Integrated control of Boophilus microplus ticks in Cuba based on vaccination with the anti-tick vaccine GavacTM

AbstractTicks are important ectoparasites of domestic and wild animals, and tick infestations economically impact cattle production worldwide. Control of cattle tick infestations has been primarily by application of acaricides which has resulted in selection of resistant ticks and environmental pollution. Herein we discuss data from tick vaccine application in Australia, Cuba, Mexico and other Latin American countries. Commercial tick vaccines for cattle based on the Boophilus microplus Bm86 gut antigen have proven to be a feasible tick control method that offers a cost-effective, environmentally friendly alternative to the use of acaricides. Commercial tick vaccines reduced tick infestations on cattle and the intensity of acaricide usage, as well as increasing animal production and reducing transmission of some tick-borne pathogens. Although commercialization of tick vaccines has been difficult owing to previous constraints of antigen discovery, the expense of testing vaccines in cattle, and company restructuring, the success of these vaccines over the past decade has clearly demonstrated their potential as an improved method of tick control for cattle. Development of improved vaccines in the future will be greatly enhanced by new and efficient molecular technologies for antigen discovery and the urgent need for a tick control method to reduce or replace the use of acaricides, especially in regions where extensive tick resistance has occurred.

Download Full-text

Immune Response of Naïve Cattle to Successive Infestations of Boophilus microplus Ticks

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.2000.tb05353.x ◽

2006 ◽

Vol 916 (1) ◽

pp. 650-653

Author(s):

LYGIA M.F. PASSOS ◽

OSVALDO ROSSETTI ◽

ALICIA ARESE ◽

CARLOS EDDI ◽

JORGE CARACOSTANTOGOLO ◽

...

Keyword(s):

Immune Response ◽

Boophilus Microplus ◽

Boophilus Microplus Ticks

Download Full-text

First Molecular Characterization of Anaplasma marginale in Cattle and Rhipicephalus (Boophilus) microplus Ticks in Cebu, Philippines

Journal of Veterinary Medical Science ◽

10.1292/jvms.12-0268 ◽

2013 ◽

Vol 75 (1) ◽

pp. 27-36 ◽

Cited By ~ 22

Author(s):

Adrian Patalinghug YBAÑEZ ◽

Thillaiampalam SIVAKUMAR ◽

Rochelle Haidee Daclan YBAÑEZ ◽

Jowarren Catingan RATILLA ◽

Zandro Obligado PEREZ ◽

...

Keyword(s):

Molecular Characterization ◽

Anaplasma Marginale ◽

Boophilus Microplus ◽

Boophilus Microplus Ticks

Download Full-text

Vaccine Efficacy of Bm86 Ortholog ofH. a. anatolicum, rHaa86 Expressed in Prokaryotic Expression System

Journal of Parasitology Research ◽

10.1155/2009/165812 ◽

2009 ◽

Vol 2009 ◽

pp. 1-7 ◽

Cited By ~ 6

Author(s):

P. Azhahianambi ◽

D. D. Ray ◽

Pallab Chaudhuri ◽

Rohita Gupta ◽

Srikanta Ghosh

Keyword(s):

Vaccine Efficacy ◽

Prokaryotic Expression ◽

Integrated Control ◽

Expression System ◽

Fusion Tag ◽

E Coli ◽

Adult Females ◽

Prokaryotic Expression System ◽

Integrated Tick Management ◽

Tick Vaccine

The use of tick vaccine in controlling ticks and tick borne diseases has been proved effective in integrated tick management format. For the control ofH. a. anatolicum, Bm86 ortholog ofH. a. anatolicumwas cloned and expressed as fusion protein inE. coliasE. coli-pETHaa86. The molecular weight of the rHaa86 was 97 kDa with a 19 kDa fusion tag of thioredoxin protein. The expressed protein was characterized immunologically and vaccine efficacy was evaluated. After 120 hours of challenge, only 26% tick could successfully fed on immunized animals. Besides significant reduction in feeding percentages, a significant reduction of 49.6 mg;P<.01in the weight of fed females in comparison to the females fed on control animals was recorded. Following oviposition, a significant reduction of 68.1 mg;P<.05in the egg masses of ticks fed on immunized animals in comparison to the ticks fed on control animals was noted. The reduction of number of females, mean weight of eggs, adult females and efficacy of immunogen were 73.8%, 31.3%, 15.8%, and 82.3%, respectively. The results indicated the possibility of development of rHaa86 based vaccine as a component of integrated control of tick species.

