scholarly journals IgE-binding residues analysis of the house dust mite allergen Der p 23

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sze Lei Pang ◽  
Sri Anusha Matta ◽  
Yang Yie Sio ◽  
Yu Ting Ng ◽  
Yee-How Say ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
House Dust ◽  
Major Allergen ◽  
House Dust Mites ◽  
Molecular Diagnostic ◽  
Diagnostic Strategy ◽  
Dot Blot ◽  
Ige Binding ◽  
Atopic Individual ◽  
Binding Residues

AbstractHouse dust mites (HDMs) are one of the major causes of allergies in the world. The group 23 allergen, Der p 23, from Dermatophagoides pteronyssinus, is a major allergen amongst HDM-sensitized individuals. This study aims to determine the specific immunoglobulin E (sIgE) binding frequency and IgE-binding residues of recombinant Der p 23 (rDer p 23) allergen amongst a cohort of consecutive atopic individuals in a tropical region. We performed site-directed mutagenesis and carried out immuno-dot blot assays using 65 atopic sera. The immuno-dot blot assays results indicated that the two residues K44 and E46 which are located at the N-terminal region are the major IgE-binding residues. The rDerp-23 sIgE titers are strongly correlated to the number of IgE-binding residues for rDer p 23 (P < 0.001). Atopic individuals who were only sensitized to HDM have a significantly higher number of IgE-binding residues than the individuals who were polysensitized to HDM and other crude allergens (P < 0.05). Individuals with allergic multimorbidity and moderate-to-severe allergic rhinitis also have a higher number of IgE-binding residues compared to those with single allergic disease and mild allergic rhinitis. The results prompt us to hypothesize that the individuals who have a higher number of IgE-binding residues may face a bigger challenge to be treated through immunotherapy due to the complexity in designing an effective hypoallergen with a high number of IgE-binding residues. We propose that the development of a refined molecular diagnostic assay, which includes alanine substitution of surface-exposed residues could be a more precise diagnostic strategy to identify all the IgE-binding residues of a major allergen for an atopic individual and the development could be another new dimension in allergy diagnosis and allergen immunotherapy treatment.

scholarly journals The Presence of House Dust Mites in Residences and Classrooms of Students with Allergic Rhinitis

2021 ◽  
Vol 21 (2) ◽  
pp. 138-143
Author(s):  
Suri Dwi Lesmana ◽  
Harianto Harianto ◽  
Reyza Octarient
Keyword(s):  
Allergic Rhinitis ◽  
House Dust ◽  
Health Problem ◽  
Direct Method ◽  
House Dust Mites ◽  
Dust Mites ◽  
Essential Factor ◽  
Skills Lab ◽  
Chronic Stressor ◽  
Large Classrooms

Allergy is still a health problem in Indonesia. One of the manifestations of allergies is allergic rhinitis. Many factors can trigger the recurrence of rhinitis, including exposure to house dust mites. This study aims to identify the population of house dust mites in the classrooms and student residences. This study was participated by 74 FK UNRI students with allergic rhinitis. The samples included dust collected from residences and classrooms consisting of four large classrooms, three small classrooms, four laboratories, two examination rooms, 12 skills lab rooms, and 15 tutorial rooms. Detection of dust was performed using the direct method. The results showed that no classroom (0%) was found with house dust mites. However, there were 37.8% of residences were positive. Based on the result, it can be concluded that the high population of house dust mites in the students’ residences becomes an essential factor as a chronic stressor for allergic rhinitis.

scholarly journals Could FeNO Predict Asthma in Patients with House Dust Mites Allergic Rhinitis?

Medicina ◽  
2020 ◽  
Vol 56 (5) ◽  
pp. 235
Author(s):  
Ioana Adriana Muntean ◽  
Ioana Corina Bocsan ◽  
Stefan Vesa ◽  
Nicolae Miron ◽  
Irena Nedelea ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
House Dust ◽  
Immunoglobulin E ◽  
Biological Evaluation ◽  
House Dust Mites ◽  
Dust Mites ◽  
Persistent Allergic Rhinitis ◽  
Tnf Α ◽  
One Year ◽  
High Level

Background and Objectives: The evolution of allergic rhinitis to asthma is a part of “atopic march”. The aim of this study was to analyze possible predictive markers for asthma occurrence in patients with allergic rhinitis to house dust mites (HDM). Materials and Methods: Fifty-eight patients with persistent allergic rhinitis (PAR) were included. The clinical, biological evaluation and fractionated exhaled nitric oxide (FeNO) measurement were performed at enrolment. The patients were clinically evaluated after one year to determine asthma occurrence. Results: The severity of rhinitis symptoms, levels of total immunoglobulin E (IgE), ICAM-1, VCAM-1, E-selectin and IL-6, but not IL-8 and TNF-α were higher in patients with allergic rhinitis who developed asthma compared to non-asthmatics, but the differences were not significant to considered them as predictive factors for asthma occurrence. The risk of asthma was independently influenced by patients aged over 30 years ((OR-3.74; CI95% 0.86–16.31; p = 0.07), a duration of allergic rhinitis over 12 months ((OR-4.20; CI95% 0.88–20; p = 0.07) and a basal FeNO over 28 parts per billion (pbb) ((OR-18.68; CI95% 3.79–92.05; p < 0.001). Conclusion: Clinical and biological parameters may predict asthma occurrence in patients with persistent allergic rhinitis to HDM. Adult patients with a longer duration of rhinitis symptoms and a high level of FeNO have a greater risk to develop asthma.

