Nanofibrous asymmetric collagen/curcumin membrane containing aspirin-loaded PLGA nanoparticles for guided bone regeneration

Abstract The goal of the current study was to develop an asymmetric guided bone regeneration (GBR) membrane benefiting from curcumin and aspirin. The membrane was prepared using electrospinning technique and then was physic-chemically characterized by the conventional methods. The release profile of aspirin from the prepared membrane was also measured by ultraviolet spectrophotometry. Also, the antibacterial activities of the membrane was evaluated. We also assessed the in vitro effects of the prepared membrane on the biocompatibility and osteogenic differentiation of dental pulp stem cells (DPSCs), and evaluated in vivo bone regeneration using the prepared membrane in the defects created in both sides of the dog’s jaw by histology. The results from the characterization specified that the membrane was successfully prepared with monodispersed nanosized fibers, uniform network shaped morphology, negative surface charge and sustained release platform for aspirin. The membrane also showed antimicrobial effects against all tested bacteria. The presence of curcumin and aspirin in the asymmetric membrane enhanced osteogenic potential at both transcriptional and translational levels. The results of the animal test showed that the test area was completely filled with new bone after just 28 days, while the commercial membrane area remained empty. There was also a soft tissue layer above the new bone area in the test side. We suggested that the prepared membrane in this work could be used as a GBR membrane to keep soft tissue from occupying bone defects in GBR surgeries. Besides, the surgeries can be benefited from antibacterial activities and bone healing effects of this novel GBR membrane while, simultaneously, promoting bone regeneration.

Download Full-text

Zn-Containing Membranes for Guided Bone Regeneration in Dentistry

Polymers ◽

10.3390/polym13111797 ◽

2021 ◽

Vol 13 (11) ◽

pp. 1797

Author(s):

Manuel Toledano ◽

Marta Vallecillo-Rivas ◽

María T. Osorio ◽

Esther Muñoz-Soto ◽

Manuel Toledano-Osorio ◽

...

Keyword(s):

Mechanical Properties ◽

Bone Regeneration ◽

Bone Healing ◽

Bone Repair ◽

Complex Modulus ◽

Bacterial Colonization ◽

Guided Bone Regeneration ◽

M2 Macrophage

Barrier membranes are employed in guided bone regeneration (GBR) to facilitate bone in-growth. A bioactive and biomimetic Zn-doped membrane with the ability to participate in bone healing and regeneration is necessary. The aim of the present study is to state the effect of doping the membranes for GBR with zinc compounds in the improvement of bone regeneration. A literature search was conducted using electronic databases, such as PubMed, MEDLINE, DIMDI, Embase, Scopus and Web of Science. A narrative exploratory review was undertaken, focusing on the antibacterial effects, physicochemical and biological properties of Zn-loaded membranes. Bioactivity, bone formation and cytotoxicity were analyzed. Microstructure and mechanical properties of these membranes were also determined. Zn-doped membranes have inhibited in vivo and in vitro bacterial colonization. Zn-alloy and Zn-doped membranes attained good biocompatibility and were found to be non-toxic to cells. The Zn-doped matrices showed feasible mechanical properties, such as flexibility, strength, complex modulus and tan delta. Zn incorporation in polymeric membranes provided the highest regenerative efficiency for bone healing in experimental animals, potentiating osteogenesis, angiogenesis, biological activity and a balanced remodeling. Zn-loaded membranes doped with SiO2 nanoparticles have performed as bioactive modulators provoking an M2 macrophage increase and are a potential biomaterial for promoting bone repair. Zn-doped membranes have promoted pro-healing phenotypes.

Download Full-text

Antibacterial Properties of Guided Bone Regeneration Membrane for Periodontal Applications

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.606.47 ◽

2014 ◽

Vol 606 ◽

pp. 47-50

Author(s):

Nur Najiha Saarani ◽

K. Jamuna-Thevi ◽

Ida Idayu Muhammad ◽

Hendra Hermawan

Keyword(s):

