scholarly journals Interstitial Fluid Adenosine and Sagittal Sinus Blood Flow during Bicuculline-Seizures in Newborn Piglets

1987 ◽  
Vol 7 (5) ◽  
pp. 633-639 ◽  
Author(s):  
T. S. Park ◽  
David G. L. Van Wylen ◽  
Rafael Rubio ◽  
Robert M. Berne
Keyword(s):  
Blood Flow ◽  
Interstitial Fluid ◽  
Cerebrovascular Resistance ◽  
Newborn Piglets ◽  
Sagittal Sinus ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
The Brain ◽  
Level Group

Changes of interstitial fluid adenosine concentrations and effects of O2 supply on interstitial fluid adenosine were studied by the brain dialysis technique in the frontal cortex of newborn piglets subjected to bicuculline-induced seizures. The O2 supply was changed globally by changing MABP and locally by varying PO2 in the artificial CSF perfusing the dialysis cannula. Sagittal sinus blood flow (SSBF), cerebrovascular resistance (CVR), and CMRO2 were also examined in the same animals. Seizures increased interstitial fluid adenosine 7.9-fold (p < 0.05) when ictal MABP was maintained at preictal level and perfusate PO2 was 24 mm Hg (group 1, n = 6). Interstitial fluid adenosine increased 11.8-fold (p < 0.05) during seizures associated with moderate systemic hypotension and the low perfusate PO2 (group 2, n = 6). By contrast, seizures increased interstitial fluid adenosine three-fold (p < 0. 05) when perfusate PO2 was increased to 182 mm Hg and ictal MABP was maintained at preictal level (group 3, n = 8). When ictal MABP was elevated from the preictal level and the perfusate was rich in oxygen, seizures failed to increase interstitial fluid adenosine (group 4, n = 7). In groups 1 and 3, the increase in interstitial fluid adenosine during seizures was associated with significant increases in SSBF and CMRO2, as well as significant decreases in CVR. These data suggest that the increase in O2 supply during seizures in piglets did not match completely the increase in O2 demand and resulted in enhanced release of adenosine into the interstitial space.

scholarly journals Brain Interstitial Adenosine and Sagittal Sinus Blood Flow during Systemic Hypotension in Piglet

1988 ◽  
Vol 8 (6) ◽  
pp. 822-828 ◽  
Author(s):  
T. S. Park ◽  
David G. L. Van Wylen ◽  
Rafael Rubio ◽  
Robert M. Berne
Keyword(s):  
Blood Flow ◽  
Interstitial Fluid ◽  
Cerebrovascular Resistance ◽  
Sagittal Sinus ◽  
Group 4 ◽  
Brain Dialysis ◽  
Group 3 ◽  
Group 2 ◽  
The Brain ◽  
Dialysis Technique

We sampled, using the brain dialysis technique, interstitial fluid adenosine from the frontal cortex of newborn piglets subjected to hemorrhagic hypotension while measuring sagittal sinus blood flow, cerebrovascular resistance (CVR), and cerebral O2 delivery. In group 1 (n = 8), MABP was reduced in successive steps from 76 to 30 mm Hg with decrements of ∼ 10 mm Hg. At 60 mm Hg, CVR decreased by 19% (p < 0.001), but sagittal sinus blood flow and interstitial fluid adenosine remained unchanged. At 50 mm Hg, both sagittal sinus blood flow and CVR decreased by 19% (p < 0.001) and interstitial fluid adenosine rose 4.7-fold (p < 0.05). At 40 and 30 mm Hg, sagittal sinus blood flow decreased further but CVR remained steady, whereas interstitial fluid adenosine rose 10- and 16-fold, respectively. In group 2 (n = 7), an abrupt reduction of MABP from 80 to 47 mm Hg produced no change in sagittal sinus blood flow and a 29% decrease in CVR (p < 0.01). Interstitial fluid adenosine increased twofold (p < 0.01). In group 3 (n = 7), an abrupt reduction of MABP from 79 to 40 mm Hg decreased sagittal sinus blood flow and CVR by 24 and 30%, respectively (p < 0.01). Interstitial fluid adenosine rose threefold (p < 0.01). In groups 1, 2, and 3, the increases in interstitial fluid adenosine accompanied decreases in cerebral O2 delivery. In group 4 (n = 7), artificial CSF with a Po2 of 152 mm Hg was perfused through the brain dialysis cannula during graded hypotension. In this group, interstitial fluid adenosine rose only at an MABP of 20 mm Hg. These data support the concept that adenosine participates in the regulation of CBF during hypotension in piglets.

