Phage DNA Directed Enzyme Synthesis in in vitro System from Yeast Mitochondria

1972 ◽  
Vol 238 (81) ◽  
pp. 74-76 ◽  
Author(s):  
DIETMAR RICHTER ◽  
PETER HERRLICH ◽  
MANFRED SCHWEIGER
Keyword(s):  
Enzyme Synthesis ◽  
Yeast Mitochondria ◽  
In Vitro System ◽  
Phage Dna

scholarly journals 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria.

1988 ◽  
Vol 107 (6) ◽  
pp. 2051-2057 ◽  
Author(s):  
H Murakami ◽  
D Pain ◽  
G Blobel
Keyword(s):  
Heat Shock ◽  
Matrix Protein ◽  
Protein Import ◽  
Protein S ◽  
Yeast Mitochondria ◽  
Related Protein ◽  
Free System ◽  
In Vitro System ◽  
Cytosolic Factors

We have developed an in vitro system in which the posttranslational import of Put2 (delta-pyrroline-5-carboxylate dehydrogenase), into yeast mitochondria is dependent on the addition of yeast postribosomal supernatant (PRS). When mRNA for a nuclear-encoded yeast mitochondrial matrix protein, Put2, was translated in a wheat germ cell-free system, import into posttranslationally added yeast mitochondria was negligible. However, when a yeast PRS was added, significant import was observed. The import stimulating activity of the yeast PRS was shown to consist of at least two distinct factors. One of these is the recently purified 70-kD heat shock-related protein Ssalp/Ssa2p, two proteins that are 98% homologous. The other factor is an N-ethylmaleimide-sensitive protein(s). Both factors act synergistically.

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

The Mesothelial Cell as a Non-Thrombogenic Surface

1984 ◽  
Vol 52 (02) ◽  
pp. 102-104 ◽  
Author(s):  
L J Nicholson ◽  
J M F Clarke ◽  
R M Pittilo ◽  
S J Machin ◽  
N Woolf
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Flow ◽  
Cell Surface ◽  
Mesothelial Cell ◽  
Mesothelial Cells ◽  
Plastic Film ◽  
In Vitro System ◽  
Scanning Electron

SummaryA technique for harvesting mesothelial cells is described. This entails collagenase digestion of omentum after which the cells can be cultured. The technique has been developed using the rat, but has also been successfully applied to human tissue. Cultured rat mesothelial cells obtained in this way have been examined by scanning electron microscopy. Rat mesothelial cells grown on plastic film have been exposed to blood in an in vitro system using a Baumgartner chamber and have been demonstrated to support blood flow. No adhering platelets were observed on the mesothelial cell surface. Fibroblasts similarily exposed to blood as a control were washed off the plastic.

scholarly journals Multi-Organ toxicity demonstration in a functional human in vitro system composed of four organs

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Carlota Oleaga ◽  
Catia Bernabini ◽  
Alec S.T. Smith ◽  
Balaji Srinivasan ◽  
Max Jackson ◽  
...  
Keyword(s):  
In Vitro System ◽  
Organ Toxicity

scholarly journals In vitro asymmetric binding of the pleiotropic regulatory protein, FruR, to the ace operator controlling glyoxylate shunt enzyme synthesis.

1994 ◽  
Vol 269 (21) ◽  
pp. 14885-14891 ◽  
Author(s):  
J.C. Cortay ◽  
D. Nègre ◽  
M. Scarabel ◽  
T.M. Ramseier ◽  
N.B. Vartak ◽  
...  
Keyword(s):  
Regulatory Protein ◽  
Enzyme Synthesis ◽  
Glyoxylate Shunt

scholarly journals Evaluation of the bioaccessibility of a carotenoid beadlet blend using an in vitro system mimicking the upper gastrointestinal tract

2021 ◽  
Author(s):  
Chun Hu ◽  
Dawna Salter Venzon ◽  
Katja Lange ◽  
Annet Maathuis ◽  
Susann Bellmann ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Upper Gastrointestinal Tract ◽  
In Vitro System ◽  
Upper Gastrointestinal
Sign in / Sign up

Export Citation Format

Share Document