◽  
Manuela Medelin ◽  
Alessandra Fabbro ◽  
Denis Scaini ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
3D Structure ◽  
Three Dimensional ◽  
Significant Progress ◽  
Multiwalled Carbon ◽  
3D Mesh ◽  
Pure Carbon ◽  
The Brain

In modern neuroscience, significant progress in developing structural scaffolds integrated with the brain is provided by the increasing use of nanomaterials. We show that a multiwalled carbon nanotube self-standing framework, consisting of a three-dimensional (3D) mesh of interconnected, conductive, pure carbon nanotubes, can guide the formation of neural webs in vitro where the spontaneous regrowth of neurite bundles is molded into a dense random net. This morphology of the fiber regrowth shaped by the 3D structure supports the successful reconnection of segregated spinal cord segments. We further observed in vivo the adaptability of these 3D devices in a healthy physiological environment. Our study shows that 3D artificial scaffolds may drive local rewiring in vitro and hold great potential for the development of future in vivo interfaces.

scholarly journals Multiwalled carbon nanotubes for combination therapy: a biodistribution and efficacy pilot study

2019 ◽  
Vol 7 (16) ◽  
pp. 2678-2687 ◽  
Author(s):  
Giacomo Biagiotti ◽  
Federica Pisaneschi ◽  
Seth T. Gammon ◽  
Fabrizio Machetti ◽  
Maria Cristina Ligi ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Pilot Study ◽  
Combination Therapy ◽  
Multiwalled Carbon Nanotubes ◽  
Multiwalled Carbon

A doxorubicin/metformin carrier for chemotherapy based on oxidized MWCNTs. Efficacy in vitro and in vivo.

Synthesis and bioactivity of gelatin/multiwalled carbon nanotubes/hydroxyapatite nanofibrous scaffolds towards bone tissue engineering

RSC Advances ◽  
2015 ◽  
Vol 5 (66) ◽  
pp. 53550-53558 ◽  
Author(s):  
Hualin Wang ◽  
Chengjiang Chu ◽  
Ruizhi Cai ◽  
Suwei Jiang ◽  
Linfeng Zhai ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Multiwalled Carbon Nanotubes ◽  
Multiwalled Carbon ◽  
Nanofibrous Scaffolds

The in vitro bioactivity of scaffolds, and the adhesion, mineralization, viability and proliferation of hFOBs on gelatin/MWNTs/HA nanofibrous scaffolds.

scholarly journals Cisplatin loaded multiwalled carbon nanotubes reverse drug resistance in NSCLC by inhibiting EMT

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yuxin Qi ◽  
Wenping Yang ◽  
Shuang Liu ◽  
Fanjie Han ◽  
Haibin Wang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Carbon Nanotubes ◽  
Drug Resistance ◽  
Western Blot ◽  
Multiwalled Carbon Nanotubes ◽  
Cell Lines ◽  
Nude Mice ◽  
Expression Level ◽  
Multiwalled Carbon

Abstract Background Lung cancer is one of the important health threats worldwide, of which 5-year survival rate is less than 15%. Non-small-cell lung cancer (NSCLC) accounts for about 80% of all lung cancer with high metastasis and mortality. Methods Cisplatin loaded multiwalled carbon nanotubes (Pt-MWNTS) were synthesized and used to evaluate the anticancer effect in our study. The NSCLC cell lines A549 (cisplatin sensitive) and A549/DDP (cisplatin resistant) were used in our in vitro assays. MTT was used to determine Cancer cells viability and invasion were measured by MTT assay and Transwell assay, respectively. Apoptosis and epithelial-mesenchymal transition related marker proteins were measured by western blot. The in vivo anti-cancer effect of Pt-MWNTs were performed in male BALB/c nude mice (4-week old). Results Pt-MWNTS were synthesized and characterized by X-ray diffraction, Raman, FT-IR spectroscopy and scan electron microscopy. No significant cytotoxicity of MWNTS was detected in both A549/DDP and A549 cell lines. However, Pt-MWNTS showed a stronger inhibition effect on cell growth than free cisplatin, especially on A549/DDP. We found Pt-MWNTS showed higher intracellular accumulation of cisplatin in A549/DDP cells than free cisplatin and resulted in enhanced the percent of apoptotic cells. Western blot showed that application of Pt-MWNTS can significantly upregulate the expression level of Bax, Bim, Bid, Caspase-3 and Caspase-9 while downregulate the expression level of Bcl-2, compared with free cisplatin. Moreover, the expression level of mesenchymal markers like Vimentin and N-cadherin was more efficiently reduced by Pt-MWNTS treatment in A549/DDP cells than free cisplatin. In vivo study in nude mice proved that Pt-MWNTS more effectively inhibited tumorigenesis compared with cisplatin, although both of them had no significant effect on body weight. Conclusion Pt-MWNT reverses the drug resistance in the A549/DDP cell line, underlying its possibility of treating NSCLC with cisplatin resistance.