Correlation of γ-radioactivity and Scanning Electron Microscopy in measurement of retention of 111Indium-labeled canine endothelial cells on synthetic vascular grafts

1989 ◽  
Vol 47 ◽  
pp. 886-887
Author(s):  
E.J. Prendiville ◽  
S. Laliberté Verdon ◽  
K. E. Gould ◽  
K. Ramberg ◽  
R. J. Connolly ◽  
...  
Keyword(s):  
Blood Flow ◽  
Ex Vivo ◽  
Vascular Grafts ◽  
Retention Data ◽  
Vascular Prostheses ◽  
Group 4 ◽  
Human Fibronectin ◽  
Insufficient Time ◽  
Group 3 ◽  
Group 2

Endothelial cell (EC) seeding is postulated as a mechanism of improving patency in small caliber vascular grafts. However the majority of seeded EC are lost within 24 hours of restoration of blood flow in previous canine studies . We postulate that the cells have insufficient time to fully develop their attachment to the graft surface prior to exposure to hemodynamic stress. We allowed EC to incubate on fibronectin-coated ePTFE grafts for four different time periods after seeding and measured EC retention after perfusion in a canine ex vivo shunt circuit.Autologous canine EC, were enzymatically harvested, grown to confluence, and labeled with 30 μCi 111 Indium-oxine/80 cm 2 flask. Four groups of 5 cm x 4 mm ID ePTFE vascular prostheses were coated with 1.5 μg/cm.2 human fibronectin, and seeded with 1.5 x 105 EC/ cm.2. After seeding grafts in Group 1 were incubated in complete growth medium for 90 minutes, Group 2 were incubated for 24 hours, Group 3 for 72 hours and Group 4 for 6 days. Grafts were then placed in the canine ex vivo circuit, constructed between femoral artery and vein, and subjected to blood flow of 75 ml per minute for 6 hours. Continuous counting of γ-activity was made possible by placing the seeded graft inside the γ-counter detection crystal for the duration of perfusion. EC retention data after 30 minutes, 2 hours and 6 hours of flow are shown in the table.

Influence of adenosine on cerebral blood flow during hypoxic hypoxia in the newborn piglet

1990 ◽  
Vol 68 (4) ◽  
pp. 1534-1541 ◽  
Author(s):  
N. Laudignon ◽  
E. Farri ◽  
K. Beharry ◽  
J. Rex ◽  
J. V. Aranda
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
High Performance ◽  
Severe Hypoxia ◽  
Group 4 ◽  
Csf Levels ◽  
Group 3 ◽  
Group 2 ◽  
Arterial Po2 ◽  
Group 1

This study investigated the role of adenosine in the regulation of neonatal cerebral blood flow (CBF) during moderate (arterial PO2 = 47 +/- 9 Torr) and severe (arterial PO2 = 25 +/- 4 Torr) hypoxia. Twenty-eight anesthetized and ventilated newborn piglets were assigned to four groups: 8 were injected intravenously with the vehicle (controls, group 1); 13 received an intravenous injection of 8-phenyltheophylline (8-PT), a potent adenosine receptor blocker, either 4 mg/kg (group 2, n = 6, mean cerebrospinal fluid (CSF) levels less than 1 mg/l) or 8 mg/kg (group 3, n = 7, mean CSF levels less than 3.5 mg/l); and 7 received an intracerebroventricular injection of 10 micrograms 8-PT (group 4). During normoxia, CBF was not altered by vehicle or 8-PT injections. In group 1, 10 min of moderate and severe hypoxia increased total CBF by 112 +/- 36 and 176 +/- 28% (SE), respectively. Compared with controls, the cerebral hyperemia during moderate hypoxia was not altered in group 2, attenuated in group 3 (to 53 +/- 13%, P = NS), and completely blocked in group 4 (P less than 0.01). CBF increase secondary to severe hypoxia was attenuated only in group 4 (74 +/- 29%, P less than 0.05). CSF concentrations of adenosine and adenosine metabolites measured by high-performance liquid chromatography increased during hypoxia. Arterial O2 content was inversely correlated (P less than 0.005) to maximal CSF levels of adenosine (r = 0.73), inosine (r = 0.87), and hypoxanthine (r = 0.80).(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals The Level of Reactive Carbonyl Derivatives of Proteins, Malondialdehyde, and Catalase Activity in the Brain of Rats after Therapy Following Chronic Unpredictable Moderate Stress

