3D electron-beam writing at sub-15 nm resolution using spider silk as a resist

AbstractElectron beam lithography (EBL) is renowned to provide fabrication resolution in the deep nanometer scale. One major limitation of current EBL techniques is their incapability of arbitrary 3d nanofabrication. Resolution, structure integrity and functionalization are among the most important factors. Here we report all-aqueous-based, high-fidelity manufacturing of functional, arbitrary 3d nanostructures at a resolution of sub-15 nm using our developed voltage-regulated 3d EBL. Creating arbitrary 3d structures of high resolution and high strength at nanoscale is enabled by genetically engineering recombinant spider silk proteins as the resist. The ability to quantitatively define structural transitions with energetic electrons at different depths within the 3d protein matrix enables polymorphic spider silk proteins to be shaped approaching the molecular level. Furthermore, genetic or mesoscopic modification of spider silk proteins provides the opportunity to embed and stabilize physiochemical and/or biological functions within as-fabricated 3d nanostructures. Our approach empowers the rapid and flexible fabrication of heterogeneously functionalized and hierarchically structured 3d nanocomponents and nanodevices, offering opportunities in biomimetics, therapeutic devices and nanoscale robotics.

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3d Electron Printing in Recombinant Spider Silk Proteins at the Molecular Level

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII) ◽

10.1109/transducers.2019.8808515 ◽

2019 ◽

Author(s):

Nan Qin ◽

Zhiheng Gao ◽

Yu Zhou ◽

Tiger H. Tao

Keyword(s):

Spider Silk ◽

Molecular Level ◽

Silk Proteins ◽

3D Electron

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Biomimetic Spinning of Recombinant Silk Proteins

MRS Proceedings ◽

10.1557/proc-1239-vv07-20 ◽

2009 ◽

Vol 1239 ◽

Author(s):

David Keerl ◽

John George Hardy ◽

Thomas Scheibel

Keyword(s):

Spider Silk ◽

Molecular Level ◽

Genetically Engineered ◽

Primary Sequence ◽

Silk Proteins ◽

Fiber Production ◽

The Past ◽

Technical Realization ◽

Araneus Diadematus ◽

Biomimetic Approach

AbstractIn the past, we have successfully designed and produced a variety of engineered spider silk-like proteins (eADF3 and eADF4) based upon the primary sequence of the natural dragline proteins ADF3 and ADF4 from the spider Araneus diadematus [1]. Genetically engineered spider silk proteins can be modified at the molecular level to optimize the biochemical and mechanical properties of the final product. Although engineered spider silk proteins can be processed into fibers using different spinning methods, our group is interested in the technical realization of a biomimetic approach. Here, we present an overview over our biomimetic fiber production process.

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Nanoscale Self-Assembly Using Ion and Electron Beam Techniques: A Rapid Review

MRS Advances ◽

10.1557/adv.2020.349 ◽

2020 ◽

Vol 5 (64) ◽

pp. 3507-3520

Author(s):

Chunhui Dai ◽

Kriti Agarwal ◽

Jeong-Hyun Cho

Keyword(s):

Electron Beam ◽

Self Assembly ◽

Ion Beam ◽

Three Dimensional ◽

The Self ◽

Stress Generation ◽

Rapid Review ◽

High Yield ◽

3D Nanostructures ◽

Self Assembled

AbstractNanoscale self-assembly, as a technique to transform two-dimensional (2D) planar patterns into three-dimensional (3D) nanoscale architectures, has achieved tremendous success in the past decade. However, an assembly process at nanoscale is easily affected by small unavoidable variations in sample conditions and reaction environment, resulting in a low yield. Recently, in-situ monitored self-assembly based on ion and electron irradiation has stood out as a promising candidate to overcome this limitation. The usage of ion and electron beam allows stress generation and real-time observation simultaneously, which significantly enhances the controllability of self-assembly. This enables the realization of various complex 3D nanostructures with a high yield. The additional dimension of the self-assembled 3D nanostructures opens the possibility to explore novel properties that cannot be demonstrated in 2D planar patterns. Here, we present a rapid review on the recent achievements and challenges in nanoscale self-assembly using electron and ion beam techniques, followed by a discussion of the novel optical properties achieved in the self-assembled 3D nanostructures.

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Designing of spider silk proteins for hiPSC-based cardiac tissue engineering

Materials Today Bio ◽

10.1016/j.mtbio.2021.100114 ◽

2021 ◽

pp. 100114

Author(s):

Tilman U. Esser ◽

Vanessa T. Trossmann ◽

Sarah Lentz ◽

Felix B. Engel ◽

Thomas Scheibel

Keyword(s):

Tissue Engineering ◽

Spider Silk ◽

Cardiac Tissue ◽

Cardiac Tissue Engineering ◽

Silk Proteins

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A Molecular‐Level Interface Design Enabled High‐Strength and High‐Toughness Carbon Nanotube Buckypaper

Macromolecular Materials and Engineering ◽

10.1002/mame.202100244 ◽

2021 ◽

pp. 2100244

Author(s):

Lulu Shen ◽

Yushun Zhao ◽

Peter Samora Owuor ◽

Chao Wang ◽

Chao Sui ◽

...

Keyword(s):

Carbon Nanotube ◽

Interface Design ◽

Molecular Level ◽

High Strength ◽

High Toughness

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Advancing conjugated polymers into nanometer-scale devices

Pure and Applied Chemistry ◽

10.1351/pac200678101803 ◽

2006 ◽

Vol 78 (10) ◽

pp. 1803-1822 ◽

Cited By ~ 7

Author(s):

Wenping Hu ◽

Hiroshi Nakashima ◽

Erjing Wang ◽

Kazuaki Furukawa ◽

Hongxiang Li ◽

...

Keyword(s):

Conjugated Polymers ◽

Molecular Level ◽

Nanometer Scale ◽

Transport Mechanisms ◽

Phenylene Ethynylene ◽

Polymer Molecules ◽

Polymer Electronics ◽

End Groups

In this article, we review the possibility of combining conjugated polymers with nanometer-scale devices (nanodevices), in order to introduce the properties associated with conjugated polymers into such nanodevices. This approach envisages combining the highly topical disciplines of polymer electronics and nanoelectronics to engender a new subdirection of polymer nanoelectronics, which can serve as a tool to probe the behavior of polymer molecules at the nanometer/molecular level, and contribute to clarifying transport mechanisms in conjugated polymers. In this study, we exemplify this combination, using a family of linear and conjugated polymers, poly(p-phenylene-ethynylene)s (PPEs) with thiolacetate-functionalized end groups.

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