A Pilot Study on Deworming Wild Foxes for Echinococcus spp. in Qinghai, China

Background: Wild foxes play an important role in echinococcosis epidemics. There have been a few studies investigating Echinococcus spp. in the Qinghai-Tibet Plateau area, China, but none on the prevention and control of Echinococcus spp. in wild foxes. Methods: From 2016 March through December 2019, two wild fox dens were selected as two test sites based on prior long-term camera screening and observation. Anthelmintic praziquantel tablets were placed near the two dens of wild foxes, and the wild foxes freely consumed the anthelmintic drugs. Morphological methods were used to detect initially the parasite species, and PCR molecular methods were used to identify accurately parasite and host species. Results: Parasite eggs of E. multilocularis (2/11, 18.2%) were found in 11 fecal samples. Importantly, the eggs of E. multilocularis (1/21, 4.8%) were found again in the feces of the foxes one year later; moreover, the eggs of E. multilocularis (2/19, 10.5%) still existed in the feces of the foxes two years later. Conclusion: Wild foxes were repeatedly infected with E. multilocularis and that deworming for prevention and control is required at least twice per year. Prevention and control methods for echinococcosis in wild foxes were explored, providing a scientific basis for the prevention and control of echinococcosis in wild animals.

Ptyctimous mites (Acari: Acariformes: Oribatida) as zooindicators of changes in Dorrigo National Park in Australia (North Coast Bioregion)

The authors of the study present an analysis of the structure and changes in the examined community of ptyctimous mites (Acari: Acariformes: Oribatida) found in Dorrigo National Park in Australia. The research was conducted during two periods: between 1990 and 1993 and later in 2007. The analysed mite community comprises 35 species, though, the dominance and frequency of particular species were different for each period. In the first research period (1990–1993) in the area of Dorrigo National Park, 28 species were recorded, whereas in 2007 – 23 species were found. There were 16 species that occurred in both research periods, and 12 species only in the samples collected in the 90’s, and 7 species only in those collected in 2007. The analysis also embraces the geographical distribution of the species in the area of Australia. Three species were designated as endemic, occurring only in the area of the examined national park (Austrophthiracarus dissonus Niedbała et Collof, 1997, Austrophthiracarus parapulchellus Niedbała, 2006 and Notophthiracarus distinctus Niedbała, 1989). The analysed samples contained only few specimens of these species. Due to the low abundance, great rarity of the local populations and high endemism, these species should be regarded as potentially endangered (EN according to the IUCN scale). A comparative analysis of the community from Dorrigo National Park (New South Wales) with those found in other larger areas of Australia in Victoria (Otway Ranges Area, Yarra Ranges Area, Strzelecki Ranges Area and Errinundra Plateau Area) examined by Niedbała and Szywilewska-Szczykutowicz (2017) has revealed that the communities found in Dorrigo National Park contained far more species, which constituted 30% of the whole fauna of Australia. In contrast to the communities of ptyctimous mites from Dorrigo, the individual communities in the area of Victoria contained only between 5% and 14% of all known species in Australia from this group.

The alleles of AGT and HIF1A gene affect the risk of hypertension in plateau residents

Plateau essential hypertension is a common chronic harmful disease of permanent residents in plateau areas. Studies have shown some single nucleotide polymorphisms (SNPs) associations with hypertension, but few have been verified in plateau area-lived people. In this paper, we examined some hypertension-related gene loci to analyze the relationship between risk SNPs and plateau essential hypertension in residents in Qinghai-Tibet plateau area. We screened hypertension-related SNPs from the literature, Clinvar database, GHR database, GTR database, and GWAS database, and then selected 101 susceptible SNPs for detection. Illumina MiSeq NGS platform was used to perform DNA sequencing on the blood samples from 185 Tibetan dwellings of Qinghai, and bioinformatic tools were used to make genotyping. Genetic models adjusted by gender and age were used to calculate the risk effects of genotypes. Four known SNPs as well as a new locus were found associated with PHE, which were rs2493134 (AGT), rs9349379 (PHACTR1), rs1371182 (CYP2C56P-PRPS1P1), rs567481079 (CYP2C56P-PRPS1P1), and chr14:61734822 (HIF1A). Among them, genotypes of rs2493134, rs9349379, and rs567481079 were risk factors, genotypes of rs1371182 and chr14:61734822 were protective factors. The rs2493134 in AGT was found associated with an increased risk of the plateau essential hypertension by 3.24-, 3.24-, and 2.06-fold in co-dominant, dominant, and Log-additive models, respectively. The rs9349379 in PHACTR1 is associated with a 2.61-fold increased risk of plateau essential hypertension according to the dominant model. This study reveals that the alleles of AGT, HIF1A, and PHACTR1 are closely related to plateau essential hypertension risk in the plateau Tibetan population.

