scholarly journals Cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xia Chen ◽  
Zhaoxiang He ◽  
Xingzheng Li ◽  
Jianlou Song ◽  
Mingyi Huang ◽  
...  
Keyword(s):  
Laying Hens ◽  
Egg Quality ◽  
Genetic Regulation ◽  
Reproductive Hormones ◽  
Poultry Production ◽  
White Leghorn ◽  
Factors Affecting ◽  
Qpcr Analysis ◽  
Deposition Duration ◽  
Major Factors

AbstractThe cuticle formed in the uterus is the outermost layer as the first defense line of eggshell against microbial invasions in most avian species, and analyzing its genetic regulation and influencing factors are of great importance to egg biosecurity in poultry production worldwide. The current study compared the uterine transcriptome and proteome of laying hens producing eggs with good and poor cuticle quality (GC and PC, the top and tail of the cuticle quality distribution), and identified several genes involved with eggshell cuticle quality (ESCQ). Overall, transcriptomic analysis identified 53 differentially expressed genes (DEGs) between PC versus GC group hens, among which 25 were up-regulated and 28 were down-regulated. No differences were found in the uterine proteome. Several DEGs, including PTGDS, PLCG2, ADM and PRLR related to uterine functions and reproductive hormones, were validated by qPCR analysis. Egg quality measurements between GC and PC hens showed GC hens had longer laying interval between two consecutive ovipositions (25.64 ± 1.23 vs 24.94 ± 1.12 h) and thicker eggshell thickness (352.01 ± 23.04 vs 316.20 ± 30.58 μm) (P < 0.05). Apart from eggshell traits, other egg quality traits didn’t differ. The result demonstrated eggshell and cuticle deposition duration in the uterus is one of the major factors affecting ESCQ in laying hens. PTGDS, PLCG2, ADM and PRLR genes were discovered and might play crucial roles in cuticle deposition by regulating the uterine muscular activities and secretion function. The findings in the present study provide new insights into the genetic regulation of cuticle deposition in laying hens and establish a foundation for further investigations.

Cuticle Deposition Duration In The Uterus Is One Of The Major Factors Affecting Eggshell Cuticle Quality In White Leghorn Laying Hens

2021 ◽  
Author(s):  
Xia Chen ◽  
Zhaoxiang He ◽  
Xingzheng Li ◽  
Jianlou Song ◽  
Mingyi Huang ◽  
...  
Keyword(s):  
Laying Hens ◽  
Egg Quality ◽  
Activity Rhythm ◽  
Expression Patterns ◽  
Genetic Regulation ◽  
Reproductive Hormones ◽  
Poultry Production ◽  
Factors Affecting ◽  
Deposition Duration ◽  
Major Factors

Abstract Background: The cuticle formed in the uterus is the outermost layer as the first defense line of eggshell against microbial invasions in most avian species, and analyzing its genetic regulation and influencing factors are of great importance to egg biosecurity in poultry production worldwide. The current study compared the uterine transcriptome and proteome of laying hens producing eggs with good and poor cuticle quality (GC and PC, the top and tail of the cuticle quality distribution), and identified several genes involved with eggshell cuticle quality (ESCQ).Results: Overall, transcriptomic analysis identified 53 differentially expressed genes (DEGs) between PC vs GC group hens, among which 25 were up-regulated and 28 were down-regulated. No differences were found in the uterine proteome. Several DEGs, including PTGDS, PLCG2, ADM and PRLR related to uterine functions and reproductive hormones, were validated by qPCR analysis. Egg quality measurements between GC and PC hens showed GC hens had longer laying interval between two consecutive ovipositions (25.64 ± 1.23 vs 24.94 ± 1.12 h) and thicker eggshell thickness (352.01 ± 23.04 vs 316.20 ± 30.58 μm) (P < 0.05). Apart from eggshell traits, other egg quality traits didn’t differ. The results above suggested the expression patterns of PTGDS, PLCG2, ADM and PRLR genes in the uterus regulated the muscular activity rhythm and secretion function, which ensured the eggshell and cuticle deposition duration, and lead to the good ESCQ of the GC hens.Conclusions：The present study demonstrated eggshell and cuticle deposition duration in the uterus is one of the major factors affecting ESCQ in laying hens. PTGDS, PLCG2, ADM and PRLR genes were discovered and might play crucial roles in cuticle deposition by regulating the uterine muscular activities and secretion function. The findings in the present study provide new insights into the genetic regulation of cuticle deposition in laying hens and establish a foundation for further investigations.

