Fluorescent Assays To Quantitate Enzymatic Activities Yielding as End Product an Aqueous-Insoluble Indigo-Blue Dye

Langmuir ◽  
2004 ◽  
Vol 20 (6) ◽  
pp. 2424-2428 ◽  
Author(s):  
Komandoor E. Achyuthan
Keyword(s):  
Enzymatic Activities ◽  
Blue Dye ◽  
Indigo Blue

scholarly journals Evaluation and Predictive Modeling of Removal Condition for Bioadsorption of Indigo Blue Dye by Spirulina platensis

2020 ◽  
Vol 8 (1) ◽  
pp. 82 ◽  
Author(s):  
Robledo-Padilla ◽  
Aquines ◽  
Silva-Núñez ◽  
Alemán-Nava ◽  
Castillo-Zacarías ◽  
...  
Keyword(s):  
Spirulina Platensis ◽  
Biomass Concentration ◽  
Blue Dye ◽  
Removal Capacity ◽  
Treatment Conditions ◽  
Linear Behavior ◽  
Indigo Blue ◽  
Experimental Values ◽  
Physical Treatments ◽  
Similar Accuracy

Among the different chemical and physical treatments used to remove the color of the textile effluents, bioremediation offers many benefits to the environment. In this study, we determined the potential of Spirulina platensis (S. platensis) for decolorizing indigo blue dye under different incubation conditions. The microalgae were incubated at different pH (from 4 to 10) to calibrate for the optimal discoloration condition; a pH of 4 was found to be optimal. The biomass concentration in all experiments was 1 g/L, which was able to decolorize the indigo blue dye by day 3. These results showed that S. platensis is capable of removing indigo blue dye at low biomass. However, this was dependent on the treatment conditions, where temperature played the most crucial role. Two theoretical adsorption models, namely (1) a first-order model equation and (2) a second-order rate equation, were compared with observed adsorption vs. time curves for different initial concentrations (from 25 to 100 mg/L). The comparison between models showed similar accuracy and agreement with the experimental values. The observed adsorption isotherms for three temperatures (30, 40, and 50 °C) were plotted, showing fairly linear behavior in the measured range. The adsorption equilibrium isotherms were estimated, providing an initial description of the dye removal capacity of S. platensis.

Evaluation of operational parameters from a microfiltration system for indigo blue dye recovery from textile dye effluent

2013 ◽  
Vol 52 (1-3) ◽  
pp. 257-266 ◽  
Author(s):  
Míriam Cristina Santos Amaral ◽  
Luzia Sergina França Neta ◽  
Mariana Souza ◽  
Naiara Cerqueira ◽  
Roberto Bentes de Carvalho
Keyword(s):  
Blue Dye ◽  
Textile Dye ◽  
Operational Parameters ◽  
Textile Dye Effluent ◽  
Indigo Blue

scholarly journals Electrocoagulation Applied for Textile Dye Oxidation Using Iron Slag as Electrodes

2021 ◽  
Author(s):  
Rafaela De Maman ◽  
Vilson Conrado da Luz ◽  
Laura Behling ◽  
Adriana Dervanoski ◽  
Clarissa Dalla Rosa ◽  
...  
Keyword(s):  
Textile Industry ◽  
Oxygen Demand ◽  
Electrochemical Process ◽  
Blue Dye ◽  
Textile Dye ◽  
Phenol Removal ◽  
Good Efficiency ◽  
Iron Slag ◽  
Indigo Blue ◽  
Textile Factory

Abstract The Indigo Blue dye is widely used in the textile industry, specifically in jeans dyeing, the effluents of which, rich in organic pollutants with recalcitrant characteristics, end up causing several environmental impacts, requiring efficient treatments. Several pieces of research have been conducted in search of effective treatment methods, among which is electrocoagulation. This treatment consists of an electrochemical process that generates its own coagulant by applying electric current on metallic electrodes, bypassing the use of other chemical products. The objective of this work was to evaluate the potential use of iron slag in the electrocoagulation of a synthetic effluent containing commercial dye Indigo Blue and the effluent from a textile factory. The quantified parameters were color, turbidity, pH, electrical conductivity, sludge generation, phenol removal, chemical oxygen demand (COD), and total organic carbon (TOC). The electrocoagulation treatment presented a good efficiency in removing the analyzed parameters, obtaining average removal in the synthetic effluent of 85 % of color and 100 % of phenol after 25 min of electrolysis. For the effluent from the textile factory, average reductions of 80 % of color, 91 % of turbidity, 100 % of phenol, 55 % of COD, and 73 % of TOC were measured after 60 min of electrolysis. The results obtained demonstrate the potential of using iron slag as an electrode in the electrocoagulation process in order to reuse industrial waste and reduce costs in the treatment and disposal of solid waste.

scholarly journals Comparative investigation of indigo blue dye removal efficiency of activated carbon and natural clay in adsorption/ultrafiltration system

2019 ◽  
Vol 164 ◽  
pp. 326-338
Author(s):  
M. Missaoui ◽  
N. Tahri ◽  
M.O. Daramola ◽  
J. Duplay ◽  
G. Schäfer ◽  
...  
Keyword(s):  
Activated Carbon ◽  
Removal Efficiency ◽  
Dye Removal ◽  
Comparative Investigation ◽  
Blue Dye ◽  
Natural Clay ◽  
Indigo Blue

scholarly journals Adsorption Isotherms studies of the removal of Indigo Blue dye from aqueous solution using chemically modified coconut shell

