scholarly journals Two Nicotiana occidentalis accessions enable gene identification for Type II hybrid lethality by the cross to N. sylvestris

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kenji Kawaguchi ◽  
Yuichiro Ohya ◽  
Maho Maekawa ◽  
Takahiro Iizuka ◽  
Akira Hasegawa ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Plant Species ◽  
Single Gene ◽  
Gene Identification ◽  
Type Ii ◽  
Hybrid Lethality ◽  
The Cross ◽  
Allelic Interaction

AbstractHybrid lethality, meaning the death of F1 hybrid seedlings, has been observed in many plant species, including Nicotiana. Previously, we have revealed that hybrids of the selected Nicotiana occidentalis accession and N. tabacum, an allotetraploid with S and T genomes, exhibited lethality characterized by the fading of shoot color. The lethality was suggested to be controlled by alleles of loci on the S and T genomes derived from N. sylvestris and N. tomentosiformis, respectively. Here, we extended the analysis of hybrid lethality using other two accessions of N. occidentalis identified from the five tested accessions. The two accessions were crossed with N. tabacum and its two progenitors, N. sylvestris and N. tomentosiformis. After crosses with N. tabacum, the two N. occidentalis accessions yielded inviable hybrid seedlings whose lethality was characterized by the fading of shoot color, but only the T genome of N. tabacum was responsible for hybrid lethality. Genetic analysis indicated that first-mentioned N. occidentalis accession carries a single gene causing hybrid lethality by allelic interaction with the S genome.

scholarly journals GENETIC ANALYSIS OF FAMILIES HAVING AUTOSOMAL RECESSIVE INTELLECTUAL DISABILITY

2019 ◽  
Vol 17 (2) ◽  
Author(s):  
Jamshed Khan ◽  
Muhammad Junaid ◽  
Shahab Uddin ◽  
Khalida Moeed ◽  
Usman Ullah ◽  
...  
Keyword(s):  
Intellectual Disability ◽  
Genetic Analysis ◽  
Single Gene ◽  
Cross Sectional Study ◽  
Molecular Diagnostic ◽  
Developmental Defect ◽  
Chromosomal Disorders ◽  
Cross Sectional ◽  
Pathogenic Variants ◽  
Development Delay

Background: Intellectual disability (ID) is a neuro-developmental defect that is manifested by development delay and learning disability. Such defects may be caused due to chromosomal disorders (trisomy 18 or Down syndrome) or single gene mutation. Its worldwide prevalence is estimated to be 1-3%. The genetic etiology of non-syndromic ID is poorly understood. To date, more than 100 loci have been reported to be associated with non-syndromic ID. The objective of this study was to identify the causative genes for three Materials & Methods: This cross-sectional study was conducted in the Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan from March 2014 to August 2015. The inclusion criteria set for the families was consanguineous relation and more than two patients per family (including cousins). All the patients were tested individually in friendly atmosphere using IQ test to scale the ID on the basis of performance. Thereafter, blood samples were taken by aseptic method and DNA was extracted for the purpose of doing genetic analysis. In genetic analysis, exome sequencing was performed to find the pathogenic variants. Subsequently. Sanger sequencing was also done to see the segregation of pathogenic variants. Results: Genetic analysis found mutation in AP4B1 in Family 1, in WDR62 in Family 2, while Family 3 was unremarkable. Conclusion: The study involved genetic analysis of three consanguineous families and found mutation in AP4B1 in Family 1, in WDR62 in Family 2, while Family 3 was unremarkable. The present research will help in devising molecular diagnostic technics for pre-marital and pre-conception testing.

scholarly journals Genetic Analysis of CLCN7 in an Old Female Patient with Type II Autosomal Dominant Osteopetrosis

2018 ◽  
Vol 33 (3) ◽  
pp. 380 ◽  
Author(s):  
Seon Young Kim ◽  
Younghak Lee ◽  
Yea Eun Kang ◽  
Ji Min Kim ◽  
Kyong Hye Joung ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Female Patient ◽  
Autosomal Dominant ◽  
Type Ii ◽  
Autosomal Dominant Osteopetrosis

scholarly journals Correction to: Clinical and genetic analysis of two wolfram syndrome families with high occurrence of wolfram syndrome and diabetes type II: a case report

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Maryam Sobhani ◽  
Mohammad Amin Tabatabaiefar ◽  
Soudeh Ghafouri-Fard ◽  
Asadollah Rajab ◽  
Asal Hojjat ◽  
...  
Keyword(s):  
Case Report ◽  
Genetic Analysis ◽  
Wolfram Syndrome ◽  
Diabetes Type ◽  
Type Ii ◽  
High Occurrence ◽  
Diabetes Type Ii

