scholarly journals Nanophotonics enhanced coverslip for phase imaging in biology

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Lukas Wesemann ◽  
Jon Rickett ◽  
Jingchao Song ◽  
Jieqiong Lou ◽  
Elizabeth Hinde ◽  
...  
Keyword(s):  
Human Cancer ◽  
Three Dimensional ◽  
Live Cells ◽  
Phase Imaging ◽  
Human Cancer Cells ◽  
Nanophotonic Devices ◽  
Optical Generation ◽  
Transparent Objects ◽  
Medical Diagnostic ◽  
Intensity Images

AbstractThe ability to visualise transparent objects such as live cells is central to understanding biological processes. Here we experimentally demonstrate a novel nanostructured coverslip that converts phase information to high-contrast intensity images. This compact device enables real-time, all-optical generation of pseudo three-dimensional images of phase objects on transmission. We show that by placing unstained human cancer cells on the device, the internal structure within the cells can be clearly seen. Our research demonstrates the significant potential of nanophotonic devices for integration into compact imaging and medical diagnostic devices.

scholarly journals Potential anticancer activity of curcumin analogs containing sulfone on human cancer cells

2016 ◽  
Vol 68 (1) ◽  
pp. 125-133 ◽  
Author(s):  
Qiuyan Zhang ◽  
Dongli Li ◽  
Yue Liu ◽  
Hui Wang ◽  
Changyuan Zhang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Human Cancer ◽  
Three Dimensional ◽  
In Vivo Studies ◽  
Anticancer Activities ◽  
Human Cancer Cells ◽  
Curcumin Analogs ◽  
Phosphorylated Akt ◽  
Transcription 3

Three curcumin analogs(S1-S3) containing sulfone were investigated for their effects on human prostate cancer PC-3, colon cancer HT-29, lung cancer H1299 and pancreatic cancer BxPC-3 cells. The three compounds were approximately 16-to 96-fold more active than curcumin in these cell lines as determined by the MTT assay. The effects of these compounds on cell growth were further studied in prostate cancer PC-3 cells in both two dimensional (2D) and three dimensional (3D) cultures. S1-S3strongly inhibited the growth and induced cell death in PC-3 cells, and the effects of these compounds were associated with suppression of nuclear factor kappa B (NF-?B) transcriptional activity. Moreover, treatment of PC-3 cells with all three compounds caused a decrease in the level of phosphorylated signal transducer and activator of transcription-3 (p-STAT3) (Tyr705),but not p-STAT3(Ser727). Only S1and S2decreased the presence of phosphorylated Akt (p-Akt) in PC-3 cells. These curcumin analogs warrant further in vivo studies for anticancer activities in suitable animal models.

scholarly journals From digital holographic microscopy to optical coherence tomography – separate past and a common goal

2021 ◽  
Vol 13 (4) ◽  
pp. 91
Author(s):  
Arkadiusz Kuś ◽  
Wojciech Krauze ◽  
Małgorzata Kujawińska
Keyword(s):  
Optical Coherence Tomography ◽  
Three Dimensional ◽  
Optical Diffraction ◽  
Phase Imaging ◽  
Diffraction Tomography ◽  
Optical Coherence ◽  
Quantitative Phase Imaging ◽  
Isotropic Resolution ◽  
Quantitative Phase ◽  
Transparent Objects

