scholarly journals Proteomic study of Desulfovibrio ferrophilus IS5 reveals overexpressed extracellular multi-heme cytochrome associated with severe microbiologically influenced corrosion

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohor Chatterjee ◽  
Yu Fan ◽  
Fang Cao ◽  
Aaron A. Jones ◽  
Giovanni Pilloni ◽  
...  

AbstractMicrobiologically influenced corrosion (MIC) is recognized as a considerable threat to carbon steel asset integrity in the oil and gas industry. There is an immediate need for reliable and broadly applicable methods for detection and monitoring of MIC. Proteins associated with microbial metabolisms involved in MIC could serve as useful biomarkers for MIC diagnosis and monitoring. A proteomic study was conducted using a lithotrophically-grown bacterium Desulfovibrio ferrophilus strain IS5, which is known to cause severe MIC in seawater environments. Unique proteins, which are differentially and uniquely expressed during severe microbial corrosion by strain IS5, were identified. This includes the detection of a multi-heme cytochrome protein possibly involved in extracellular electron transfer in the presence of the carbon steel. Thus, we conclude that this newly identified protein associated closely with severe MIC could be used to generate easy-to-implement immunoassays for reliable detection of microbiological corrosion in the field.

Author(s):  
Lisa M. Gieg ◽  
Mohita Sharma ◽  
Trevor Place ◽  
Jennifer Sargent ◽  
Yin Shen

Abstract Corrosion of carbon steel infrastructure in the oil and gas industry can occur via a variety of chemical, physical, and/or microbiological mechanisms. Although microbial corrosion is known to lead to infrastructure failure in many upstream and downstream operations, predicting when and how microorganisms attack metal surfaces remains a challenge. In crude oil transmission pipelines, a kind of aggressive corrosion known as under deposit corrosion (UDC) can occur, wherein mixtures of solids (sands, clays, inorganic minerals), water, oily hydrocarbons, and microorganisms form discreet, (bio)corrosive sludges on the metal surface. To prevent UDC, operators will use physical cleaning methods (e.g., pigging) combined with chemical treatments such as biocides, corrosion inhibitors, and/or biodispersants. As such, it necessary to evaluate the efficacy of these treatments in preventing UDC by monitoring the sludge characteristics and the microorganisms that are potentially involved in the corrosion process. The efficacies of a biocide, corrosion inhibitor, and biodispersant being used to prevent microbial corrosion in a crude oil transmission pipeline were evaluated. A combination of various microbiological analyses and corrosivity tests were performed using sludge samples collected during pigging operations. The results indicated that the combined treatment using inhibitor, biocide 1 and biodispersant was the most effective in preventing metal damage, and both growth-based and Next-Generation Sequencing approaches provided value towards understanding the effects of the chemical treatments. The efficacy of a different biocide (#2) could be discriminated using these test methods. The results of this study demonstrate the importance of considering and monitoring for microbial corrosion of crucial metal infrastructure in the oil and gas industry, and the value of combining multiple lines of evidence to evaluate the performance of different chemical treatment scenarios.


2015 ◽  
Vol 74 (4) ◽  
Author(s):  
M. K. F. M. Ali ◽  
N. Md. Noor ◽  
N. Yahaya ◽  
A. A. Bakar ◽  
M. Ismail

Pipelines play an extremely important role in the transportation of gases and liquids over long distance throughout the world. Internal corrosion due to microbiologically influenced corrosion (MIC) is one of the major integrity problems in oil and gas industry and is responsible for most of the internal corrosion in transportation pipelines. The presence of microorganisms such as sulfate reducing bacteria (SRB) in pipeline system has raised deep concern within the oil and gas industry. Biocide treatment and cathodic protection are commonly used to control MIC. However, the solution is too expensive and may create environmental problems by being too corrosive. Recently, Ultraviolet (UV) as one of the benign techniques to enhance mitigation of MIC risk in pipeline system has gained interest among researchers. An amount of 100 ml of modified Baar’s medium and 5 ml of Desulfovibrio vulgaris (strain 7577) seeds was grown in 125 ml anaerobic vials with carbon steel grade API 5L-X70 coupons at the optimum temperature of 37°C and pH 9.5 for fifteen days. This was then followed by exposing the medium to UV for one hour. Results from present study showed that UV radiation has the ability to disinfect bacteria, hence minimizing the risk of metal loss due to corrosion in steel pipeline. 