Identification and characterization of p63 (CKAP4/ERGIC-63/CLIMP-63), a surfactant protein A binding protein, on type II pneumocytes

2006 ◽  
Vol 291 (3) ◽  
pp. L436-L446 ◽  
Author(s):  
Nisha Gupta ◽  
Yefim Manevich ◽  
Altaf S. Kazi ◽  
Jian-Qin Tao ◽  
Aron B. Fisher ◽  
...  
Keyword(s):  
Binding Protein ◽  
Protein A ◽  
Surfactant Protein ◽  
Surfactant Protein A ◽  
Type Ii Cells ◽  
Receptor Complex ◽  
Type Ii ◽  
Surface Binding ◽  
Intact Mouse ◽  
The Cross

Surfactant protein A (SP-A) binds to alveolar type II cells through a specific high-affinity cell membrane receptor, although the molecular nature of this receptor is unclear. In the present study, we have identified and characterized an SP-A cell surface binding protein by utilizing two chemical cross-linkers: profound sulfo-SBED protein-protein interaction reagent and dithiobis(succinimidylpropionate) (DSP). Sulfo-SBED-biotinylated SP-A was cross-linked to the plasma membranes isolated from rat type II cells, and the biotin label was transferred from SP-A to its receptor by reduction. The biotinylated SP-A-binding protein was identified on blots by using streptavidin-labeled horseradish peroxidase. By using DSP, we cross-linked SP-A to intact mouse type II cells and immunoprecipitated the SP-A-receptor complex using anti-SP-A antibody. Both of the cross-linking approaches showed a major band of 63 kDa under reduced conditions that was identified as the rat homolog of the human type II transmembrane protein p63 (CKAP4/ERGIC-63/CLIMP-63) by matrix-assisted laser desorption ionization and nanoelectrospray tandem mass spectrometry of tryptic fragments. Thereafter, we confirmed the presence of p63 protein in the cross-linked SP-A-receptor complex by immunoprobing with p63 antibody. Coimmunoprecipitation experiments and functional assays confirmed specific interaction between SP-A and p63. Antibody to p63 could block SP-A-mediated inhibition of ATP-stimulated phospholipid secretion. Both intracellular and membrane localized pools of p63 were detected on type II cells by immunofluorescence and immunobloting. p63 colocalized with SP-A in early endosomes. Thus p63 closely interacts with SP-A and may play a role in the trafficking or the biological function of the surfactant protein.

THE INHERITANCE OF MATURITY IN MAIZE

1960 ◽  
Vol 40 (3) ◽  
pp. 490-499 ◽  
Author(s):  
John Giesbrecht
Keyword(s):  
Genetic Analysis ◽  
Genetic Model ◽  
Individual Plant ◽  
Effective Factors ◽  
Basic Assumptions ◽  
Heritability Estimates ◽  
The Cross ◽  
Plant Basis

Days from seeding to silking and days from seeding to pollen shedding were used as measures of maturity on the parents, their F1, F2 and the back-crosses of the F1 to both parents of the cross Mt42 × WF9. Readings were taken on an individual plant basis. Results indicated the existence of partial phenotypic dominance for earliness. Heritability estimates were very low, probably due to the lack of agreement between the hereditary behaviour of the two characters and the basic assumptions of the formula used. A genetic analysis by the partitioning method of the two characters, days to silking and days to pollen shedding, indicated that Mt42 and WF9 differed by four effective factors and that all of the factors for increased earliness were located in the Mt42 line. A genetic model was constructed which appeared to satisfy the data for both characters.

scholarly journals Collapsing-based and kernel-based single-gene analyses applied to Genetic Analysis Workshop 17 mini-exome data

2011 ◽  
Vol 5 (S9) ◽  
Author(s):  
Lun Li ◽  
Wei Zheng ◽  
Joon Sang Lee ◽  
Xianghua Zhang ◽  
John Ferguson ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Genetic Analysis Workshop ◽  
Single Gene ◽  
Exome Data

scholarly journals Are Dietary Lectins Relevant Allergens in Plant Food Allergy?

