scholarly journals Dietary Addition of Astragalus Fermented by Lactobacillus Plantarum Improved Laying Performance, Egg Quality, Antioxidant and Immunological Status and Intestinal Microbiota in Laying Hens

2020 ◽  
Author(s):  
Hongtao Shi ◽  
Baiyu Wang ◽  
Chuanzhou Bian ◽  
Yingqian Han ◽  
Hongxing Qiao

Abstract Background In the era of increased antibiotic resistance and ever stricter control on antibiotic use, it is urgent to develop green, safe and non-residue alternatives to antibiotics applied to the poultry industry. To this end, we supplied the potential Lactobacillus Plantarum (L. Plantarum) fermented Astragalus in the diet of laying hens, with a final addition of 3‰. Its effects have been assessed on laying performance, egg quality, antioxidant and immunological status and intestinal microbiota, and are compared to the control group, to the Astragalus group containing 3‰ unfermented Astragalus, and to the L. Plantarum group containing 2% L. Plantarum (1 × 108 CFU/mL). Results During the second half of the experimental period (15 to 28 days), the egg production rate was significantly higher in the fermented Astragalus group than that in the other groups, with the fermented Astragalus group having the lowest feed conversion ratio. No significant difference (P > 0.05) was observed among treatments on egg quality. Fermented Astragalus-treated hens exhibited significantly increased catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in serum, and reduced malondialdehyde (MDA) in serum. Furthermore, fermented Astragalus supplementation resulted in a significant increase in ileal microbiota abundance relative to control. Conclusions Feeding laying hens with L. Plantarum fermented Astragalus has beneficial effects on production, antioxidant potential, immunity and ileal microbiota. L. Plantarum fermented Astragalus is expected to be a novel feed additive used in poultry production.

2020 ◽  
Author(s):  
Hongtao Shi ◽  
Baiyu Wang ◽  
Chuanzhou Bian ◽  
Yingqian Han ◽  
Hongxing Qiao

Abstract In the era of increased antibiotic resistance and ever stricter control on antibiotic use, it is urgent to develop green, safe and non-residue alternatives to antibiotics applied to the poultry industry. To this end, we supplied the potential Lactobacillus Plantarum (L. Plantarum) fermented Astragalus in the diet of laying hens, with a final addition of 3‰. Its effects have been assessed on laying performance, egg quality, antioxidant and immunological status and intestinal microbiota, and are compared to the control group, to the Astragalus group containing 3‰ unfermented Astragalus, and to the L. Plantarum group containing 2% L. Plantarum (1 × 108 CFU/mL). During the second half of the experimental period (15 to 28 days), the egg production rate was significantly higher in the fermented Astragalus group than that in the other groups, with the fermented Astragalus group having the lowest feed conversion ratio. No significant difference (P > 0.05) was observed among treatments on egg quality. Fermented Astragalus-treated hens exhibited significantly increased catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in serum, and reduced malondialdehyde (MDA) in serum. Furthermore, fermented Astragalus supplementation resulted in a significant increase in ileal microbiota abundance relative to control. In conclusion, feeding laying hens with L. Plantarum fermented Astragalus has beneficial effects on production, antioxidant potential, immunity and ileal microbiota. L. Plantarum fermented Astragalus is expected to be a novel feed additive used in poultry production.


2020 ◽  
Author(s):  
Hongtao Shi ◽  
Baiyu Wang ◽  
Chuanzhou Bian ◽  
Yingqian Han ◽  
Hongxing Qiao

Abstract In the era of increased antibiotic resistance and ever-stricter control on antibiotic use, it is urgent to develop green, safe, and non-residue alternatives to antibiotics applied to the poultry industry. To this end, we supplied the potential Lactobacillus Plantarum (L. Plantarum) fermented Astragalus in the diet of laying hens, with a final addition of 3‰. Its effects have been assessed on laying performance, egg quality, antioxidant and immunological status, and intestinal microbiota, and are compared to the control group, to the Astragalus group containing 3‰ unfermented Astragalus, and to the L. Plantarum group containing 2% L. Plantarum (5×108 colony-forming unit (CFU) per milliliter (mL)). During the second half of the experimental period (15 to 28 days), the egg production rate was considerably higher in the fermented Astragalus group than that in the other groups, with the fermented Astragalus group having the lowest feed conversion ratio. No significant difference (P>0.05) was noted among treatments on egg quality. Fermented Astragalus-treated hens exhibited significantly increased catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in serum, and reduced malondialdehyde (MDA) in serum. Furthermore, fermented Astragalus supplementation resulted in a significant increase in ileal microbiota abundance relative to control. In conclusion, feeding laying hens with L. Plantarum fermented Astragalus has beneficial effects on production, antioxidant potential, immunity, and ileal microbiota. L. Plantarum fermented Astragalus is expected to be a novel feed additive used in poultry production.


