scholarly journals Stage specific comparative transcriptomic analysis to reveal gene networks regulating iron and zinc content in pearl millet [Pennisetum glaucum (L.) R. Br.]

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
C. Tara Satyavathi ◽  
Rukam S. Tomar ◽  
Supriya Ambawat ◽  
Jasminkumar Kheni ◽  
Shital M. Padhiyar ◽  
...  
Keyword(s):  
Pearl Millet ◽  
Gene Networks ◽  
Pennisetum Glaucum ◽  
Gene Annotation ◽  
Zinc Content ◽  
Climatic Conditions ◽  
High Yield ◽  
Poor People ◽  
Zinc Metabolism ◽  
Iron And Zinc

AbstractPearl millet is an important staple food crop of poor people and excels all other cereals due to its unique features of resilience to adverse climatic conditions. It is rich in micronutrients like iron and zinc and amenable for focused breeding for these micronutrients along with high yield. Hence, this is a key to alleviate malnutrition and ensure nutritional security. This study was conducted to identify and validate candidate genes governing grain iron and zinc content enabling the desired modifications in the genotypes. Transcriptome sequencing using ION S5 Next Generation Sequencer generated 43.5 million sequence reads resulting in 83,721 transcripts with N50 of 597 bp and 84.35% of transcripts matched with the pearl millet genome assembly. The genotypes having high iron and zinc showed differential gene expression during different stages. Of which, 155 were up-regulated and 251 were down-regulated while during flowering stage and milking stage 349 and 378 transcripts were differentially expressed, respectively. Gene annotation and GO term showed the presence of transcripts involved in metabolic activities associated with uptake and transport of iron and zinc. Information generated will help in gaining insights into iron and zinc metabolism and develop genotypes with high yield, grain iron and zinc content.

scholarly journals Stage Specific Comparative Transcriptomic Analysis To Reveal Gene Networks Regulating Iron And Zinc Content In Pearl Millet [Pennisetum glaucum (L.) R. Br.]

2021 ◽  
Author(s):  
C. Tara Satyavathi ◽  
Rukam S. Tomar ◽  
Supriya Ambawat ◽  
Jasminkumar Kheni ◽  
Shital M. Padhiyar ◽  
...  
Keyword(s):  
Pearl Millet ◽  
Gene Networks ◽  
Pennisetum Glaucum ◽  
Gene Annotation ◽  
Zinc Content ◽  
High Yield ◽  
Poor People ◽  
Zinc Metabolism ◽  
Iron And Zinc ◽  
Metabolic Activities

Abstract Pearl millet is an important staple food crop of poor people which is rich in micronutrients like iron and zinc and amenable for focused breeding for these micronutrients along with high yield. Transcriptome sequencing using ION S5 Next Generation Sequencer generated 43.5 million sequence reads resulting in 83,721 transcripts with N50 of 597 bp and 84.35% of transcripts matched with the pearl millet genome assembly. The genotypes having high Fe and Zn showed differential gene expression during different stages. Of which, 155 were up-regulated and 251were down-regulated while during flowering stage and milking stage 349 and 378 transcripts were differentially expressed, respectively. Gene annotation and GO term showed the presence of transcripts involved in metabolic activities associated with uptake and transport of iron and zinc. In the present study, the 83,721 transcripts were also examined for identification of SSRs. A total of 4,327 SSRs were identified with dominance of tri-nucleotide SSRs in comparison to di-nucleotide SSRs. These EST-SSRs can be used in molecular breeding, genetic diversity analysis and determination of heterozygosity of the allelic loci. Information generated will help in gaining insights into iron and zinc metabolism and develop genotypes with high yield, grain iron and zinc content.

Evaluation of pearl millet [Pennisetum glaucum (L.) R. Br.] for grain iron and zinc content in different agro climatic zones of India

2017 ◽  
Vol 77 (1) ◽  
pp. 65 ◽  
Author(s):  
N. Anuradha ◽  
C. Tara Satyavathi ◽  
M. C. Meena ◽  
S. Mukesh Sankar ◽  
C. Bharadwaj ◽  
...  
Keyword(s):  
Pearl Millet ◽  
Pennisetum Glaucum ◽  
Zinc Content ◽  
Climatic Zones ◽  
Iron And Zinc

Potential of non-GMO biofortified pearl millet (Pennisetum glaucum) for increasing iron and zinc content and their estimated bioavailability during abrasive decortication

2012 ◽  
Vol 47 (8) ◽  
pp. 1660-1668 ◽  
Author(s):  
Fatoumata Hama ◽  
Christèle Icard-Vernière ◽  
Jean-Pierre Guyot ◽  
Isabelle Rochette ◽  
Bréhima Diawara ◽  
...  
Keyword(s):  
Pearl Millet ◽  
Pennisetum Glaucum ◽  
Zinc Content ◽  
Iron And Zinc

scholarly journals Genotype by Environment Interaction on Grain Yield Stability and Iron and Zinc Content in OPV of Pearl Millet in Ghana Using the AMMI Method

