scholarly journals Correction to: A new approach for calculating microalgae culture growth based on an inhibitory effect of the surrounding biomass

2021 ◽  
Author(s):  
Sun-Hwa Jung ◽  
Christopher McHardy ◽  
Cornelia Rauh ◽  
Alexander Jahn ◽  
Giovanni Luzi ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
New Approach ◽  
Microalgae Culture ◽  
Culture Growth

Reversal of the platelet inhibitory effect of the P2Y12 inhibitors clopidogrel, prasugrel, and ticagrelor in vitro: a new approach to an old issue

2017 ◽  
Vol 106 (11) ◽  
pp. 868-874 ◽  
Author(s):  
Lisa Schoener ◽  
Stefanie Jellinghaus ◽  
Bernhardt Richter ◽  
Christian Pfluecke ◽  
Georg Ende ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
New Approach ◽  
P2y12 Inhibitors

scholarly journals Competitive Inhibitory Effect of Calcium Polypeptides on Cd Enrichment of Brassia campestris L.

2019 ◽  
Vol 16 (22) ◽  
pp. 4472
Author(s):  
Hongbing Chen ◽  
Fangfang Shu ◽  
Sheng Yang ◽  
Yadong Li ◽  
Shilin Wang
Keyword(s):  
Competitive Inhibition ◽  
New Technology ◽  
Essential Element ◽  
Inhibitory Effect ◽  
New Approach ◽  
Mining Areas ◽  
Significant Difference ◽  
And Control ◽  
Fertilizer Effect

Most cadmium-polluted farmland and land surrounding mining areas are difficult to repair and control, seriously threatening the food safety of the crops planted in these regions. As an essential element for plant growth, calcium plays an important role in stress-resistance regulation. In this study, Brassia campestris L. was used as the experimental material and polluted soil with cadmium was used as the experimental soil sample, to explore the competition inhibition of calcium polypeptide application on the absorption of Cd2+ by Brassia campestris L. in the growth process, as well as the effect of calcium application on the growth. Results showed that the application of calcium polypeptides significantly promoted the growth of Brassia campestris L. Calcium polypeptides could be used as high-quality fertilizer, alleviating the effect of Cd2+ stress on the growth of Brassia campestris L., and promoting the absorption of K+, Ca2+, and other nutrients by Brassia campestris L. Under different calcium polypeptide application conditions, the effective state of Cd2+ in the soil showed less significant difference, indicating that the calcium polypeptide had weak or limited passivation effects on Cd2+. There was a significantly negative correlation between Cd concentration in Brassia campestris L. and calcium application (r = −0.99, p < 0.01) when calcium polypeptide was over-applied, which indicates that the inhibition effect of Cd2+ absorption on Brassia campestris L. is mainly through competitive inhibition rather than passivation. The results showed that calcium polypeptide has dual functions on the competitive inhibition of heavy metals and a good fertilizer effect, providing a new technology for in situ remediation of heavy-metal pollution, and a new approach for the treatment of cadmium-contaminated farmland and surrounding mining land.

scholarly journals The Effect of Kanamycin and Tetracycline on Growth and Photosynthetic Activity of Two Chlorophyte Algae

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Khawaja Muhammad Imran Bashir ◽  
Man-Gi Cho
Keyword(s):  
Photosynthetic Activity ◽  
Protein A ◽  
Inhibitory Effect ◽  
Cell Protein ◽  
Algal Culture ◽  
Slight Reduction ◽  
Minor Effect ◽  
Photosynthetic Inhibition ◽  
Microalgae Culture ◽  
Culture Maintenance