2021 ◽  
Vol 8 ◽  
Author(s):  
Kai Qiu ◽  
Uchechukwu Edna Obianwuna ◽  
Jing Wang ◽  
Hai-jun Zhang ◽  
Guang-hai Qi ◽  
...  

In the context of increasing awareness on the dietary supplementation of organic selenium in commercial poultry production and ensuring safe egg production, the present study investigated the effects of selenium on the pharmacokinetics of the therapeutic use of florfenicol and enrofloxacin from perspectives of laying performance, selenium deposition in eggs, and drug residue in plasma, organs, and eggs. A 2 × 3 factorial arrangement with two kinds of drugs (florfenicol vs. enrofloxacin, 200 mg/kg) and three levels of dietary organic selenium SCIP (selenium conjugated to insect protein) (0, 2, and 5 mg/kg) was designed together with a blank control group. Healthy Hy-Line Brown laying hens (n = 252, 40-week-old and 90.0 ± 1.7% of egg production rate) were randomly allocated into one of seven treatments with six replicates and six hens per replicate. The experiment lasted for 42 days and consisted of three periods (adjusted stage, depositional stage, and eliminating stage) of 14 days each. These stages entail feeding of the laying hens with basal diets, addition of drugs and selenium synchronously into the diets, drug withdrawal from diet, and supply of selenium uninterruptedly in the diet. Egg production and feed intake were recorded on daily and weekly bases, respectively. The selenium content in egg yolk, egg white, and whole eggs and the drug residues in eggs, plasma, liver, kidney, and breast muscle were determined on days 2, 3, 5, 6, 7, 9, 11, and 14 of the depositional and eliminating stages. There was no significant difference (p > 0.05) in egg production among the dietary treatments, but feed intake decreased significantly (p < 0.05) in the drug treatment group compared to other groups. Dietary organic selenium decreased the residue of drugs in tissues and eggs, while the metabolism and deposition of selenium in laying hens were suppressed due to drug effects. The results of the present study are of significance to enrich the knowledge of the pharmacokinetics of florfenicol and enrofloxacin in laying hens and ensure the quality of poultry products.


2017 ◽  
Vol 2 (4) ◽  
pp. 547-554 ◽  
Author(s):  
Mst Nasrin Banu ◽  
Md Bazlar Rashid ◽  
Md Mahmudul Hasan ◽  
Fahima Binte Aziz ◽  
Md Rakibul Islam ◽  
...  

The study was conducted to determine the effect of antiprolactin drug (Bromergon®) and Peppermint (Mentha piperita L.) on broodiness, laying performance and egg quality in indigenous hens. The effect of modulation of prolactin concentration on egg production, sequence length and inter sequence pauses were studied by analyzing the oviposition records. Total sixty indigenous laying hens (30-40 weeks of age), were assigned for treatment with peppermint solution and Bromergon®. Sixty laying hens were randomly divided into 4 groups (A, B, C and D) and each group remained 15 hens. Group A was kept for control, Group B was treated with bromocriptine (Bromergon® SANDOZ) orally @ 640 ?g per bird per day, Group C with 50% peppermint solution @ 10 g per bird orally and Group D was with peppermint and Bromergon® combined at previous dose. Over the course of trial, incremental dietary peppermint (Group C) significantly (p?0.05) increased egg production, body weight of treated indigenous hens than other groups. Egg shell percentage, thickness and haugh unit of hens fed diets supplemented with peppermint were greater than that of hens fed the control diet. However, peppermint supplementation did not influence other egg quality characteristics like albumen and yolk percentages and albumen height. The treated birds had comparatively longer sequences and fewer pauses. It is concluded that the physiological pauses occur during ovulatory sequences can be disrupted effectively using Bromergon® and peppermint. Prolactin levels modulated which may interfere with follicular recruitment and subsequent oviposition thereby improves egg laying potential of the indigenous hens.Asian J. Med. Biol. Res. December 2016, 2(4): 547-554


Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1014
Author(s):  
Shad Mahfuz ◽  
Xiangshu Piao

Application of different medicinal mushrooms intended to enhance production performance and health status has created an importance demand in poultry production. One goal of using medicinal mushrooms is to get rid of antibiotics in poultry feed without affecting the optimum performance. Increasing concerns about this issue have led to more attention on antibiotic substitutes and a significant demand for them for organic egg production. Thus, supplementation with medicinal mushrooms is a new concept for research in layer production, however, there is still a great deal of confusion about inclusion levels and the mode of action of medicinal mushrooms on production performance and health status in laying hens. Taking this into account, this review outlines the experimental uses of medicinal fungi on the growth performance, laying performance, egg quality, and health status of layer birds based on previous findings to date. Finally, we highlight that supplementation with medicinal fungi can play a role on the immunity, health, and production performance in laying hens.


Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 15 ◽  
Author(s):  
Yongxiang Wei ◽  
Weichao Zheng ◽  
Baoming Li ◽  
Qin Tong ◽  
Haipeng Shi ◽  
...  

Caged laying hens are prone to calcium deficiencies, resulting in osteoporosis and egg quality deterioration during the later phase of the laying cycle. Fluorescent light and light-emitting diodes (LEDs), which are widely used in poultry houses now, are both deficient in ultraviolet (UV) light, the lack of which is detrimental to chickens’ welfare and health. This study was conducted to investigate the effects of UVB light supplementation using LEDs on the bone traits, blood parameters, laying performance, and egg quality for caged laying hens at 68–75 weeks. In total, 120 Jingfen laying hens were randomly assigned to four different groups, with three replicates in each group (10 hens in each cage as a replicate). UVB-LED lamps installed under the feed troughs were used to provide UVB light (296–316 nm) for the birds in the three treatment groups (1 h, 2 h, and 3 h UVB supplementation per day, respectively), while the control group was not exposed to UVB-LED light. Bone traits, egg quality, and amounts of calcium (Ca), phosphorus (P), 25-hydroxyvitamin D3 (25(OH)D3), 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), and 7-dehydrocholesterol (7-DHC) in both the serum and egg yolks were tested during the experiment. The results demonstrated that UVB-LED exposure significantly increased the bone mineral density (BMD), egg production, and yolk 1,25(OH)2D3 concentrations (p < 0.05), and reduced the content of serum 7-DHC (p < 0.05), especially in the 2 h/day group; however, it did not improve egg quality, vitamin D metabolites, or photoproducts in the serum and yolk 25(OH)2D3 concentrations (p > 0.05). This study concluded that UVB supplementation using LEDs had a positive effect on caged laying hens during the later phase of the laying cycle.


Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1381
Author(s):  
Tzuen-Rong J Tzeng ◽  
Tzu-Yu Liu ◽  
Chiao-Wei Lin ◽  
Pei-En Chang ◽  
Pei-Xin Liao ◽  
...  