Download Full-text

Distribución de garrapatas Rhipicephalus (Boophilus) microplus en bovinos y fincas del Altiplano cundiboyacense (Colombia)

Ciencia y Tecnología Agropecuaria ◽

10.21930/rcta.vol11_num1_art:197 ◽

2010 ◽

Vol 11 (1) ◽

pp. 73 ◽

Cited By ~ 9

Author(s):

Jesús Alfredo Cortés Vecino ◽

Jesús Antonio Betancourt Echeverri ◽

Jorge Argüelles Cárdenas ◽

Luz Astrid Pulido Herrera

Keyword(s):

Population Dynamics ◽

Sea Level ◽

Potential Risk ◽

Boophilus Microplus ◽

Future Research ◽

First Report ◽

Boophilus Microplus Ticks

En Colombia, la presencia de la garrapata Rhipicephalus (Boophilus) microplus ha sido registrada en altitudes que oscilan entre 0 y 2.600 metros sobre el nivel del mar (msnm). Actualmente, no existen reportes de distribución de esta especie en el Altiplano cundiboyacense, zona geográfica de importancia nacional en la producción de leche. Para el presente trabajo se buscaron especímenes de cada uno de los estadios de la garrapata en bovinos y en potreros de 108 predios situados entre los 2.000 y 3.000 msnm, en las diferentes zonas del Altiplano cundiboyacense. Se colectaron 851 garrapatas, en total, de la especie R. (B.) microplus (100% de las muestras) en 34 predios ubicados entre los 1.966 y 2.903 msnm Este es el primer estudio en Colombia que registra la presencia de esta garrapata a una altitud superior a los 2.600 msnm y que caracteriza su distribución en el Altiplano cundiboyacense. Los resultados obtenidos demuestran la existencia de esta especie, ectoparásito de bovinos y vector de hemoparásitos, en zonas geográficas donde hasta hace algunos años no se registraba su presencia. Lo anterior plantea un panorama de riesgo para la presentación de babesiosis y anaplasmosis bovina en poblaciones altamente susceptibles. Estudios posteriores deberán definir la bioecología de esta garrapata en esta región y la prevalencia de infección en bovinos de los patógenos asociados. Distribution of Rhipicephalus (Boophilus) microplus Ticks on Cattle and Farms from Altiplano Cundiboyacense (Colombia)In Colombia, the presence of Rhipicephalus (Boophilus) microplus ticks has been registered in altitudes ranging from zero to 2,600 meters above the sea level. Currently, there are no reports of its distribution in the Altiplano Cundiboyacense, an important dairy region of Colombia. In the present work, ticks of each stage were searched on cattle and pastures from 108 farms ranging from 2,000 to 3,000 meters of altitude. A total of 851 specimens of R. (B.) microplus (100% of total) were collected from 34 farms located between 1,966 and 2,903 meters of altitude. This is the first report in Colombia on the presence of this tick in altitudes higher than 2,600 meters and on its distribution in the Altiplano Cundiboyacense. These results confirm the existence of this tick as an ectoparasite of cattle, vector of haemoparasites, in areas where it was no previously reported, and they present a potential risk of transmission of these pathogens in highly susceptible bovine populations. Future research must define the bioecology and population dynamics of this tick in that region and the prevalence of cattle infected with tick-borne pathogens.

Download Full-text

Resistance of cattle to cattle tick, Boophilus microplus. I. The development of resistance to experimental infestation

Australian Journal of Agricultural Research ◽

10.1071/ar9680323 ◽

1968 ◽

Vol 19 (2) ◽

pp. 323 ◽

Cited By ~ 16

Author(s):

RW Hewetson ◽

JV Nolan

Keyword(s):

Adult Female ◽

Egg Laying ◽

Boophilus Microplus ◽

Cattle Tick ◽

Development Of Resistance ◽

Experimental Infestation ◽

Boophilus Microplus Ticks

The development of a resistance by cattle to artificial infestations with Boophilus microplus ticks is reported and the sequence of development discussed. There was a regression of adult female ticks becoming replete with infestation (regression y = 2399.41 – 574x ± 54) up to the fourth infestation. The weight of replete female ticks dropped from resistant cattle increased with infestation but this effect is confounded with the effects of climate. The day of modal drop of replete females increased with infestation, which indicated an effect of resistance on modal drop. Egg-laying was not affected by a developing resistance, but the fertility of eggs from female egg-laying ticks was. There was an increase in fertility of eggs laid after the second infestation.

Download Full-text