scholarly journals Cuticle Secretion Duration in the Uterus Is One of the Major Factors Affecting Eggshell Cuticle Deposition in White Leghorn Laying Hens

2020 ◽  
Author(s):  
Xia CHEN ◽  
Zhaoxiang HE ◽  
Xingzheng LI ◽  
Jianlou SONG ◽  
Mingyi HUANG ◽  
...  
Keyword(s):  
Laying Hens ◽  
Egg Quality ◽  
Activity Rhythm ◽  
Expression Patterns ◽  
Genetic Regulation ◽  
Reproductive Hormones ◽  
Poultry Production ◽  
Factors Affecting ◽  
Laying Performance ◽  
Major Factors

Abstract Background: The cuticle formed in the uterus is the outermost layer as the first defense line of eggshell against microbial invasions in most avian species, and analyzing its genetic regulation and influencing factors are of great importance to egg biosecurity in poultry production. The current study compared the uterine transcriptome and proteome of laying hens producing eggs with good and poor cuticle deposition (GC and PC, the top and tail of the cuticle deposition distribution), and identified several genes involved with eggshell cuticle deposition.Results: Overall, transcriptomic analysis identified 53 differentially expressed genes (DEGs) between PC vs GC group hens, among which 25 were up-regulated and 28 were down-regulated. No differences were found in the uterine proteome. Several up-regulated DEGs of PC vs GC hen uterus, including PTGDS, PLCG2, ADM and PRLR related to uterine functions and reproductive hormones, were validated by qPCR analysis. Phenotypic measurements for verification between GC and PC hens showed GC hens had longer laying interval between two consecutive ovipositions (25.64 ± 1.23 vs 24.94 ± 1.12 h), thicker eggshell thickness (352.01 ± 23.04 vs 316.20 ± 30.58 μm) and more laying days (20.67 ± 2.58 vs 18.48 ± 2.46) within 27 laying days (P < 0.05). Apart from eggshell traits, other egg quality traits didn’t differ. The results above suggested that the expression patterns of PTGDS, PLCG2, ADM and PRLR genes in the uterus regulated the muscular activity rhythm and secretion function, which ensured the eggshell and cuticle formation duration, and lead to the good eggshell and cuticle quality of the GC hens.Conclusions: The present study demonstrated eggshell and cuticle secretion duration in the uterus is one of the major factors affecting eggshell cuticle deposition in laying hens. PTGDS, PLCG2, ADM and PRLR genes were discovered and might play crucial roles in cuticle deposition by regulating the uterine muscular activities and secretion function. Moreover, the cuticle is associated with the eggshell quality and laying performance, implying the importance of selection to the cuticle trait in laying hens. The findings in present study provide new insights into the genetic regulation of cuticle deposition in laying hens and establish a foundation for further investigations.

scholarly journals Analysis of Factors Affecting Year-Round Performance of Single Comb White Leghorn Laying Hens Reared Under an Open-Sided Housing System

1988 ◽  
Vol 67 (8) ◽  
pp. 1130-1138 ◽  
Author(s):  
J. OKUMURA ◽  
N. MORI ◽  
T. MURAMATSU ◽  
I. TASAKI ◽  
F. SAITO
Keyword(s):  
Laying Hens ◽  
Housing System ◽  
White Leghorn ◽  
Factors Affecting ◽  
Single Comb

scholarly journals Laying Hens Biochar Diet Supplementation—Effect on Performance, Excreta N Content, NH3 and VOCs Emissions, Egg Traits and Egg Consumers Acceptance