Foods ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1724
Author(s):  
Annick Barre ◽  
Els J.M. Van Damme ◽  
Mathias Simplicien ◽  
Hervé Benoist ◽  
Pierre Rougé
Keyword(s):  
Plant Species ◽  
Fruits And Vegetables ◽  
Rubber Tree ◽  
Type Ii ◽  
Food Allergens ◽  
Edible Plant ◽  
Ribosome Inactivating Proteins ◽  
Pathogenesis Related Protein ◽  
Legume Lectins ◽  
Potential Food

Lectins or carbohydrate-binding proteins are widely distributed in seeds and vegetative parts of edible plant species. A few lectins from different fruits and vegetables have been identified as potential food allergens, including wheat agglutinin, hevein (Hev b 6.02) from the rubber tree and chitinases containing a hevein domain from different fruits and vegetables. However, other well-known lectins from legumes have been demonstrated to behave as potential food allergens taking into account their ability to specifically bind IgE from allergic patients, trigger the degranulation of sensitized basophils, and to elicit interleukin secretion in sensitized people. These allergens include members from the different families of higher plant lectins, including legume lectins, type II ribosome-inactivating proteins (RIP-II), wheat germ agglutinin (WGA), jacalin-related lectins, GNA (Galanthus nivalis agglutinin)-like lectins, and Nictaba-related lectins. Most of these potentially active lectin allergens belong to the group of seed storage proteins (legume lectins), pathogenesis-related protein family PR-3 comprising hevein and class I, II, IV, V, VI, and VII chitinases containing a hevein domain, and type II ribosome-inactivating proteins containing a ricin B-chain domain (RIP-II). In the present review, we present an exhaustive survey of both the structural organization and structural features responsible for the allergenic potency of lectins, with special reference to lectins from dietary plant species/tissues consumed in Western countries.

Genetic Analysis of Corn Kernel Chemical Composition in the Random Mated 7 Generation of the Cross of Generations 70 of IHP × ILP

Crop Science ◽  
2007 ◽  
Vol 47 (1) ◽  
pp. 45-57 ◽  
Author(s):  
J. W. Dudley ◽  
Darryl Clark ◽  
Torbert R. Rocheford ◽  
John R. LeDeaux
Keyword(s):  
Chemical Composition ◽  
Genetic Analysis ◽  
Corn Kernel ◽  
The Cross

Preimplantation Diagnosis of Single Gene Disorders by Two-Step Oocyte Genetic Analysis Using First and Second Polar Body

1997 ◽  
Vol 62 (2) ◽  
pp. 182-187 ◽  
Author(s):  
Yury Verlinsky ◽  
Svetlana Rechitsky ◽  
Jeanine Cieslak, Victor Ivakhnenko ◽  
George Wolf ◽  
Aaron Lifchez ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Single Gene ◽  
Polar Body ◽  
Preimplantation Diagnosis ◽  
Single Gene Disorders ◽  
Second Polar Body

Genetic and morphological analysis of floral homeotic mutants tepal-like bract and fagopyrum apetala of Fagopyrum esculentum

Botany ◽  
2008 ◽  
Vol 86 (4) ◽  
pp. 367-375 ◽  
Author(s):  
Maria D. Logacheva ◽  
Ivan N. Fesenko ◽  
Aleksey N. Fesenko ◽  
Aleksey A. Penin
Keyword(s):  
Plant Species ◽  
Morphological Analysis ◽  
Morphological Evolution ◽  
Single Gene ◽  
Gene Mutations ◽  
Fagopyrum Esculentum ◽  
Wide Range ◽  
Fagopyrum Esculentum Moench ◽  
Mutant Phenotypes ◽  
Floral Homeotic

The studies on floral homeotic mutants of the model plant species Arabidopsis thaliana (L.) Heynh. and Antirrhinum majus L. have clarified many important aspects of the genetic control of flower development. However, the details of this process can vary in species representing different lineages of flowering plants. The studies on floral homeotic mutants of nonmodel plant species may significantly improve the understanding of the mechanisms of morphological evolution of flowers. We report here the results of the genetic and morphological analysis of two floral homeotic mutants of common buckwheat ( Fagopyrum esculentum Moench.). The mutant, tepal-like bract (tlb), is characterized by the transformation of bracts into petaloid organs, whereas fagopyrum apetala (fap), has a carpelloid perianth. Both mutant phenotypes are caused by a single recessive nuclear mutation. The double mutant fap tlb combines the features of tlb and fap. Our results show that single gene mutations are sufficient to convert the buckwheat bract into a tepal and to confer carpel identity on first whorl organs. These results are consistent with the premise that variations on the ABC model can be used to explain a wide range of floral architectures.

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