scholarly journals Three-dimensional spatiotemporal focusing of holographic patterns

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Oscar Hernandez ◽  
Eirini Papagiakoumou ◽  
Dimitrii Tanese ◽  
Kevin Fidelin ◽  
Claire Wyart ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Sample Volume ◽  
Pattern Generation ◽  
Spatial Light Modulators ◽  
Two Photon ◽  
Light Modulators ◽  
Computer Generated Holography ◽  
Photon Excitation ◽  
Temporal Focusing ◽  
Axial Translation

Abstract Two-photon excitation with temporally focused pulses can be combined with phase-modulation approaches, such as computer-generated holography and generalized phase contrast, to efficiently distribute light into two-dimensional, axially confined, user-defined shapes. Adding lens-phase modulations to 2D-phase holograms enables remote axial pattern displacement as well as simultaneous pattern generation in multiple distinct planes. However, the axial confinement linearly degrades with lateral shape area in previous reports where axially shifted holographic shapes were not temporally focused. Here we report an optical system using two spatial light modulators to independently control transverse- and axial-target light distribution. This approach enables simultaneous axial translation of single or multiple spatiotemporally focused patterns across the sample volume while achieving the axial confinement of temporal focusing. We use the system's capability to photoconvert tens of Kaede-expressing neurons with single-cell resolution in live zebrafish larvae.

Two-Photon Excitation Imaging of Glucose Metabolism in Living Tissue

1997 ◽  
Vol 3 (S2) ◽  
pp. 305-306
Author(s):  
David W. Piston
Keyword(s):  
Confocal Microscopy ◽  
Collection Efficiency ◽  
Three Dimensional ◽  
Spatial Filter ◽  
Input Power ◽  
Photon Absorption ◽  
Focal Volume ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. It provides three-dimensional resolution and eliminates background equivalent to an ideal confocal microscope without requiring a confocal spatial filter, whose absence enhances fluorescence collection efficiency. This results in inherent submicron optical sectioning by excitation alone. In practice, TPEM is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10−5 limits the average input power to less than 10 mW, only slightly greater than the power normally used in confocal microscopy. Because of the intensity-squared dependence of the two-photon absorption, the excitation is limited to the focal volume.

scholarly journals Large size three-dimensional video by electronic holography using multiple spatial light modulators

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Hisayuki Sasaki ◽  
Kenji Yamamoto ◽  
Koki Wakunami ◽  
Yasuyuki Ichihashi ◽  
Ryutaro Oi ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Spatial Light Modulators ◽  
Light Modulators ◽  
Large Size ◽  
Electronic Holography

Crosstalk in Photoluminescence Readout of Three-Dimensional Memory in Vitreous Silica by One- and Two-Photon Excitation

2000 ◽  
Vol 39 (Part 1, No. 12A) ◽  
pp. 6763-6767 ◽  
Author(s):  
Mitsuru Watanabe ◽  
Saulius Juodkazis ◽  
Shigeki Matsuo ◽  
Junji Nishii ◽  
Hiroaki Misawa
Keyword(s):  
Three Dimensional ◽  
Vitreous Silica ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism

Nanophotonics ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 143-148
Author(s):  
Adrien Descloux ◽  
Marcel Müller ◽  
Vytautas Navikas ◽  
Andreas Markwirth ◽  
Robin van den Eynde ◽  
...  
Keyword(s):  
High Speed ◽  
Three Dimensional ◽  
Super Resolution ◽  
Optical Element ◽  
Spatial Light Modulators ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Image Stack ◽  
Light Modulators ◽  
3D Information

AbstractSuper-resolution structured illumination microscopy (SR-SIM) can be conducted at video-rate acquisition speeds when combined with high-speed spatial light modulators and sCMOS cameras, rendering it particularly suitable for live-cell imaging. If, however, three-dimensional (3D) information is desired, the sequential acquisition of vertical image stacks employed by current setups significantly slows down the acquisition process. In this work, we present a multiplane approach to SR-SIM that overcomes this slowdown via the simultaneous acquisition of multiple object planes, employing a recently introduced multiplane image splitting prism combined with high-speed SIM illumination. This strategy requires only the introduction of a single optical element and the addition of a second camera to acquire a laterally highly resolved 3D image stack. We demonstrate the performance of multiplane SIM by applying this instrument to imaging the dynamics of mitochondria in living COS-7 cells.