Hemostatic Effect of Aminated Multiwalled Carbon Nanotubes/Oxidized Regenerated Cellulose Nanocomposites

2019 ◽  
Vol 19 (11) ◽  
pp. 7410-7415 ◽  
Author(s):  
Baode Zhang ◽  
Ali Nabipour Chakoli ◽  
Jin Mei He ◽  
Yu Dong Huang ◽  
Andrey N. Aleshin
Keyword(s):  
Carbon Nanotubes ◽  
Multiwalled Carbon Nanotubes ◽  
Regenerated Cellulose ◽  
Oxidized Regenerated Cellulose ◽  
Multiwalled Carbon ◽  
Hemostatic Effect ◽  
Pristine Mwcnts ◽  
Ft Ir ◽  
Cellulose Nanocomposites

We have investigated the covalent conjugation of aminated multiwalled carbon nanotubes (MWCNTNH2)s with Oxidized Regenerated Cellulose (ORC) in order to enhance the hemostatic effect. The MWCNT-NH2s were prepared by functionalization of pristine MWCNTs (pMWCNTs) using amine groups. Neat ORC gauze and MWCNT-NH2s were reacted using glutamic acid as cross linking bridge. We investigated an amination of pMWCNTs as well as the dispersion of MWCNT-NH2s in the ORC gauze as matrix and their interfacial interactions by SEM and FT-IR. The results revealed that relatively strong interaction exists between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results in the significant increment of water uptake of MWCNT-NH2s/ORC composites with increasing the concentration of MWCNT-NH2s in composite. The in-vitro procoagulation test shows that the MWCNT-NH2s/ORC gauzes have significant procoagulant activity. The hemostatic evaluation of MWCNT-NH2s/ORC composites on rabbits shows that the aminated MWCNTs increase the rate of blood stopping and hence they decrease the blood loosing from injured sites. Hemostatic evaluation indicates that the MWCNT-NH2s/ORC gauze has a valuable hemostatic performance. The products of platelets release reaction, activated platelets glycoprotein and activated clotting enzymes were increased simultaneously. The mechanism of the hemostasis for MWCNT-NH2s/ORC gauze is discussed.

scholarly journals Basic Potential of Carbon Nanotubes in Tissue Engineering Applications

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Hisao Haniu ◽  
Naoto Saito ◽  
Yoshikazu Matsuda ◽  
Tamotsu Tsukahara ◽  
Yuki Usui ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Tissue Engineering ◽  
High Surface ◽  
High Surface Area ◽  
Volume Ratio ◽  
Medical Application ◽  
Mechanical And Thermal Properties ◽  
Biological Responses

Carbon nanotubes (CNTs) are attracting interest in various fields of science because they possess a high surface area-to-volume ratio and excellent electronic, mechanical, and thermal properties. Various medical applications of CNTs are expected, and the properties of CNTs have been greatly improved for use in biomaterials. However, the safety of CNTs remains unclear, which impedes their medical application. Our group is evaluating the biological responses of multiwall CNTs (MWCNTs)in vivoandin vitrofor the promotion of tissue regeneration as safe scaffold materials. We recently showed that intracellular accumulation is important for the cytotoxicity of CNTs, and we reported the active physiological functions CNTs in cells. In this review, we describe the effects of CNTsin vivoandin vitroobserved by our group from the standpoint of tissue engineering, and we introduce the findings of other research groups.

scholarly journals Full cell infiltration and thick tissue formation in vivo in tailored electrospun scaffolds

2020 ◽  
Author(s):  
Jip Zonderland ◽  
Silvia Rezzola ◽  
David Gomes ◽  
Sandra Camarero Espinosa ◽  
Ana Henriques Ferreira Lourenço ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fiber Diameter ◽  
Cell Infiltration ◽  
Tissue Formation ◽  
Cell Seeding ◽  
Subcutaneous Implantation ◽  
Electrospun Scaffolds ◽  
Limited Depth

AbstractElectrospun (ESP) scaffolds are a promising type of tissue engineering constructs for large defects with limited depth. To form new functional tissue, the scaffolds need to be infiltrated with cells, which will deposit extracellular matrix. However, due to dense fiber packing and small pores, cell and tissue infiltration of ESP scaffolds is limited. Here, we combine two established methods, increasing fiber diameter and co-spinning sacrificial fibers, to create a porous ESP scaffold that allows robust tissue infiltration. Full cell infiltration across 2 mm thick scaffolds is seen 3 weeks after subcutaneous implantation in rats. After 6 weeks, the ESP scaffolds are almost fully filled with de novo tissue. Cell infiltration and tissue formation in vivo in this thickness has not been previously achieved. In addition, we propose a novel method for in vitro cell seeding to improve cell infiltration and a model to study 3D migration through a fibrous mesh. This easy approach to facilitate cell infiltration further improves previous efforts and could greatly aid tissue engineering approaches utilizing ESP scaffolds.Statement of significanceElectrospinning creates highly porous scaffolds with nano- to micrometer sized fibers and are a promising candidate for a variety of tissue engineering applications. However, smaller fibers also create small pores which are difficult for cells to penetrate, restricting cells to the top layers of the scaffolds. Here, we have improved the cell infiltration by optimizing fiber diameter and by co-spinning a sacrificial polymer. We developed novel culture technique that can be used to improve cell seeding and to study cytokine driven 3D migration through fibrous meshes. After subcutaneous implantation, infiltration of tissue and cells was observed up to throughout up to 2 mm thick scaffolds. This depth of infiltration in vivo had not yet been reported for electrospun scaffolds. The scaffolds we present here can be used for in vitro studies of migration, and for tissue engineering in defects with a large surface area and limited depth.

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