Antibiotics are routinely used in microalgae culture screening, stock culture maintenance, and genetic transformation. By studying the effect of antibiotics on microalgae growth, we can estimate the least value to inhibit growth of undesired pathogens in algal culture. We studied the effect of kanamycin and tetracycline on the growth and photosynthetic activity of two chlorophyte microalgae,Dictyosphaerium pulchellumandMicractinium pusillum.We measured CFU mL−1on agar plates, optical density, fluorescence yields, and photosynthetic inhibition. Our results showed a significant effect ofkanandteton the tested microalgae species excepttet,which showed a minor effect onM. pusillum.Both antibiotics are believed to interact with the protein synthesis machinery; hence, the inhibitory effect of the tested antibiotics was further confirmed by isolation and quantification of the whole cell protein. A significant reduction in protein quantity was observed at concentrations more than 5 mg L−1, exceptM. pusillum, which showed only a slight reduction in protein quantity even at the maximum tested concentration oftet(30 mg L−1). This study can further aid in aquaculture industry, for the maintenance of the microalgae stock cultures and it can also help the microalgae genetic engineers in the construction of molecular markers.

Conversion of renin substrate tetradecapeptide to angiotensin II by rat MAB elastase-2

2004 ◽  
Vol 82 (11) ◽  
pp. 1000-1005 ◽  
Author(s):  
Carlos F Santos ◽  
Andrew S Greene ◽  
Maria Cristina O Salgado ◽  
Eduardo B Oliveira
Keyword(s):  
Angiotensin Ii ◽  
Enzymatic Activity ◽  
Catalytic Efficiency ◽  
Inhibitory Effect ◽  
Ang Ii ◽  
Chromogenic Substrates ◽  
Renin Substrate ◽  
New Approach ◽  
Pancreatic Elastase ◽  
Mesenteric Arterial Bed

A new approach for the purification of rat mesenteric arterial bed (MAB) elastase-2 has been developed using the chromogenic substrates N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide and N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide to monitor the enzymatic activity during various stages of purification. The purified enzyme was evaluated in the presence of various inhibitors and confirmed to have angiotensin (Ang) II-forming ability. The active site-directed inhibitor acetyl-Ala-Ala-Pro-Leu-chloromethylketone (100 µmol·L-1), described for human pancreatic elastase-2, abolished the enzymatic activity, confirming that the enzyme is an elastase-2. Chymostatin (100 µmol·L-1), an inhibitor regarded as selective for chymases, also showed a remarkable inhibitory effect (94%), whereas captopril (100 µmol·L-1) had no effect at all on the Ang II-forming activity. The Ang II precursor renin substrate tetradecapeptide (RS-14P) was converted into Ang II by the rat MAB elastase-2 with the following kinetic constants: Km = 124 ± 21 µmol·L-1; Kcat = 629 min-1; catalytic efficiency (Kcat /Km) = 5.1 min-1 µ(mol/L)-1. In conclusion, the strategy for the purification of rat MAB elastase-2 with the chromogenic substrates proved to be simple, rapid, accurate, and highly reproducible; therefore, it can be reliably and conveniently used to routinely purify this enzyme. The kinetic parameters for the formation of Ang II from RS-14P by rat MAB elastase-2 emphasize differences in substrate specificity between this and other Ang II-forming enzymes.Key words: N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide, elastase-2, angiotensin II, renin substrate tetradecapeptide.

scholarly journals Temperature dependence of allelopathy duality and its influence on boreal forest succession-A case analysis of Picea schrenkiana

2019 ◽  
Author(s):  
Xiao Ruan ◽  
Li Yang ◽  
Min-fen Yu ◽  
Zhao-hui Li ◽  
Ying-xian Zhao ◽  
...  
Keyword(s):  
Global Warming ◽  
Antioxidant Enzyme ◽  
Seedling Growth ◽  
Inhibitory Effect ◽  
Experimental Simulation ◽  
Dark Light ◽  
New Approach ◽  
New Findings ◽  
Intrinsic Mechanism ◽  
The Relationship

