Nucleotide Metabolism

SummaryBlood from normal full term infants, mothers and normal adults was collected in citrate. Citrated platelet-rich plasma was prelabelled with 3H-adenine and reacted with release inducers, collagen and adrenaline. Adenine nucleotide metabolism, total adenine nucleotide levels and changes in sizes of these pools were determined in platelets from these three groups of subjects.At rest, the platelet of the newborn infant, compared to that of the mother and normal adult, possessed similar amounts of adenosine triphosphate (ATP), 4.6 ± 0.2 (SD), 5.0 ± 1.1, 4.9 ± 0.6 µmoles ATP/1011 platelets respectively, and adenosine diphosphate (ADP), 2.4 ± 0.7, 2.8 ± 0.6, 3.0 ± 0.3 umoles ADP/1011 platelets respectively. However the marked elevation of specific radioactivity of ADP and ATP in these resting platelets indicated the platelet of the neonate has decreased adenine nucleotide stores.In addition to these decreased stores of adenine nucleotides, infant platelets showed significantly impaired release of ADP and ATP on exposure to collagen. The release of ADP in infants, mothers, and other adults was 0.9 ± 0.5 (SD), 1.5 ± 0.5, 1.5 ± 0.1 umoles/1011 platelets respectively; that of ATP was 0.6 ± 0.3, 1.0 ± 0.1,1.3 ± 0.2 µmoles/1011 platelets respectively. With collagen-induced release, platelets of newborn infants compared to those of other subjects showed only slight increased specific radioactivities of adenine nucleotides over basal levels. The content of metabolic hypoxanthine, a breakdown product of adenine nucleotides, increased in both platelets and plasma in all subjects studied.In contrast, with adrenaline as release inducer, the platelets of the newborn infant showed no adenine nucleotide release, no change in total ATP and level of radioactive hypoxanthine, and minimal change in total ADP. The reason for this decreased adrenaline reactivity of infant platelets compared to reactivity of adult platelets is unknown.Infant platelets may have different membranes, with resulting differences in regulation of cellular processes, or alternatively, may be refractory to catecholamines because of elevated levels of circulating catecholamines in the newborn period.

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Nonenzymatic glycosylation of erythrocytic proteins in normal and diabetic subjects. Enzymes of nucleoside and nucleotide metabolism

Diabetes ◽

10.2337/diabetes.34.3.251 ◽

1985 ◽

Vol 34 (3) ◽

pp. 251-255 ◽

Cited By ~ 2

Author(s):

K. C. Agarwal ◽

R. E. Parks ◽

J. A. Widness ◽

R. Schwartz

Keyword(s):

Nucleotide Metabolism ◽

Nonenzymatic Glycosylation

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Deoxythymidine nucleotide metabolism in Bacillus subtilis W23 infected with bacteriophage SP1Oc: preliminary evidence that dTMP in SP10c DNA is synthesized by a novel, bacteriophage-specific mechanism.

Journal of Virology ◽

10.1128/jvi.29.1.61-68.1979 ◽

1979 ◽

Vol 29 (1) ◽

pp. 61-68 ◽

Cited By ~ 2

Author(s):

O Markewych ◽

E Casella ◽

M Dosmar ◽

H Witmer

Keyword(s):

Bacillus Subtilis ◽

Preliminary Evidence ◽

Nucleotide Metabolism ◽

Specific Mechanism

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Whole-Genome Doubling Affects Pre-miRNA Expression in Plants

Plants ◽

10.3390/plants10051004 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1004

Author(s):

Salvatore Esposito ◽

Riccardo Aversano ◽

Pasquale Tripodi ◽

Domenico Carputo

Keyword(s):