2020 ◽  
Vol 8 (A) ◽  
pp. 691-698
Author(s):  
Yelena Valerievna Yepifantseva ◽  
Mayra Galimzhanovna Abdrakhmanova ◽  
Yelena Vladimirovna Pozdnyakova ◽  
Polina Sergeyevna Semenikhina ◽  
Ruslan Andreevich Belyayev ◽  
...  
Keyword(s):  
Behavioral Disorders ◽  
Oxidative Metabolism ◽  
Protein Carbonylation ◽  
Carbonyl Derivatives ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Derivatives Of ◽  
The Brain ◽  
Group 1

BACKGROUND: Understanding the mechanisms of the behavioral disorders’ emergence under the influence of chronic stress is the most important aspect of the subsequent development of a strategy for its therapy and prevention. Changes in the oxidative metabolism processes can be decisive in the development of the pathogenetic cascade in the brain. Information about these processes can be obtained by studying protein carbonylation, lipid peroxidation, and catalase activity (CA). The complexity of the therapeutic impact in various behavioral disorders implies the search for new pharmacological substances and the study of the previously known drugs’ effects based on the available scientific data. AIM: The aim of the study was to study the reactive carbonyl derivatives of proteins (RCDP), malondialdehyde (MDA), and CA in the brain of rats after therapy following chronic unpredictable moderate stress (CUMS). METHODS: Forty male outbred rats weighing 450–500 g were used in this study. For 21 days, all animals were exposed to the diverse stress factors for developing the CUMS. The animals were divided into four groups of 10 rats, each using randomized selection. The rats of one group were euthanized by decapitation with subsequent brain harvesting (Group 4). Remaining three groups of rats were treated with placebo (Group 1), harmine hydrochloride (Group 2), and amitriptyline (Group 3) for 21 days. Upon completion of therapy, all rats were also euthanized by decapitation with subsequent brain harvesting. The levels of RCDP, MDA, and CA were studied in their brain, and after that, we compared the multiple studied indicators in four groups. RESULTS: The results of the rat brain examinations in four groups showed that RCDP level in Group 2 was significantly lower than in Group 4 (p = 0.000). Similarly, in Group 1, it was lower than in Group 4 (p = 0.021), plus, it did not differ statistically from the harmine hydrochloride group (p = 1,000). Indicators of Groups 3 and 4 did not have any significant differences in RCDP level, too, (p = 0.799); however, the RCDP level in Group 2 was significantly lower than in Group 3 (p = 0.040). MDA indicators did not show significant differences; however, a tendency for lower values was revealed in Group 1 (p = 0.233) and Group 2 (p = 0.151). CA in Group 4 was lower than that in Group 1 (p = 0.000), Group 2 (p = 0.001), and Group 3 (p = 0.003) contemporaneously, while all treatment groups were comparable (p = 1.000). CONCLUSION: The result of exposure to chronic stress can be reproduced with the best quality in the CUMS model. The neurobiological foundations of the model make it possible to assess biochemical markers of oxidative metabolism and evaluate the possibilities of pharmacological correction of stress-induced behavioral disorders. To assess the mechanisms of autoregulation of oxidative metabolism, this study included a placebo group (Group 1), the level of RCDP in which was significantly higher in comparison with Group 3 and Group 4 and slightly lower than in Group 2. In this study, harmine hydrochloride demonstrated activity exceeding amitriptyline, particularly limiting the process of protein carbonylation, not noted for amitriptyline. According to the results of the RCDP assessment in the CUMS model, the process of protein carbonylation can be considered to be one of the significant factors in the deactivation of neurotransmitters. The CA levels determined in all groups allowed us to consider this marker as the most sensitive to the effects of stress, which possibly has an inhibitory effect on catalase, as its activity in all groups after therapy was more than two-fold higher than in animals right after CUMS. We can assume that CA plays an important role in starting the processes of autoregulation of oxidative metabolism. The study was carried out as a part of the implementation of the scientific and technical program No. BR05236584 “Development of new herbal preparations and their pharmacological and clinical studies” (O.0820). (2018–2020) in the priority area, “Life and Health Sciences.”

scholarly journals Circulatory Rejuvenated EPCs Derived from PAOD Patients Treated by CD34+ Cells and Hyperbaric Oxygen Therapy Salvaged the Nude Mouse Limb against Critical Ischemia

