scholarly journals Removal of zinc(II) from livestock and poultry sewage by a zinc(II) resistant bacteria

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jiang Huang ◽  
Jihong Wang ◽  
Lan Jia

AbstractIn order to remediate Zn-contaminated livestock and poultry sewage, a zinc-resistant bacterial strain was screened and isolated from the manure of livestock and poultry and identified by molecular biology. The optimal conditions for removing zinc(II) from strain XZN4 were determined by single-factor experiments as follows: within 3 times of repeated use, pH value was 5, initial concentration of zinc(II) was 100 mg/L, the amount of bacteria was 6 g/L, the temperature was 25–30 °C, and the removal equilibrium time was 60 min. Then, through adsorption isotherm model, scanning electron microscope image, energy dispersive spectrum analysis, infrared spectrum analysis and sterilization control experiment, it was found that the removal of zinc(II) by bacteria was single-molecule layer adsorption, which was carried out in coordination with degradation. The influence of different concentrations of copper(II), ammonia nitrogen, phosphorus, and chlortetracycline on the removal of zinc(II) from livestock and poultry sewage by XZN4 strain in the actual application was discussed. The bacteria can reduce the concentration of zinc(II) from the complex livestock and poultry waste water to below the discharge standard, and has a strong environmental tolerance, the highest removal rate reached 88.6% and the highest removal amount reached 10.30 mg/L. The screening and application of XZN4 strain can thus be of great significance for the microbial treatment of zinc(II) in complex livestock and poultry sewage. The results will provide guidance for the microbial remediation of heavy metal pollution.

2014 ◽  
Vol 955-959 ◽  
pp. 360-365
Author(s):  
Min Han ◽  
Cheng Hong Feng ◽  
Shun Li Wang ◽  
Ye Quan Fu ◽  
Li Qing An ◽  
...  

To explore purification approach of anaerobic-digested-slurry from cattle dung, this study translated a technology in inorganic coagulation. Under the same condition, a coagulation experiment was carried out by three kinds inorganic coagulants such as polyaluminium chloride (PAC), aluminum sulfate and ferric chloride, respectively, then measured indicators of pH value, BOD5 value, CODcr value, ammonia nitrogen value, turbidity value. Results showed that there was a varying degree of purification effect, and the removal rate of the above indicators increased as the coagulant dosage increasing, the ferric chloride with the dosage of 960mg/L was the best one in purifying effect among the three coagulants. It is feasible that coagulation technology was used to pretreatment biogas slurry from cattle dung, and most of the indicators reached Chinese national standard basically (GB 18596-2001).


2012 ◽  
Vol 588-589 ◽  
pp. 55-58
Author(s):  
Yong Feng Li ◽  
Jian Yu Yang ◽  
Guo Cai Zhang

Simulate sewage were used in an anaerobic-anoxic-aerobic biological nutrient removal system(A2O process), by observing the pHs in different compartments and its reflected changes in nitrogen and phosphorus removal, studied on the effects of different pHs on the removal of pollutants. The experiments indicates that the anaerobic phosphorus release showed the main performance of the decline of pH, denitrification in anoxic zone caused the rise of pH, uptake of phosphate in the aerobic zone mainly caused the continuous rise of pH. There is no evidently changes in COD removal, ammonia nitrogen get the highest removal as the pH value was between 8.0-8.5, when pH was at 6.5-7.5, the TN have the maximum removal rate, TP can keep in a high level when the pH was above 6.0.


Author(s):  
Mengyuan Zou ◽  
Hongmin Dong ◽  
Zhiping Zhu ◽  
Yuanhang Zhan

Ammonia stripping is a pretreatment method for piggery biogas slurry, and the effectiveness of the method is affected by many factors. Based on the results of single-factor experiments, response surface methodology is adopted to establish a quadratic polynomial mathematical model relating stripping time, pH value and gas flow rate to the average removal rate of ammonia nitrogen to explore the interactions among various influencing factors, obtain optimized combined parameters for ammonia stripping, and carry out experimental verification of the parameters. The results show that when hollow polyhedral packing is adopted under operating conditions including a stripping time of 90 min, pH value of 11, gas flow rate of 28 m3/h, gas–liquid ratio of 2000 and temperature of 30 °C, the average removal rate of ammonia nitrogen in biogas slurry can reach approximately 73%. The experimental value is only 4.2% different from the predicted value, which indicates that analysis on the interaction among factors influencing ammonia stripping of biogas slurry and parameter optimization of the regression model are accurate and effective.


