Exosomal microRNA-503-3p derived from macrophages represses glycolysis and promotes mitochondrial oxidative phosphorylation in breast cancer cells by elevating DACT2

AbstractMicroRNAs (miRNAs) are emerging drivers in tumor progression, while the role of miR-503-3p in breast cancer (BC) remains largely unknown. We aimed to explore the impact of macrophage-derived exosomal miR-503-3p in the development of BC by regulating disheveled-associated binding antagonist of beta-catenin 2 (DACT2). miR-503-3p and DACT2 expression in BC tissues and cells was assessed, and the expression of Wnt/β-catenin signaling pathway-related proteins in BC cells was also evaluated. Macrophages were induced and exosomes were extracted. The screened BC cell lines were, respectively, treated with exosomes, miR-503-3p inhibitor/mimic or upregulated/inhibited DACT2, and then the phenotypes, glucose intake, oxygen consumption rate, and adenosine-triphosphate (ATP) level of BC cells were determined. Cell growth in vivo was also observed. MiR-503-3p was elevated, DACT2 was reduced, and Wnt/β-catenin signaling pathway was activated in BC cells. Macrophage-derived exosomes, upregulated miR-503-3p or inhibited DACT2 promoted malignant behaviors of BC cells, glucose intake, and activity of the Wnt/β-catenin signaling pathway, while repressed oxygen consumption rate and ATP level in BC cells. Reversely, reduced miR-503-3p or upregulated DACT2 exerted opposite effects. This study revealed that reduction of macrophage-derived exosomal miR-503-3p repressed glycolysis and promoted mitochondrial oxidative phosphorylation in BC by elevating DACT2 and inactivating Wnt/β-catenin signaling pathway. Our research may provide novel targets for BC treatment.

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The impact of high and low atmospheric pressure in the Jordanian environment on some physiological variable for runners .

Journal of Sociological Research ◽

10.5296/jsr.v4i2.4358 ◽

2013 ◽

Vol 4 (2) ◽

pp. 364

Author(s):

Baker Thnibat

Keyword(s):

Oxygen Consumption ◽

Energy Expenditure ◽

Atmospheric Pressure ◽

Oxygen Consumption Rate ◽

Consumption Rate ◽

Maximal Oxygen Consumption ◽

Training Groups ◽

Low Atmospheric Pressure ◽

The Impact ◽

High Atmospheric Pressure

The study aimed at identifying the impact of difference between the areas of high atmospheric pressure in the Jordanian environment on some physiological variable ( estimating maximal oxygen consumption , estimating rate of Energy expenditure, and hemoglobin ,vital capacity), for runners . The study sample consisted of (16) athletes who practice ( middle and long – distance running). A number of statistical methods were used to answer the questions of the study ( arithmetic means , standard deviations , Mann.Whiteny test ,and the result of Kolmogorov – Smirnov tests ) . The study result concluded that there was apriority for the measurements which were conducted on the training groups of low atmospheric pressure in comparison with the other group, with regard to the variables (maximal oxygen consumption , rate of Energy expenditure, and hemoglobin). The study result also showed that there was apriority for the measurements which were conducted on the training groups of high atmospheric pressure in comparison with the other group, regarding to the variables (maximal oxygen consumption , rate of Energy expenditure, and hemoglobin).in the areas of high atmospheric pressure.The study result also suggested that ther were no statistically significant differences between two training groups with regard to the study variables of (maximal oxygen consumption , rate of Energy expenditure, and hemoglobin ,vital capacity), were measured in of high atmospheric pressure areas .The study recommended about building sports facilities that simulate the areas of low atmospheric pressure , because of the expected role that the factors would play in improving some physiological variables .

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A novel estimate of ocean oxygen utilisation points to a reduced rate of respiration in the ocean interior

Biogeosciences ◽

10.5194/bg-10-7723-2013 ◽

2013 ◽

Vol 10 (11) ◽

pp. 7723-7738 ◽

Cited By ~ 34

Author(s):

O. Duteil ◽

W. Koeve ◽

A. Oschlies ◽

D. Bianchi ◽

E. Galbraith ◽

...

