scholarly journals Formaldehyde quantification using ampicillin is not selective

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Raphael Reinbold ◽  
Tobias John ◽  
Paolo Spingardi ◽  
Akane Kawamura ◽  
Amber L. Thompson ◽  
...  
Keyword(s):  
Biological Samples ◽  
Carbonyl Compounds ◽  
Detection Method ◽  
Spectroscopic Studies ◽  
Fluorescence Properties ◽  
Limiting Factor ◽  
Selective Detection ◽  
Biologically Relevant ◽  
Human Metabolite ◽  
Detection And Quantification

AbstractFormaldehyde (HCHO) is a simple and highly reactive human metabolite but its biochemistry is poorly defined. A limiting factor in HCHO research is lack of validated quantification methods for HCHO relevant to biological samples. We describe spectroscopic studies on a reported fluorescence-based HCHO detection method involving its reaction with ampicillin. The results validate the structure and fluorescence properties of the HCHO-ampicillin reaction product. However, the same adduct is observed after reaction of ampicillin with glyoxylate. Related fluorophores were formed with other biologically relevant carbonyl compounds. Overall, our studies suggest the ampicillin method is not reliable for selective detection and quantification of HCHO in biological samples.

Electronically-tuned triarylmethine scaffolds for fast and continuous monitoring of H2S levels in biological samples

The Analyst ◽  
2019 ◽  
Vol 144 (14) ◽  
pp. 4210-4218
Author(s):  
Ramshad Kalluruttimmal ◽  
Divya Thekke Thattariyil ◽  
Archana Panthalattu Parambil ◽  
Ashis Kumar Sen ◽  
Lakshmi Chakkumkumarath ◽  
...  
Keyword(s):  
Spectrophotometric Method ◽  
Biological Samples ◽  
Continuous Monitoring ◽  
Selective Detection ◽  
Detection And Quantification

A fast and reliable spectrophotometric method for the selective detection and quantification of H2S in biological samples is presented.

13C NMR spectroscopic studies of the behaviors of carbonyl compounds in various solutions

2017 ◽  
Vol 58 (50) ◽  
pp. 4677-4681 ◽  
Author(s):  
Yoshikazu Hiraga ◽  
Saori Chaki ◽  
Satomi Niwayama
Keyword(s):  
13C Nmr ◽  
Carbonyl Compounds ◽  
Spectroscopic Studies

A label-free biosensor for selective detection of DNA and Pb2+ based on a G-quadruplex

RSC Advances ◽  
2016 ◽  
Vol 6 (10) ◽  
pp. 7765-7771 ◽  
Author(s):  
Ruipeng Hou ◽  
Xiaoqing Niu ◽  
Fengling Cui
Keyword(s):  
Detection Method ◽  
Selective Detection ◽  
Label Free ◽  
Schematic Representation ◽  
G Quadruplex

Schematic representation of the DNA and Pb2+ detection method.

scholarly journals Terbium-Tetracarboxylate Framework as a Luminescent Probe for the Selective Detection of Nitrofurazone

Crystals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 222
Author(s):  
Qipeng Li ◽  
Yanqiong Shen ◽  
Junsong Zhao ◽  
Zejun Zhang ◽  
Jinjie Qian
Keyword(s):  
Hydrogen Bond ◽  
Layered Structure ◽  
Sensitive Detection ◽  
Fluorescence Properties ◽  
Selective Detection ◽  
Supramolecular Framework ◽  
Luminescent Probe ◽  
Hydrogen Bond Interactions ◽  
Secondary Building Units

A novel terbium-tetracarboxylate framework with the 5,5’-(diazene-1,2-iyl)diisophthalic acid (H4abtc) ligand, formulated as [Tb(Habtc)(DMSO)(H2O)2]n (ZTU-5), has been synthesized and structurally characterized. ZTU-5 features a 2D-layered structure constructed by the binuclear terbium secondary building units (SBUs) and abtc4– ligand, which can be further expanded into a 3D-supramolecular framework by the hydrogen bond interactions. In addition, the magnetic and fluorescence properties of ZTU-5 are investigated and ZTU-5 exhibits highly selective and sensitive detection of nitrofurazone (NZF).

Investigation of silicon doping into carbon dots for improved fluorescence properties for selective detection of Fe3+ ion

2019 ◽  
Vol 96 ◽  
pp. 109374 ◽  
Author(s):  
Mittal L. Desai ◽  
Hirakendu Basu ◽  
Sudeshna Saha ◽  
Rakesh Kumar Singhal ◽  
Suresh Kumar Kailasa
Keyword(s):  
Carbon Dots ◽  
Fluorescence Properties ◽  
Selective Detection ◽  
Silicon Doping

scholarly journals Capacity of Nine Thermostable DNA Polymerases To Mediate DNA Amplification in the Presence of PCR-Inhibiting Samples

1998 ◽  
Vol 64 (10) ◽  
pp. 3748-3753 ◽  
Author(s):  
Waleed Abu Al-Soud ◽  
Peter Rådström
Keyword(s):  
Dna Polymerase ◽  
Biological Samples ◽  
Detection Method ◽  
Dna Polymerases ◽  
Dna Amplification ◽  
Nucleic Acid Amplification ◽  
Full Potential ◽  
Thermus Aquaticus ◽  
Pcr Inhibitors ◽  
Thermostable Dna Polymerase

ABSTRACT The PCR is an extremely powerful method for detecting microorganisms. However, its full potential as a rapid detection method is limited by the inhibition of the thermostable DNA polymerase fromThermus aquaticus by many components found in complex biological samples. In this study, we have compared the effects of known PCR-inhibiting samples on nine thermostable DNA polymerases. Samples of blood, cheese, feces, and meat, as well as various ions, were added to PCR mixtures containing various thermostable DNA polymerases. The nucleic acid amplification capacity of the nine polymerases, under buffer conditions recommended by the manufacturers, was evaluated by using a PCR-based detection method for Listeria monocytogenes in the presence of purified template DNA and different concentrations of PCR inhibitors. The AmpliTaqGold and the Taq DNA polymerases from Thermus aquaticus were totally inhibited in the presence of 0.004% (vol/vol) blood in the PCR mixture, while the HotTub, Pwo, rTth, andTfl DNA polymerases were able to amplify DNA in the presence of 20% (vol/vol) blood without reduced amplification sensitivity. The DNA polymerase from Thermotoga maritima(Ultma) was found to be the most susceptible to PCR inhibitors present in cheese, feces, and meat samples. When the inhibitory effect of K and Na ions was tested on the nine polymerases, HotTub from Thermus flavus and rTthfrom Thermus thermophilus were the most resistant. Thus, the PCR-inhibiting effect of various components in biological samples can, to some extent, be eliminated by the use of the appropriate thermostable DNA polymerase.

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