DNA nano-pocket for ultra-selective uranyl extraction from seawater

Abstract Extraction of uranium from seawater is critical for the sustainable development of nuclear energy. However, the currently available uranium adsorbents are hampered by co-existing metal ion interference. DNAzymes exhibit high selectivity to specific metal ions, yet there is no DNA-based adsorbent for extraction of soluble minerals from seawater. Herein, the uranyl-binding DNA strand from the DNAzyme is polymerized into DNA-based uranium extraction hydrogel (DNA-UEH) that exhibits a high uranium adsorption capacity of 6.06 mg g−1 with 18.95 times high selectivity for uranium against vanadium in natural seawater. The uranium is found to be bound by oxygen atoms from the phosphate groups and the carbonyl groups, which formed the specific nano-pocket that empowers DNA-UEH with high selectivity and high binding affinity. This study both provides an adsorbent for uranium extraction from seawater and broadens the application of DNA for being used in recovery of high-value soluble minerals from seawater.

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Regulation of Activator/Dissociation Transposition by Replication and DNA Methylation

Genetics ◽

10.1093/genetics/157.4.1723 ◽

2001 ◽

Vol 157 (4) ◽

pp. 1723-1733

Author(s):

Francesca Ros ◽

Reinhard Kunze

Keyword(s):

Dna Methylation ◽

Transposable Elements ◽

Strong Evidence ◽

Complementary Strand ◽

Regulatory Effect ◽

Ds Excision ◽

Dna Strand ◽

Petunia Protoplasts ◽

Ac Transposase ◽

High Binding Affinity

Abstract In maize the transposable elements Activator/Dissociation (Ac/Ds) transpose shortly after replication from one of the two resulting chromatids (“chromatid selectivity”). A model has been suggested that explains this phenomenon as a consequence of different affinity for Ac transposase binding to holo-, hemi-, and unmethylated transposon ends. Here we demonstrate that in petunia cells a holomethylated Ds is unable to excise from a nonreplicating vector and that replication restores excision. A Ds element hemi-methylated on one DNA strand transposes in the absence of replication, whereas hemi-methylation of the complementary strand causes a >6.3-fold inhibition of Ds excision. Consistently in the active hemi-methylated state, the Ds ends have a high binding affinity for the transposase, whereas binding to inactive ends is strongly reduced. These results provide strong evidence for the above-mentioned model. Moreover, in the absence of DNA methylation, replication enhances Ds transposition in petunia protoplasts >8-fold and promotes formation of a predominant excision footprint. Accordingly, replication also has a methylation-independent regulatory effect on transposition.

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Photoinduced Multiple Effects to Enhance Uranium Extraction from Natural Seawater by Black Phosphorus Nanosheets

Angewandte Chemie ◽

10.1002/ange.201913644 ◽

2019 ◽

Vol 132 (3) ◽

pp. 1236-1243 ◽

Cited By ~ 7

Author(s):

Yihui Yuan ◽

Biye Niu ◽

Qiuhan Yu ◽

Xin Guo ◽

Zhanhu Guo ◽

...

Keyword(s):

Black Phosphorus ◽

Natural Seawater ◽

Uranium Extraction

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Highly Selective Separation and Enrichment of Phosphopeptides by Uranyl-Salophen Immobilized Silica Gel Material

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1095.341 ◽

2015 ◽

Vol 1095 ◽

pp. 341-344 ◽

Cited By ~ 1

Author(s):

Can Hui Xu ◽

Guang Liang Zhang ◽

Xin Zhou ◽

Xi Lin Xiao ◽

Chang Ming Nie ◽

...

Keyword(s):

Silica Gel ◽

Metal Ion ◽

High Selectivity ◽

Separation Efficiency ◽

Solid Phase ◽

Tetradentate Ligand ◽

Peptide Mixtures ◽

New Material ◽

Solid Phase Material

The characterization of phosphoproteins requires highly specific methods for the separation and enrichment of phosphopeptides. Here we report a novel metal ion-immobilized solid phase material for the separation and enrichment of phosphopeptides. The material is uranyl-salophen-silica gel (USSG) particles in which salophen is a tetradentate ligand of uranyl ion. In USSG salophen is connected on the surface of silica gel and uranyl is bound on the surface through its coordination with salophen. Phosphopeptides can be selectively retained by USSG because uranyl-salophen can bind phosphate moiety with strong affinity and high selectivity. The new material USSG has been successfully used for the separation of phosphopeptides from peptide mixtures with the separation efficiency of 97.0% to 97.4%.

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Engineering robust metal–phenolic network membranes for uranium extraction from seawater

Energy & Environmental Science ◽

10.1039/c8ee01438h ◽

2019 ◽

Vol 12 (2) ◽

pp. 607-614 ◽

Cited By ~ 71

Author(s):

Wei Luo ◽

Gao Xiao ◽

Fan Tian ◽

Joseph J. Richardson ◽

Yaping Wang ◽

...

