scholarly journals Integrated proteomic and metabolomic analyses reveal the importance of aroma precursor accumulation and storage in methyl jasmonate-primed tea leaves

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jiang Shi ◽  
Jiatong Wang ◽  
Haipeng Lv ◽  
Qunhua Peng ◽  
Monika Schreiner ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Green Leaf Volatiles ◽  
Tea Plant ◽  
Plant Responses ◽  
Self Organizing Map ◽  
Tea Leaves ◽  
Leaf Quality ◽  
Geranyl Diphosphate ◽  
Leaf Volatiles ◽  
Novel Approach

AbstractIn response to preharvest priming with exogenous methyl jasmonate (MeJA), tea plants adjust their physiological behavior at the molecular level. The whole-organism reconfiguration of aroma formation from the precursor to storage is poorly understood. In this study, we performed iTRAQ proteomic analysis and identified 337, 246, and 413 differentially expressed proteins in tea leaves primed with MeJA for 12 h, 24 h, and 48 h, respectively. Furthermore, a total of 266 nonvolatile and 100 volatile differential metabolites were identified by utilizing MS-based metabolomics. A novel approach that incorporated the integration of extended self-organizing map-based dimensionality was applied. The vivid time-scale changes tracing physiological responses in MeJA-primed tea leaves are marked in these maps. Jasmonates responded quickly to the activation of the jasmonic acid pathway in tea leaves, while hydroxyl and glycosyl jasmonates were biosynthesized simultaneously on a massive scale to compensate for the exhausted defense. The levels of α-linolenic acid, geranyl diphosphate, farnesyl diphosphate, geranylgeranyl diphosphate, and phenylalanine, which are crucial aroma precursors, were found to be significantly changed in MeJA-primed tea leaves. Green leaf volatiles, volatile terpenoids, and volatile phenylpropanoids/benzenoids were spontaneously biosynthesized from responding precursors and subsequently converted to their corresponding glycosidic forms, which can be stably stored in tea leaves. This study elucidated the physiological response of tea leaves primed with exogenous methyl jasmonate and revealed the molecular basis of source and sink changes on tea aroma biosynthesis and catabolism in response to exogenous stimuli. The results significantly enhance our comprehensive understanding of tea plant responses to exogenous treatment and will lead to the development of promising biotechnologies to improve fresh tea leaf quality.

scholarly journals A comparative study of plant volatiles induced by insect and gastropod herbivory

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Leslie Mann ◽  
Diane Laplanche ◽  
Ted C. J. Turlings ◽  
Gaylord A. Desurmont
Keyword(s):  
Plant Species ◽  
Spodoptera Littoralis ◽  
Green Leaf Volatiles ◽  
Plant Responses ◽  
Insect Damage ◽  
Leaf Volatiles ◽  
Plant Volatile ◽  
Different Types ◽  
Cultivated Plant ◽  
Generalist Insect

AbstractInsect and gastropod herbivores are major plant consumers and their importance in the evolution of plant defensive traits is broadly recognized. However, their respective effects on plant responses have rarely been compared. Here we focused on plant volatile emissions (VOCs) following herbivory and compared the effects of herbivory by caterpillars of the generalist insect Spodoptera littoralis and by generalist slugs of the genus Arion on the VOCs emissions of 14 cultivated plant species. Results revealed that plants consistently produced higher amounts of volatiles and responded more specifically to caterpillar than to slug herbivory. Specifically, plants released on average 6.0 times more VOCs (total), 8.9 times more green leaf volatiles, 4.2 times more terpenoids, 6.0 times more aromatic hydrocarbons, and 5.7 times more other VOCs in response to 1 cm2 of insect damage than to 1 cm2 of slug damage. Interestingly, four of the plant species tested produced a distinct blend of volatiles following insect damage but not slug damage. These findings may result from different chemical elicitors or from physical differences in herbivory by the two herbivores. This study is an important step toward a more inclusive view of plant responses to different types of herbivores.

