scholarly journals Investigating FGF11 gene transcription level in cancer cells among colorectal cancer patients

2020 ◽  
Vol 30 (1) ◽  
pp. 51-58
Author(s):  
Golshan Khalafian ◽  
Maliheh Entezari ◽  
Maryam Bikhof Torbati ◽  
◽  
◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Gene Transcription ◽  
Transcription Level ◽  
Gene Transcription Level ◽  
Colorectal Cancer Patients

scholarly journals Tumour‐suppressive effect of oestrogen receptor β in colorectal cancer patients, colon cancer cells, and a zebrafish model

2020 ◽  
Vol 251 (3) ◽  
pp. 297-309
Author(s):  
Geriolda Topi ◽  
Shakti Ranjan Satapathy ◽  
Pujarini Dash ◽  
Syrina Fred Mehrabi ◽  
Roy Ehrnström ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Oestrogen Receptor ◽  
Suppressive Effect ◽  
Colon Cancer Cells ◽  
Zebrafish Model ◽  
Colorectal Cancer Patients

scholarly journals HUMAN DNA QUANTIFICATION IN THE STOOLS OF PATIENTS WITH COLORECTAL CANCER

2015 ◽  
Vol 52 (4) ◽  
pp. 293-298 ◽  
Author(s):  
Yolanda TEIXEIRA ◽  
Jacqueline Miranda LIMA ◽  
Maria Luiza Almeida Prado Oliveira SOUZA ◽  
Pedro AGUIAR Jr ◽  
Tiago Donizetti SILVA ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Roc Curve ◽  
Dna Quantification ◽  
Non Invasive ◽  
Human Dna ◽  
The World ◽  
Invasive Method ◽  
Colorectal Cancer Patients

Background - Colorectal cancer is one of the main cause of cancer in the world. Colonoscopy is the best screen method, however the compliance is less than 50%. Quantification of human DNA (hDNA) in the feces may be a possible screen non-invasive method that is a consequence of the high proliferation and exfoliation of cancer cells. Objective - To quantify the human DNA in the stools of patients with colorectal cancer or polyps. Methods - Fifty patients with CRC, 26 polyps and 53 with normal colonoscopy were included. Total and human DNA were analyzed from the frozen stools. Results - An increased concentration of hDNA in the stools was observed in colorectal cancer patients compared to controls and polyps. Tumors localized in the left side of the colon had higher concentrations of hDNA. There were no difference between polyps and controls. A cut off of 0.87 ng/mL of human DNA was determined for colorectal cancer patients by the ROC curve, with a sensitivity of 66% and a specificity of 86.8%. For polyps the cut off was 0.41, the sensitivity was 41% and the specificity 77.4%. Conclusion - A higher concentration of hDNA had been found in colorectal cancer patients The quantification of hDNA from the stools can be a trial method for the diagnosis of colorectal cancer.

The Role of Big Endothelin-1 in Colorectal Cancer

2002 ◽  
Vol 17 (4) ◽  
pp. 268-274 ◽  
Author(s):  
C. Arun ◽  
B. Swift ◽  
K.E. Porter ◽  
K.P. West ◽  
N.J.M. London ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Plasma Concentration ◽  
Liver Metastases ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Hepatic Metastases ◽  
Plasma Samples ◽  
Median Score ◽  
Endothelin 1 ◽  
Colorectal Cancer Patients

Introduction Changes in liver blood flow caused by an unknown splanchnic vasoconstrictor have been noted in colorectal cancer patients with liver metastases. This prospective study was performed to assess whether plasma levels of big endothelin-1 (big ET-1) were raised in patients with colorectal cancer. Methods Plasma samples from peripheral vein of patients who underwent surgery for primary colorectal cancer (n=60) and those with known colorectal liver metastases (n=45) for a period of 15 months were taken prior to treatment and compared to age- and sex-matched controls (n=20). Plasma samples were analysed by using a single-step sandwich enzyme immunoassay. Immunohistochemistry and in situ hybridisation were also performed on tumour sections to investigate the expression of ET-1 by cancer cells. Results The median (range) plasma concentration of big ET-1 in controls was 2.1 pg/mL (1.2–13.4 pg/mL). The median (range) plasma concentration of big ET-1 in colorectal cancer patients with no overt hepatic metastases was 3.8 pg/mL (1.2–15.8 pg/mL), p=0.002, and the median (range) plasma concentration of big ET-1 in colorectal cancer patients with hepatic metastases was 5.2 pg/mL (1.7–30 pg/mL), p=0.0001; both were significantly elevated compared to the control group. A significant difference in immunostaining for big ET-1 was noted between paired normal colonic mucosa (median score-1) and tumour sections (median score-3), p=0.01. Conclusion This study has demonstrated elevated concentrations of big ET-1 in colorectal cancer patients, especially in those with hepatic metastases. Upregulation of ET activity in colorectal cancer could be inferred by the increased immunostaining of big ET-1 in cancer cells. Therefore, plasma big ET-1 levels should be evaluated as a potential tumour marker for the identification of hepatic metastases at an earlier stage.

scholarly journals Improved Activation toward Primary Colorectal Cancer Cells by Antigen-Specific Targeting Autologous Cytokine-Induced Killer Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Claudia Schlimper ◽  
Andreas A. Hombach ◽  
Hinrich Abken ◽  
Ingo G. H. Schmidt-Wolf
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Colon Carcinoma ◽  
Ex Vivo ◽  
Carcinoma Cells ◽  
Adoptive Therapy ◽  
Cik Cells ◽  
Colon Carcinoma Cells ◽  
Colorectal Cancer Patients

