scholarly journals The transcriptome analysis on urea response mechanism in the process of ergosterol synthesis by Cordyceps cicadae

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Qihui Su ◽  
Zhicai Zhang ◽  
Xiaocui Liu ◽  
Feng Wang
Keyword(s):  
Nitrogen Source ◽  
Gene Transcription ◽  
Transcriptome Analysis ◽  
Regulatory Element ◽  
Fermentation Medium ◽  
Transcription Level ◽  
Go Annotation ◽  
Cordyceps Cicadae ◽  
Gene Transcription Level ◽  
Ergosterol Synthesis

AbstractNitrogen source is required for the growth of Cordyceps cicadae and involved in the regulation of metabolite synthesis. In order to further investigate the regulatory effects of nitrogen sources on the ergosterol synthesis by C. cicadae. We first confirmed that urea could significantly increase the ergosterol synthesis. The transcriptome analysis showed that compared with biomass cultured in the control fermentation medium (CFM), 1340 differentially expressed genes (DEGs) were obtained by Gene Ontology (GO) annotation, and 312 DEGs were obtained by Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation from the biomass cultured in CFM + CO(NH2)2. Urea up-regulated D-3-phosphoglycerate dehydrogenase gene transcription level and down-regulated enolase and L-serine/L-threonine ammonialyase gene transcription level, increased serine synthesis, allosterically activate pyruvate kinase, to promote the synthesis of pyruvate and CH3CO ~ SCOA, the primer of ergosterol; Urea increase the genes transcription related with ergosterol synthesis by up-regulating the steroid regulatory element binding protein gene transcription levels. The transcriptome results were provided by those of qRT-PCR. Collectively, our finding provided valuable insights into the regulatory effect of nitrogen source on the ergosterol synthesis by C. cicadae.

scholarly journals Investigating FGF11 gene transcription level in cancer cells among colorectal cancer patients

2020 ◽  
Vol 30 (1) ◽  
pp. 51-58
Author(s):  
Golshan Khalafian ◽  
Maliheh Entezari ◽  
Maryam Bikhof Torbati ◽  
◽  
◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Gene Transcription ◽  
Transcription Level ◽  
Gene Transcription Level ◽  
Colorectal Cancer Patients

scholarly journals Modeling RNA polymerase competition: the effect of σ-subunit knockout and heat shock on gene transcription level

2011 ◽  
Vol 6 (1) ◽  
pp. 3 ◽  
Author(s):  
Vassily A Lyubetsky ◽  
Oleg A Zverkov ◽  
Lev I Rubanov ◽  
Alexandr V Seliverstov
Keyword(s):  
Heat Shock ◽  
Rna Polymerase ◽  
Gene Transcription ◽  
Transcription Level ◽  
Gene Transcription Level

Genetic effects of an air discharge plasma on Staphylococcus aureus at the gene transcription level

2015 ◽  
Vol 106 (21) ◽  
pp. 213701 ◽  
Author(s):  
Zimu Xu ◽  
Jun Wei ◽  
Jie Shen ◽  
Yuan Liu ◽  
Ronghua Ma ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Gene Transcription ◽  
Discharge Plasma ◽  
Genetic Effects ◽  
Transcription Level ◽  
Gene Transcription Level ◽  
Air Discharge

Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007

2016 ◽  
Vol 51 (12) ◽  
pp. 1904-1910 ◽  
Author(s):  
Sang Jun Lee ◽  
Laxmi Prasad Thapa ◽  
Ju Hun Lee ◽  
Han Suk Choi ◽  
Sung Bong Kim ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Enterobacter Aerogenes ◽  
Transcription Level ◽  
Gene Transcription Level ◽  
Stimulation Of

scholarly journals Influence of Host Gene Transcription Level and Orientation on HIV-1 Latency in a Primary-Cell Model

2011 ◽  
Vol 85 (11) ◽  
pp. 5384-5393 ◽  
Author(s):  
L. Shan ◽  
H.-C. Yang ◽  
S. A. Rabi ◽  
H. C. Bravo ◽  
N. S. Shroff ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Cell Model ◽  
Host Gene ◽  
Primary Cell ◽  
Transcription Level ◽  
Gene Transcription Level ◽  
Hiv 1

Transcriptome analysis of maize reveals potential key genes involved in the response to belowground herbivore Holotrichia parallela larvae feeding

Genome ◽  
2020 ◽  
Vol 63 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Yu Pan ◽  
Shi-wen Zhao ◽  
Xin-long Tang ◽  
Shang Wang ◽  
Xiao Wang ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Defense Mechanisms ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
Expression Profiles ◽  
Go Annotation ◽  
Holotrichia Parallela ◽  
Maize Roots ◽  
Gene Transcription Level ◽  
Maize Plants

The larvae of Holotrichia parallela, a destructive belowground herbivore, causes tremendous damages to maize plants. However, little is known if there are any defense mechanisms in maize roots to defend themselves against this herbivore. In the current research, we carried out RNA-sequencing to investigate the changes in gene transcription level in maize roots after H. parallela larvae infestation. A total of 644 up-regulated genes and 474 down-regulated genes was found. In addition, Gene ontology (GO) annotation analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed. Weighted gene co-expression network analysis (WGCNA) indicated that peroxidase genes may be the hub genes that regulate maize defenses to H. parallela larvae attack. We also found 105 transcription factors, 44 hormone-related genes, and 62 secondary metabolism-related genes within differentially expressed genes (DEGs). Furthermore, the expression profiles of 12 DEGs from the transcriptome analysis were confirmed by quantitative real-time PCR experiments. This transcriptome analysis provides insights into the molecular mechanisms of the underground defense in maize roots to H. parallela larvae attack and will help to select target genes of maize for defense against belowground herbivory.

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