scholarly journals Genome survey and high-resolution genetic map provide valuable genetic resources for Fenneropenaeus chinensis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xianhong Meng ◽  
Qiang Fu ◽  
Sheng Luan ◽  
Kun Luo ◽  
Juan Sui ◽  
...  
Keyword(s):  
High Resolution ◽  
Qtl Mapping ◽  
Association Analysis ◽  
Genetic Map ◽  
Selective Breeding ◽  
Fenneropenaeus Chinensis ◽  
Economic Traits ◽  
Significant Qtls ◽  
Genome Survey ◽  
A Genome

AbstractFenneropenaeus chinensis is one of the most important aquaculture species in China. Research on its genomic and genetic structure not only helps us comprehend the genetic basis of complex economic traits, but also offers theoretical guidance in selective breeding. In the present study, a genome survey sequencing was performed to generate a rough reference genome utilized for groping preliminary genome characteristics and facilitate linkage and quantitative trait locus (QTL) mapping. Linkage mapping was conducted using a reduced-representation sequencing method 2b-RAD. In total, 36,762 SNPs were genotyped from 273 progenies in a mapping family, and a high-resolution linkage map was constructed. The consensus map contained 12,884 markers and spanned 5257.81 cM with an average marker interval of 0.41 cM, which was the first high-resolution genetic map in F. chinensis to our knowledge. QTL mapping and association analysis were carried out in 29 characters including body size, sex and disease resistance. 87 significant QTLs were detected in several traits and they were also evaluated by association analysis. Results of this study provide us valuable suggestions in genetic improvement and breeding of new varieties and also lay a basic foundation for further application of cloning of economic genes in selective breeding program and marker-assisted selection.

scholarly journals A genome-wide SNP-based genetic map and QTL mapping for agronomic traits in Chinese cabbage

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Li Huang ◽  
Yafei Yang ◽  
Fang Zhang ◽  
Jiashu Cao
Keyword(s):  
Qtl Mapping ◽  
Genetic Map ◽  
Chinese Cabbage ◽  
Agronomic Traits ◽  
Genome Wide ◽  
A Genome

QTL mapping for economic traits based on a dense genetic map of cotton with PCR-based markers using the interspecific cross of Gossypium hirsutum × Gossypium barbadense

Euphytica ◽  
2006 ◽  
Vol 153 (1-2) ◽  
pp. 181-197 ◽  
Author(s):  
Dao-Hua He ◽  
Zhong-Xu Lin ◽  
Xian-Long Zhang ◽  
Yi-Chun Nie ◽  
Xiao-Ping Guo ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Gossypium Hirsutum ◽  
Genetic Map ◽  
Interspecific Cross ◽  
Gossypium Barbadense ◽  
Economic Traits

QTL Mapping for Kernel Related Traits Based on a High-Density Genetic Map

2015 ◽  
Vol 41 (10) ◽  
pp. 1510 ◽  
Author(s):  
Wei-Wei QIN ◽  
Yong-Xiang LI ◽  
Chun-Hui LI ◽  
Lin CHEN ◽  
Xun WU ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Genetic Map ◽  
High Density

scholarly journals High-Density Genetic Map Construction and Identification of QTLs Controlling Leaf Abscission Trait in Poncirus trifoliata

2021 ◽  
Vol 22 (11) ◽  
pp. 5723
Author(s):  
Yuan-Yuan Xu ◽  
Sheng-Rui Liu ◽  
Zhi-Meng Gan ◽  
Ren-Fang Zeng ◽  
Jin-Zhi Zhang ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Linkage Map ◽  
Genetic Map ◽  
Expression Patterns ◽  
High Density ◽  
Poncirus Trifoliata ◽  
Leaf Abscission ◽  
Comparative Genomic ◽  
Map Comparison ◽  
Genomic Studies

A high-density genetic linkage map is essential for genetic and genomic studies including QTL mapping, genome assembly, and comparative genomic analysis. Here, we constructed a citrus high-density linkage map using SSR and SNP markers, which are evenly distributed across the citrus genome. The integrated linkage map contains 4163 markers with an average distance of 1.12 cM. The female and male linkage maps contain 1478 and 2976 markers with genetic lengths of 1093.90 cM and 1227.03 cM, respectively. Meanwhile, a genetic map comparison demonstrates that the linear order of common markers is highly conserved between the clementine mandarin and Poncirus trifoliata. Based on this high-density integrated citrus genetic map and two years of deciduous phenotypic data, two loci conferring leaf abscission phenotypic variation were detected on scaffold 1 (including 36 genes) and scaffold 8 (including 107 genes) using association analysis. Moreover, the expression patterns of 30 candidate genes were investigated under cold stress conditions because cold temperature is closely linked with the deciduous trait. The developed high-density genetic map will facilitate QTL mapping and genomic studies, and the localization of the leaf abscission deciduous trait will be valuable for understanding the mechanism of this deciduous trait and citrus breeding.

scholarly journals An FGA Frameshift Variant Associated with Afibrinogenemia in Dachshunds

Genes ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 1065
Author(s):  
Reinhard Mischke ◽  
Julia Metzger ◽  
Ottmar Distl
Keyword(s):  
Protein Sequence ◽  
Sanger Sequencing ◽  
Selective Breeding ◽  
Genome Wide Association Study ◽  
Frameshift Mutation ◽  
Homozygosity Mapping ◽  
Genomic Region ◽  
Severe Bleeding ◽  
Genome Wide ◽  
A Genome

Congenital fibrinogen disorders are very rare in dogs. Cases of afibrinogenemia have been reported in Bernese Mountain, Bichon Frise, Cocker Spaniel, Collie, Lhasa Apso, Viszla, and St. Bernard dogs. In the present study, we examined four miniature wire-haired Dachshunds with afibrinogenemia and ascertained their pedigree. Homozygosity mapping and a genome-wide association study identified a candidate genomic region at 50,188,932–64,187,680 bp on CFA15 harboring FGB (fibrinogen beta chain), FGA (fibrinogen alpha chain), and FGG (fibrinogen gamma-B chain). Sanger sequencing of all three fibrinogen genes in two cases and validation of the FGA-associated mutation (FGA:g.6296delT, NC_006597.3:g.52240694delA, rs1152388481) in pedigree members showed a perfect co-segregation with afibrinogenemia-affected phenotypes, obligate carriers, and healthy animals. In addition, the rs1152388481 variant was validated in 393 Dachshunds and samples from 33 other dog breeds. The rs1152388481 variant is predicted to modify the protein sequence of both FGA transcripts (FGA201:p.Ile486Met and FGA-202:p.Ile555Met) leading to proteins truncated by 306 amino acids. The present data provide evidence for a novel FGA truncating frameshift mutation that is very likely to explain the cases of severe bleeding due to afibrinogenemia in a Dachshund family. This mutation has already been spread in Dachshunds through carriers before cases were ascertained. Genetic testing allows selective breeding to prevent afibrinogenemia-affected puppies in the future.

Construction of high density genetic map and QTL mapping in sorghum × sudangrass

Euphytica ◽  
2021 ◽  
Vol 217 (8) ◽  
Author(s):  
Peng Jin ◽  
Lihua Wang ◽  
Wenjie Zhao ◽  
Jian Zheng ◽  
Yi-Hong Wang ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Genetic Map ◽  
High Density ◽  
Sorghum Sudangrass
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