AbstractGlobal warming in conjunction with various biotic or abiotic interferences has been jeopardizing the ecosystem of boreal forests. By integrating field inspection with experimental simulation, this work comprehensively investigated the allelopathic effects of a key allelochemical 3,4-dihydroxyacetophenone (DHAP) in the exudates of P. schrenkiana needles on its seed and seedling growth, endogenous hormone metabolism and antioxidant enzyme activity, identified the existence of DHAP allelopathy duality at a certain temperature with an inflection concentration point (e.g. about 0.25 mM at dark/light temperature of 4/12 °C) as the boundary between promotional and inhibitory effect, and verified that the inflection point of DHAP concentration would inevitably shift to a lower level as temperature increased. Consequently, this paper gives a scientific explanation into the intrinsic mechanism of P. schrenkiana degradation due to allelopathy, but also presents a new approach to explore the relationship between forest evolution and global warming.HighlightA quantitative description on the duality of 3, 4-dihydroxyacetophenone (DHAP) as a promoter or an inhibitor to affect the seed germination, seedling growth and root development of P. schrenkiana, as well as the antioxidant enzyme activities and hormone contents.The new findings of DHAP inflection concentration as boundary to divide the promotional and inhibitory effect of allelopathy which would decrease as environment temperatures rise.An explanation into the intrinsic mechanism of P. schrenkiana degradation due to allelopathy, and a new approach to explore the relationship between forest evolution and global warming.

scholarly journals Anti-metastatic Activities of Bibenzyls from Dendrobium pulchellum

2013 ◽  
Vol 8 (1) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Pithi Chanvorachote ◽  
Akkarawut Kowitdamrong ◽  
Thidarat Ruanghirun ◽  
Boonchoo Sritularak ◽  
Chutichot Mungmee ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Drug Development ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Preliminary Data ◽  
Inhibitory Effect ◽  
Cancer Drug ◽  
New Approach ◽  
Cell Metastasis ◽  
First Time

Our investigation of the stem of Dendrobium pulchellum resulted in the isolation of four known bibenzyls, chrysotobibenzyl (1), chrysotoxine (2), crepidatin (3) and moscatilin (4). The present study reveals for the first time the ability of these four compounds to facilitate anoikis and inhibit the growth of lung cancer cells in anchorage-independent condition. The preliminary data obtained disclose the inhibitory effect on cancer cell metastasis of the isolated compounds, and provide an important new approach for cancer drug development.

scholarly journals Antagonistic Activity of Fungal Endophytes Isolated from Garcinia atroviridis against Colletotrichum gloeosporioides

2020 ◽  
Vol 27 (3) ◽  
pp. 209
Author(s):  
Nur Afeeqah Binti Mohamed Zanudin ◽  
Nor'Aishah Hasan ◽  
Patahayah Binti Mansor
Keyword(s):  
Endophytic Fungi ◽  
Plant Disease ◽  
Colletotrichum Gloeosporioides ◽  
Fungal Endophytes ◽  
Inhibitory Effect ◽  
Antagonistic Activity ◽  
Dual Culture ◽  
Antagonistic Microorganisms ◽  
Culture Growth ◽  
Biocontrol Potential

The extensive use of synthetic fungicides in controlling plant disease generates detrimental impacts on the environment and human health. In response to this problem, an alternative method was developed, known as biological control using antagonistic microorganisms. Since investigation on fungal endophytes of Garcinia atroviridis is still unclear, it was chosen for the study. The aim of the present work was to evaluate biocontrol potential of endophytic fungi against Colletotrichum gloeosporiodes, a phytopathogen that caused anthracnose disease. A total of 92 endophytic fungi were isolated from different tissue parts of Garcinia atroviridis including leaves, petioles, branches, and fruits. Results demonstrated that, most of endophytic fungal isolates showed some inhibitory action over the growth of C. gloeosporiodes during dual culture growth. Endophyte isolate F14 showed the highest antagonistic activity against Colletotrichum gloeosporiodes with 67.38% percentage inhibition radial growth (PIRG). However, 7 out of 92 isolates showed no inhibitory effect against Colletotrichum gloeosporiodes. In conclusion, endophytic fungi isolated from G. atroviridis indicate the potential as biocontrol agents. It is hoped that the finding of isolated endophytic fungi in this study with antagonistic activity against anthracnose pathogen may be used in biocontrol programmes of plant disease in the region.

