Single-cell EMT-related transcriptional analysis revealed intra-cluster heterogeneity of tumor cell clusters in epithelial ovarian cancer ascites

Oncogene ◽  
2020 ◽  
Vol 39 (21) ◽  
pp. 4227-4240
Author(s):  
Tongtong Kan ◽  
Wei Wang ◽  
Philip P. Ip ◽  
Shengtao Zhou ◽  
Alice S. Wong ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Tumor Cell ◽  
Epithelial Ovarian Cancer ◽  
Single Cell ◽  
Transcriptional Analysis ◽  
Cell Clusters

scholarly journals Gravity-based microfiltration reveals unexpected prevalence of circulating tumor cell clusters in ovarian cancer

2019 ◽  
Author(s):  
Anne Meunier ◽  
Javier Alejandro Hernández-Castro ◽  
Sara Kheireddine ◽  
Sara Al Habyan ◽  
Benjamin Péant ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Tumor Cell ◽  
Epithelial Ovarian Cancer ◽  
Single Cell ◽  
Tumor Cells ◽  
Mouse Models ◽  
Metastatic Potential ◽  
Circulating Tumor Cell ◽  
Large Size ◽  
Localized Disease

SummaryCirculating tumor cells (CTCs) are rare (few cells per milliliter of blood) and mostly isolated as single cell CTCs (scCTCs). CTC clusters (cCTCs), even rarer, are of growing interest, notably because of their higher metastatic potential, but very difficult to isolate. Here, we introduce gravity-based microfiltration (GµF) for facile isolation of cCTCs. We identify cluster break-up as a confounding cause, and achieve ∼85% capture efficiency. GµF from orthotopic ovarian cancer mouse models and from 10 epithelial ovarian cancer (EOC) patients uncovered cCTCs in every case, with between 2-100+ cells. cCTCs represented between 5-30% of all CTC events, and 10-80% of captured CTCs were clustered; remarkably, in two patients, more CTCs were circulating as cCTCs than scCTCs. GµF uncovered the unexpected prevalence, frequency and sometimes large size of cCTCs in EOC patients with either metastatic and localized disease, and motivates additional studies to uncover their properties and role in disease progression.

scholarly journals An Immunocompetent, Orthotopic Mouse Model of Epithelial Ovarian Cancer Utilizing Tissue Engineered Tumor Cell Sheets

2015 ◽  
Vol 21 (1) ◽  
pp. 23-34 ◽  
Author(s):  
Ayumi Arauchi ◽  
Chieh-Hsiang Yang ◽  
Sungpil Cho ◽  
Elke A. Jarboe ◽  
C. Matthew Peterson ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Mouse Model ◽  
Tumor Cell ◽  
Epithelial Ovarian Cancer ◽  
Cell Sheets ◽  
Orthotopic Mouse Model

scholarly journals Sam68 is Overexpressed in Epithelial Ovarian Cancer and Promotes Tumor Cell Proliferation

2016 ◽  
Vol 22 ◽  
pp. 3248-3256 ◽  
Author(s):  
Lijuan Dong ◽  
Hailuo Che ◽  
Mingmei Li ◽  
Xuepeng Li
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Tumor Cell ◽  
Epithelial Ovarian Cancer ◽  
Tumor Cell Proliferation

Single-cell RNA-seq recognized the initiator of epithelial ovarian cancer recurrence

Oncogene ◽  
2022 ◽  
Author(s):  
Tongtong Kan ◽  
Shupeng Zhang ◽  
Shengtao Zhou ◽  
Ya Zhang ◽  
Yun Zhao ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Single Cell ◽  
Cancer Recurrence ◽  
Rna Seq
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