In this paper we briefly present the history and outlook on the development of two seemingly distant techniques which may be brought close together with a unified theoretical model described as common k-space theory. This theory also known as the Fourier diffraction theorem is much less common in optical coherence tomography than its traditional mathematical model, but it has been extensively studied in digital holography and, more importantly, optical diffraction tomography. As demonstrated with several examples, this link is one of the important factors for future development of both techniques. Full Text: PDF ReferencesN. Leith, J. Upatnieks, "Reconstructed Wavefronts and Communication Theory", J. Opt. Soc. Am. 52(10), 1123 (1962). CrossRef Y. Park, C. Depeursinge, G. Popescu, "Quantitative phase imaging in biomedicine", Nat. Photonics 12, 578 (2018). CrossRef D. Huang et al., "Optical Coherence Tomography", Science 254(5035), 1178 (1991). CrossRef D. P. Popescu, C. Flueraru, S. Chang, J. Disano, S. Sherif, M.G. Sowa, "Optical coherence tomography: fundamental principles, instrumental designs and biomedical applications", Biophys. Rev. 3(3), 155 (2011). CrossRef M. Wojtkowski, V. Srinivasan, J.G. Fujimoto, T. Ko, J.S. Schuman, A. Kowalczyk, J.S. Duker, "Three-dimensional Retinal Imaging with High-Speed Ultrahigh-Resolution Optical Coherence Tomography", Ophthalmology 112(10), 1734 (2005). CrossRef K.C. Zhou, R. Qian, A.-H. Dhalla, S. Farsiu, J.A. Izatt, "Unified k-space theory of optical coherence tomography", Adv. Opt. Photon. 13(2), 462 (2021). CrossRef A.F. Fercher, C.K. Hitzenberger, G. Kamp, S.Y. El-Zaiat, "Measurement of intraocular distances by backscattering spectral interferometry", Opt. Comm. 117(1-2), 43 (1995). CrossRef E. Wolf, "Determination of the Amplitude and the Phase of Scattered Fields by Holography", J. Opt. Soc. Am. 60(1), 18 (1970). CrossRef E. Wolf, "Three-dimensional structure determination of semi-transparent objects from holographic data", Opt. Comm. 1(4), 153 (1969). CrossRef V. Balasubramani et al., "Roadmap on Digital Holography-Based Quantitative Phase Imaging", J. Imaging 7(12), 252 (2021). CrossRef A. Kuś, W. Krauze, P.L. Makowski, M. Kujawińska, "Holographic tomography: hardware and software solutions for 3D quantitative biomedical imaging (Invited paper)", ETRI J. 41(1), 61 (2019). CrossRef A. Kuś, M. Dudek, M. Kujawińska, B. Kemper, A. Vollmer, "Tomographic phase microscopy of living three-dimensional cell cultures", J. Biomed. Opt. 19(4), 46009 (2014). CrossRef O. Haeberlé, K. Belkebir, H. Giovaninni, A. Sentenac, "Tomographic diffractive microscopy: basics, techniques and perspectives", J. Mod. Opt. 57(9), 686 (2010). CrossRef B. Simon et al., "Tomographic diffractive microscopy with isotropic resolution", Optica 4(4), 460 (2017). CrossRef B.A. Roberts, A.C. Kak, "Reflection Mode Diffraction Tomography", Ultrason. Imag. 7, 300 (1985). CrossRef M. Sarmis et al., "High resolution reflection tomographic diffractive microscopy", J. Mod. Opt. 57(9), 740 (2010). CrossRef L. Foucault et al., "Versatile transmission/reflection tomographic diffractive microscopy approach", J. Opt. Soc. Am. A 36(11), C18 (2019). CrossRef W. Krauze, P. Ossowski, M. Nowakowski, M. Szkulmowski, M. Kujawińska, "Enhanced QPI functionality by combining OCT and ODT methods", Proc. SPIE 11653, 116530B (2021). CrossRef E. Mudry, P.C. Chaumet, K. Belkebir, G. Maire, A. Sentenac, "Mirror-assisted tomographic diffractive microscopy with isotropic resolution", Opt. Lett. 35(11), 1857 (2010). CrossRef P. Hosseini, Y. Sung, Y. Choi, N. Lue, Z. Yaqoob, P. So, "Scanning color optical tomography (SCOT)", Opt. Expr. 23(15), 19752 (2015). CrossRef J. Jung, K. Kim, J. Yoon, Y. Park, "Hyperspectral optical diffraction tomography", Opt. Expr. 24(3), 1881 (2016). CrossRef T. Zhang et al., Biomed. "Multi-wavelength multi-angle reflection tomography", Opt. Expr. 26(20), 26093 (2018). CrossRef R.A. Leitgeb, "En face optical coherence tomography: a technology review [Invited]", Biomed. Opt. Expr. 10(5), 2177 (2019). CrossRef J.F. de Boer, R. Leitgeb, M. Wojtkowski, "Twenty-five years of optical coherence tomography: the paradigm shift in sensitivity and speed provided by Fourier domain OCT [Invited]", Biomed. Opt. Expr. 8(7), 3248 (2017). CrossRef T. Anna, V. Srivastava, C. Shakher, "Transmission Mode Full-Field Swept-Source Optical Coherence Tomography for Simultaneous Amplitude and Quantitative Phase Imaging of Transparent Objects", IEEE Photon. Technol. Lett. 23(11), 899 (2011). CrossRef M.T. Rinehart, V. Jaedicke, A. Wax, "Quantitative phase microscopy with off-axis optical coherence tomography", Opt. Lett. 39(7), 1996 (2014). CrossRef C. Photiou, C. Pitris, "Dual-angle optical coherence tomography for index of refraction estimation using rigid registration and cross-correlation", J. Biomed. Opt. 24(10), 1 (2019). CrossRef Y. Zhou, K.K.H. Chan, T. Lai, S. Tang, "Characterizing refractive index and thickness of biological tissues using combined multiphoton microscopy and optical coherence tomography", Biomed. Opt. Expr. 4(1), 38 (2013). CrossRef K.C. Zhou, R. Qian, S. Degan, S. Farsiu, J.A. Izatt, "Optical coherence refraction tomography", Nat. Photon. 13, 794 (2019). CrossRef

scholarly journals Two-dimensional and three-dimensional cell culture models in vitro: pros and cons

2018 ◽  
Vol 17 (3) ◽  
pp. 188-196
Author(s):  
E. S. Galimova ◽  
M. М. Galagudza
Keyword(s):  
Cell Culture ◽  
Human Cancer ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Two Dimensional ◽  
Human Cancer Cells ◽  
Culture Models ◽  
Pros And Cons ◽  
Molecular Aspects

Discovery and development of new chemical compounds with putative anti-cancer properties requires reliable predictive preclinical models for in vitro screening of efficacy. Such models mainly include cultures of human cancer cells: two-dimensional (2D) and three-dimensional (3D) cell culture systems. In this review, we discuss the molecular aspects of cells cultured in 2D and 3D, and their relevance to cancer study, focusing on key examples from the recent literature. Advantages, disadvantages and perspectives of described models are also analyzed.