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Peter Anabire Asungre ◽  
Richard Akromah ◽  
Alexander Wireko Kena ◽  
Prakash Gangashetty
Keyword(s):  
Grain Yield ◽  
Pearl Millet ◽  
Coefficient Of Variation ◽  
Pennisetum Glaucum ◽  
Zinc Content ◽  
Genotype By Environment Interaction ◽  
Phenotypic Variance ◽  
Environment Interaction ◽  
Fe Content ◽  
Iron And Zinc

Twenty-two open-pollinated varieties (OPVs) of pearl millet (Pennisetum glaucum) genotypes were tested in two locations for three seasons in Ghana to estimate the magnitude of genetic variability, heritability, and stability for grain yield and related traits and grain micronutrients among the varieties. General analysis of variance within and across locations and years revealed very highly significant variability ( p < 0.01 ) among the genotypes. The additive main effects and multiplicative interaction (AMMI) analyses revealed significant genotype × environment interaction (GEI) that influenced the relative ranking of genotypes across the environments. Genotypic variance ( σ 2 g ) contributed a greater proportion of the phenotypic variance (σ2p) for plant height (530.31) and grain Fe content (34.72). Broad-sense heritability ( h b s 2 ) varied widely from 24.82% for grain yield to 77.53% in days to flower. Phenotypic coefficient of variation (PCV) was higher than genotypic coefficient of variation (GCV) for all traits, indicating strong play of environment on trait expressions. 11 out of the 22 OPVs were stable for grain yield and micronutrients across environments for the three-year period and included GB 8735 and ICMV 221 Wbr and SOSAT-C88.

scholarly journals Silica Production across Candidate Lignocellulosic Biorefinery Feedstocks

Agronomy ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 82
Author(s):  
Yifeng Xu ◽  
Nick Porter ◽  
Jamie L. Foster ◽  
James P. Muir ◽  
Paul Schwab ◽  
...  
Keyword(s):  
Pearl Millet ◽  
Panicum Virgatum ◽  
Pennisetum Glaucum ◽  
Value Added ◽  
Neutral Detergent Fiber ◽  
Yield Potential ◽  
High Yield ◽  
Energy Cane ◽  
Lignocellulosic Feedstocks ◽  
Sunn Hemp

Biofuels produced from non-food lignocellulosic feedstocks have the potential to replace a significant percentage of fossil fuels via high yield potential and suitability for cultivation on marginal lands. Commercialization of dedicated lignocellulosic crops into single biofuels, however, is hampered by conversion technology costs and decreasing oil prices. Integrated biorefinery approaches, where value-added chemicals are produced in conjunction with biofuels, offer significant potential towards overcoming this economic disadvantage. In this study, candidate lignocellulosic feedstocks were evaluated for their potential biomass and silica yields. Feedstock entries included pearl millet-napiergrass (“PMN”; Pennisetum glaucum [L.] R. Br. × P. purpureum Schumach.), napiergrass (P. purpureum Schumach.), annual sorghum (Sorghum bicolor [L.] Moench), pearl millet (P. glaucum [L.] R. Br.), perennial sorghum (Sorghum spp.), switchgrass (Panicum virgatum L.), sunn hemp (Crotalaria juncea L.), giant miscanthus (Miscanthus × giganteus J.M. Greef and Deuter), and energy cane (Saccharum spp.). Replicated plots were planted at three locations and characterized for biomass yield, chemical composition including hemicellulose, cellulose, acid detergent lignin (ADL), neutral detergent fiber (NDF), crude protein (CP), and silica concentration. The PMN, napiergrass, energy cane, and sunn hemp had the highest biomass yields. They were superior candidates for ethanol production due to high cellulose and hemicellulose content. They also had high silica yield except for sunn hemp. Silica yield among feedstock entries ranged from 41 to 3249 kg ha−1. Based on high bioethanol and biosilica yield potential, PMN, napiergrass, and energy cane are the most promising biorefinery feedstock candidates for improving biofuel profitability.

scholarly journals Descriptive Statistics and Heritability for Agronomic Traits and Grain Micronutrient Content in Rice (Oryza sativa L.)

2019 ◽  
pp. 1-10
Author(s):  
Avinash Kumar ◽  
Ashutosh Kumar ◽  
N. K. Singh ◽  
Rajesh Kumar ◽  
S. K. Singh ◽  
...  
Keyword(s):  
Grain Yield ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Block Design ◽  
Zinc Content ◽  
Yield Potential ◽  
High Yield ◽  
Grain Zinc ◽  
Iron And Zinc ◽  
Breeding Programmes