Staphylococcus Aureus ◽

Bone Regeneration ◽

Composite Membrane ◽

Lauric Acid ◽

Antibacterial Properties ◽

Guided Bone Regeneration ◽

Significant Inhibition ◽

Thermally Induced ◽

Zones Of Inhibition ◽

Gbr Membrane

Guided Bone Regeneration (GBR) membrane is used as a barrier to prevent soft tissue ingrowth and to encourage bone regeneration through cellular exclusion. This study aims to assess antibacterial properties of recently developed three-layered poly(lactic-co-glycolic acid) (PLGA) /lauric acid (LA) composite membrane towards Staphylococcus aureus. One of the outmost layers of three-layered membrane was incorporated with 1-3 wt% of LA. The composite membrane was developed using thermally induced phase separation/solvent leaching technique. SEM results shows formation of PLGA matrix with smaller pores by the addition of 1 wt% LA compared with pure PLGA membrane. Samples of 1.7 cm diameter disk containing 1, 2 and 3 wt% of lauric acid were tested and pure membrane without lauric acid was used as a control. Results showed that the zones of inhibition were 2.3 cm and 2.5 cm for the 2 wt% and 3 wt% LA-containing membranes, respectively. However, 1 wt% LA-containing membrane observed to have no inhibition at all, indicating that increasing concentration of LA has significant inhibition against Staphylococcus aureus. The 3 wt% LA composition will be used in the mechanically optimized membranes for degradation studies in future works.

Download Full-text

Superficial Topography and Porosity of an Absorbable Barrier Membrane Impacts Soft Tissue Response in Guided Bone Regeneration

Journal of Periodontology ◽

10.1902/jop.2010.090592 ◽

2010 ◽

Vol 81 (6) ◽

pp. 926-933 ◽

Cited By ~ 16

Author(s):

Ronaldo Barcellos de Santana ◽

Carolina Miller Leite de Mattos ◽

Carlos Eduardo Francischone ◽

Thomas Van Dyke

Keyword(s):

Soft Tissue ◽

Bone Regeneration ◽

Guided Bone Regeneration ◽

Tissue Response ◽

Barrier Membrane ◽

Soft Tissue Response

Download Full-text

In Vitro and In Vivo Evaluation of a nHA/PA66 Composite Membrane for Guided Bone Regeneration

Journal of Biomaterials Science Polymer Edition ◽

10.1163/092050609x12602753096279 ◽

2011 ◽

Vol 22 (1-3) ◽

pp. 263-275 ◽

Cited By ~ 15

Author(s):

Jidong Li ◽

Yi Man ◽

Yi Zuo ◽

Li Zhang ◽

Cui Huang ◽

...

Keyword(s):

Bone Regeneration ◽

Composite Membrane ◽

Guided Bone Regeneration ◽

In Vivo Evaluation

Download Full-text

In Vitro Physico-Chemical Characterization and Standardized In Vivo Evaluation of Biocompatibility of a New Synthetic Membrane for Guided Bone Regeneration

Materials ◽

10.3390/ma12071186 ◽

2019 ◽

Vol 12 (7) ◽

pp. 1186

Author(s):

Lívia da Costa Pereira ◽

Carlos Fernando de Almeida Barros Mourão ◽

Adriana Terezinha Neves Novellino Alves ◽

Rodrigo Figueiredo de Brito Resende ◽

Marcelo José Pinheiro Guedes de Uzeda ◽

...

Keyword(s):

Bone Regeneration ◽

Chemical Properties ◽

Chemical Characterization ◽

Guided Bone Regeneration ◽

Tissue Reaction ◽

In Vivo Evaluation ◽

Physico Chemical ◽

Tissue Reactions

This study’s aim was to evaluate the biocompatibility and bioabsorption of a new membrane for guided bone regeneration (polylactic-co-glycolic acid associated with hydroxyapatite and β-tricalcium phosphate) with three thicknesses (200, 500, and 700 µm) implanted in mice subcutaneously. Scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and the quantification of carbon, hydrogen and nitrogen were used to characterize the physico-chemical properties. One hundred Balb-C mice were divided into 5 experimental groups: Group 1—Sham (without implantation); Group 2—200 μm; Group 3—500 μm; Group 4—700 μm; and Group 5—Pratix®. Each group was subdivided into four experimental periods (7, 30, 60 and 90 days). Samples were collected and processed for histological and histomorphometrical evaluation. The membranes showed no moderate or severe tissue reactions during the experimental periods studied. The 500-μm membrane showed no tissue reaction during any experimental period. The 200-μm membrane began to exhibit fragmentation after 30 days, while the 500-μm and 700-µm membranes began fragmentation at 90 days. All membranes studied were biocompatible and the 500 µm membrane showed the best results for absorption and tissue reaction, indicating its potential for clinical guided bone regeneration.

Download Full-text