scholarly journals Occurrence and risks of antibiotics in an urban river in northeastern Tibetan Plateau

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yuzhu Kuang ◽  
Xiaoyu Guo ◽  
Jingrun Hu ◽  
Si Li ◽  
Ruijie Zhang ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Tibetan Plateau ◽  
River Water ◽  
Sewage Treatment ◽  
Antibiotic Resistance Genes ◽  
Aquatic Organisms ◽  
Urban River ◽  
Resistance Evolution ◽  
Northeastern Tibetan Plateau ◽  
Plateau Area

AbstractThere is a dearth of information on the occurrence and risks of antibiotics in the urban rivers from plateau areas. This study investigated 83 antibiotics in water and sediments of an urban river and effluents of sewage treatment plants (E-STPs) in Xining, Qinghai (northeastern Tibetan Plateau). Fifty-three antibiotics were detected, and the concentrations of individual antibiotics varied in the range of undetected (ND)-552 ng/L in water, ND-164 ng/g in sediments, and ND-3821 ng/L in E-STPs. Seasonal differences of antibiotic concentrations were significant for water samples (p < 0.05) but insignificant for sediments (p > 0.05). In urban area, E-STP is the main source of antibiotics in the river, while runoff from manured cropland contributes partially to antibiotics in the river in the suburban area. The antibiotic compositions in water were different from those in sediments, but were similar to those in E-STPs. Notably, because of strong solar radiation and long sunshine hours in the plateau area, low levels of quinolones, which are sensitive to photolysis, were observed in river water. Moreover, norfloxacin and enrofloxacin, observed in urban river from other regions of China, were not detected in the Huangshui River water. The occurrence of ofloxacin, erythromycin, roxithromycin, clarithromycin, and trimethoprim in E-STPs may induce a possible risk to antibiotic resistance evolution. Trimethoprim, anhydroerythromycin, sulfamethoxazole, sulfapyridine, and clindamycin in river water could pose low to medium risks to aquatic organisms. Further investigation on the occurrence and distribution of antibiotic resistance genes in the Huangshui River is urgently needed.

Concentration of facultative pathogenic bacteria and antibiotic resistance genes during sewage treatment and in receiving rivers

2016 ◽  
Vol 74 (8) ◽  
pp. 1753-1763 ◽  
Author(s):  
Stefanie Heß ◽  
Frauke Lüddeke ◽  
Claudia Gallert
Keyword(s):  
Antibiotic Resistance ◽  
River Water ◽  
Resistance Genes ◽  
Pathogenic Bacteria ◽  
Quantitative Polymerase Chain Reaction ◽  
Molecular Genetic ◽  
Sewage Treatment ◽  
Antibiotic Resistance Genes ◽  
Qpcr Data ◽  
Total Bacteria

Whereas the hygienic condition of drinking and bathing water by law must be monitored by culture-based methods, for quantification of microbes and antibiotic resistance in soil or the aquatic environment, often molecular genetic assays are used. For comparison of both methods, knowledge of their correlation is necessary. Therefore the population of total bacteria, Escherichia coli, enterococci and staphylococci during sewage treatment and in receiving river water was compared by agar plating and quantitative polymerase chain reaction (qPCR) assays. In parallel, all samples were investigated for clinically relevant antibiotic resistance genes. Whereas plating and qPCR data for total bacteria correlated well in sewage after primary treatment, qPCR data of river water indicated higher cell numbers for E. coli. It is unknown if these cells are ‘only’ not growing under standard conditions or if they are dead. Corresponding to the amount of non-culturable cells, the ‘breakpoints’ for monitoring water quality should be adapted. The abundances of clinically relevant antibiotic resistance genes in river water were in the same order of magnitude or even higher than in treated sewage. For estimation of the health risk it is important to investigate which species carry respective genes and whether these genes are disseminated via gene transfer.

scholarly journals Complete Genome Sequence ofPhotobacterium damselaeSubsp.damselaeStrain SSPD1601 Isolated from Deep-Sea Cage-CulturedSebastes schlegeliiwith Septic Skin Ulcer

