scholarly journals Rapid antibiotic susceptibility testing of bacteria from patients’ blood via assaying bacterial metabolic response with surface-enhanced Raman spectroscopy

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yin-Yi Han ◽  
Yi-Chun Lin ◽  
Wei-Chih Cheng ◽  
Yu-Tzu Lin ◽  
Lee-Jene Teng ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Metabolic Response ◽  
Surface Enhanced Raman Spectroscopy ◽  
Antibiotic Susceptibility Testing ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Rapid identification and quantification of the antibiotic susceptibility of lactic acid bacteria using surface enhanced Raman spectroscopy

2020 ◽  
Vol 12 (3) ◽  
pp. 376-382 ◽  
Author(s):  
Panxue Wang ◽  
Xuejie Wang ◽  
Yan Sun ◽  
Guoli Gong ◽  
Mingtao Fan ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Antibiotic Susceptibility ◽  
Early Period ◽  
Surface Enhanced Raman Spectroscopy ◽  
Rapid Identification ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Identification And Quantification

SERS was used to discriminate and quantify the antibiotic susceptibility of lactic acid bacteria at the early period of treatment.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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