Adoptive therapy of malignant diseases with cytokine-induced killer (CIK) cells showed promise in a number of trials; the activation of CIK cells from cancer patients towards their autologous cancer cells still needs to be improved. Here, we generated CIK cellsex vivofrom blood lymphocytes of colorectal cancer patients and engineered those cells with a chimeric antigen receptor (CAR) with an antibody-defined specificity for carcinoembryonic antigen (CEA). CIK cells thereby gained a new specificity as defined by the CAR and showed increase in activation towards CEA+colon carcinoma cells, but less in presence of CEA−cells, indicated by increased secretion of proinflammatory cytokines. Redirected CIK activation was superior by CAR-mediated CD28-CD3ζ than CD3ζ signaling only. CAR-engineered CIK cells from colon carcinoma patients showed improved activation against their autologous, primary carcinoma cells from biopsies resulting in more efficient tumour cell lysis. We assume that adoptive therapy with CAR-modified CIK cells shows improved selectivity in targeting autologous tumour lesions.

Detection of cancer cells in mesenteric and peripheral veins by measuring telomerase activity in colorectal cancer patients

2004 ◽  
Vol 22 (14_suppl) ◽  
pp. 3628-3628
Author(s):  
H. Nozawa ◽  
T. Watanabe ◽  
T. Ohnishi ◽  
T. Tada ◽  
G. Tsurita ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Telomerase Activity ◽  
Colorectal Cancer Patients

scholarly journals Investigation of free cancer cells in peripheral blood using CEA mRNA expression in perioperative colorectal cancer patients

2013 ◽  
Vol 1 (4) ◽  
pp. 668-674 ◽  
Author(s):  
KIICHI NAGAYASU ◽  
HIROMITSU KOMIYAMA ◽  
SHUN ISHIYAMA ◽  
DAI OGURA ◽  
RINA TAKAHASHI ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mrna Expression ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Cea Mrna ◽  
Colorectal Cancer Patients

Upregulation lnc-NEAT1 contributes to colorectal cancer progression through sponging miR-486-5p and activating NR4A1/Wnt/β-catenin pathway

2020 ◽  
pp. 1-11
Author(s):  
Zhining Liu ◽  
Yimei Gu ◽  
Xiaohu Cheng ◽  
Heng Jiang ◽  
Yang Huang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Signaling Pathway ◽  
Cancer Progression ◽  
Luciferase Reporter ◽  
Colorectal Cancer Cells ◽  
Colorectal Cancer Progression ◽  
Colorectal Cancer Patients ◽  
Cancer Tissues

Colorectal cancer is a major public health problem and fourth guiding cause of cancer-induced mortality worldwide. The five-year survival rate for patients with colorectal cancer remains poor, and almost half of colorectal cancer patients present recurrence and die within five years. The increasing studies showed that long non-coding RNA (lncRNA) was involved in colorectal cancer. Therefore, this study was used to explore molecular mechanisms of nuclear paraspeckle assembly transcript 1 (NEAT1) in colorectal cancer. The real-time quantitative polymerase chain reaction (RT-qPCR) was employed to estimate the expression levels of NEAT1, Nuclear receptor 4 A1 (NR4A1), and miR-486-5p in colorectal cancer tissues and cells. Kaplan-Meier curve was conducted to analyze relationship between survival time of colorectal cancer patients and level of NEAT1. The protein levels of NR4A1, β-catenin, c-Myc, and cyclinD1 were assessed with western blot assay. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazol-3-ium bromide (MTT) and flow cytometry assays were performed to evaluate proliferation and apoptosis of colorectal cancer cells, respectively. The migration and invasion abilities of cells were examined by transwell assay. The relationship between miR-486-5p and NEAT1 or NR4A1 was confirmed by dual-luciferase reporter assay. We found NEAT1 and NR4A1 were highly expressed in colorectal cancer tissues and cell lines compared with controls. Loss-functional experiments revealed that knockdown of NEAT1 or NR4A1 repressed proliferation and motility, while inducing apoptosis of colorectal cancer cells. The gain of NR4A1 could abolish NEAT1 silencing-induced effects in colorectal cancer cells. In addition, NEAT1 contributed to colorectal cancer progression through mediating NR4A1/Wnt/β-catenin signaling pathway. In conclusion, NEAT1 stimulated colorectal cancer progression via acting as competing endogenous RNA to sponge miR-486-5p and regulate NR4A1/Wnt/β-catenin signaling pathway.

scholarly journals Detection of metastatic cancer cells in mesentery of colorectal cancer patients

2017 ◽  
Vol 23 (34) ◽  
pp. 6315 ◽  
Author(s):  
Xue-Lai Luo ◽  
Da-Xing Xie ◽  
Jian-Xin Wu ◽  
An-Ding Wu ◽  
Zong-Qing Ge ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Metastatic Cancer ◽  
Colorectal Cancer Patients ◽  
Metastatic Cancer Cells

Detection of cancer cells in mesenteric and peripheral veins by measuring telomerase activity in colorectal cancer patients

2004 ◽  
Vol 22 (14_suppl) ◽  
pp. 3628-3628
Author(s):  
H. Nozawa ◽  
T. Watanabe ◽  
T. Ohnishi ◽  
T. Tada ◽  
G. Tsurita ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Telomerase Activity ◽  
Colorectal Cancer Patients

IL-22 promotes the proliferation of cancer cells in smoking colorectal cancer patients

Tumor Biology ◽  
2015 ◽  
Vol 37 (1) ◽  
pp. 1349-1356 ◽  
Author(s):  
Bao Song ◽  
Yuan Ma ◽  
Xiuchun Liu ◽  
Wanhu Li ◽  
Jianbo Zhang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Colorectal Cancer Patients
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