Keyword(s):

East China Sea ◽

East China ◽

Natural Seawater ◽

The East China Sea ◽

Uranium Extraction ◽

China Sea

Biomass-derived membrane used for uranium extraction of natural seawater from the East China Sea through the formation of metal–phenolic networks.

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Synthesis of CuxNi(1−x)O coral-like nanostructures and their application in the design of a reusable toxic heavy metal ion sensor based on an adsorption-mediated electrochemical technique

Environmental Science Nano ◽

10.1039/c6en00285d ◽

2017 ◽

Vol 4 (1) ◽

pp. 191-202 ◽

Cited By ~ 7

Author(s):

Sayan Dey ◽

Sumita Santra ◽

Anupam Midya ◽

Prasanta Kumar Guha ◽

Samit Kumar Ray

Keyword(s):

Heavy Metal ◽

Detection Limit ◽

Metal Ion ◽

High Selectivity ◽

Heavy Metal Ion ◽

Electrochemical Technique ◽

Toxic Heavy Metal ◽

Ion Sensors ◽

Metal Ion Sensors ◽

Very High

Nanostructured, Cu-doped nickel oxides serve as excellent, ultra-fast, re-usable heavy metal ion sensors with an ultra-low detection limit and very high selectivity towards toxic Cr(vi) ions.

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Histone carbonylation in vivo and in vitro

Biochemical Journal ◽

10.1042/bj3510769 ◽

2000 ◽

Vol 351 (3) ◽

pp. 769-777 ◽

Cited By ~ 38

Author(s):

Georg T. WONDRAK ◽

Daniel CERVANTES-LAUREAN ◽

Elaine L. JACOBSON ◽

Myron K. JACOBSON

Keyword(s):

Histone H1 ◽

Nuclear Proteins ◽

Carbonyl Groups ◽

Cell Nuclei ◽

Strand Breaks ◽

Core Histones ◽

Dna Strand ◽

And Function

Non-enzymic damage to nuclear proteins has potentially severe consequences for the maintenance of genomic integrity. Introduction of carbonyl groups into histones in vivo and in vitro was assessed by Western blot immunoassay and reductive incorporation of tritium from radiolabelled NaBH4 (sodium borohydride). Histone H1 extracted from bovine thymus, liver and spleen was found to contain significantly elevated amounts of protein-bound carbonyl groups as compared with core histones. The carbonyl content of nuclear proteins of rat pheochromocytoma cells (PC12 cells) was not greatly increased following oxidative stress induced by H2O2, but was significantly increased following alkylating stress induced by N-methyl-N´-nitro-N-nitrosoguanidine or by combined oxidative and alkylating stress. Free ADP-ribose, a reducing sugar generated in the nucleus in proportion to DNA strand breaks, was shown to be a potent histone H1 carbonylating agent in isolated PC12 cell nuclei. Studies of the mechanism of histone H1 modification by ADP-ribose indicate that carbonylation involves formation of a stable acyclic ketoamine. Our results demonstrate preferential histone H1 carbonylation in vivo, with potentially important consequences for chromatin structure and function.

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Photoinduced Multiple Effects to Enhance Uranium Extraction from Natural Seawater by Black Phosphorus Nanosheets

Angewandte Chemie International Edition ◽

10.1002/anie.201913644 ◽

2019 ◽

Vol 59 (3) ◽

pp. 1220-1227 ◽

Cited By ~ 8

Author(s):

Yihui Yuan ◽

Biye Niu ◽

Qiuhan Yu ◽

Xin Guo ◽

Zhanhu Guo ◽

...

Keyword(s):

Black Phosphorus ◽

Natural Seawater ◽

Uranium Extraction

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Crystal structure of d(CCGGGGTACCCCGG)2at 1.4 Å resolution

Acta Crystallographica Section F Structural Biology Communications ◽

10.1107/s2053230x17004770 ◽

2017 ◽

Vol 73 (5) ◽

pp. 259-265

Author(s):

Selvam Karthik ◽

Arunachalam Thirugnanasambandam ◽

Pradeep Kumar Mandal ◽

Namasivayam Gautham

Keyword(s):

Crystal Structure ◽

Metal Ion ◽

Double Helix ◽

Barium Chloride ◽

Base Pairs ◽

X Ray ◽

Density Map ◽

Solvent Molecules ◽

Electron Density Map ◽

Phosphate Groups

The X-ray crystal structure of the DNA tetradecamer sequence d(CCGGGGTACCCCGG)2is reported at 1.4 Å resolution in the tetragonal space groupP41212. The sequence was designed to fold as a four-way junction. However, it forms an A-type double helix in the presence of barium chloride. The metal ion could not be identified in the electron-density map. The crystallographic asymmetric unit consists of one A-type double helix with 12 base pairs per turn, in contrast to 11 base pairs per turn for canonical A-DNA. A large number of solvent molecules have been identified in both the grooves of the duplex and around the backbone phosphate groups.

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