DNA Microarrays Detect 4-Nonylphenol-induced Alterations in Gene Expression During Zebrafish Early Development

Genetic data from humans and mice reveal that the formation of cementum is sensitive to intra- and extracellular phosphate/pyrophosphate distribution. The intracellular molecular pathways whereby altered levels of extracellular phosphate concentration may affect cementum formation have not been elucidated. To initiate inquiry, we have studied the temporal effects of extracellular phosphate on global patterns of gene expression in a line of immortalized mouse cementoblasts. Total RNA from cultured cementoblasts treated with 5 mM inorganic phosphate over a designated time period, from 1–48 hrs, was analyzed for global patterns of gene expression by means of DNA microarrays representing the complete mouse genome. Analyses of significant hybridization signals indicated that 5 mM extracellular phosphate alters the expression of genes comprising several gene ontology (GO) groups, including transcription factor activity and Wnt signaling.

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High-Throughput Analysis of Gene Expression by Cutting-Edge Technology—DNA Microarrays (Gene Chips)

Gene Biotechnology ◽

10.1201/b10919-23 ◽

2016 ◽

pp. 459-468

Keyword(s):

Gene Expression ◽

High Throughput ◽

Dna Microarrays ◽

Cutting Edge ◽

High Throughput Analysis ◽

Throughput Analysis ◽

Gene Chips

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A Combinatorial Approach to Gene Expression Analysis: DNA Microarrays Lucia Altucci and Alessandro Weisz

Combinatorial Chemistry and Technologies ◽

10.1201/9781420027846-27 ◽

2005 ◽

pp. 555-582

Keyword(s):

Gene Expression ◽

Expression Analysis ◽

Dna Microarrays ◽

Gene Expression Analysis ◽

Combinatorial Approach

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Alkaloid Biosynthesis in the Early Stages of the Germination of Argemone mexicana L. (Papaveraceae)

Plants ◽

10.3390/plants10102226 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2226

Author(s):

Jorge Xool-Tamayo ◽

Yahaira Tamayo-Ordoñez ◽

Miriam Monforte-González ◽

José Armando Muñoz-Sánchez ◽

Felipe Vázquez-Flota

Keyword(s):

Gene Expression ◽

Tissue Distribution ◽

Early Development ◽

Transcriptional Activity ◽

Seedling Development ◽

Transport Systems ◽

Alkaloid Biosynthesis ◽

Argemone Mexicana ◽

Early Stages ◽

Low Levels

The synthesis of the benzylisoquinoline alkaloids, sanguinarine and berberine, was monitored in Argemone mexicana L. (Papaveracea) throughout the early stages of its hypocotyl and seedling development. Sanguinarine was detected in the cotyledons right after hypocotyl emergence, and it increased continuously until the apical hook unbent, prior to the cotyledonary leaves unfolding, when it abruptly fell. In the cotyledonary leaves, it also remained at low levels. Throughout development, berberine accumulation required the formation of cotyledonary leaves, whereas it was quickly detected in the hypocotyl from the time it emerged. Interestingly, the alkaloids detected in the cotyledons could have been imported from hypocotyls, because no transcriptional activity was detected in there. However, after turning into cotyledonary leaves, important levels of gene expression were noted. Taken together, these results suggest that the patterns of alkaloid tissue distribution are established from very early development, and might require transport systems.

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miRNAome expression profiles in the gonads of adultMelopsittacus undulatus

PeerJ ◽

10.7717/peerj.4615 ◽

2018 ◽

Vol 6 ◽

pp. e4615 ◽

Cited By ~ 5

Author(s):

Lan Jiang ◽

Qingqing Wang ◽

Jue Yu ◽

Vinita Gowda ◽

Gabriel Johnson ◽

...

Keyword(s):

Gene Expression ◽

Early Development ◽

Expression Profiles ◽

Critical Role ◽

Genomic Research ◽

Go Annotation ◽

Rna Molecules ◽

Non Coding Rna ◽

Mirnas Expression ◽

Paired End Sequencing

The budgerigar (Melopsittacus undulatus) is one of the most widely studied parrot species, serving as an excellent animal model for behavior and neuroscience research. Until recently, it was unknown how sexual differences in the behavior, physiology, and development of organisms are regulated by differential gene expression. MicroRNAs (miRNAs) are endogenous short non-coding RNA molecules that can post-transcriptionally regulate gene expression and play a critical role in gonadal differentiation as well as early development of animals. However, very little is known about the role gonadal miRNAs play in the early development of birds. Research on the sex-biased expression of miRNAs in avian gonads are limited, and little is known aboutM. undulatus. In the current study, we sequenced two small non-coding RNA libraries made from the gonads of adult male and female budgerigars using Illumina paired-end sequencing technology. We obtained 254 known and 141 novel miRNAs, and randomly validated five miRNAs. Of these, three miRNAs were differentially expressed miRNAs and 18 miRNAs involved in sexual differentiation as determined by functional analysis with GO annotation and KEGG pathway analysis. In conclusion, this work is the first report of sex-biased miRNAs expression in the budgerigar, and provides additional sequences to the avian miRNAome database which will foster further functional genomic research.

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