Green leaf volatiles enhance methyl jasmonate response in Arabidopsis

2012 ◽  
Vol 114 (5) ◽  
pp. 540-545 ◽  
Author(s):  
Toshihiro Hirao ◽  
Atsushi Okazawa ◽  
Kazuo Harada ◽  
Akio Kobayashi ◽  
Toshiya Muranaka ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Green Leaf Volatiles ◽  
Green Leaf ◽  
Leaf Volatiles

Review of recent advances on the production and eco-physiological roles of green leaf volatiles

2013 ◽  
Vol 37 (3) ◽  
pp. 268-275
Author(s):  
Hai-Feng SUN ◽  
Zhen-Yu LI ◽  
Bin WU ◽  
Xue-Mei QIN
Keyword(s):  
Green Leaf Volatiles ◽  
Green Leaf ◽  
Leaf Volatiles ◽  
Recent Advances

scholarly journals Optimizing the Production of Recombinant Hydroperoxide Lyase in Escherichia coli Using Statistical Design

Catalysts ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 176
Author(s):  
Sophie Vincenti ◽  
Magali Mariani ◽  
Jessica Croce ◽  
Eva Faillace ◽  
Virginie Brunini-Bronzini de Caraffa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Expression System ◽  
Scale Up ◽  
Food Additives ◽  
Statistical Design ◽  
Green Leaf Volatiles ◽  
Hydroperoxide Lyase ◽  
Soluble Enzyme ◽  
Leaf Volatiles ◽  
Conversion Rates

Hydroperoxide lyase (HPL) catalyzes the synthesis of volatiles C6 or C9 aldehydes from fatty acid hydroperoxides. These short carbon chain aldehydes, known as green leaf volatiles (GLV), are widely used in cosmetic industries and as food additives because of their “fresh green” aroma. To meet the growing demand for natural GLVs, the use of recombinant HPL as a biocatalyst in enzyme-catalyzed processes appears to be an interesting application. Previously, we cloned and expressed a 13-HPL from olive fruit in Escherichia coli and showed high conversion rates (up to 94%) during the synthesis of C6 aldehydes. To consider a scale-up of this process, optimization of the recombinant enzyme production is necessary. In this study, four host-vector combinations were tested. Experimental design and response surface methodology (RSM) were used to optimize the expression conditions. Three factors were considered, i.e., temperature, inducer concentration and induction duration. The Box–Behnken design consisted of 45 assays for each expression system performed in deep-well microplates. The regression models were built and fitted well to the experimental data (R2 coefficient > 97%). The best response (production level of the soluble enzyme) was obtained with E. coli BL21 DE3 cells. Using the optimal conditions, 2277 U L−1of culture of the soluble enzyme was produced in microliter plates and 21,920 U L−1of culture in an Erlenmeyer flask, which represents a 79-fold increase compared to the production levels previously reported.

scholarly journals Effects of Abiotic Factors on HIPV-Mediated Interactions between Plants and Parasitoids

2015 ◽  
Vol 2015 ◽  
pp. 1-18 ◽  
Author(s):  
Christine Becker ◽  
Nicolas Desneux ◽  
Lucie Monticelli ◽  
Xavier Fernandez ◽  
Thomas Michel ◽  
...  
Keyword(s):  
Plant Volatiles ◽  
Global Climate ◽  
Abiotic Factors ◽  
Biotic Interactions ◽  
Green Leaf Volatiles ◽  
Limited Attention ◽  
Emission Rates ◽  
Leaf Volatiles ◽  
Parasitic Hymenoptera ◽  
Growing Conditions

In contrast to constitutively emitted plant volatiles (PV), herbivore-induced plant volatiles (HIPV) are specifically emitted by plants when afflicted with herbivores. HIPV can be perceived by parasitoids and predators which parasitize or prey on the respective herbivores, including parasitic hymenoptera. HIPV act as signals and facilitate host/prey detection. They comprise a blend of compounds: main constituents are terpenoids and “green leaf volatiles.” Constitutive emission of PV is well known to be influenced by abiotic factors like temperature, light intensity, water, and nutrient availability. HIPV share biosynthetic pathways with constitutively emitted PV and might therefore likewise be affected by abiotic conditions. However, the effects of abiotic factors on HIPV-mediated biotic interactions have received only limited attention to date. HIPV being influenced by the plant’s growing conditions could have major implications for pest management. Quantitative and qualitative changes in HIPV blends may improve or impair biocontrol. Enhanced emission of HIPV may attract a larger number of natural enemies. Reduced emission rates or altered compositions, however, may render blends imperceptible to parasitoides and predators. Predicting the outcome of these changes is highly important for food production and for ecosystems affected by global climate change.