Seasonal Variation in Chemical Compositions of Essential Oils Extracted from Lavandin Flowers in the Yun-Gui Plateau of China

Lavandin, as an important cash crop, is cultivated in Kunming, Yun-Gui Plateau of China. For the special growing environment, Lavandin was grown here and used to investigate the changes in the yield and chemical compositions of essential oils extracted from the flowers in different seasons. The essential oils were extracted by hydro-distillation and analysis by gas chromatography-mass spectrometry (GC-MS). Results indicated great changes in chemical composition depending on the season of harvesting. The yields of essential oils ranged from 2.0% to 3.8% among the seasons, and the highest yield was in the summer. Chemical composition data showed that the extracted oils were rich in oxygenated monoterpenes (55.4–81.4%), eucalyptol (38.7–49.8%), camphor (8.41–14.26%), α-bisabolol (6.6–25.5%), and linalool (4.6–12.5%). The contents of eucalyptol and α-bisabolol changed in a contrary trend with seasonal variations. The results provided new insight for Chinese Lavandin germplasm to be used in application and development, and reference to the researcher, the farmer, and investor for sustainable industrialization of the plant grown in the Yun-Gui Plateau of China, but also the similar plateau area of the sustainable developments.

PERAN DAN KOLABORASI STAKEHOLDER PADA PERKEMBANGAN PARIWISATA HERITAGE DI KAWASAN SIWA PLATEAU DAERAH ISTIMEWA YOGYAKARTA

This paper investigates variety of role and collaboration of stakeholders involved with heritage tourism development in Siwa Plateau Area, Special Region of Yogyakarta Province in order to better understand whether and how those roles and collaborations reflect an effort to find a balance related to inherent contradictions in the concepts of heritage (conservation/preservation) and tourism (change/development). The method used was qualitative inductive with primary data obtained by survey, interview and observation. The variety of role and collaboration forms of stakeholders reflects at least three main aspects with variety of nuance. First is heritage protection and development control. Second is being partners in managing heritage as a tourist attraction/destination. Third is that there are active efforts to involve local communities as key players in heritage tourism development in the region. Artikel ini menggali bentuk-bentuk peran dan kolaborasi stakeholder yang terlibat pada perkembangan pariwisata heritage di Kawasan Siwa Plaetau (KSP) Daerah Istimewa Yogyakarta, untuk lebih memahami apakah dan bagaimana peran dan kolaborasi yang berlangsung tersebut mencerminkan upaya-upaya mencari keseimbangan dalam kontradiksi inheren yang terdapat di dalam konsep heritage (preservasi/stabilitas) dan pariwisata (pembangunan/perubahan). Penelitian dilakukan secara induktif kualitatif dengan data-data utama diperoleh dari survei, wawancara dan observasi. Ragam bentuk peran dan kolaborasi tersebut mencerminkan setidaknya tiga aspek utama dengan beragam nuansa di dalamnya, yaitu pelindungan heritage dan kontrol pembangunan, kerjasama atau mitra dalam mengelola heritage sebagai objek wisata, dan upaya-upaya untuk melibatkan komunitas-komunitas lokal sebagai pemain kunci dalam perkembangan pariwisata heritage di dalam kawasan.