Dna Repair ◽

Mirna Expression ◽

Building Blocks ◽

Nucleotide Metabolism ◽

Integrative Approach ◽

Whole Genome ◽

Solanum Commersonii ◽

Genome Doubling ◽

Whole Genome Doubling ◽

Micro Rnas

Whole-genome doubling (polyploidy) is common in angiosperms. Several studies have indicated that it is often associated with molecular, physiological, and phenotypic changes. Mounting evidence has pointed out that micro-RNAs (miRNAs) may have an important role in whole-genome doubling. However, an integrative approach that compares miRNA expression in polyploids is still lacking. Here, a re-analysis of already published RNAseq datasets was performed to identify microRNAs’ precursors (pre-miRNAs) in diploids (2x) and tetraploids (4x) of five species (Arabidopsis thaliana L., Morus alba L., Brassica rapa L., Isatis indigotica Fort., and Solanum commersonii Dun). We found 3568 pre-miRNAs, three of which (pre-miR414, pre-miR5538, and pre-miR5141) were abundant in all 2x, and were absent/low in their 4x counterparts. They are predicted to target more than one mRNA transcript, many belonging to transcription factors (TFs), DNA repair mechanisms, and related to stress. Sixteen pre-miRNAs were found in common in all 2x and 4x. Among them, pre-miRNA482, pre-miRNA2916, and pre-miRNA167 changed their expression after polyploidization, being induced or repressed in 4x plants. Based on our results, a common ploidy-dependent response was triggered in all species under investigation, which involves DNA repair, ATP-synthesis, terpenoid biosynthesis, and several stress-responsive transcripts. In addition, an ad hoc pre-miRNA expression analysis carried out solely on 2x vs. 4x samples of S. commersonii indicated that ploidy-dependent pre-miRNAs seem to actively regulate the nucleotide metabolism, probably to cope with the increased requirement for DNA building blocks caused by the augmented DNA content. Overall, the results outline the critical role of microRNA-mediated responses following autopolyploidization in plants.

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ATF3 coordinates serine and nucleotide metabolism to drive cell cycle progression in acute myeloid leukemia

Molecular Cell ◽

10.1016/j.molcel.2021.05.008 ◽

2021 ◽

Author(s):

Daniela Di Marcantonio ◽

Esteban Martinez ◽

Joice S. Kanefsky ◽

Jacklyn M. Huhn ◽

Rashid Gabbasov ◽

...

Keyword(s):

Cell Cycle ◽

Acute Myeloid Leukemia ◽

Myeloid Leukemia ◽

Cell Cycle Progression ◽

Nucleotide Metabolism ◽

Cycle Progression ◽

Acute Myeloid

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Nucleotide Metabolism

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)68046-4 ◽

1958 ◽

Vol 233 (1) ◽

pp. 154-158

Author(s):

John H. Schneider ◽

Van R. Potter

Keyword(s):

Nucleotide Metabolism

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A Label-Free Cellular Proteomics Approach to Decipher the Antifungal Action of DiMIQ, a Potent Indolo[2,3-b]Quinoline Agent, against Candida albicans Biofilms

International Journal of Molecular Sciences ◽

10.3390/ijms22010108 ◽

2020 ◽

Vol 22 (1) ◽

pp. 108

Author(s):

Robert Zarnowski ◽

Anna Jaromin ◽

Agnieszka Zagórska ◽

Eddie G. Dominguez ◽

Katarzyna Sidoryk ◽

...

Keyword(s):

Extracellular Matrix ◽

Candida Albicans ◽

Ribosomal Proteins ◽

Nucleotide Metabolism ◽

Antibiofilm Activity ◽

Label Free ◽

Xtt Assay ◽

Fungal Cell ◽

Select Amino Acid ◽

Cellular Oxidation

Candida albicans forms extremely drug-resistant biofilms, which present a serious threat to public health globally. Biofilm-based infections are difficult to treat due to the lack of efficient antifungal therapeutics, resulting in an urgent demand for the development of novel antibiofilm strategies. In this study, the antibiofilm activity of DiMIQ (5,11-dimethyl-5H-indolo[2,3-b]quinoline) was evaluated against C. albicans biofilms. DiMIQ is a synthetic derivative of indoquinoline alkaloid neocryptolepine isolated from a medicinal African plant, Cryptolepis sanguinolenta. Antifungal activity of DiMIQ was determined using the XTT assay, followed by cell wall and extracellular matrix profiling and cellular proteomes. Here, we demonstrated that DiMIQ inhibited C. albicans biofilm formation and altered fungal cell walls and the extracellular matrix. Cellular proteomics revealed inhibitory action against numerous translation-involved ribosomal proteins, enzymes involved in general energy producing processes and select amino acid metabolic pathways including alanine, aspartate, glutamate, valine, leucine and isoleucine. DiMIQ also stimulated pathways of cellular oxidation, metabolism of carbohydrates, amino acids (glycine, serine, threonine, arginine, phenylalanine, tyrosine, tryptophan) and nucleic acids (aminoacyl-tRNA biosynthesis, RNA transport, nucleotide metabolism). Our findings suggest that DiMIQ inhibits C. albicans biofilms by arresting translation and multidirectional pathway reshaping of cellular metabolism. Overall, this agent may provide a potent alternative to treating biofilm-associated Candida infections.

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