Alternative growth promoters are able to not only effectively replace the traditional use of antibiotics but also provide additional health benefits for livestock and reduce food safety concerns. This study investigated the effects of dry Hydrastis canadensis on the laying performance and fecal microbial community of laying hens. Twenty-four Lohmann (LSL, white layer strain) hens were reared from 40 to 48 weeks of age and randomly allotted to four dietary treatments (six birds/treatment). The dietary treatments comprised a basal diet with no treatment as control, a basal diet plus 0.6% powder of dry Hydrastis canadensis roots (R) or leaves (L), and a basal diet plus 0.6% powder of a mixture of dry Hydrastis canadensis roots and leaves (1:1, LR). No mortality was observed in the whole experimental period. The results indicated that albumen height in the LR group was significantly greater than that in the control group. The diet supplemented with Hydrastis canadensis had no significant effects on egg production rate, egg weight, eggshell strength, eggshell thickness, Haugh unit, or yolk height during the whole experimental phase. However, principal coordinate analysis, comparative heat map analysis, and cluster dendrogram analysis of cecal microbiota showed distinct clusters among the groups treated with Hydrastis canadensis and the control group. Regarding blood biochemical parameters, serum cholesterol levels were significantly lower in all Hydrastis canadensis-treated groups compared with those in the control group. Moreover, serum low-density lipoprotein levels were lower in hens supplemented with the leaf of Hydrastis canadensis. The abundances of the phyla Fusobacteria and Kiritimatiellaeota were increased (p < 0.05) in laying hens fed with 0.6% Hydrastis canadensis leaves, whereas the abundance of the phylum Firmicutes in cecum digesta decreased in response to treatment with Hydrastis canadensis roots and leaves. The relative abundance of the Fusobacterium genus was higher in the LR group compared with that in the control. On the contrary, we found a different trend in the Synergistes genus. The potential influences of these microbiota on the performance of laying hens were discussed. The results demonstrate that Hydrastis canadensis can improve the egg albumen height and modulate the cecum digesta microbiota composition of laying hens.


2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Miao Ye ◽  
Chunjie Wei ◽  
Anam Khalid ◽  
Qian Hu ◽  
Ru Yang ◽  
...  

Abstract Background The excessive use of antibiotics in the livestock feed industry caused inevitable side effects of microbial resistance. Besides this residual antibiotics in animal-derived foodstuff imposed serious health problems for humans. So this study aimed to investigate the potential use of Bacillus velezensis to substitute antibiotics for poultry production. A total of 468, 49-week-old Hy-Line Brown chickens, were randomly divided into four groups the control group (regular diet), experiment group I (0.1% B. veleznesis), experiment group II (0.2% B. veleznesis), and antibiotic group (50 mg/kg flavomycin), with three replicates per group and trial period consisted on 42 days. Results The results showed that, compared with the control group, the average egg production rate and daily feed intake of experimental groups I and II increased significantly (P < 0.05), while the average egg weight was increased in experimental group II as compared to (I) (P < 0.01). The feed conversion ratio was decreased (P > 0.05) in group (II) Egg quality parameters such as yolk weight of the experimental group II was increased, but that of the antibiotic group and experiment group I was decreased, neither significant (P > 0.05). Moreover, the eggshell strength, yolk color, albumen height, and Haugh unit were significantly increased (P < 0.05). Compared with the control group, probiotic groups can increase the progesterone and motilin (P > 0.05) but decrease the secretin and cholecystokinin in the blood plasma (P > 0.05). Conclusions This study suggested that B. velezensis can substitute in-feed-antibiotics and improved most of the study parameters significantly. Which suggested that B. velezensis has potential future application value to replace the feed antibiotics.


2019 ◽  
Vol 59 (8) ◽  
pp. 1553 ◽  
Author(s):  
S. S. Diarra ◽  
I. Wamekeni ◽  
A. Vunagilee ◽  
H. Lavaka ◽  
T. Finau

The effects of peeling and limestone flour level on the utilisation of Alocasia macrorrhiza corm meal (ACM) by laying hens were investigated. Maize-based diets with two concentrations of limestone flour (40 and 60 g/kg) and diets containing 200 g peeled and unpeeled ACM meal/kg with the same levels of limestone flour were fed to five replicate pens containing 10 20-week-old Shaver brown pullets (1635 ± 148 g), each for 105 days. Results showed no interaction or main effects of ACM or limestone concentration on feed intake, egg weight, egg mass, feed conversion efficiency, egg shape index, surface area and Haugh unit (P &gt; 0.05). Feeding whole ACM with 40 g limestone/kg diet reduced hen-day production and shell thickness (P &lt; 0.05) but this was overcome by increasing limestone concentration to 60 g/kg diet. Peeled ACM with both concentrations of limestone flour maintained laying performance and egg quality. We concluded that feeding 200 g whole ACM depresses egg production and shell quality in laying hens, but increasing dietary limestone concentration overcomes this adverse effect. The concentration of limestone flour in the diet has no effect on laying performance. Higher inclusion levels of ACM, calcium source and level that will maintain laying performance and reduce feed cost need to be investigated.


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