Rice callus physiology - Identification of volatile emissions and their effects on culture growth

1990 ◽  
Vol 78 (4) ◽  
pp. 526-531 ◽  
Author(s):  
S. W. Adkins ◽  
T. Shiraishi ◽  
J. A. McComb
Keyword(s):  
Rice Callus ◽  
Volatile Emissions ◽  
Culture Growth

Glutamyl and Glutaminyl-tRNA Synthetases Are a Promising Target for the Design of Threonine-Producing Strain

2019 ◽  
Vol 35 (6) ◽  
pp. 39-50
Author(s):  
T.V. Yuzbashev ◽  
A.S. Fedorov ◽  
F.V. Bondarenko ◽  
A.S. Savchenko ◽  
T.V. Vybornaya ◽  
...  
Keyword(s):  
Biomass Accumulation ◽  
Trna Synthetase ◽  
Temperature Sensitive ◽  
Original Strain ◽  
Trna Synthetases ◽  
Resource Center ◽  
Promising Target ◽  
The Russian Federation ◽  
Increase In Productivity ◽  
Culture Growth

The present work describes an approach that improves the properties of the strain producing L-threonine via the reduction in the biomass accumulation during fermentation. Glutamyl- and glutaminyl-tRNA synthetases were chosen as targets. Mutants carrying temperature-sensitive alleles were obtained. It was shown that the used system caused the suppression of the function of tRNA synthetases which led to a rapid arrest of the culture growth, and an increase in productivity and yield of the L-threonine synthesis. One of the temperature-sensitive strains was used to obtain under non-permissive conditions of mutants with the suppressed above phenotype. Some of these mutants accumulate less biomass and produce by 10-12% more threonine than the original strain. Escherichia coli, producing strain, threonine, aminoacyl-tRNA synthetase, ts-mutation This work was supported by the Ministry of Science and Higher Education of the Russian Federation (project code RFMEFI61017X0011), and it was carried out using the equipment of the National Bio-Resource Center All-Russian Collection of Industrial Microorganisms, NRC «Kurchatov Institute» - GosNIIgenetika.

Configurations of Culture Growth. A. L. Kroeber

1946 ◽  
Vol 18 (2) ◽  
pp. 163-165
Author(s):  
Max Savelle
Keyword(s):  
Culture Growth

Low moisture stress influences plant volatile emissions affecting herbivore interactions in tomato, Solanum lycopersicum

2021 ◽  
Author(s):  
Kamala Jayanthi Pagadala Damodaram ◽  
Hanamant Shivalingappa Gadad ◽  
Saravan Kumar Parepally ◽  
Sridhar Vaddi ◽  
Laxman Ramanna Hunashikatti ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Moisture Stress ◽  
Volatile Emissions ◽  
Plant Volatile ◽  
Herbivore Interactions ◽  
Stress Influences

The effect of natural and artificial periphytic substrates with biofloc system on shrimp Penaeus vannamei (Boone 1931) culture: growth and immune response

2021 ◽  
Author(s):  
Sundaram Mani ◽  
Sivakumar Mullaivanam Ramasamy ◽  
Saranya Chakrapani ◽  
Ambiganandam Krishna ◽  
Shyne Anand P.S ◽  
...  
Keyword(s):  
Immune Response ◽  
Penaeus Vannamei ◽  
Culture Growth

scholarly journals Response of Bioactive Metabolite and Biosynthesis Related Genes to Methyl Jasmonate Elicitation in Codonopsis pilosula

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 533 ◽  
Author(s):  
Jiao-jiao Ji ◽  
Qi Feng ◽  
Hai-feng Sun ◽  
Xue-jun Zhang ◽  
Xiao-xiao Li ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Adventitious Roots ◽  
Multiple Shoots ◽  
Bioactive Metabolites ◽  
Bioactive Metabolite ◽  
Codonopsis Pilosula ◽  
Meja Treatment ◽  
Metabolite Accumulation ◽  
Culture Growth ◽  
Sucrose Level

Bioactive metabolites in Codonopsis pilosula are of particular interest as an immunostimulant. Methyl jasmonate (MeJA) plays an important role in the elicitation of metabolite biosynthesis. Here, we explored the response of metabolites to MeJA elicitation in C. pilosula adventitious roots and multiple shoots. The results showed that the biomass, polysaccharide, and lobetyolin content of adventitious roots exhibited the highest increases with 100 µmol·L−1 MeJA at the 16th day of subculture, whereas the atractylenolide III (a terpenoid) content increased extremely with 50 µmol·L−1 MeJA treatment at the 7th day of subculture. In addition, the biomass and lobetyolin content significantly increased at the 4th day after treatment. Similarly, the polysaccharide and lobetyolin content increased in multiple shoots. Further identification of different metabolites responding to MeJA by 1H-NMR showed an extremely significant increase of the lobetyolinin level, which coincided with lobetyolin. Accordingly, the precursor, fatty acids, showed a highly significant decrease in their levels. Furthermore, a significant increase in β-d-fructose-butanol glycoside was detected, which was accompanied by a decrease in the sucrose level. Accordingly, the enzyme genes responsible for terpenoid and carbohydrate biosynthesis, CpUGPase, and CpPMK, were up regulated. In conclusion, MeJA promoted culture growth and accelerated bioactive metabolite accumulation by regulating the expression of the metabolite biosynthesis related genes, CpUGPase and CpPMK in C. pilosula.

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