Keyword(s):

Oxygen Consumption ◽

Oxygen Consumption Rate ◽

Consumption Rate ◽

Ventilation Rate ◽

Upper Ocean ◽

Observational Dataset ◽

Oxygen Utilisation ◽

Ocean Models ◽

Reduced Rate ◽

The Impact

Abstract. The Apparent Oxygen Utilisation (AOU) is a classical measure of the amount of oxygen respired in the ocean's interior. We show that AOU systematically overestimates True Oxygen Utilisation (TOU) in 6 coupled circulation-biogeochemical ocean models. This is due to atmosphere–ocean oxygen disequilibria in the subduction regions, consistent with previous work. We develop a simple, new, observationally-based approach which we call Evaluated Oxygen Utilisation (EOU). In this approach, we take into account the impact of the upper ocean oxygen disequilibria into the interior, considering that transport takes place predominantly along isopycnal surfaces. The EOU approximates the TOU with less than half of the bias of AOU in all 6 models despite large differences in the physical and biological components of the models. Applying the EOU approach to a global observational dataset yields an oxygen consumption rate 25% lower than that derived from AOU-based estimates, for a given ventilation rate.

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Oxygen consumption rate in Gasterosteus aculeatus-Schistocephalus solidus system from a non-migratory naturally infected population

10.1101/111112 ◽

2017 ◽

Author(s):

Tarallo Andrea ◽

D’Onofrio Giuseppe ◽

Agnisola Claudio

Keyword(s):

Oxygen Consumption ◽

Oxygen Consumption Rate ◽

Gasterosteus Aculeatus ◽

Consumption Rate ◽

Host Fish ◽

Schistocephalus Solidus ◽

Rate Of Oxygen Consumption ◽

Migratory Population ◽

Host Parasite ◽

The Impact

AbstractThe three spine stickleback Gasterosteus aculeatus is a specific obligatory intermediate host for the cestode worm Schistocephalus solidus. This system is commonly used to investigate the host-parasite interaction in fishes. Despite the interesting attempts which have been made to quantify the impact of the parasite over the respiration rate of the host fish, none of the previous reports took in consideration that stickleback is diversified in different ecotypes according to its ability to made reproductive migration, from and to the sea. Here the oxygen consumption rate in specimens of three-spine stickleback collected from a non-migratory population was quantified with the aim to test if the S. solidus infection drives a change in the oxygen consumption level of the host fish. The results showed that the infected fishes have a higher rate of oxygen consumption compared with the uninfected one. The differences were due to a direct effect of the parasite, not merely to its contribution to the whole oxygen consumption rate. The data were compared with previous reports, showing that the non-migratory population was characterized by a different level of oxygen consumption rate. The differences were interpreted in terms of divergence in physiological adaptations which had to be appeared in different populations.

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Dual-emissive, oxygen-sensing boron nanoparticles quantify oxygen consumption rate in breast cancer cells

Journal of Biomedical Optics ◽

10.1117/1.jbo.25.11.116504 ◽

2020 ◽

Vol 25 (11) ◽

Author(s):

Ashlyn G. Rickard ◽

Meng Zhuang ◽

Christopher A. DeRosa ◽

Xiaojie Zhang ◽

Mark W. Dewhirst ◽

...

Keyword(s):

Breast Cancer ◽

Oxygen Consumption ◽

Cancer Cells ◽

Oxygen Consumption Rate ◽

Breast Cancer Cells ◽

Consumption Rate ◽

Oxygen Sensing

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A Peripheral CB1R Antagonist Increases Lipolysis, Oxygen Consumption Rate, and Markers of Beiging in 3T3-L1 Adipocytes Similar to RIM, Suggesting that Central Effects Can Be Avoided

International Journal of Molecular Sciences ◽

10.3390/ijms21186639 ◽

2020 ◽

Vol 21 (18) ◽

pp. 6639

Author(s):

Rebecca L. Paszkiewicz ◽

Richard N. Bergman ◽

Roberta S. Santos ◽

Aaron P. Frank ◽

Orison O. Woolcott ◽

...