Agriculture ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 237 ◽  
Author(s):  
Kajetan Kalus ◽  
Damian Konkol ◽  
Mariusz Korczyński ◽  
Jacek A. Koziel ◽  
Sebastian Opaliński
Keyword(s):  
Feed Intake ◽  
Laying Hens ◽  
Egg Quality ◽  
Dry Matter Content ◽  
Poultry Production ◽  
N Content ◽  
Total N ◽  
Voc Emissions ◽  
Sustainable Solutions ◽  
Laying Performance

Sustainable solutions for intensive poultry production can help farmers, rural communities, consumers, and regulatory agencies. This study assessed supplementation of laying hens diet with beechwood biochar (BC, 1~2%) and BC–aluminosilicates–glycerin mixture (BCM, 1.5~3%) to lower the environmental impact while maintaining egg quality. The effect on feed intake, laying performance, egg quality, the sensory quality of hardboiled eggs, ammonia (NH3) and volatile organic compound (VOC) emissions from excreta, and the excreta composition, were evaluated. A total of 90 hens were distributed into 30 cages and divided into five groups (n=6 replications). BC addition increased daily feed intake, while 1.5% BCM addition reduced it. The influence on egg parameters was positive, with a 6% increase in laying performance, up to 10% and 6% increase in shell resistance to crushing and shell thickness, respectively. The sensory analysis demonstrated no significant differences between all treatments. Excreta total N content was numerically lower due to the treatments (by 4~20%); its pH increased (not significantly), while no effect on ammoniacal N and dry matter content was observed. Most of the investigated treatments had a numerically positive (not statistically significant) effect on NH3 reduction. The reduction of VOC emissions was ambiguous and not statistically significant.

scholarly journals Use of Medicinal Mushrooms in Layer Ration

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1014
Author(s):  
Shad Mahfuz ◽  
Xiangshu Piao
Keyword(s):  
Health Status ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Production Performance ◽  
Poultry Feed ◽  
Poultry Production ◽  
Medicinal Mushrooms ◽  
Laying Performance ◽  
Medicinal Fungi

Application of different medicinal mushrooms intended to enhance production performance and health status has created an importance demand in poultry production. One goal of using medicinal mushrooms is to get rid of antibiotics in poultry feed without affecting the optimum performance. Increasing concerns about this issue have led to more attention on antibiotic substitutes and a significant demand for them for organic egg production. Thus, supplementation with medicinal mushrooms is a new concept for research in layer production, however, there is still a great deal of confusion about inclusion levels and the mode of action of medicinal mushrooms on production performance and health status in laying hens. Taking this into account, this review outlines the experimental uses of medicinal fungi on the growth performance, laying performance, egg quality, and health status of layer birds based on previous findings to date. Finally, we highlight that supplementation with medicinal fungi can play a role on the immunity, health, and production performance in laying hens.

scholarly journals Fermented Astragalus in diet improved laying performance, egg quality, antioxidant and immunological status and intestinal microbiota in laying hens

2020 ◽  
Author(s):  
Hongtao Shi ◽  
Baiyu Wang ◽  
Chuanzhou Bian ◽  
Yingqian Han ◽  
Hongxing Qiao
Keyword(s):  
Intestinal Microbiota ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Feed Additive ◽  
Control Group ◽  
Poultry Production ◽  
Immunological Status ◽  
Laying Performance ◽  
Significant Difference

Abstract In the era of increased antibiotic resistance and ever stricter control on antibiotic use, it is urgent to develop green, safe and non-residue alternatives to antibiotics applied to the poultry industry. To this end, we supplied the potential Lactobacillus Plantarum (L. Plantarum) fermented Astragalus in the diet of laying hens, with a final addition of 3‰. Its effects have been assessed on laying performance, egg quality, antioxidant and immunological status and intestinal microbiota, and are compared to the control group, to the Astragalus group containing 3‰ unfermented Astragalus, and to the L. Plantarum group containing 2% L. Plantarum (1 × 108 CFU/mL). During the second half of the experimental period (15 to 28 days), the egg production rate was significantly higher in the fermented Astragalus group than that in the other groups, with the fermented Astragalus group having the lowest feed conversion ratio. No significant difference (P > 0.05) was observed among treatments on egg quality. Fermented Astragalus-treated hens exhibited significantly increased catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in serum, and reduced malondialdehyde (MDA) in serum. Furthermore, fermented Astragalus supplementation resulted in a significant increase in ileal microbiota abundance relative to control. In conclusion, feeding laying hens with L. Plantarum fermented Astragalus has beneficial effects on production, antioxidant potential, immunity and ileal microbiota. L. Plantarum fermented Astragalus is expected to be a novel feed additive used in poultry production.

