scholarly journals Autoradiographic Study of Regional Protein Synthesis in Focal Cerebral Ischemia with TCA Wash and Image Subtraction Techniques

1987 ◽  
Vol 7 (4) ◽  
pp. 387-393 ◽  
Author(s):  
T. Yoshimine ◽  
T. Hayakawa ◽  
A. Kato ◽  
K. Yamada ◽  
K. Matsumoto ◽  
...  
Keyword(s):  
Image Processing ◽  
Protein Synthesis ◽  
Focal Cerebral Ischemia ◽  
Cryostat Section ◽  
Processing System ◽  
Autoradiographic Study ◽  
Image Processing Technique ◽  
Image Subtraction ◽  
Trichloracetic Acid

The standard biochemical method of trichloracetic acid (TCA) wash and the image processing technique were combined to differentiate and visualize the distributions of polypeptide-incorporated and unincorporated tracers in an autoradiographic study of regional protein synthesis, The validity of applying TCA wash procedures to cryostat sections was considered by histologic and chemical evaluations, For the autoradiographic study of in vivo protein synthesis, a tracer dose of L-[14C]valine was administered 30 min after occlusion of the posterior communicating artery in gerbils. Images of total (polypeptide-incorporated and unincorporated) radioactivity and of polypeptide-incorporated radioactivity were obtained from an identical cryostat section before and after TCA wash. The polypeptide-unincorporated radioactivity image was produced with an image processing system by subtracting pixel by pixel the polypeptide-incorporated radioactivity from the total radioactivity. The present study clearly demonstrated that in spite of the sufficient delivery of tracer amino acids, the polypeptide synthesis was completely lost in the ischemic focus. Free tracer was markedly accumulated in the brain adjacent to the ischemic focus. This kind of autoradiographic technique seems to be indispensable in studying the topographical complexity of the altered protein metabolism in the pathologic brain.

scholarly journals Autoradiographic study of 3H colchicine binding in synchronously dividing cells of antheridial filaments of Chara vulgaris L. during successive stages of development

2015 ◽  
Vol 44 (1) ◽  
pp. 29-39 ◽  
Author(s):  
Maria J. Olszewska
Keyword(s):  
Protein Synthesis ◽  
Daughter Cell ◽  
Mitotic Cycle ◽  
Autoradiographic Study ◽  
Chara Vulgaris ◽  
Cytoplasmic Surface ◽  
Dividing Cells ◽  
Successive Generations ◽  
Mother Cells

The intensity of <sup>3</sup>H colchicine binding was investigated autoradiographically as a marker of an amount of the microtubule subunits during interphase and mitosis in synchronously dividing 4-, 8-, 16- and 32-celled antheridial filaments of <i>Chora vulgaris</i>. These cells were incubated with 3H colchicine in vivo or after fixation. The radioactivity of cells in the successive generations of antheridial filaments diminishes, similarly as the surface of cytoplasm and intensity of protein synthesis. During interphase the intensity of <sup>3</sup>H colchicine binding is proportional to the increase of cytoplasmic surface; the highest increase of radioactivity occurs in G<sub>2</sub>. During mitosis the increase of radioactivity continues in prophase; the highest radioactivity was found in prophase and telophase cells, the lowest in anaphase cells; a comparatively pronounced radioactivity is visible in metaphase. Radioactivity in posttelophase, as estimated per one daughter cell, is approximately one half of that of the mother cells in telophase of the previous generation suggesting the reutilization of microtubule proteins in the next mitotic cycle.

Development of Inspection System for IC Lead Frame Defects

1993 ◽  
Vol 5 (4) ◽  
pp. 369-373
Author(s):  
Hirokazu Tsuji ◽  
◽  
Kazuo Maruyama
Keyword(s):  
Image Processing ◽  
Feature Extraction ◽  
Pattern Matching ◽  
Processing System ◽  
Local Feature ◽  
Image Processing Technique ◽  
Inspection System ◽  
Lead Frame ◽  
Feature Extraction Method ◽  
Local Feature Extraction

A visual inspection system for IC lead frame defects based on the image processing technique is developed, and its inspection algorithm for the practical use is discussed. The inspection system consists of an image input system using a microscope and a TV camera, an image processing system, and a lead frame carrier. The inspection algorithm to recognize defects consists of pattern matching and local feature extraction. In the pattern matching method, minimum recognition size of defects is limited to the positioning error of the lead frame; therefore, small defects are recognized by the local feature extraction method in which local irregular patterns are detected using logical filter.

scholarly journals Control Design for Attitude and Position Based on Real Time Color Tracking System with Image Processing Technique