Keyword(s):

Adipose Tissue ◽

Oxygen Consumption ◽

Oxygen Consumption Rate ◽

Consumption Rate ◽

Cannabinoid Receptor ◽

In Vitro Assays ◽

Mitochondrial Uncoupling ◽

Direct Role ◽

The Impact

With the increased prevalence of obesity and related co-morbidities, such as type 2 diabetes (T2D), worldwide, improvements in pharmacological treatments are necessary. The brain- and peripheral-cannabinoid receptor 1 (CB1R) antagonist rimonabant (RIM) has been shown to induce weight loss and improve glucose homeostasis. We have previously demonstrated that RIM promotes adipose tissue beiging and decreased adipocyte cell size, even during maintenance on a high-fat diet. Given the adverse side-effects of brain-penetrance with RIM, in this study we aimed to determine the site of action for a non-brain-penetrating CB1R antagonist AM6545. By using in vitro assays, we demonstrated the direct effects of this non-brain-penetrating CB1R antagonist on cultured adipocytes. Specifically, we showed, for the first time, that AM6545 significantly increases markers of adipose tissue beiging, mitochondrial biogenesis, and lipolysis in 3T3-L1 adipocytes. In addition, the oxygen consumption rate (OCR), consisting of baseline respiratory rate, proton leak, maximal respiratory capacity, and ATP synthase activity, was greater for cells exposed to AM6545, demonstrating greater mitochondrial uncoupling. Using a lipolysis inhibitor during real-time OCR measurements, we determined that the impact of CB1R antagonism on adipocytes is driven by increased lipolysis. Thus, our data suggest the direct role of CB1R antagonism on adipocytes does not require brain penetrance, supporting the importance of focus on peripheral CB1R antagonism pharmacology for reducing the incidence of obesity and T2D.

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Transiting to depth disrupts overall dynamic body acceleration and oxygen consumption rate in freely diving Steller sea lions

Marine Ecology Progress Series ◽

10.3354/meps11943 ◽

2016 ◽

Vol 562 ◽

pp. 221-236 ◽

Cited By ~ 3

Author(s):

BL Volpov ◽

ET Goundie ◽

DAS Rosen ◽

JPY Arnould ◽

AW Trites

Keyword(s):

Oxygen Consumption ◽

Oxygen Consumption Rate ◽

Consumption Rate ◽

Steller Sea Lions ◽

Body Acceleration ◽

Sea Lions ◽

Overall Dynamic Body Acceleration

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Microbial efficiency in a meromictic reservoir

Oceanological and Hydrobiological Studies ◽

10.2478/v10009-007-0047-9 ◽

2008 ◽

Vol 37 (2) ◽

Cited By ~ 2

Author(s):

Grażyna Mazurkiewicz-Boroń ◽

Teresa Bednarz ◽

Elżbieta Wilk-Woźniak

Keyword(s):

Carbon Dioxide ◽

Oxygen Consumption ◽

Release Rate ◽

Oxygen Consumption Rate ◽

Consumption Rate ◽

Metabolic Efficiency ◽

Sulphur Compounds ◽

Carbon Dioxide Release ◽

Microbial Efficiency ◽

Ion Contents

Microbial efficiency in a meromictic reservoirIndices of microbial efficiency (expressed as oxygen consumption and carbon dioxide release) were determined in the water column of the meromictic Piaseczno Reservoir (in an opencast sulphur mine), which is rich in sulphur compounds. Phytoplankton abundances were low in both the mixolimnion (up to 15 m depth) and monimolimnion (below 15 m depth). In summer and winter, carbon dioxide release was 3-fold and 5-fold higher, respectively, in the monimolimnion than in the mixolimnion. Laboratory enrichments of the sulphur substrate of the water resulted in a decrease in oxygen consumption rate of by about 42% in mixolimnion samples, and in the carbon dioxide release rate by about 69% in monimolimnion samples. Water temperature, pH and bivalent ion contents were of major importance in shaping the microbial metabolic efficiency in the mixolimnion, whilst in the monimolimnion these relationships were not evident.

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