scholarly journals Dietary Addition of Astragalus Fermented by Lactobacillus Plantarum Improved Laying Performance, Egg Quality, Antioxidant and Immunological Status and Intestinal Microbiota in Laying Hens

2020 ◽  
Author(s):  
Hongtao Shi ◽  
Baiyu Wang ◽  
Chuanzhou Bian ◽  
Yingqian Han ◽  
Hongxing Qiao
Keyword(s):  
Lactobacillus Plantarum ◽  
Intestinal Microbiota ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Control Group ◽  
Poultry Production ◽  
Immunological Status ◽  
Laying Performance ◽  
Significant Difference

Abstract Background In the era of increased antibiotic resistance and ever stricter control on antibiotic use, it is urgent to develop green, safe and non-residue alternatives to antibiotics applied to the poultry industry. To this end, we supplied the potential Lactobacillus Plantarum (L. Plantarum) fermented Astragalus in the diet of laying hens, with a final addition of 3‰. Its effects have been assessed on laying performance, egg quality, antioxidant and immunological status and intestinal microbiota, and are compared to the control group, to the Astragalus group containing 3‰ unfermented Astragalus, and to the L. Plantarum group containing 2% L. Plantarum (1 × 108 CFU/mL). Results During the second half of the experimental period (15 to 28 days), the egg production rate was significantly higher in the fermented Astragalus group than that in the other groups, with the fermented Astragalus group having the lowest feed conversion ratio. No significant difference (P > 0.05) was observed among treatments on egg quality. Fermented Astragalus-treated hens exhibited significantly increased catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in serum, and reduced malondialdehyde (MDA) in serum. Furthermore, fermented Astragalus supplementation resulted in a significant increase in ileal microbiota abundance relative to control. Conclusions Feeding laying hens with L. Plantarum fermented Astragalus has beneficial effects on production, antioxidant potential, immunity and ileal microbiota. L. Plantarum fermented Astragalus is expected to be a novel feed additive used in poultry production.

EVALUATION OF FULL-FAT SPAN RAPESEED AS AN ENERGY AND PROTEIN SOURCE FOR LAYING HENS

1975 ◽  
Vol 55 (2) ◽  
pp. 219-222 ◽  
Author(s):  
J. M. OLOMU ◽  
A. R. ROBBLEE ◽  
D. R. CLANDININ ◽  
R. T. HARDIN
Keyword(s):  
Egg Production ◽  
Dry Matter ◽  
Laying Hens ◽  
Egg Quality ◽  
White Leghorn ◽  
Feed Conversion ◽  
Low Erucic Acid ◽  
Dietary Treatments ◽  
Single Comb ◽  
Simple Regression

Full-fat Span rapeseed (a low erucic acid variety), which had been steam-treated at 90 C for 30 min and then ground, was examined as a protein and energy source in rations of Single Comb White Leghorn laying hens during a laying period of 336 days. The levels of mortality and egg production were not significantly affected by the inclusion of 0, 5, 10 or 15% Span rapeseed in the ration; however, calculation of the simple regression between hen-day production and level of rapeseed used indicated a significant decrease in production with increasing levels of rapeseed in the ration. No mortality was attributed to ’haemorrhagic liver syndrome’ in the groups fed rations containing 0 and 5% rapeseed but the incidence of haemorrhagic liver syndrome was 3.1 and 4.2%, respectively, in birds fed rations containing 10 and 15% rapeseed. Egg quality, feed conversion and body weight were not affected by any of the dietary treatments. The sizes of the livers and hearts and their composition of dry matter, fat and protein were not significantly affected by dietary treatments. There was, however, a progressive and significant (P < 0.05) increase in the relative weights of the thyroids as the level of rapeseed in the ration was increased.

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