scholarly journals Insights into the skin microbiome dynamics of leprosy patients during multi-drug therapy and in healthy individuals from Brazil

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Paulo E. S. Silva ◽  
Mariana P. Reis ◽  
Marcelo P. Ávila ◽  
Marcela F. Dias ◽  
Patrícia S. Costa ◽  
...  
Keyword(s):  
Drug Therapy ◽  
Skin Microbiome ◽  
Healthy Individuals ◽  
Leprosy Patients

scholarly journals The First Report of Diversity Analyses of Skin Microbiome In Indonesian Leprosy Patient

2020 ◽  
Author(s):  
Hendra Gunawan ◽  
Pati Aji Achdiat ◽  
Oki Suwarsa ◽  
Dhaifina Alkatirie ◽  
Endang Sutedja ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Normal Skin ◽  
Alpha Diversity ◽  
Skin Lesions ◽  
Protective Role ◽  
Leprosy Patient ◽  
Skin Microbiome ◽  
Healthy Individuals ◽  
Microbiome Profile ◽  
Leprosy Patients

Abstract BackgroundSkin microbiome is quiet diverse. There are several factors influencing the skin microbiome, such as skin diseases. However, the effects of leprosy on the skin microbiome remain unclear and there are only a few studies about skin microbiome on leprosy. The aim of this study was to investigate the alpha diversity of skin microbiome on lesional site of multibacillary (MB) leprosy patients who visited the top referral hospital in West Java Indonesia. Here in this study we characterize the skin microbiome in leprosy patient in compared to healthy individual by using next generation 16S rRNA sequencing. A total 18 skin swab samples were collected from 18 samples (14 leprosy patients, 4 healthy individuals).ResultsTaxonomic analysis of leprous skin lesions revealed main five phyla: Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes, and Cyanobacteria. Proteobacteria and Firmicutes were overrepresented in leprosy patients, while Actinobacteria, Bacteroidetes, and Cyanobacteria were diminished in leprosy patients compared to healthy individuals. The main five genera in leprous skin lesions were Staphylococcus, Acinetobacter, Corynebacterium, Micrococcus, and Propionibacterium. Staphylococcus, Acinetobacter, and Micrococcus were enriched in leprosy patients, while Corynebacterium and Propionibacterium which have a protective role in normal skin, were diminished in leprosy patients when compared with healthy individuals. Twenty-five species were found in leprous skin lesions that were not typical in human skin and considered as potentially pathogenic. The alpha diversity analysis showed that leprous skin lesions is less diverse than that of the healthy skin microbiome.ConclusionAs a conclusion, the skin microbiome on lesional site of leprosy patient show alteration and less diverse compare to healthy individuals. This suggest that leprosy can affects skin microbiome profile or otherwise.

S275 – Nasal Morphology and Nasal Patency in Leprosy Patients

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P167-P167
Author(s):  
Maria Teresa Torres Larrosa ◽  
Luis Jorge Peréz Peréz ◽  
Juan-Jose Artazkoz-del Toro
Keyword(s):  
Drug Therapy ◽  
Therapy Group ◽  
Control Group ◽  
Nasal Patency ◽  
Multidrug Therapy ◽  
Airway Patency ◽  
Expiratory Resistance ◽  
Leprosy Patients ◽  
The Right ◽  
The Impact

Objectives 1) To assess the impact of multi-drug leprosy therapy on the development of nasal deformities and nasal airway patency. 2) Evaluate the nasal morphology and nasal patency in leprosy patients treated with the multidrug therapy in comparison with patients treated with 1 drug therapy and a group of healthy volunteers. Methods In an overall group of 84 patients studied, 38 were treated with a therapy based on a single drug, and 22 were treated with multi-drug therapy, while 24 subjects formed a control group. We used anterior rhinoscopy to analised the morphology of the nose. We meassured the nasal inspiratory and expiratory resistance of the right and left nostrils and total nasal inspiratory and expiratory resistance at a transnasal pressure of 150 Pa. by using active anterior rhinomanometry. The statistical analysis was carried out using the Varianza analisys. Results The nasal structures in the 1-drug therapy group underwent bone and cartilaginous resorption with an increase in nasal resistances. We found significant statistical differences between the resistance values obtained in this group and the control group (p<0,05). In the multidrug therapy group, the morphology of the nose remains as in healthy patients. No significant statistical differences were found between the resistance values obtained in the multidrug therapy patients and the control group (P>0,05). Conclusions The multidrug therapy prevents developing nasal deformities and maintains a normal nasal airflow.

