Essential oils of Bursera morelensis and Lippia graveolens for the development of a new biopesticides in postharvest control

AbstractFruit and vegetable crops that are not consumed immediately, unlike other agricultural products, require economic and time investments until they reach the final consumers. Synthetic agrochemicals are used to maintain and prolong the storage life of crops and avoid losses caused by phytopathogenic microorganisms. However, the excessive use of synthetic agrochemicals creates health problems and contributes to environmental pollution. To avoid these problems, less toxic and environment-friendly alternatives are sought. One of these alternatives is the application of biopesticides. However, few biopesticides are currently used. In this study, the biopesticide activity of Bursera morelensis and Lippia graveolens essential oils was evaluated. Their antifungal activity has been verified in an in vitro model, and chemical composition has been determined using gas chromatography-mass spectrometry. Their antifungal activity was corroborated in vitro, and their activity as biopesticides was subsequently evaluated in a plant model. In addition, the persistence of these essential oils on the surface of the plant model was determined. Results suggest that both essential oils are promising candidates for producing biopesticides. This is the first study showing that B. morelensis and L. graveolens essential oils work by inhibiting mycelial growth and spore germination and are environment-friendly biopesticides.

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Novel Nystatin A1 derivatives exhibiting low host cell toxicity and antifungal activity in an in vitro model of oral candidosis

Medical Microbiology and Immunology ◽

10.1007/s00430-014-0343-4 ◽

2014 ◽

Vol 203 (5) ◽

pp. 341-355 ◽

Cited By ~ 7

Author(s):

Joanna Boros-Majewska ◽

Natalia Salewska ◽

Edward Borowski ◽

Sławomir Milewski ◽

Sladjana Malic ◽

...

Keyword(s):

Antifungal Activity ◽

Host Cell ◽

In Vitro Model ◽

Cell Toxicity ◽

Oral Candidosis ◽

Vitro Model

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Effect of the Chemical Composition of Free-Terpene Hydrocarbons Essential Oils on Antifungal Activity

Molecules ◽

10.3390/molecules24193532 ◽

2019 ◽

Vol 24 (19) ◽

pp. 3532

Author(s):

Ben Salha ◽

Herrera Díaz ◽

Lengliz ◽

Abderrabba ◽

Labidi

Keyword(s):

Chemical Composition ◽

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

Pharmaceutical Products ◽

Gas Chromatography Mass Spectrometry ◽

Reduced Pressure ◽

Oxygenated Compounds ◽

Food Spoilage Fungi

In this study, Carum carvi L. essential oil (CEO) and Origanum majorana L. essential oil (MEO) was steam-distillated under reduced pressure. We henceforth obtained three fractions for each essential oil: CF1, CF2, CF3, MF1, MF2, and MF3. Then, these fractions were characterized using the gas chromatography–mass spectrometry (GC-MS) technique. The results indicated that some fractions were rich in oxygenated compounds (i.e., CF2, CF3, MF2, and MF3) with concentrations ranging from 79.21% to 98.56%. Therefore, the influence of the chemical composition of the essential oils on their antifungal activity was studied. For this purpose, three food spoilage fungi were isolated, identified, and inoculated in vitro, in order to measure the antifungal activity of CEO, MEO, and their fractions. The results showed that stronger fungi growth inhibitions (FGI) (above 95%) were found in fractions with higher percentages of oxygenated compounds, especially with (−)-carvone and terpin-4-ol as the major components. Firstly, this work reveals that the free-terpenes hydrocarbons fractions obtained from MEO present higher antifungal activity than the raw essential oil against two families of fungi. Then, it suggests that the isolation of (−)-carvone (97.15 ± 5.97%) from CEO via vacuum distillation can be employed successfully to improve antifungal activity by killing fungi (FGI = 100%). This study highlights that separation under reduced pressure is a simple green method to obtain fractions or to isolate compounds with higher biological activity useful for pharmaceutical products or natural additives in formulations.

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Antifungal Activity and Chemical Composition of Seven Essential Oils to Control the Main Seedborne Fungi of Cucurbits

Antibiotics ◽

10.3390/antibiotics10020104 ◽

2021 ◽

Vol 10 (2) ◽

pp. 104

Author(s):

Marwa Moumni ◽

Gianfranco Romanazzi ◽

Basma Najar ◽

Luisa Pistelli ◽

Hajer Ben Amara ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

In Vivo Studies ◽

Gas Chromatography Mass Spectrometry ◽

Major Constituent ◽

Mycelia Growth ◽

Seedborne Fungi ◽

Seedborne Pathogens

Essential oils represent novel alternatives to application of synthetic fungicides to control against seedborne pathogens. This study investigated seven essential oils for in vitro growth inhibition of the main seedborne pathogens of cucurbits. Cymbopogon citratus essential oil completely inhibited mycelial growth of Stagonosporopsis cucurbitacearum and Alternaria alternata at 0.6 and 0.9 mg/mL, respectively. At 1 mg/mL, Lavandula dentata, Lavandula hybrida, Melaleuca alternifolia, Laurus nobilis, and two Origanum majorana essential oils inhibited mycelia growth of A. alternata by 54%, 71%, 68%, 36%, 90%, and 74%, respectively. S. cucurbitacearum mycelia growth was more sensitive to Lavandula essential oils, with inhibition of ~74% at 1 mg/mL. To determine the main compounds in these essential oils that might be responsible for this antifungal activity, they were analyzed by gas chromatography–mass spectrometry (GC-MS). C. citratus essential oil showed cirtal as its main constituent, while L. dentata and L. nobilis essential oils showed eucalyptol. The M. alternifolia and two O. majorana essential oils had terpinen-4-ol as the major constituent, while for L. hybrida essential oil, this was linalool. Thus, in vitro, these essential oils can inhibit the main seedborne fungi of cucurbits, with future in vivo studies now needed to confirm these activities.