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Olaniyi K Yusuff ◽  
Adenike M. O Abdulraheem ◽  
Omowumi D. Agboola
Keyword(s):  
Aqueous Solution ◽  
Adsorption Process ◽  
Dye Adsorption ◽  
Coconut Shell ◽  
Isotherm Models ◽  
Blue Dye ◽  
Chemically Modified ◽  
Equilibrium Data ◽  
Indigo Blue ◽  
Adsorption Equilibrium Data

Introduction: The conversion and utilization of agricultural wastes in environmentally friendly processes have transformed these materials into useful rather than waste materials. Aim: This study investigates the adsorption of indigo blue dye from aqueous solution onto coconut shell, a low cost agricultural waste material in a batch process. Materials and Methods: Pulverized coconut shell was chemically modified and characterized using the Fourier Transform Infra Red spectroscopy and Scanning Electron Microscopy. Adsorption process using the chemically modified coconut shell was studied as a function of pH, initial dye concentration, adsorbent dose, and contact time. The adsorption equilibrium data were analyzed with Langmuir, Freundlich and Temkin isotherm models. Results: The results revealed that percentage of the indigo dye adsorbed from aqueous solution varied linearly with the adsorbent dose, concentration and time with maximum percentage dye adsorption of 88.4% at 70 mg dosage, 95.8% at 0.05 mg/L concentration and 90% at 1 hr contact time but varies non-linearly with pH with maximum percentage dye adsorption of 92.9% attained at pH of 5. The adsorption equilibrium data were analyzed with Langmuir, Freundlich and Temkin isotherm models with the Langmuir isotherm having the best fit to the adsorption process with R2 value of 0.998. The experimental data were best described by the pseudo-second order kinetics model. FTIR analyses reveal that the adsorption process was through a chemical interaction of the dye with some functional groups at the surface of the adsorbent Conclusion: The chemically modified coconut shell is an effective adsorbent for the removal of indigo dye from aqueous solution is by chemisorption process with the adsorbent surface energetically homogeneous (n < 1). Keywords: Adsorption, Indigo blue dye, Coconut Shell, Kinetics, Aqueous solution.

First experiences with a new radiopharmaceutical for sentinel lymph node detection in malignant melanoma

2004 ◽  
Vol 43 (01) ◽  
pp. 10-15 ◽  
Author(s):  
R. A. Schmid ◽  
C. Kunte ◽  
B. Konz ◽  
K. Hahn ◽  
M. Weiss
Keyword(s):  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Malignant Melanoma ◽  
Sentinel Node ◽  
Gamma Probe ◽  
Blue Dye ◽  
Sentinel Nodes ◽  
Technetium 99M ◽  
Positive Rate ◽  
Metastatic Involvement

Summary Aim of this study was to localize the sentinel lymph node by lymphoscintigraphy using technetium-99m colloidal rhenium sulphide (Nanocis®), a new commercially available radiopharmaceutical. Due to the manufacturers’ instructions it is licensed for lymphoscintigraphy. Patients, methods: 35 consecutive patients with histologically proved malignant melanoma, but without clinical evidence of metastases, were preoperatively examined by injecting 20-40 MBq Nanocis® with (mean particle size: 100 nm; range: 50-200 nm) intradermally around the lesion. Additionally blue dye was injected intaoperatively. A hand-held gamma probe guided sentinel node biopsy. Results: During surgery, the preoperatively scintigraphically detected sentinel lymph nodes were identified in 34/35 (97%) patients. The number of sentinel nodes per patient ranged from one to four (mean: n = 1.8). Histologically, metastatic involvement of the sentinel lymph node was found in 12/35 (34%) patients; the sentinel lymph node positive-rate (14/63 SLN) was 22%. Thus, it is comparable to the findings of SLN-mapping using other technetium-99m-labeled nanocolloides. Conclusion: 99mTc-bound colloidal rhenium sulphide is also suitable for sentinel node mapping.

Using of modified-bentonite as low-cost sorbent for removal of methylene blue dye from aqueous solution

2020 ◽  
Vol 27 (2) ◽  
pp. 45-54
Author(s):  
Saraa Muwafaq Ibrahim ◽  
Ziad T. Abd Ali
Keyword(s):  
Aqueous Solution ◽  
Methylene Blue ◽  
Silanol Group ◽  
Infrared Spectrometry ◽  
Blue Dye ◽  
Ammonium Bromide ◽  
Vibration Band ◽  
Order Kinetic ◽  
Methylene Blue Dye ◽  
Modified Bentonite

Batch experiments have been studied to remove methylene blue dye (MB) from aqueous solution using modified bentonite. The modified bentonite was synthesized by replacing exchangeable calcium cations in natural bentonite with cationic surfactant cetyl trimethyl ammonium bromide (CTAB). The characteristics of modified bentonite were studied using different analysis such as Scanning electronic microscopy (SEM), Fourier transform infrared spectrometry (FTIR) and surface area. Where SEM shows the natural bentonite has a porous structure, a rough and uneven appearance with scattered and different block structure sizes, while the modified bentonite surface morphology was smooth and supplemented by a limited number of holes. On other hand, (FTIR) analysis that proved NH group aliphatic and aromatic group of MB and silanol group are responsible for the sorption of contaminate. The organic matter peaks at 2848 and 2930 cm-1 in the spectra of modified bentonite which are sharper than those of the natural bentonite were assigned to the CH2 scissor vibration band and the symmetrical CH3 stretching absorption band, respectively, also the 2930 cm-1 peak is assigned to CH stretching band. The batch study was provided the maximum removal efficiency (99.99 % MB) with a sorption capacity of 129.87 mg/g at specified conditions (100 mg/L, 25℃, pH 11 and 250rpm). The sorption isotherm data fitted well with the Freundlich isotherm model. The kinetic studies were revealed that the sorption follows a pseudo-second-order kinetic model which indicates chemisorption between sorbent and sorbate molecules.

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