scholarly journals Significance of Histopathology in Leprosy Patients with 1–5 Skin Lesions with Relevance to Therapy

2011 ◽  
Vol 3 (01) ◽  
pp. 021-024 ◽  
Author(s):  
S Veena ◽  
Prakash Kumar ◽  
Shashikala P. ◽  
Gurubasavaraj H. ◽  
H R Chandrasekhar ◽  
...  
Keyword(s):  
Drug Therapy ◽  
Skin Lesion ◽  
Skin Lesions ◽  
Clinicopathological Correlation ◽  
Adequate Treatment ◽  
Duration Of Therapy ◽  
Tuberculoid Leprosy ◽  
Leprosy Patients ◽  
Skin Biopsies ◽  
Severity Of The Disease

ABSTRACT Background: Patients with 1-5 skin lesions are clinically categorized as paucibacillary for treatment purposes. For betterment and adequate treatment of patients, this grouping needs further study. Aim: To study a group of leprosy patients with 1-5 skin lesions, compare clinical details with histopathological findings and bacteriological status of the skin to evaluate the relevance of this grouping. Materials and Methods: Two-year study involving 31 patients of leprosy with 1-5 skin lesions was included in this study. A number of skin lesions were recorded. Skin biopsies were taken in all patients. The biopsies were evaluated for the type of pathology and acid fast bacilli (AFB) status. Results: Of 31 patients, 19 (61.2%) had single skin lesion, 7 (22.5%) had two lesions, 4 (12.9%) had three lesions, and only one (3.22%) had four lesions, there were no patients with five lesions. Of the 31 patients, 30 (96.7%) were clinically diagnosed as borderline tuberculoid and one patient (3.22%) has tuberculoid leprosy. Skin smears were negative for AFB in all patients. The histological diagnoses were: TT 1 (3.22%), BT 24 (77.41%), and IL 6 (19.2%). AFB were found in 2 (6.45%) out of 31 skin biopsies. Clinicopathological correlation was 76.6% in the BT group. Conclusion: Tissue biopsy findings in 1-5 skin lesions which were not considered relevant for treatment purposes until now should be given a status in the categorization and assessment of severity of the disease. The significance of finding of AFB and histopathology of multibacillary (MB) type of leprosy in tissue biopsies, in patients grouped as PB should be resolved so that patients could be given the drug therapy and duration of therapy they warrant.

scholarly journals Nasal carriage of Mycobacterium leprae DNA in healthy individuals in Lega Robi village, Ethiopia

2003 ◽  
Vol 131 (2) ◽  
pp. 841-848 ◽  
Author(s):  
D. BEYENE ◽  
A. ASEFFA ◽  
M. HARBOE ◽  
D. KIDANE ◽  
M. MACDONALD ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Mycobacterium Leprae ◽  
Nasal Carriage ◽  
Elisa Test ◽  
Annual Number ◽  
Multiple Drug ◽  
Healthy Individuals ◽  
Leprosy Patients ◽  
Multiple Drug Therapy ◽  
Statistical Criteria

The number of registered leprosy patients world-wide has decreased dramatically after extensive application of WHO recommended Multiple Drug Therapy (MDT). The annual number of new cases has, however, been almost unchanged in several populations, indicating that the infection is still present at community level. Nasal carriage of Mycobacterium leprae DNA was studied in Lega Robi village in Ethiopia. MDT had been applied for more than ten years, and 718 residents over 5 years old were eligible for the study. During the first survey nasal swab samples were collected from 664 (92·5%) individuals. The results of a Peptide Nucleic Acid-ELISA test for M. leprae DNA interpreted by stringent statistical criteria were available for 589 (88·7%) subjects. Thirty-five (5·9%) individuals without clinical signs of leprosy were positive for M. leprae DNA. Seven PCR positive individuals lived in a household where one or two other members were also positive for M. leprae DNA. During a second survey 8 (4·6%) of 175 interpretable PNA-ELISA tests were positive. Of 137 individuals tested twice, only two were positive on both occasions whereas 10 were PCR positive only once. The study confirms the widespread distribution of M. leprae DNA in healthy individuals. The feasibility of curbing possible transmission of subclinical infection needs further consideration.

