The non-invasive exfoliated transcriptome (exfoliome) reflects the tissue-level transcriptome in a mouse model of NSAID enteropathy

Abstract Background: Q fever is a worldwide zoonosis caused by Coxiella burnetii and mainly transmitted by aerosols. This study aims at establishing a systematic and efficient mouse model of acute Q fever via intratracheal (IT) inoculation of aerosolized C. burnetii. Methods: BALB/c mice were infected with C. burnetii via IT route using a non-invasive aerosol pulmonary delivery device to directly place the living C. burnetii organisms into their tracheas. The bacterial loads, pathological lesions, and serological responses were analyzed in mice, and compared with those of mice infected via intraperitoneal (IP) route. Results: As early as at day three post-infection (pi) with a low dose of C. burnetii (1×10⁴ per mouse), a large amount of C. burnetii organisms were determined in blood, lungs, hearts, livers, and spleens of the mice. The inflammatory infiltration was observed in hearts and lungs of mice. Compared with mice infected via IP route, the mice infected via IT route exhibited a higher level of bacterial loads and more severe pathological lesions in hearts and lungs at day 3 and day 7 pi. Conclusions: These data indicated that IT route is more efficient than IP route to cause acute C. burnetii infection in mice. Overall, we successfully established a mouse model of C. burnetii infection via IT route, which is useful for investigations of pathogenesis and immunity of acute C. burnetii infection as well as evaluation of therapeutic drugs and preventive vaccines of Q fever.

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Non-invasive diffusion tensor imaging detects white matter degeneration in the spinal cord of a mouse model of amyotrophic lateral sclerosis

NeuroImage ◽

10.1016/j.neuroimage.2010.12.044 ◽

2011 ◽

Vol 55 (2) ◽

pp. 455-461 ◽

Cited By ~ 34

Author(s):

Clare K. Underwood ◽

Nyoman D. Kurniawan ◽

Tim J. Butler ◽

Gary J. Cowin ◽

Robyn H. Wallace

Keyword(s):

Spinal Cord ◽

Amyotrophic Lateral Sclerosis ◽

Diffusion Tensor Imaging ◽

White Matter ◽

Mouse Model ◽

Diffusion Tensor ◽

Non Invasive ◽

White Matter Degeneration ◽

Lateral Sclerosis

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Anaemia and Haemoglobin Status: A New Concept and a New Method of Assessment

Food and Nutrition Bulletin ◽

10.1177/156482659601700106 ◽

1996 ◽

Vol 17 (1) ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Rainer Gross ◽

Marcus Gliwitzki ◽

Patrick Gross ◽

Klaus Frank

Keyword(s):

Oxygen Supply ◽

Haemoglobin Concentration ◽

Tissue Level ◽

Intervention Strategies ◽

Blood Oxygenation ◽

The Body ◽

Non Invasive ◽

Method Of Assessment ◽

The Impact ◽

Tissue Oxygen Supply

Traditionally, anaemia has been determined and interpreted by the magnitude and severity of iron deficiency and the impact of intervention strategies. Internationally, it is defined as a state in which the quality and/or quantity of circulating red cells are reduced below a normal level The body employs several mechanisms during the development of anaemia to maintain the oxygen supply to the tissues. Thus, applying any quantitative cut-off point as an indicator for anaemia may lead to misclassification, since haemoglobin concentration does not necessarily reflect the level of tissue oxygen supply. Ideally, an assessment strategy should be able to determine both the degree of haemoglobin oxygenation and the haemoglobin concentration at a tissue level. The Erlangen microlight-guide spectrophotometer is a non-invasive instrument that can assess both capillary blood oxygenation and relative haemoglobin concentration.

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Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer’s Disease in 5XFAD Mouse Model

Frontiers in Genetics ◽

10.3389/fgene.2020.565479 ◽

2020 ◽

Vol 11 ◽

Cited By ~ 1

Author(s):

Zhiqi Song ◽

Yanfeng Xu ◽

Ling Zhang ◽

Li Zhou ◽

Yu Zhang ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Mouse Model ◽

Proteomic Profiling ◽

Non Invasive ◽

Early Biomarkers ◽

Urinary Exosomes ◽

5Xfad Mouse

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Cervical epithelial damage promotes Ureaplasma parvum ascending infection, intrauterine inflammation and preterm birth induction in mice

Nature Communications ◽

10.1038/s41467-019-14089-y ◽

2020 ◽

Vol 11 (1) ◽

Cited By ~ 7

Author(s):

Ioannis Pavlidis ◽

Owen B. Spiller ◽

Gabriella Sammut Demarco ◽

Heather MacPherson ◽

Sarah E. M. Howie ◽

...

Keyword(s):

Preterm Birth ◽

Mouse Model ◽

Bacterial Load ◽

Preterm Births ◽

Intrauterine Infection ◽

Cervical Injury ◽

Epithelial Damage ◽

Ureaplasma Parvum ◽

Non Invasive ◽

Ascending Infection

AbstractAround 40% of preterm births are attributed to ascending intrauterine infection, and Ureaplasma parvum (UP) is commonly isolated in these cases. Here we present a mouse model of ascending UP infection that resembles human disease, using vaginal inoculation combined with mild cervical injury induced by a common spermicide (Nonoxynol-9, as a surrogate for any mechanism of cervical epithelial damage). We measure bacterial load in a non-invasive manner using a luciferase-expressing UP strain, and post-mortem by qPCR and bacterial titration. Cervical exposure to Nonoxynol-9, 24 h pre-inoculation, facilitates intrauterine UP infection, upregulates pro-inflammatory cytokines, and increases preterm birth rates from 13 to 28%. Our results highlight the crucial role of the cervical epithelium as a barrier against ascending infection. In addition, we expect the mouse model will facilitate further research on the potential links between UP infection and preterm birth.

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