scholarly journals Verbenone and Green-Leaf Volatiles Reduce Whitebark Pine Mortality in a Northern Range-Expanding Mountain Pine Beetle Outbreak

2021 ◽  
Author(s):  
Etienne Cardinal ◽  
Brenda Shepherd ◽  
Jodie Krakowski ◽  
Carl James Schwarz ◽  
John Stirrett-Wood
Keyword(s):  
Mountain Pine Beetle ◽  
Green Leaf Volatiles ◽  
Green Leaf ◽  
Whitebark Pine ◽  
White Pine Blister Rust ◽  
Leaf Volatiles ◽  
Disease Resistant ◽  
Mountain Pine ◽  
Pine Beetle ◽  
Pine Trees

This is the first study testing effectiveness of semiochemical treatments to protect individual trees from a range-expanding mountain pine beetle (MPB, Dendroctonus ponderosae Hopkins) attack into newly exposed host populations of endangered whitebark pine (Pinus albicaulis Engelmann). We investigated the effectiveness of a combination of verbenone and Green-Leaf Volatiles (GLV) to protect rare and valuable disease-resistant trees during a MPB epidemic from 2015 to 2018 in Jasper National Park, Canada. Treatments reduced the proportion of trees attacked by MPB for all diameter classes, across all stands, from 46 to 60%. We also evaluated the effect of the exotic disease white pine blister rust (caused by the fungus Cronartium ribicola J.C. Fisch), the species’ other main regional threat. MPB were less likely to attack large, rust infected trees than healthy trees, emphasizing the value of the semiochemical treatment. Protecting large, cone-bearing disease-resistant whitebark pine trees is fundamental to whitebark pine recovery. Maintaining reproductive trees on the landscape increases the frequency and diversity of rust-resistant genotypes more effectively than just planting seedlings to replace MPB-killed trees, because this slow-growing species takes over 80 years to reproduce. Our study confirmed protecting large rust-resistant trees with verbenone and GLV is a proactive and effective treatment against MPB for whitebark pine in naïve populations.

scholarly journals Response of female Cydia molesta (Lepidoptera: Tortricidae) to plant derived volatiles

2003 ◽  
Vol 93 (4) ◽  
pp. 335-342 ◽  
Author(s):  
D. Natale ◽  
L. Mattiacci ◽  
A. Hern ◽  
E. Pasqualini ◽  
S. Dorn
Keyword(s):  
Host Plant ◽  
Green Leaf Volatiles ◽  
Oriental Fruit Moth ◽  
Gas Chromatography Mass Spectrometry ◽  
Headspace Volatiles ◽  
Component Mixture ◽  
Leaf Volatiles ◽  
Cydia Molesta ◽  
Bioactive Fraction ◽  
A Chain

AbstractPeach shoot volatiles were attractive to mated female oriental fruit moth, Cydia molesta (Busck), in a dual choice arena. No preference was observed between leaf odours from the principle host plant, peach, and the secondary host plant, apple. Twenty-two compounds were identified in headspace volatiles of peach shoots using gas chromatography–mass spectrometry. Green leaf volatiles accounted for more than 50% of the total emitted volatiles. A bioassay-assisted fractionation using different sorbent polymers indicated an attractant effect of compounds with a chain length of 6–8 carbon atoms. The major compounds of this fraction were tested either singly or in combinations for behavioural response of females. Significant bioactivity was found for a three-component mixture of (Z)-3-hexen-1-yl acetate, (Z)-3-hexen-1-ol and benzaldehyde in a 4:1:1 ratio. This synthetic mixture elicited a similar attractant effect as the full natural blend from peach shoots as well as the bioactive fraction.

Electroanalytical studies on green leaf volatiles for potential sensor development

The Analyst ◽  
2012 ◽  
Vol 137 (13) ◽  
pp. 3138 ◽  
Author(s):  
Yogeswaran Umasankar ◽  
Glen C. Rains ◽  
Ramaraja P. Ramasamy
Keyword(s):  
Green Leaf Volatiles ◽  
Green Leaf ◽  
Leaf Volatiles ◽  
Sensor Development
Sign in / Sign up

Export Citation Format

Share Document