2015 ◽  
Vol 1092-1093 ◽  
pp. 1068-1072
Author(s):  
Yi Feng Lei ◽  
Li Na Zheng ◽  
Xin Ran Jiang ◽  
Wei Nan Wu ◽  
Yi Ming Han

By zeolite column adsorption test process of nitrate pollution of groundwater, the research of three different inlet velocity of nitrate nitrogen removal, and the roles of nitrite nitrogen accumulation, zeolite adsorption of ammonia nitrogen and the influence of pH value changes, the results show that when the flow rate of 70 mL/h, the nitrate nitrogen concentration in the reaction column rising velocity under concentration less than before, but nitrate nitrogen concentration in the zeolite column has remained at about 3 mg/L, removal rate has remained at more than 95%; Nitrite nitrogen accumulation showed a trend of gradual decline, within the scope of flow rate of 90 mL/h, not affected by the late response, nitrite nitrogen concentration of 0.2 mg/L; As the reaction progresses, pH value gradually rose slightly, but still within the range of 6.0 to 8.0; Flow rate of 50 mL/h, zeolite adsorption of ammonia nitrogen effect is best.


2012 ◽  
Vol 476-478 ◽  
pp. 2001-2004
Author(s):  
Xiao Cai Yu ◽  
Peng Fei Zhu ◽  
Kui Sheng Song ◽  
Dong Dong Hu ◽  
Qian Du

The Fe3+-doped nano-TiO2 catalyst with various amounts of dopant Fe3+ irons was prepared by a sol-gel method. The products were characterized by XRD and SEM. The photocatalytic degradation of ammonia nitrogen in aquaculture wastewater was investigated by using Fe3+-doped nano-TiO2 under UV irradiation. In the experiment, the effect of Fe3+/TiO2 dosage, the ratio of dopant Fe3+, ammonia-N initial concentration, pH value, H2O2 volume concentration, and reaction time, respectively, on the removal of ammonia-N was investigated. The experimental results can be stated as follows: when the ratio of dopant Fe3+ was 0.25% wt, the dosage of Fe3+/TiO2 was 0.7 g/L, the initial concentration of ammonia-N was 10 mg/L, H2O2 volume fraction was 4 %, respectively, if the reaction time may last 4 h, the removal rate of ammonia-N was expected to reach 97.17 %.


2011 ◽  
Vol 197-198 ◽  
pp. 774-779
Author(s):  
Peng Fei Zhu ◽  
Xiao Cai Yu ◽  
Qian Du ◽  
Kui Sheng Song ◽  
Zhong Hua He

The nano-TiO2photocatalyst was prepared via sol-gel method, and the crystal structure and surface morphology were characterized by XRD and SEM. The photocatalytic degradation of ammonia-N in aquaculture wastewater was investigated by using nano-TiO2under UV irradiation. In the experiment, the effect of nano-TiO2dosage, ammonia-N initial concentration, pH value, H2O2volume concentration, and reaction time, respectively, on the removal of ammonia-N was investigated. On the basis of the results of these experimental data, an orthogonal array experimental design was used to select more efficient degradation condition. The optimal experimental conditions for photocatalytic degradation of ammonia-N can be stated as follows: when the concentration of ammonia-N was 20 mg/L, nano-TiO2catalyst dosage was 1.2 g/L, the pH value of solution was 5, H2O2volume fraction was 4 %, respectively, if the reaction time may last 3 h, the removal rate of ammonia-N was expected to reach 92.10 %.


2014 ◽  
Vol 675-677 ◽  
pp. 968-971
Author(s):  
Bin Wang ◽  
Li Ping Qiu ◽  
Li Xin Zhang ◽  
Kang Xie

The performance of biological aerated filter (BAF) with lava media for the treatment of micro-polluted source water was investigated with the operational temperature 18°C, the PH value 6.29-8.35, hydraulic retention time (HRT) 30 min as well as dissolved oxygen (DO) 2-3mg/L. The results show that the lava media is a pretty carrier that has promising surface characteristics for microorganism growth and biofilm formation. The biofilter could be operated successfully in 18d, that was shorter than the ceramic and zeolite media filter in the same start-up condition. During the steady operation period, the lava media BAF performed a promising treatment performance of permanganate index, ammonia nitrogen, and turbidity removal, where as the removal rate were 46%, 97% and 62%, respectively. Moreover, the index of UV254, which partly indicates the concentration of hardly degradable substance in the micro-polluted source water, could be removed 23% in the novel media filter.