Author(s):  
Thilo Reichel ◽  
Jochem Beissel ◽  
Vitaliy Pavlyk ◽  
Gernot Heigl

The paper describes the different industrially used options to produce a clad pipe and explains in detail the manufacture of metallurgically cladded pipes starting with the production of roll bonded plates. In plate manufacturing the advantages as well as the limitations of thermo-mechanical (TM) rolling are discussed. The TM-technology is shown to improve weldability, HIC-resistance, strength and toughness properties of the carbon steel section of the pipe. Moreover, it also improves corrosion resistance of the CRA layer. The pipe manufacturing procedure, which involves two welding technologies for longitudinal welds is described. The carbon steel parts of the pipe are joined using double-sided multi-pass Submerged-Arc-Welding (SAW). The single-pass Electroslag-Welding (ESW) is subsequently used for recladding of the CRA layer. The multi-pass SAW results in excellent mechanical properties of the weld joint, whereas the ESW technique ensures low dilution of CRA with the carbon steel, a smooth weld bead shape and a high corrosion resistance of the deposited layer. With the aid of thermodynamic modeling and numerical simulations it is shown, that the high corrosion resistance is promoted by an intensive mixing within the ESW weld pool and relatively low segregation level of Cr and Mo during solidification. Furthermore, FEM analysis is applied to examine the plastic deformation and residual stresses distribution in the pipe during forming, welding and final calibration. The obtained information assists in optimization of manufacturing procedure, and can also be included in prediction of resulting pipe fatigue during operation.


2019 ◽  
Vol 3 (1) ◽  
pp. 30-36
Author(s):  
Zuraini Din ◽  

In the oil and gas industry, pipeline is the major transportation medium to deliver the products. According to [1] containment of pipeline loss to indicate that corrosion has been found to be the most predominant cause for failures of buried metal pipes. MIC has been identified as one of the major causes of underground pipeline corrosion failure and Sulphate Reducing Bacteria (SRB) are the main reason causing MIC, by accelerating corrosion rate. The objectives of this study is to study the SRB growth, Desulfovibrio desulfuricans ATCC 7757 due to pH and determine the optimum value controlling the bacteria growth on the internal pipe of carbon steel grade API X70. The result shows that the optimum SRB growth is at range pH 5-5 to 6.5 and the exposure time of 7 to 14 days. At pH 6.5 the maximum corrosion rate is 1.056 mm/year. Corrosion phenomena on carbon steel in the study proven had influence by pH and time. From this result pitting corrosion strongly attack at carbon steel pipe. In the future project, it is recommended to study the effect of different pipe location for example the pipeline under seawater.


2021 ◽  
Author(s):  
Chinedu Oragwu ◽  
Daniel Molyneux ◽  
Lukeman Lawal ◽  
Stanley Ameh

Abstract Carbon steel pipelines are used to transport hydrocarbons globally because carbon steel is relatively easier to fabricate, safe for use, raw materials are available and less expensive. Amidst these benefits, carbon steel is susceptible to severe corrosion and other anomalies. Pipeline corrosion is a significant concern in the oil and gas industry. It has caused several minor and catastrophic losses of containment with resultant fatalities, environmental pollutions, asset damage, and production downtimes. The increasing failures of in-service pipelines have led the Department of Petroleum Resources (DPR) to intensify regulatory scrutiny of pipeline integrity assessment and management in Nigeria to ensure strict compliance to the regulatory requirements by the Oil Producing Companies. According to DPR Act (Section 2.5.2.1), all pipelines greater than 6" size diameter must be inspected every five (5) years with intelligent pigs (inline inspection tools) that would provide the accurate condition of the pipeline. However, many pipelines in Nigeria are unpiggable or difficult to inspect with intelligent pigs due to the unavailability of pigging facilities (especially in brownfields), pipelines with short bend radiuses, dual diameters, flow parameters, etcetera. This paper explores case studies involving the use of advanced inline inspection technology to conduct inline inspection of difficult-to-inspect dual-diameter pipelines.


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