2020 ◽  
Vol 13 (2) ◽  
pp. 32
Author(s):  
Hsu Myat Tin Swe ◽  
Hla Myo Tun ◽  
Maung Maung Latt
Keyword(s):  
Image Processing ◽  
Real Time ◽  
Tracking System ◽  
Experimental Studies ◽  
Processing System ◽  
Control Design ◽  
Processing Technique ◽  
Sensor System ◽  
Image Processing Technique ◽  
Color Tracking

The paper mainly emphasizes on the control design for attitude and position based on real time color tracking system with image processing technique. The research problem in this study is to observe the high accuracy of the tracking system in image processing areas. The solution for this problem is to control the attitude and position of the object based on real time color tracking system. The objective of this study is to implement the image processing algorithms for autonomous tracking system. The specific objective of this study was fulfilled the experimental studies for contribution of real time color tracking for motion detection system in reality based on this study. This system is used the high performance camera to improve the enactment of tracking of a target and estimation of a motion. An image processing system consists of a light source to illuminate the sense, a sensor system, an interface between the sensor system and the computer. Then, color component analysis is used for color tracking system. MATLAB is competently used for tracking the ball and controlling the attitude and position of the ball.

scholarly journals Human Distance Estimation using Quadcopter for Surveillance Purpose

2019 ◽  
Vol 8 (12S2) ◽  
pp. 483-488
Keyword(s):  
Image Processing ◽  
Everyday Life ◽  
Unmanned Aerial Vehicles ◽  
Crime Rate ◽  
Processing System ◽  
Distance Estimation ◽  
Processing Technique ◽  
Image Processing Technique ◽  
Raspberry Pi ◽  
Aerial Vehicles

Nowadays, quadcopters are commonly used. Quadcopters are unmanned aerial vehicles with four propellers to provide lift to fly and hover above ground. Quadcopter nowadays is a very common commercial item in everyday life. Some quadcopters are designed to do 3D or 2D mapping of a certain area or to take videos or just for entertainment purposes. Quadcopter is a very versatile item and is able to change into anything for example a quadcopter can also be used for security purposes to decrease the crime rate of our country. The objective of this study is to design and develop a quadcopter with image processing system to have the ability to measure the distance of a human from the drone itself. The quadcopter is designed to be small in size and have a mini computer like Raspberry Pi on top of it to compute the algorithm to calculate the distance of the human by using image processing technique through the camera which is setup on the drone. Human detecting algorithm YOLO and software Open CV is chosen to detect human and calculate the distance from the quadcopter. The results show that the system is quite limited by the capabilities of the hardware. The system shows an accuracy of more than 90 percent when the human is standing within a certain range. Both the accuracy of the distance sensing and human recognizing system is affected by the limitation of the hardware.

scholarly journals Footprint-Based Health Monitoring Database using Raspberry PI

2020 ◽  
Vol 8 (6) ◽  
pp. 851-854
Keyword(s):  
Image Processing ◽  
Health Monitoring ◽  
Processing System ◽  
Processing Technique ◽  
Health Condition ◽  
Image Processing Technique ◽  
Raspberry Pi ◽  
Data Allocation ◽  
Color Analysis ◽  
The Web

The health monitoring of the person can be done in the different ways. The health of the patient can be determined by the image processing technique. The biometric parameters can gather the details of the health condition of the patient. The digital image processing can be applied in the various filed such as medical, geology, research etc., In this paper they proposes the foot print technology this can capture the foot print of the patient by using the web cam. The captured image can be analyzed by using the shape and the dimension analysis. The foot print can reads the each person identity. Based upon the identity and the numbers the image processing system is implemented. It uses the raspberry pi as the main part. The data which is captured by the web cam can be stored in the SD card. The data allocation is done in the memory path. The classification of the data is takes place by using the data separation algorithm. The color analysis can takes place a significant place based upon the color we can able to classify the foot print and makes it for further analysis. There are several steps can be took place the image acquisition, edge detection, feature extraction, pattern recognition, pattern matching. The matched image can be provided as the better result. Based upon the result the health condition can be predicted. This method is highly effective and accurate when compared to other method

Research and Development of Measurement Method for Structural Fracturing Process in Shake Table Tests Using Image Processing Technique

2004 ◽  
Vol 126 (1) ◽  
pp. 115-121 ◽  
Author(s):  
Satoshi Fujita ◽  
Osamu Furuya ◽  
Tadashi Mikoshiba
Keyword(s):  
Image Processing ◽  
Measurement Method ◽  
Three Dimensional ◽  
Processing System ◽  
Steel Structure ◽  
Processing Technique ◽  
Image Processing Technique ◽  
Shake Table ◽  
Shake Table Tests ◽  
Fracturing Process