Membranes ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 282
Author(s):  
Huining Zhang ◽  
Xin Yuan ◽  
Hanqing Wang ◽  
Shuoqi Ma ◽  
Bixiao Ji

The performance of pollutant removals, tetracycline (TC) and norfloxacin (NOR) removals, membrane fouling mitigation and the microbial community of three Anoxic/Oxic membrane bioreactors (AO-MBRs), including a moving bed biofilm MBR (MBRa), a fixed biofilm MBR (MBRb) and an AO-MBR (MBRc) for control, were compared in treating antibiotic-containing synthetic mariculture wastewater. The results showed that MBRb had the best effect on antibiotic removal and membrane fouling mitigation compared to the other two bioreactors. The maximum removal rate of TC reached 91.65% and the maximum removal rate of NOR reached 45.46% in MBRb. The addition of antibiotics had little effect on the removal of chemical oxygen demand (COD) and ammonia nitrogen (NH4+-N)—both maintained more than 90% removal rate during the entire operation. High-throughput sequencing demonstrated that TC and NOR resulted in a significant decrease in the microbial diversity and the microbial richness MBRs. Flavobacteriia, Firmicutes and Azoarcus, regarded as drug-resistant bacteria, might play a crucial part in the removal of antibiotics. In addition, the dynamics of microbial community had a great change, which included the accumulation of resistant microorganisms and the gradual reduction or disappearance of other microorganisms under antibiotic pressure. The research provides an insight into the antibiotic-containing mariculture wastewater treatment and has certain reference value.


2012 ◽  
Vol 610-613 ◽  
pp. 564-568 ◽  
Author(s):  
Kui Sheng Song ◽  
Xiao Cai Yu ◽  
Dong Dong Hu ◽  
Xu Zheng ◽  
Ji Yao Guo

Nano-ZnO photocatalyst was prepared using direct precipitation method .The crystal form, particle size and configuration characterization of the nano-ZnO prepared was characterized by XRD and SEM. The photocatalytic degradation of ammonia-N in aquaculture wastewater was investigated by using nano-ZnO under UV irradiation. The experimental results show that: nano-ZnO dosage, catalytic reaction time, initial ammonia-N concentration, H2O2volume concentration, pH value affect the efficiency of photocatalytic degradation significantly. On the basis of the results of these experimental data, the optimal experimental conditions for photocatalytic degradation of ammonia-N are tried through the orthogonal test. The optimization experimental conditions for photocatalytic degradation of ammonia-N in aquaculture wastewater are as follows, nano-ZnO catalyst dosage was 0.4 g/L, the pH value of solution was 10, H2O2volume fraction was 4 %, respectively, if the reaction time may last 1 h, the removal rate of ammonia-N was expected to reach 86.66 %.


2011 ◽  
Vol 233-235 ◽  
pp. 733-736
Author(s):  
Wei Hua Song ◽  
Jun Yin ◽  
Jian Hui Wang

A lab-scale study was conducted to precipitate the ammonia from high NH3-N concentration wastewater in the form of magnesium ammonium phosphate(MAP) by applying such chemicals such as MgCl2·6H2O and KH2PO4.The influences of pH value, reactive time and removal rate of ammonia nitrogen were tested.The results shows that the feasible pH values of crystallization and precipitation were between 8 and 10. The structure of struvite crystallization was destroyed under high pH value condition that resulted in ammonia nitrogen dissociating from MAP, which reduced the removal rate of ammonia nitrogen. Results also demonstrated that ammonia nitrogen was effectively reduced from initia1981mg/L to final 5 mg/L, which removal efficiency reached 99% with crystallization and precipitation method when the optimal pH, precipitation time mole ratio of Mg2+, NH4+, PO43- were 8.0, 20 min and 1.4∶1∶1.4 respectively.


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