2020 ◽  
Vol 21 (21) ◽  
pp. 7887
Author(s):  
Yin-Chia Chen ◽  
Jiunn-Jye Sheu ◽  
John Y. Chiang ◽  
Pei-Lin Shao ◽  
Shun-Cheng Wu ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cytochrome C ◽  
Nude Mouse ◽  
Hyperbaric Oxygen ◽  
Nude Mice ◽  
Group 4 ◽  
Protein Expressions ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

This study tested whether circulatory endothelial progenitor cells (EPCs) derived from peripheral arterial occlusive disease (PAOD) patients after receiving combined autologous CD34+ cell and hyperbaric oxygen (HBO) therapy (defined as rejuvenated EPCs) would salvage nude mouse limbs against critical limb ischemia (CLI). Adult-male nude mice (n = 40) were equally categorized into group 1 (sham-operated control), group 2 (CLI), group 3 (CLI-EPCs (6 × 105) derived from PAOD patient’s circulatory blood prior to CD34+ cell and HBO treatment (EPCPr-T) by intramuscular injection at 3 h after CLI induction) and group 4 (CLI-EPCs (6 × 105) derived from PAOD patient’s circulatory blood after CD34+ cell and HBO treatment (EPCAf-T) by the identical injection method). By 2, 7 and 14 days after the CLI procedure, the ischemic to normal blood flow (INBF) ratio was highest in group 1, lowest in group 2 and significantly lower in group 4 than in group 3 (p < 0.0001). The protein levels of endothelial functional integrity (CD31/von Willebrand factor (vWF)/endothelial nitric-oxide synthase (eNOS)) expressed a similar pattern to that of INBF. In contrast, apoptotic/mitochondrial-damaged (mitochondrial-Bax/caspase-3/PARP/cytosolic-cytochrome-C) biomarkers and fibrosis (Smad3/TGF-ß) exhibited an opposite pattern, whereas the protein expressions of anti-fibrosis (Smad1/5 and BMP-2) and mitochondrial integrity (mitochondrial-cytochrome-C) showed an identical pattern of INBF (all p < 0.0001). The protein expressions of angiogenesis biomarkers (VEGF/SDF-1α/HIF-1α) were progressively increased from groups 1 to 3 (all p < 0.0010). The number of small vessels and endothelial cell surface markers (CD31+/vWF+) in the CLI area displayed an identical pattern of INBF (all p < 0.0001). CLI automatic amputation was higher in group 2 than in other groups (all p < 0.001). In conclusion, EPCs from HBO-C34+ cell therapy significantly restored the blood flow and salvaged the CLI in nude mice.

scholarly journals Strength Training Habits in Amateur Endurance Runners in Spain: Influence of Athletic Level

2020 ◽  
Vol 17 (21) ◽  
pp. 8184
Author(s):  
Felipe García-Pinillos ◽  
Carlos Lago-Fuentes ◽  
Diego Jaén-Carrillo ◽  
Pascual Bujalance-Moreno ◽  
Pedro Ángel Latorre-Román ◽  
...  
Keyword(s):  
Strength Training ◽  
Plyometric Training ◽  
Online Questionnaire ◽  
Group 4 ◽  
Group 3 ◽  
Endurance Runners ◽  
Group 5 ◽  
Training Habits ◽  
Group 2 ◽  
Level Group

This study determined the strength training (ST) habits of amateur endurance runners in Spain regarding athletic level. A sixteen-item online questionnaire comprised of (i) demographic information, (ii) performance, and (iii) training contents was completed by 1179 athletes. Five group levels were determined according to the personal best times of the athletes in a 10-km trial (LG1: level group 1, 50–55 min; LG2: level group 2, 45–50 min; LG3: level group 3, 40–45 min; LG4: level group 4, 35–40 min; LG5: level group 5, 30–35 min). Most athletes (n = 735, 62.3%) perceived ST as being a key component in their training program. Resistance training (RT) was reported as a ST type used by 63.4% of the athletes, 66.9% reported using bodyweight exercises, 46.8% reported using plyometric training, 65.6% reported using uphill runs, and 17.8% reported using resisted runs. The prevalence of runners who excluded ST from their training programs decreased as the athletic performance level increased (18.2% in lower-level athletes vs. 3.0% in higher-level), while the inclusion of RT, bodyweight exercises, plyometric training, and uphill and resisted runs was more frequent within higher-level groups. Most athletes included ST using low-to-moderate loads and high a number of repetitions/sets comprised of RT, plyometric training, resisted runs, and core, respiratory, and foot muscles training.