In the present investigation, 10 parents and their 45 crosses (half diallel) along with 2 standard checks (Rajendra Nilam and Rajendra Mahsuri-1) were evaluated during Kharif, 2018 in Randomized Complete Block Design (RCBD) with 3 replications at Rice Farm Section, Dr. Rajendra Prasad Central Agricultural University (RPCAU), Pusa, Bihar. The objective of this study was to identify promising rice genotypes having desirable combination of morphological traits along with high grain iron and zinc content and high grain yield potential. The results of variability parameters indicated that ample amount of genetic variability was present for all the studied traits. Most of the traits showed high heritability coupled with high genetic advance indicating fruitfulness of selection for improvement of these traits. One genotype (P2×P7) with high grain iron (16.10 ppm) and grain zinc (26.40 ppm) content along with high yield (43.12 g/plant) was identified. Genotypes with high grain iron coupled with high grain yield (P7×P9, P8×P9, P5×P7 and P5×P9) and high  grain zinc content coupled with high grain yield (P4×P7, P9×P10, P8×P9 and P5×P7) were also identified. These promising genotypes identified can be used further in breeding programmes to obtain superior segregants with high grain micronutrient content and high grain yield.

scholarly journals Heterosis and Combining Abilities in a Diverse Seven-Parent Pearl Millet Population Diallel Tested in West Africa

2021 ◽  
Author(s):  
Sonali Dutta ◽  
Felix T. Sattler ◽  
Anna Pucher ◽  
Drabo Inoussa ◽  
Ahmad Issaka ◽  
...  
Keyword(s):  
West Africa ◽  
Pearl Millet ◽  
Combining Ability ◽  
Pennisetum Glaucum ◽  
Smallholder Farmers ◽  
Hybrid Breeding ◽  
High Yield ◽  
Variance Ratios ◽  
High Heritability ◽  
Quantitative Genetic Parameters

Abstract Pearl millet [Pennisetum glaucum (L.) R. Br.] is an important food-security crop to smallholder farmers in West Africa (WA). Breeding for high yield and stability is a major challenge in the harsh environments of WA but could be tackled by hybrid breeding. Knowledge of combining ability patterns and quantitative-genetic parameters is required for an efficient development of hybrid varieties. Hence, our objectives were to estimate the combining ability of seven genetically diverse Sahelian pearl millet populations from Senegal, Mali, Benin, Burkina Faso, Niger, Sudan and Nigeria and the heterosis and stability of their 42 diallel-derived population hybrids to inform pearl millet hybrid breeding. The genotypes were evaluated in six environments in WA in 2007. Grain yield (GY) exhibited an average panmictic mid-parent heterosis (PMPH) of 24%, ranging from -1.51% to 64.69%. General combining ability (GCA) was significant across test environments as reflected by high heritability estimates and high GCA:SCA variance ratios. Thus, early selection for parental per se performance would be rewarding. The parental population from Sudan (IP8679) had strongly negative GCA for GY. Its lack of adaptation contributed to the predominance of additive effects in the present germplasm set. Parental populations PE02987 (Senegal), PE05344 (Mali) and ICMV IS 92222 (Niger) showed large positive GCA for GY. Their offspring, especially PE02987 × PE05344 and Kapelga × ICMV IS 92222, exhibited a high and stable GY across all test environments. Tapping the regional pearl millet genetic diversity seems therefore beneficial for hybrid breeding to increase pearl millet productivity in WA.

Genotype × environment interaction and genetic association of grain iron and zinc content with other agronomic traits in RIL population of pearl millet

2018 ◽  
Vol 69 (11) ◽  
pp. 1092
Author(s):  
Tripti Singhal ◽  
C. Tara Satyavathi ◽  
Aruna Kumar ◽  
S. Mukesh Sankar ◽  
S. P. Singh ◽  
...  
Keyword(s):  
Pearl Millet ◽  
Agronomic Traits ◽  
Pennisetum Glaucum ◽  
Recombinant Inbred Lines ◽  
Selection Index ◽  
Principal Component ◽  
Zinc Content ◽  
Environment Interaction ◽  
Genotype Environment Interaction ◽  
Zn Content

Biofortification of lines of pearl millet (Pennisetum glaucum (L.) R.Br.) with increased iron (Fe) and zinc (Zn) will have great impact because pearl millet is an indispensable component of food and nutritional security of inhabitants of arid and semi-arid regions. The aim of the present study was to assess the stability of Fe and Zn content in recombinant inbred lines (RILs) developed for grain Fe and Zn content, and to use these lines in developing micronutrient-rich pearl millet hybrids. A mapping population consisting of 210 RILs along, with parents and checks, was assessed in three consecutive years (2014–16) under rainfed conditions at the same experimental location in an alpha design with two repetitions. Significant differences were observed in genotype, environment and genotype × environment interaction mean squares for all variables, particularly grain micronutrients. The first two principal components of an interaction principal component analysis cumulatively explained 100% of the total variation; respective contributions of the first and second components were 64.0% and 36.0% for Fe, and 58.1% and 41.9% for Zn. A positive and moderately high correlation (0.696**) between Fe and Zn contents suggests good prospects of simultaneous improvement for both micronutrients. Among the 210 RILs, RIL 69, RIL 186, RIL 191, RIL 149 and RIL 45 were found to be more stable with higher mean micronutrient content, additive main effects and multiplicative interaction stability value (ASV) and genotype selection index (GSI) under rainfed condition. These RILs are promising and can be tested further for their combining ability for yield as well as grain micronutrient content for developing superior biofortified, heterotic pearl millet hybrids.

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