New Method for the Quantitative Determination of Major Protein Carbonyls, α-Aminoadipic and γ-Glutamic Semialdehydes:  Investigation of the Formation Mechanism and Chemical Nature in Vitro and in Vivo

2006 ◽  
Vol 19 (8) ◽  
pp. 1059-1065 ◽  
Author(s):  
Mitsugu Akagawa ◽  
Daisuke Sasaki ◽  
Yoshihisa Ishii ◽  
Yayoi Kurota ◽  
Mari Yotsu-Yamashita ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Formation Mechanism ◽  
Chemical Nature ◽  
New Method ◽  
Protein Carbonyls ◽  
Major Protein

Pegylated magnetic nanocarriers for doxorubicin delivery: A quantitative determination of stealthiness in vitro and in vivo

2012 ◽  
Vol 81 (3) ◽  
pp. 498-505 ◽  
Author(s):  
E. Allard-Vannier ◽  
S. Cohen-Jonathan ◽  
J. Gautier ◽  
K. Hervé-Aubert ◽  
E. Munnier ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Doxorubicin Delivery ◽  
Magnetic Nanocarriers

scholarly journals A Newly Developed Sublingual Tonometric Method for the Evaluation of Tissue Perfusion and Its ValidationIn Vitroand in Healthy PersonsIn Vivoand the Results of the Measurements in COPD Patients

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Zoltán Rózsavölgyi ◽  
Domokos Boda ◽  
Andrea Hajnal ◽  
Krisztina Boda ◽  
Attila Somfay
Keyword(s):  
Stable Condition ◽  
Tissue Perfusion ◽  
New Method ◽  
Actual Condition ◽  
Gastric Tonometry ◽  
Copd Patients ◽  
Mucosal Perfusion

Introduction. Since its first publication in the medical literature, an extremely large number of references have demonstrated that the tonometric measurement of tissue perfusion is a reliable indicator of the actual condition of critically ill patients. Later a new method was developed by the introduction of sublingual tonometry for the determination of tissue perfusion. In comparison with gastric tonometry, the new method was simpler and could even be used in awake patients. Unfortunately, at present, because of severe failures of manufacturing, the device is withdrawn from commerce.Materials and Methods. In this study, we present a new method using a newly developed tool for the PslCO2measurement in sublingual tonometry as well as the data for its validationin vitroandin vivoand the results of 25 volunteers and 54 COPD patients belonging to different GOLD groups at their hospitalization due to the acute exacerbation of the disease but already in a stable condition at the time of the examination.Results and Conclusion. The results of the performed examinations showed that the method is suitable for monitoring the actual condition of the patients by mucosal perfusion tonometry in the sublingual region.

scholarly journals Quantitative determination of adenovirus-mediated gene delivery to rat cardiac myocytes in vitro and in vivo.

1993 ◽  
Vol 90 (24) ◽  
pp. 11498-11502 ◽  
Author(s):  
A. Kass-Eisler ◽  
E. Falck-Pedersen ◽  
M. Alvira ◽  
J. Rivera ◽  
P. M. Buttrick ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Quantitative Determination ◽  
Cardiac Myocytes ◽  
Rat Cardiac Myocytes

A Molecular Approach to Immunoscintigraphy: A Study of the T-Antigen Conformation on the Surface of Tumors

1987 ◽  
Vol 26 (01) ◽  
pp. 1-6 ◽  
Author(s):  
S. Selvaraj ◽  
M. R. Suresh ◽  
G. McLean ◽  
D. Willans ◽  
C. Turner ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tumor Cell ◽  
T Antigen ◽  
Human Carcinomas ◽  
Animal Tumor ◽  
Synthetic Antigens

The role of glycoconjugates in tumor cell differentiation has been well documented. We have examined the expression of the two anomers of the Thomsen-Friedenreich antigen on the surface of human, canine and murine tumor cell membranes both in vitro and in vivo. This has been accomplished through the synthesis of the disaccharide terminal residues in both a and ß configuration. Both entities were used to generate murine monoclonal antibodies which recognized the carbohydrate determinants. The determination of fine specificities of these antibodies was effected by means of cellular uptake, immunohistopathology and immunoscintigraphy. Examination of pathological specimens of human and canine tumor tissue indicated that the expressed antigen was in the β configuration. More than 89% of all human carcinomas tested expressed the antigen in the above anomeric form. The combination of synthetic antigens and monoclonal antibodies raised specifically against them provide us with invaluable tools for the study of tumor marker expression in humans and their respective animal tumor models.