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Yongxiang Yu ◽  
Zheng Zhang ◽  
Yingeng Wang ◽  
Meijie Liao ◽  
Xiaojun Rong ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Deep Sea ◽  
Clinical Symptoms ◽  
Antibiotic Resistance Genes ◽  
Aquatic Organisms ◽  
Skin Ulcer ◽  
Rrna Genes ◽  
Trna Genes ◽  
Photobacterium Damselae ◽  
Sea Cage

Photobacterium damselaesubsp.damselae(PDD) is a Gram-negative bacterium that can infect a variety of aquatic organisms and humans. Based on an epidemiological investigation conducted over the past 3 years, PDD is one of the most important pathogens causing septic skin ulcer in deep-sea cage-culturedSebastes schlegeliiin the Huang-Bohai Sea area and present throughout the year with high abundance. To further understand the pathogenicity of this species, the pathogenic properties and genome of PDD strain SSPD1601 were analyzed. The results revealed that PDD strain SSPD1601 is a rod-shaped cell with a single polar flagellum, and the clinical symptoms were replicated during artificial infection. The SSPD1601 genome consists of two chromosomes and two plasmids, totaling 4,252,294 bp with 3,751 coding sequences (CDSs), 196 tRNA genes, and 47 rRNA genes. Common virulence factors including flagellin,Fur,RstB,hcpA, OMPs,htpB-Hsp60,VasK, andvgrGwere found in strain SSPD1601. Furthermore, SSPD1601 is a pPHDD1-negative strain containing the hemolysin genehlyAchand three putative hemolysins (emrA, yoaF, and VPA0226), which are likely responsible for the pathogenicity of SSPD1601. The phylogenetic analysis revealed SSPD1601 to be most closely related to Phdp Wu-1. In addition, the antibiotic resistance phenotype indicated that SSPD1601 was not sensitive to ceftazidime, pipemidic, streptomycin, cefalexin, bacitracin, cefoperazone sodium, acetylspiramycin, clarithromycin, amikacin, gentamycin, kanamycin, oxacillin, ampicillin, and trimethoprim-sulfamethoxazole, but only the bacitracin resistance genebacAwas detected based on Antibiotic Resistance Genes Database. These results expand our understanding of PDD, setting the stage for further studies of its pathogenesis and disease prevention.

scholarly journals Monitoring and Evaluation of Antibiotic Resistance Genes in Three Rivers in Northeast China

2021 ◽  
Author(s):  
Chen Zhao ◽  
Chenyu Li ◽  
Xiaoming Wang ◽  
Zhuosong Cao ◽  
Chao Gao ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
River Water ◽  
Resistance Genes ◽  
Northeast China ◽  
Pathogenic Bacteria ◽  
Respiratory Infections ◽  
Antibiotic Resistance Genes ◽  
Distribution Characteristics ◽  
Beta Lactam ◽  
Three Rivers

Abstract Background: Antibiotic resistance genes (ARGs) have become an important public health problem. In this study, we used metagenomic sequencing to analyze the composition of ARGs in certain original habitats of northeast China, comprising three different rivers and riverbank soils of the Heilongjiang River, Tumen River, and Yalu River. Results: Twenty types of ARG were detected in every water sample. The major ARGs were multidrug resistance genes, at approximately 0.5 copies/16s rRNA, accounting for 57.5% of the total ARG abundance. The abundance of multidrug, bacitracin, beta-lactam, macrolide‑lincosamide‑streptogramin, sulfonamide, fosmidomycin, and polymyxin resistance genes covered 96.9% of the total ARG abundance. No significant ecological boundary of ARG diversity was observed. The compositions of the resistance genes in the three rivers were very similar to each other, and 92.1% of ARG subtypes were shared by all water samples. Except for vancomycin resistance genes, almost all ARGs in riverbank soils were detected in the river water. About 31.05% ARGs were carried by Pseudomonas. Opportunistic pathogenic bacteria carrying resistance genes were mainly related to diarrhea and respiratory infections. Multidrug and beta-lactam resistance genes correlated positively with mobile genetic elements (MGEs), indicating a potential risk of diffusion.Conclusions: The composition of ARGs in three different rivers was similar, indicating that climate played an important role in ARG occurrence. ARG subtypes in river water were almost completely the same as those in riverbank soil. ARGs had no significant geographical distribution characteristics. Many ARGs were carried by human pathogenic bacteria related to human diarrhea and respiratory infections, such as Pseudomonas aeruginosa and Aeromonas caviae. In general, our results provide a valuable dataset of river water ARG distribution in northeast China. The related ecological geography distribution characteristics should be further explored.