Performing Quantitative Nanomechanical AFM Measurements on Live Cells

2013 ◽  
Vol 21 (6) ◽  
pp. 12-17 ◽  
Author(s):  
Bede Pittenger ◽  
Andrea Slade
Keyword(s):  
Three Dimensional ◽  
Force Spectroscopy ◽  
Live Cells ◽  
Phase Imaging ◽  
Operating Characteristics ◽  
Force Microscopy ◽  
Wide Range ◽  
Quantitative Manner ◽  
Automated Tools ◽  
Force Volume

Atomic force microscopy (AFM) has been recognized since the mid-eighties as an excellent technique to image a wide range of samples in their near-natural environment. Although the primary function of AFM is to generate three-dimensional (3D) profiles of the scanned surface, much more information can be delivered via this technique. In 1993, TappingMode was developed, which prevents tip and sample damage due to friction and shear forces and allows qualitative mechanical property mapping through phase imaging. About the same time, force spectroscopy and force volume (FV) were developed to study tip-sample forces at a point or over an area, respectively. To date, force spectroscopy and FV are the most commonly used AFM modes for measuring nanometer-scale mechanical forces in a quantitative manner. Unfortunately, force spectroscopy and FV suffer from slow acquisition speed and a lack of automated tools; these operating characteristics limit their use because of the hundreds or thousands of curves that are required for good statistics.

scholarly journals Measurement of the Imaginary Part of the Clausius-Mossotti Factor of Particle/Cell via Dual Frequency Electrorotation

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 329 ◽  
Author(s):  
Yung-Yi Lin ◽  
Ying-Jie Lo ◽  
U Lei
Keyword(s):  
Imaginary Part ◽  
Human Cancer ◽  
Three Dimensional ◽  
Optical Tweezer ◽  
Travelling Wave ◽  
Dual Frequency ◽  
Two Phase ◽  
Laser Tweezer ◽  
Human Cancer Cells ◽  
Phase Signal

A simple and inexpensive method using planar electrodes was proposed for the measurement of the imaginary part of the Clausius-Mossotti factor, K i , of particle/cell for electrorotation (ER) and travelling wave dielectrophoresis (twDEP). It is based on the balance between the dielectrophoretic and viscous torques on a particle undergoing ER subject to dual frequency operation in an ER chamber. A four-phase ac voltage signal with a given frequency is applied for generating ER for measurement, and another two-phase signal is applied at a selected frequency for generating a negative dielectrophoretic force for confining the particle motion, instead of using laser tweezer or three-dimensional electrodes in the literature. Both frequencies can be applied to the same electrodes in a four-electrode ER system and to alternative different electrodes in an eight-electrode ER system, and both systems are capable for providing accurate measurement. The measurements were validated by comparing with the theoretical result using sephadex particles in KCl solution, and with the existing experimental results for various human cancer cells in medium with conductivity from 0.01–1.2 S/m, using ER with optical tweezer and dual frequency twDEP. Contrast between the ER and the twDEP methods (the current two available methods) was discussed and commented. The present method could provide measurement for wider frequency range and more accurate result near K i = 0, in comparison with the results using the twDEP method. However, the twDEP method could perform much more rapid measurement. Detailed forces and torque were calculated inside the ER chamber for understanding the physics and assessing the characteristics of the dual frequency ER method. This study is of academic interest as the torque in ER and the force in twDEP can be calculated only when K i is known. It also finds biomedical applications as the K i -spectra can be served as physical phenotypes for different cells, and can be applied for deriving dielectric properties of cells.

scholarly journals Perfused Three-dimensional Organotypic Culture of Human Cancer Cells for Therapeutic Evaluation

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Xiao Wan ◽  
Steven Ball ◽  
Frances Willenbrock ◽  
Shaoyang Yeh ◽  
Nikola Vlahov ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Organotypic Culture ◽  
Human Cancer ◽  
Three Dimensional ◽  
Human Cancer Cells ◽  
Therapeutic Evaluation

A new method for the three-dimensional in vitro growth of human cancer cells

1983 ◽  
Vol 147 (2) ◽  
pp. 454-460 ◽  
Author(s):  
H KUPCHIK ◽  
R LANGER ◽  
C HABERERN ◽  
S ELDERINY ◽  
M OBRIEN
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Three Dimensional ◽  
New Method ◽  
In Vitro Growth ◽  
Human Cancer Cells
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