Storage of Human Blood Platelets

1974 ◽  
Vol 32 (02/03) ◽  
pp. 405-416 ◽  
Author(s):  
M. R Hardeman ◽  
Carina J L. Heynens
Keyword(s):  
Storage Temperature ◽  
Platelet Rich Plasma ◽  
Blood Platelets ◽  
Storage Period ◽  
Serotonin Uptake ◽  
Hypotonic Shock ◽  
Glycolytic Intermediates

SummaryStorage experiments were performed at 4°, 25° and 37° C with platelet-rich plasma under sterile conditions. In some experiments also the effect of storing platelets at 4° C in whole blood was investigated.Before, during and after three days of storage, the platelets were tested at 37° C for their serotonin uptake and response to hypotonic shock. In addition some glycolytic intermediates were determined.A fair correlation was noticed between the serotonin uptake and hypotonic shock experiments. Both parameters were best maintained at 25° C. Also platelet counting, performed after the storage period, indicated 25° C as the best storage temperature. Determination of glycolytic intermediates did not justify any conclusion regarding the optimal storage temperature. Of the various anticoagulants studied, ACD and heparin gave the best results as to the serotonin uptake and hypotonic shock response, either with fresh or stored platelets. The use of EDTA resulted in the lowest activity, especially after storage.The results of these storage experiments in vitro, correspond well with those in vivo reported in the literature.

AGGLUTINATION INHIBITION REACTIONS FOR THE DETERMINATION OF GONADOTROPHINS

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S95-S112 ◽  
Author(s):  
A. H. W. M. Schuurs
Keyword(s):  
Immune System ◽  
Quantitative Determination ◽  
Haemagglutination Inhibition ◽  
Latex Agglutination ◽  
Literature Survey ◽  
New Method ◽  
Test Fluid ◽  
Main Application ◽  
Test Systems

ABSTRACT Various techniques for sensitising erythrocytes and latex particles with gonadotrophins, particularly with HCG, are described. The haemagglutination inhibition reactions are generally interpreted by means of »erythrocyte settling patterns«. By a new method of evaluating these patterns a relatively precise quantitative determination is possible. Latex agglutination inhibition reactions on slides are particularly suitable as rapid qualitative tests. In cases where the maximum attainable sensitivity of the agglutination inhibition tests is insufficient, e. g. for determining LH concentrations in urine, the hormone in the test fluid has to be concentrated or extracted. An alternative method is a modified haemagglutination inhibition test for large volumes which is applicable to unconcentrated urine. Due to non-specific inhibitions the above-mentioned tests cannot be applied to unprocessed serum. Agglutination inhibition tests with HCG are already well advanced, pregnancy diagnosis being their main application. Now that highly purified HCG is available, a satisfactory specificity for these tests can be attained. If the immune system for HCG is used for estimating LH, it has to meet additional specificity requirements. Furthermore, the measure of cross-reaction and the choice of standard merit special attention. Finally, a literature survey is given of test systems in which LH and FSH were used as antigens.

Validation of Doxepin Quantitative Determination Methods for their Application to In Vitro, Ex Vivo and In Vivo Studies

2015 ◽  
Vol 11 (4) ◽  
pp. 269-277 ◽  
Author(s):  
Roser Sanz ◽  
Beatriz Clares ◽  
Mireia Mallandrich ◽  
Isidre Casals ◽  
David Bellido ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Ex Vivo ◽  
Determination Methods

Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fusobacterium Nucleatum ◽  
Living Cells ◽  
First Time ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

Sign in / Sign up

Export Citation Format

Share Document