scholarly journals A Strategy Based on GC-MS/MS, UPLC-MS/MS and Virtual Molecular Docking for Analysis and Prediction of Bioactive Compounds in Eucalyptus Globulus Leaves

2019 ◽  
Vol 20 (16) ◽  
pp. 3875 ◽  
Author(s):  
Pan ◽  
Lei ◽  
Zang ◽  
Zhang
Keyword(s):  
Molecular Docking ◽  
Medicinal Plants ◽  
Gallic Acid ◽  
Inhibitory Activity ◽  
Eucalyptus Globulus ◽  
Specific Activity ◽  
Inhibitory Effect ◽  
Scoring Functions ◽  
Active Ingredients ◽  
New Approach

The discovery of medicinal plants is crucial for drug development. Eucalyptus globulus leaves are used as a traditional medicine in many areas of world due to herbicidal and insecticidal activity. While natural products are difficult to be separated and activity assayed, a new approach is needed to predict the active ingredients therein. In this study, a new method for screening active compounds extracted from E. globulus leaves was developed by GC-MS/MS and UPLC-MS/MS combined with molecular docking technology. Predicted compounds with high activity were proposed. Firstly, 35 volatile compounds and 34 aqueous extracted compounds were extracted from E. globulus leaves, and identified by GC-MS/MS and UPLC-MS/MS. The herbicidal receptor (1BX9) was then docked with the identified compounds by docking software, evaluated by docking models and seven scoring functions. The results showed that gallic acid had a strong inhibitory activity of 1BX9, which was speculated to be the main reason for the inhibitory effect of E. globulus leaves. Finally, allelopathic tests of gallic acid, citric acid, and isopulegol were carried out on grass seeds to verify its inhibitory activity against herbicide receptor 1BX9. The results show that the method can screen compounds with specific activity from a complex system of medicinal plants, which is very important for the screening of new active ingredients, confirmation of new medicinal ingredients, and the in-depth development of animal and plant medicines.

scholarly journals Two-Component Model of Microalgae Growth in the Turbidostat

2021 ◽  
Vol 16 (1) ◽  
pp. 101-114
Author(s):  
A.S. Lelekov ◽  
R.P. Trenkenshu
Keyword(s):  
Plastic Substrate ◽  
Limiting Factor ◽  
Microalgae Culture ◽  
Cell Biomass ◽  
Cell Structures ◽  
Physicochemical Factors ◽  
Two Component ◽  
Culture Growth ◽  
Light Energy Conversion ◽  
Component Models

The paper focuses on the study of light influence mechanisms on microalgae culture growth in the turbidostat. The method of turbidostat culture provides the same light conditions for all cells, stabilization of their biochemical composition, as well as the constancy of all physicochemical factors of the environment. The main approaches and principles of modeling the microalgae culture growth are presented. Modern models are shown to be based on classical concepts of considering cell biomass as the sum of two or more compounds. The use of two-component models for microalgae is due to both photochemical and enzymatic processes of biosynthesis of cell structures from mineral substances due to the energy of high-potential forms of macroergs. The proposed mathematical model is represented by a system of two differential equations describing the synthesis of reserve biomass compounds at the expense of light and biosynthesis of structural components from reserve ones. The model takes into account that a part of the reserve compounds is spent on replenishing the pool of macroergs, and a part of the structural component can be converted into a reserve one. The rates of synthesis of structural and reserve forms of biomass are given by linear splines and expressed in terms of the reduced fluxes of the energy or plastic substrate. The model was verified on the experimental data of the chlorophyllostat culture Tetraselmis viridis. It is shown that the light curve of T. viridis is characterized by a double change in the limiting factor and can be divided into a region of metabolic, light limiting and a region of saturation. A decrease in the specific growth rate is related to a decrease in the efficiency of light energy conversion.

Sign in / Sign up

Export Citation Format

Share Document