scholarly journals 16S rDNA based skin microbiome data of healthy individuals and leprosy patients from India

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Nitin Bayal ◽  
Sunil Nagpal ◽  
Mohammed Monzoorul Haque ◽  
Milind S. Patole ◽  
Vijayalakshmi Valluri ◽  
...  
Keyword(s):  
16S Rdna ◽  
Peripheral Nerves ◽  
Skin Microbiome ◽  
Considerable Decrease ◽  
Rdna Sequencing ◽  
Leprosy Patients ◽  
Diseased State ◽  
Unaffected Skin ◽  
Microbiome Data ◽  
Geographical Locations

Abstract Leprosy is an infectious disease that has predilection in skin and peripheral nerves. Skin has its own microbiome, however it is not extensively studied in Indian leprosy patients. Here, by using next-generation 16S rDNA sequencing, we have attempted to assess the skin associated microbial diversity pertaining to affected and unaffected skin of Indian leprosy patients. A total of 90 skin swab samples were collected from 60 individuals (30 healthy controls, 30 patients) residing in Hyderabad and Miraj, two distinct geographical locations in India to assess the homo/heterogeneity of skin microbial signatures. While a large increase in genus Methylobacterium and Pseudomonas was seen in patients from Miraj and Hyderabad respectively, a considerable decrease in genus Staphylococcus in the leprosy patients (as compared to controls) from both geographical locations was also observed. We expect that, these datasets can not-only provide further interesting insights, but will also help to observe dynamics of microbiome in the diseased state and generate hypotheses to test for skin microbiome transplantation studies in leprosy.

scholarly journals Detection of Mycobacterium leprae DNA for 36kDa protein in urine from leprosy patients: a preliminary report

2004 ◽  
Vol 46 (5) ◽  
pp. 275-277 ◽  
Author(s):  
Om Parkash ◽  
Hari Bhan Singh ◽  
Subha Rai ◽  
Archna Pandey ◽  
Vishwa Mohan Katoch ◽  
...  
Keyword(s):  
Treatment Response ◽  
Preliminary Report ◽  
Mycobacterium Leprae ◽  
Lepromatous Leprosy ◽  
Healthy Individuals ◽  
Specific Pcr ◽  
Number Of Patients ◽  
Tuberculoid Leprosy ◽  
Leprosy Patients ◽  
First Time

We have searched for Mycobacterium leprae DNA for 36kDa protein in urine using a M. leprae specific PCR technique. A limited number of 16 patients (of which 11 belonged to lepromatous leprosy and five to tuberculoid leprosy) and eight healthy individuals were included for the present study. The number of urine samples positive by PCR were 36.4% (4/11) in lepromatous patients and 40% (2/5) in tuberculoid patients. None of the samples from healthy individuals was positive. To our knowledge, the results indicate, for the first time, the presence of M. leprae DNA in urine from leprosy patients. Another important finding obtained out of the study is that amongst treated patients 66.6% (4/6) were positive whereas amongst untreated only 20% (2/10) were positive. From the present indicative data it appears that treatment improves the PCR results with urine as a sample. Thus, the approach could prove to be useful for monitoring the treatment response of individual patients and needs to be further evaluated with a large number of patients.

Mycobacterial-induced cytotoxic T cells as well as nonspecific killer cells derived from healthy individuals and leprosy patients

1990 ◽  
Vol 20 (12) ◽  
pp. 2651-2659 ◽  
Author(s):  
Birhane Kaleab ◽  
Tom Ottenoff ◽  
Paul Converse ◽  
Eva Halapi ◽  
Genet Tadesse ◽  
...  
Keyword(s):  
T Cells ◽  
Cytotoxic T Cells ◽  
Healthy Individuals ◽  
Killer Cells ◽  
Leprosy Patients
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