Clinical and Immunologic Effects of Sublingual Immunotherapy on Patients with Allergic Rhinitis to House-Dust Mites: 1-Year follow-up Results

2010 ◽  
Vol 24 (4) ◽  
pp. 271-275 ◽  
Author(s):  
Seung-Tae Kim ◽  
Doo Hee Han ◽  
Il Joon Moon ◽  
Chul Hee Lee ◽  
Yang-Gi Min ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
House Dust ◽  
Sublingual Immunotherapy ◽  
House Dust Mites ◽  
Dust Mites

scholarly journals Gambaran Kepadatan Tungau Debu Rumah Sebagai Pencetus Rinitis Alergi pada Anak Panti Asuhan Pekanbaru

2019 ◽  
Vol 13 (1) ◽  
pp. 23
Author(s):  
Yolazenia Yolazenia ◽  
Harianto Harianto ◽  
Iqbal Teguh Riady
Keyword(s):  
Quality Of Life ◽  
Allergic Rhinitis ◽  
House Dust ◽  
International Study ◽  
Descriptive Study ◽  
House Dust Mites ◽  
Dust Mites ◽  
High Incidence ◽  
The Mean

Allergic rhinitis should be considered as a serious condition because it can affect the quality of life of patients. In manycases, the most common allergen is house dust mites (HDM). The purpose of this study was to describe the density ofhouse dust mites as triggers of allergic rhinitis in Pekanbaru orphanage children. This is a descriptive study with across sectional approach. Data obtained from interviews using the modified International Study of Asthma and Allergiesof Childhood (ISAAC) questionnaire to know the allergic rhinitis status and examination of house dust samples toidentify HDM in the parasitology laboratory. Sampling was carried out in 4 orphanages Pekanbaru. Allergic rhinitissymptoms were experienced by 81.2% of children in the orphanages and HDM was found with the mean density 9.11/g of dust. HDM was found in all four orphanages and was found to have a high incidence of allergic rhinitis.

scholarly journals Investigation on risk factors of adult allergic rhinitis in China

2021 ◽  
Author(s):  
Guo Hong ◽  
Zhou Yibo ◽  
Li Fengxia ◽  
Liu Ming Ping ◽  
Cao Yong
Keyword(s):  
Risk Factors ◽  
Allergic Rhinitis ◽  
House Dust ◽  
House Dust Mite ◽  
Air Pressure ◽  
House Dust Mites ◽  
Control Group ◽  
Observation Group ◽  
Dust Mites ◽  
Dust Mite

Abstract OBJECTIVE: To investigate the risk factors associated with allergic rhinitis in adults. METHODS: Using the 1:1 matching principle, 1096 cases of allergic rhinitis patients aged 18-65 years were selected as the observation group, and another group of healthy medical checkups were selected as the control group, and the data related to allergic rhinitis were collected by allergen testing and questionnaire survey, and univariate and multifactorial analyses were performed respectively. RESULTS: The univariate analysis showed that allergic rhinitis may be related to temperature changes, dryness and humidity, air pressure, wind speed, seasonal changes and exposure to allergens such as dust mites and house dust mites. After allergen testing of all patients in the observation group, it was found that dust mite and house dust mite were the highest percentage of allergens, accounting for 40.51%, indicating that environmental factors play a decisive role in allergic rhinitis. Multi-factor analysis showed that exposure to dust mites, house dust mites, Penicillium punctatum, Streptomyces crossatus and Aspergillus fumigatus were independent risk factors for allergic rhinitis when it was humid, when it was cold, when it was windy and when the air pressure was low. CONCLUSION: The epidemiological status of allergic rhinitis in adults in China was initially understood, and the main allergens were house dust mite and dust mite, which provided scientific epidemiological information for the standardized prevention and treatment of allergic rhinitis in this region.

scholarly journals High Dust Mite specific Immunoglobulin E (sIgE): a Promising Indicator of Allergic Rhinitis to Asthma

2020 ◽  
Author(s):  
Rui Tang ◽  
Xiaohong Lyu ◽  
Yuelun Zhang ◽  
Shi Chen ◽  
Hong Li
Keyword(s):  
Allergic Rhinitis ◽  
House Dust ◽  
House Dust Mite ◽  
Immunoglobulin E ◽  
House Dust Mites ◽  
Kaplan Meier ◽  
Sige Level ◽  
Specific Immunoglobulin E ◽  
Dust Mite ◽  
Specific Immunoglobulin

Abstract Background: House dust mites are the most prevalent allergens in patients with asthma and/or rhinitis in China. Cross-sectional data in 2009 have shown that allergic rhinitis often preceded or occurred at the same time as asthma in patients which was used to investigate the association of serum specific immunoglobulin E (sIgE) levels to house dust mite with the onset of asthma in patients with allergic rhinitis. Methods: 321 patients with allergic rhinitis were face-to-face interviewed and underwent sIgE tests to house dust mite. The temporal sequence of allergic rhinitis and asthma was documented. Univariate analysis, multinomial logistic regression, and Kaplan-Meier survival analysis were performed. Results: Of the 321 participants, 213 (66.4%) had asthma, which occurred after or simultaneously with rhinitis, and 108 (33.6%) suffered from allergic rhinitis only. After controlling basic parameters, factors correlated to sIgE, and essential factors considered by clinical allergists, the risk of developing asthma always increased with the levels of sIgE to house dust mite in all four models (p < 0.01). In Kaplan–Meier analysis, in the first ten years with allergy rhinitis, a high sIgE level represented a high probability of the coexistence of allergic rhinitis and asthma (p < 0.01). For house dust mite sIgE level 5-6, 5 years Rhinitis-Asthma Conversion Rate (RACR) had reached almost 70%. Conclusion: High-level house dust mite sIgE can exist as an indicator of rhinitis to asthma. It provides a theoretical basis for early intervention in patients with high sIgE levels in order to prevent asthma. This assessment and intervention should be performed at the early stage of rhinitis.

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