scholarly journals A Projection System for Real World Three-Dimensional Objects Using Spatial Light Modulators

2008 ◽  
Vol 4 (2) ◽  
pp. 254-261 ◽  
Author(s):  
Unnikrishnan Gopinathan ◽  
David S. Monaghan ◽  
Bryan M. Hennelly ◽  
Conor P. McElhinney ◽  
Damien P. Kelly ◽  
...  
Keyword(s):  
Real World ◽  
Three Dimensional ◽  
Spatial Light Modulators ◽  
Projection System ◽  
Three Dimensional Objects ◽  
Light Modulators

scholarly journals Synthesis of three-dimensional light fields with binary spatial light modulators

2011 ◽  
Vol 28 (6) ◽  
pp. 1211 ◽  
Author(s):  
Erdem Ulusoy ◽  
Levent Onural ◽  
Haldun M. Ozaktas
Keyword(s):  
Three Dimensional ◽  
Spatial Light Modulators ◽  
Light Fields ◽  
Light Modulators

scholarly journals How to optimize the absorption of two entangled photons

2021 ◽  
Vol 4 (4) ◽  
Author(s):  
Edoardo Carnio ◽  
Andreas Buchleitner ◽  
Frank Schlawin
Keyword(s):  
Pulse Shaping ◽  
Absorption Efficiency ◽  
Photon Absorption ◽  
Spatial Light Modulators ◽  
Schmidt Decomposition ◽  
Maximum Enhancement ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Light Modulators ◽  
Down Conversion

We investigate how entanglement can enhance two-photon absorption in a three-level system. First, we employ the Schmidt decomposition to determine the entanglement properties of the optimal two-photon state to drive such a transition, and the maximum enhancement which can be achieved in comparison to the optimal classical pulse. We then adapt the optimization problem to realistic experimental constraints, where photon pairs from a down-conversion source are manipulated by local operations such as spatial light modulators. We derive optimal pulse shaping functions to enhance the absorption efficiency, and compare the maximal enhancement achievable by entanglement to the yield of optimally shaped, separable pulses.

scholarly journals High Fidelity Spatial Light Modulator Configuration for Photo-Stimulation

2021 ◽  
Vol 9 ◽  
Author(s):  
Samira Aghayee ◽  
Mitchell Weikert ◽  
Phillip Alvarez ◽  
Gabriel A. Frank ◽  
Wolfgang Losert
Keyword(s):  
Spatial Light Modulator ◽  
Signal To Noise Ratio ◽  
Spatial Light Modulators ◽  
High Signal ◽  
High Fidelity ◽  
Light Modulator ◽  
Fourier Plane ◽  
Two Photon ◽  
Light Modulators ◽  
The Brain

For their capacity to shape optical wavefronts in real time into any desired illumination pattern, phase-only Spatial Light Modulators (SLM) have proven to be powerful tools for optical trapping and micromanipulation applications. SLMs are also becoming increasingly utilized in selective photo-stimulation of groups of neurons in the brain. However, conventional SLM based wavefront modulation introduces artifacts that are particularly detrimental for photo-stimulation applications. The primary issue is the unmodulated light that travels along the 0th order of diffraction. This portion of light is commonly blocked at the center of the object plane, which prevents photo-stimulation in the blocked region. We demonstrate a virtual lens configuration that moves the 1st order diffraction with the desired illumination pattern into the Fourier plane of the 0th order light. This virtual lens setup makes the whole field of view accessible for photo-stimulation and eliminates the need for removing the 0th order light in two-photon applications. Furthermore, in an example application to reconstruct a pattern consisting of an array of points, the virtual lens configuration increases the uniformity of the intensities these points. Moreover, diffraction-induced artifacts are also significantly reduced within the target plane. Therefore, our proposed high fidelity configuration yields target points with high signal to noise ratio.

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