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Corrigendum: Stojkovic D, Glamoclija J, Ciric A, Nikolic M, Ristic M, Siljegovic J, Sokovic M. Investigation on antibacterial synergism of Origanum vulgare and Thymus vulgaris essential oils. Arch biol sci. 2013;65(2):639-43. DOI: 10.2298/ABS1302639S

Archives of Biological Sciences ◽

10.2298/abs150713085e ◽

2015 ◽

Vol 67 (4) ◽

pp. 1441-1441

Author(s):

E Editorial

Keyword(s):

Essential Oils ◽

In Vitro Model ◽

Medicinal Herbs ◽

Thymus Vulgaris ◽

Antibacterial Effects ◽

Origanum Vulgare ◽

Vitro Model ◽

Font Color

The authors of the above-referenced article have requested a corrigendum to it. The article should include the following reference: Sokovic, M., Glamoclija, J., Marin, D.P., Brkic, D., and L. J. L. D. van Griensven (2010) Antibacterial Effects of the Essential Oils of Commonly Consumed Medicinal Herbs Using an In Vitro Model. Molecules. 15(11), 7532-7546. Link to the corrected article <a href="http://dx.doi.org/10.2298/ABS1302639S">10.2298/ABS1302639S</a>

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Biopharmaceutical and antifungal properties of ellagic acid-cyclodextrin using an in vitro model of invasive candidiasis

Future Microbiology ◽

10.2217/fmb-2019-0107 ◽

2019 ◽

Vol 14 (11) ◽

pp. 957-967 ◽

Cited By ~ 1

Author(s):

Aline VL Gontijo ◽

Aline da G Sampaio ◽

Cristiane Y Koga-Ito ◽

Marcos J Salvador

Keyword(s):

Antifungal Activity ◽

Ellagic Acid ◽

In Vitro Model ◽

Water Solubility ◽

Antifungal Properties ◽

Characterization Studies ◽

Vitro Model ◽

Biopharmaceutical Properties ◽

Cells Cultured

Aim: To investigate biopharmaceutical and antifungal properties of pure and complexed ellagic acid. Materials & methods: Caco-2 cells cultured in a Transwell® inserts were infected with Candida albicans to develop an in vitro model. Ellagic acid was complexed with cyclodextrins. Microbial compositions, ellagic acid concentration as function of time and characterization studies of complexes were evaluated. Results: Ellagic acid presented ability to reduce C. albicans invasion, although this was not statistically significant. Its poor water solubility and absorption probably limited this ability. Water solubility was increased after complexation with hydroxypropyl-β-CD; however, ellagic acid/hydroxypropyl-β-CD did not improve the antifungal activity. Conclusion: Although ellagic acid presented a promising antifungal activity, its biopharmaceutical properties limit such activity and should be improved.

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Antibacterial Effects of the Essential Oils of Commonly Consumed Medicinal Herbs Using an In Vitro Model

Molecules ◽

10.3390/molecules15117532 ◽

2010 ◽

Vol 15 (11) ◽

pp. 7532-7546 ◽

Cited By ~ 268

Author(s):

Marina Soković ◽

Jasmina Glamočlija ◽

Petar D. Marin ◽

Dejan Brkić ◽

Leo J. L. D. van Griensven

Keyword(s):

Essential Oils ◽

In Vitro Model ◽

Medicinal Herbs ◽

Antibacterial Effects ◽

Vitro Model

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Antimicrobial Effects of Essential Oils on Oral Microbiota Biofilms: The Toothbrush In Vitro Model

Antibiotics ◽

10.3390/antibiotics10010021 ◽

2020 ◽

Vol 10 (1) ◽

pp. 21

Author(s):

Andreia Aires ◽

António Salvador Barreto ◽

Teresa Semedo-Lemsaddek

Keyword(s):

Essential Oil ◽

Essential Oils ◽

In Vitro Model ◽

Inhibitory Concentration ◽

Oral Bacteria ◽

Bacterial Biofilm ◽

Antimicrobial Effects ◽

Vitro Model ◽

Biofilm Development

The present investigation intended to evaluate the bacteriostatic and bactericidal abilities of clove, oregano and thyme essential oils against oral bacteria in planktonic and biofilm states. Furthermore, aiming to mimic everyday conditions, a toothbrush in vitro model was developed. Determination of the minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration and minimum biofilm eradication concentration were achieved using the microdilution procedure. To simulate the toothbrush environment, nylon fibers were inoculated with oral bacteria, which, after incubation to allow biofilm development, were submitted to contact with the essential oils under study. Thyme and oregano essential oils revealed promising antimicrobial effects, both in growth inhibition and the destruction of cells in planktonic and biofilm states, while clove essential oil showed a weaker potential. Regarding the toothbrush in vitro model, observation of the nylon fibers under a magnifying glass proved the essential oil anti-biofilm properties. Considering the effects observed using the in vitro toothbrush model, a realistic approximation to oral biofilm establishment in an everyday use object, a putative application of essential oils as toothbrush sanitizers to help prevent the establishment of bacterial biofilm can be verified.

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An in vitro model for investigation of vitamin A effects on wound healing

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000692 ◽

2021 ◽

pp. 1-6

Author(s):

Hoda Keshmiri Neghab ◽

Mohammad Hasan Soheilifar ◽

Gholamreza Esmaeeli Djavid

Keyword(s):

Wound Healing ◽

Endothelial Cell ◽

Vitamin A ◽

In Vitro Model ◽

Cellular Proliferation ◽

Endothelial Cell Migration ◽

Normal Fibroblast ◽

Anti Inflammatory ◽

Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inﬂammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inﬂammation responses.

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