Cytarabine and Ferric Carboxymaltose (Fe+3) Increase Oxidative Damage and Alter Serotonergic Metabolism in Brain

2019 ◽  
Vol 18 (2) ◽  
pp. 149-155
Author(s):  
David Calderón Guzmán ◽  
Norma Osnaya Brizuela ◽  
Maribel Ortíz Herrera ◽  
Hugo Juárez Olguín ◽  
Armando Valenzuela Peraza ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Wistar Rats ◽  
Ferric Carboxymaltose ◽  
Anticancer Compounds ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
The Brain ◽  
Group 1

Background & Objective: The purpose of this study was to measure the effect on brain biomarkers after treatment with anticancer compounds - cytarabine (CT) and ferric carboxymaltose (FC) (Fe+3) in Wistar rats. Methods: The Wistar rats were treated as follows: group 1 (control), NaCl 0.9%; group 2, CT (25 mg/k), group 3, FC(Fe+3) (50 mg/k) and group 4, CT + FC(Fe+3). The animals were sacrificed and their brains were obtained and used to measure lipoperoxidation (TBARS), H2O2, Na+, K+ ATPase, glutathione (GSH), serotonin metabolite (5-HIAA) and dopamine. The results indicated an enhancement of lipid peroxidation in the cortex and striatum of groups treated with FC(Fe+3) and CT, while GSH decreased in the cortex of group treated with CT + FC(Fe+3). Dopamine decreased in the cortex of the rats that received CT, while in the striatum, 5HIAA increased in all groups. </P><P> Results & Conclusion: These results suggest that the treatment with CT and FC(Fe+3) boosted oxidative stress and led to an alteration in momoamine concentrations in the brain.

scholarly journals Scintigraphic study of the splenic circulation in rats

2014 ◽  
Vol 41 (5) ◽  
pp. 345-350
Author(s):  
Fábio Gontijo Rodrigues ◽  
Andy Petroianu ◽  
Simone Odília Fernandes Diniz ◽  
Valbert Nascimento Cardoso ◽  
Eduardo Vilhena de Parreira
Keyword(s):  
Blood Flow ◽  
Venous Drainage ◽  
Group 4 ◽  
Abdominal Organs ◽  
Splenic Parenchyma ◽  
Flow Through ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Iliac Bifurcation

Objective: To evaluate the splenic parenchymal blood distribution through scintigraphic study.Methods: Thirty Wistar rats were randomly divided into six groups (n = 5). Group 1 (spleen - 30 minutes) and Group 2 (spleen - 90 minutes) underwent laparotomy with direction of blood flow to the spleen by ligature of the aorta near the iliac bifurcation and splanchnic vessels, keeping blood flow only in the splenic artery; Group 3 (spleen and stomach - 30 minutes) and Group 4 (spleen and stomach - 90 minutes) underwent laparotomy with direction of blood flow to the spleen and stomach by ligature of the aorta near the iliac bifurcation and splanchnic vessels, maintaining the flow through the splenic, gastric and splenogastric vessels; Group 5 (control - 30 minutes) and Group 6 (control - 90 minutes) underwent laparotomy and ligation of the aorta near the iliac bifurcation, keeping the flow to the abdominal organs. After arterial ligation, the animals received an injection of 0.2 ml of sodium pertechnetate in the aorta. Scintigraphic images were taken and the animals had their spleens removed for radioactivity counting with an automatic counter device.Results: There was no difference in the amounts of radiation from the spleen between groups, indicating retention of the radioisotope by the spleen, even after the period of 90 minutes.Conclusion: The blood flow through the spleen is not continuous. The blood diffuses through the splenic parenchyma and its venous drainage is slow, not following a predictable sequence.

Analysis of radiosurgical results in patients with brain metastases according to the number of brain lesions: is stereotactic radiosurgery effective for multiple brain metastases?