The largest three-dimensional shake table is now being constructed in Hyogo prefecture, Japan for a solution of fracturing process of structures, buildings, and soils. However, it seems to be difficult to measure the fracturing processes of the structures during severe earthquake by using conventional methods and equipment, because the three-dimensional measurement of larger dynamic displacement in excess of elastic region of the structure will be the key to a solution and cannot be obtained by any of the vibration pick-ups such as displacement transducers and so on. In this study, R&D of the new measurement method to clarify the fracturing process of the structures by applying a so-called motion capture technique, which has been mainly studied for modeling of human actions and motions. This paper describes the concept of the system, the outline of the proto-type image processing system developed in the study and the results of the shake table test using five-story steel-structure model to investigate the measurable accuracy of the system.

An image processing technique for the observation of the viability of Steinernema carpocapsae in spray application research

Nematology ◽  
2010 ◽  
Vol 12 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Nicole Viaene ◽  
Winy Messens ◽  
David Nuyttens ◽  
Maurice Moens ◽  
Nancyde Sutter ◽  
...  
Keyword(s):  
Image Processing ◽  
Sodium Chloride ◽  
Entomopathogenic Nematodes ◽  
Processing System ◽  
Processing Technique ◽  
Image Processing Technique ◽  
Spray Application ◽  
Application Research ◽  
Image Processing System ◽  
The Difference

AbstractDamage caused to entomopathogenic nematodes by spray application is generally assessed by observing the viability of the infective juveniles under the microscope. To improve the quality and speed of this observation we developed an image processing technique and tested the efficacy of acetic acid and sodium chloride as chemical stimulants. Because of the lower standard error on the results obtained (0.7 vs 1.7), sodium chloride was eventually selected for all subsequent observations. The viability as observed with the image processing technique rose significantly with the time after the nematodes were suspended in water; however, viability as observed under the microscope was not influenced by the time. These differences can be attributed to the difference in type of stimulant (mechanical vs chemical) used. After nematodes had been in suspension for 3.5 h, the viability as measured using the image processing system was still significantly lower than the viability as measured under the microscope. This difference did not disappear after 24 h at 4, 15 or 24°C. Maintaining nematodes for 24 h at 35°C significantly decreased the viability to 5.9% (microscope) or 11.0% (image processing technique). The decrease in viability as observed with the image processing system corresponded better with the decrease in infectivity (i.e., 13.8%). Our results support further use of the image processing technique, not only to observe the viability of entomopathogenic nematodes but also to count the mobile or total number of nematodes of any species.

Development of Digital Image Processing System Based on MATLAB

2014 ◽  
Vol 971-973 ◽  
pp. 1594-1597 ◽  
Author(s):  
Ya Dong Zhang ◽  
Shun Min Zhu ◽  
Li Li
Keyword(s):  
Image Processing ◽  
Digital Image Processing ◽  
Digital Image ◽  
Scientific Research ◽  
Processing System ◽  
Processing Technique ◽  
Image Processing Technique ◽  
Image Processing System ◽  
Research And Education

Digital image processing is a technology that using computer to process images through a certain algorithm. It has been widely used in many areas of engineering and military. The system which is fit for the researching and teaching plays a very important role in the fields of scientific research and education. This paper discusses the development of image processing system and image processing technique in MATLAB. In practice, the operation of the system is convenient its interface is beautiful and its vision is very good.

A New Image Processing Technique for STEM

1974 ◽  
Vol 32 ◽  
pp. 432-433
Author(s):  
Yasushi Kokubo ◽  
Hirotami Koike ◽  
Teruo Someya
Keyword(s):  
Electron Microscopy ◽  
Image Processing ◽  
Scanning Electron Microscopy ◽  
Elastic Scattering ◽  
Field Emission ◽  
Processing Technique ◽  
Image Processing Technique ◽  
Energy Analyzer ◽  
Scanning Microscope ◽  
Scanning Electron

One of the advantages of scanning electron microscopy is the capability for processing the image contrast, i.e., the image processing technique. Crewe et al were the first to apply this technique to a field emission scanning microscope and show images of individual atoms. They obtained a contrast which depended exclusively on the atomic numbers of specimen elements (Zcontrast), by displaying the images treated with the intensity ratio of elastically scattered to inelastically scattered electrons. The elastic scattering electrons were extracted by a solid detector and inelastic scattering electrons by an energy analyzer. We noted, however, that there is a possibility of the same contrast being obtained only by using an annular-type solid detector consisting of multiple concentric detector elements.

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