scholarly journals The Occurrence of Antibiotic Resistance Genes in an Urban River in Nepal

Water ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 450 ◽  
Author(s):  
Ocean Thakali ◽  
Sarmila Tandukar ◽  
John Brooks ◽  
Samendra Sherchan ◽  
Jeevan Sherchand ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Anthropogenic Activities ◽  
Quantitative Polymerase Chain Reaction ◽  
Antibiotic Resistance Genes ◽  
Urban River ◽  
Class 1 Integron ◽  
Upstream Site ◽  
Class 1 ◽  
The Impact

Urban rivers affected by anthropogenic activities can act as reservoirs of antibiotic resistance genes (ARGs). This study aimed to describe the occurrence of selected ARGs (blaTEM, ermF, mecA, and tetA) and a class 1 integron (intI1) in an urban river in Nepal. A total of 18 water samples were collected periodically from upstream, midstream, and downstream sites along the Bagmati River over a 1-year period. All ARGs except mecA and intI1 were consistently detected by a quantitative polymerase chain reaction in the midstream and downstream sites, with concentrations ranging from 3.1 to 7.8 log copies/mL. ARG abundance was significantly lower at the upstream site (p < 0.05), reflecting the impact of anthropogenic activities on increasing concentrations of ARGs at midstream and downstream sites. Our findings demonstrate the presence of clinically relevant ARGs in the urban river water of Nepal, suggesting a need for mitigating strategies to prevent the spread of antibiotic resistance in the environment.

Temporal variation and sharing of antibiotic resistance genes between water and wild fish gut in a peri-urban river

2021 ◽  
Vol 103 ◽  
pp. 12-19
Author(s):  
Zhen-Chao Zhou ◽  
Ze-Jun Lin ◽  
Xin-Yi Shuai ◽  
Ji Zheng ◽  
Ling-Xuan Meng ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Temporal Variation ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Urban River ◽  
Wild Fish ◽  
Fish Gut

scholarly journals Antibiotic Resistance Characteristics of Environmental Bacteria from an Oxytetracycline Production Wastewater Treatment Plant and the Receiving River

2010 ◽  
Vol 76 (11) ◽  
pp. 3444-3451 ◽  
Author(s):  
Dong Li ◽  
Tao Yu ◽  
Yu Zhang ◽  
Min Yang ◽  
Zhen Li ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
River Water ◽  
Resistance Genes ◽  
Treatment Plant ◽  
Antibiotic Resistance Genes ◽  
Tetracycline Resistance ◽  
Production Plant ◽  
Bacterial Strains ◽  
High Concentration ◽  
Receiving River

ABSTRACT We characterized the bacterial populations in surface water receiving effluent from an oxytetracycline (OTC) production plant. Additional sampling sites included the receiving river water 5 km upstream and 20 km downstream from the discharge point. High levels of OTC were found in the wastewater (WW), and the antibiotic was still detectable in river water downstream (RWD), with undetectable levels in river water upstream (RWU). A total of 341 bacterial strains were isolated using nonselective media, with the majority being identified as Gammaproteobacteria. The MICs were determined for 10 antibiotics representing seven different classes of antibiotics, and the corresponding values were significantly higher for the WW and RWD isolates than for the RWU isolates. Almost all bacteria (97%) from the WW and RWD samples demonstrated multidrug-resistant (MDR) phenotypes, while in RWU samples, these were less frequent (28%). The WW and RWD isolates were analyzed for the presence of 23 tetracycline (tet) resistance genes. The majority of isolates (94.2% and 95.4% in WW and RWD, respectively) harbored the corresponding genes, with tet(A) being the most common (67.0%), followed by tet(W), tet(C), tet(J), tet(L), tet(D), tet(Y), and tet(K) (in the range between 21.0% and 40.6%). Class I integrons were detected in the majority of WW and RWD isolates (97.4% and 86.2%, respectively) but were not associated with the tet genes. We hypothesize that the strong selective pressure imposed by a high concentration of OTC contributes to the wide dissemination of tetracycline resistance genes and other antibiotic resistance genes, possibly through mobile genetic elements.

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