2010 ◽  
Vol 113 (Special_Supplement) ◽  
pp. 73-78 ◽  
Author(s):  
Won Seok Chang ◽  
Hae Yu Kim ◽  
Jin Woo Chang ◽  
Yong Gou Park ◽  
Jong Hee Chang
Keyword(s):  
Brain Metastases ◽  
Brain Lesions ◽  
Rank Test ◽  
Survival Times ◽  
Group 4 ◽  
Metastatic Lesions ◽  
Log Rank Test ◽  
Group 3 ◽  
Group 2 ◽  
The Brain

Object Whole-brain radiation therapy (WBRT), open resection, and stereotactic radiosurgery (SRS) are widely used for treatment of metastatic brain lesions, and many physicians recommend WBRT for multiple brain metastases. However, WBRT can be performed only once per patient, with rare exceptions. Some patients may require SRS for multiple metastatic brain lesions, particularly those patients harboring more than 10 lesions. In this paper, treatment results of SRS for brain metastasis were analyzed, and an attempt was made to determine whether SRS is effective, even in cases involving multiple metastatic brain lesions. Methods The authors evaluated the cases of 323 patients who underwent SRS between October 2005 and October 2008 for the treatment of metastatic brain lesions. Treatment was performed using the Gamma Knife model C or Perfexion. The patients were divided into 4 groups according to the number of lesions visible on MR images: Group 1, 1–5 lesions; Group 2, 6–10 lesions: Group 3, 11–15 lesions; and Group 4, > 15 lesions. Patient survival and progression-free survival times, taking into account both local and distant tumor recurrences, were analyzed. Results The patients consisted of 172 men and 151 women with a mean age at SRS of 59 years (range 30–89 years). The overall median survival time after SRS was 10 months (range 8.7–11.4 months). The median survival time of each group was as follows: Group 1, 10 months; Group 2, 10 months; Group 3, 13 months; and Group 4, 8 months. There was no statistical difference between survival times after SRS (log-rank test, p = 0.554), although the probability of development of new lesions in the brain was greater in Group 4 (p = 0.014). Local tumor control rates were not statistically different among the groups (log-rank test, p = 0.989); however, remote disease progression was more frequent in Group 4 (log-rank test, p = 0.014). Conclusions In this study, patients harboring more than 15 metastatic brain lesions were found to have faster development of new lesions in the brain. This may be due to the biological properties of the patients' primary lesions, for example, having a greater tendency to disseminate hematogenously, especially to the brain, or a higher probability of missed or invisible lesions (microscopic metastases) to treat on stereotactic MR images at the time of radiosurgery. However, the mean survival times after SRS were not statistically different between groups. According to the aforementioned results, SRS may be a good treatment option for local control of metastatic lesions and for improved survival in patients with multiple metastatic brain lesions, even those patients who harbor more than 15 metastatic brain lesions, who, after SRS, may have early and easily detectable new metastatic lesions.

scholarly journals METHODS TO DETERMINE COMPLEX DEGREES OF MUTUAL ANISOTROPY FOR THE DIFFERENTIATION OF HEMORRHAGES IN THE HUMAN BRAIN SUBSTANCE RESULTING FROM TRAUMATIC AND NON-TRAUMATIC GENESIS

2020 ◽  
Vol 1 ◽  
pp. 18-23
Author(s):  
Marta Garazdiuk ◽  
Viktor Bachynskіy ◽  
Olena Nechytailo ◽  
Oleksandr Garazdiuk ◽  
Svitlana Malanchuk
Keyword(s):  
Cerebral Infarction ◽  
Human Brain ◽  
Group 4 ◽  
Brain Substance ◽  
Module Size ◽  
Traumatic Origin ◽  
Group 3 ◽  
Group 2 ◽  
Correlation Microscopy ◽  
The Brain

An issue that is often debated in forensic traumatology is the differential diagnosis of hemorrhages into the human brain substance (HBS) of traumatic and non-traumatic genesis. Objectives. This study aims to identify new criteria for objective forensic differentiation of hemorrhages of traumatic origin, cerebral infarction of ischemic and hemorrhagic genesis by using the method of complex degree of mutual anisotropy. For this study native sections of HBS from 125 corpses were used in the case of: death from coronary heart disease - 35 (28%) of native sections (Group 1 - control); hemorrhages of traumatic genesis - 30 (24%) sections (Group 2); ischemic cerebral infarction - 30 (24%) native sections(Group 3); and hemorrhages of non-traumatic genesis - 30 (24%) native sections (Group 4). Results. The statistical moments of the third and fourth orders, which characterize the asymmetry and excess of the complex degree of mutual anisotropy module size distributions, the strength of the method of polarization-correlation microscopy in the differentiation of the samples of the histological sections of the brain of control and experimental groups reached a good level — 87%-90%. Conclusion. The method of complex degree of mutual anisotropy allows differentiating with great precision the genesis of hemorrhage into the substance of the brain.

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