scholarly journals Effects of ligand and thyroid hormone receptor isoforms on hepatic gene expression profiles of thyroid hormone receptor knockout mice

EMBO Reports ◽  
2003 ◽  
Vol 4 (6) ◽  
pp. 581-587 ◽  
Author(s):  
Paul M Yen ◽  
Xu Feng ◽  
Frederic Flamant ◽  
Yidong Chen ◽  
Robert L Walker ◽  
...  
Endocrinology ◽  
2004 ◽  
Vol 145 (3) ◽  
pp. 1386-1391 ◽  
Author(s):  
Beatriz Morte ◽  
Jimena Manzano ◽  
Thomas S. Scanlan ◽  
Björn Vennström ◽  
Juan Bernal

2020 ◽  
Author(s):  
Shahan Mamoor

Ovarian cancer is the most lethal gynecologic cancer (1). We sought to identify genes associated with high-grade serous ovarian cancer (HGSC) by comparing global gene expression profiles of normal ovary with that of primary tumors from women diagnosed with HGSC using published microarray data (2, 3). We found significant differential expression of the gene encoding the thyroid hormone receptor, THRA, also known as nuclear receptor subfamily 1, group A, member 1 (NR1A1) in high-grade serous ovarian tumors.


2012 ◽  
Vol 142 (5) ◽  
pp. S-988
Author(s):  
Satoru Kakizaki ◽  
Hiroki Tojima ◽  
Yuichi Yamazaki ◽  
Daichi Takizawa ◽  
Norio Horiguchi ◽  
...  

1991 ◽  
Vol 11 (10) ◽  
pp. 5079-5089 ◽  
Author(s):  
D E Banker ◽  
J Bigler ◽  
R N Eisenman

The c-erbA proto-oncogene encodes the thyroid hormone receptor, a ligand-dependent transcription factor which plays an important role in vertebrate growth and development. To define the role of the thyroid hormone receptor in developmental processes, we have begun studying c-erbA gene expression during the ontogeny of Xenopus laevis, an organism in which thyroid hormone has well-documented effects on morphogenesis. Using polymerase chain reactions (PCR) as a sensitive assay of specific gene expression, we found that polyadenylated erbA alpha RNA is present in Xenopus cells at early developmental stages, including the fertilized egg, blastula, gastrula, and neurula. By performing erbA alpha-specific PCR on reverse-transcribed RNAs from high-density sucrose gradient fractions prepared from early-stage embryos, we have demonstrated that these erbA transcripts are recruited to polysomes. Therefore, erbA is expressed in Xenopus development prior to the appearance of the thyroid gland anlage in tailbud-stage embryos. This implies that erbA alpha/thyroid hormone receptors may play ligand-independent roles during the early development of X. laevis. Quantitative PCR revealed a greater than 25-fold range in the steady-state levels of polyadenylated erbA alpha RNA across early stages of development, as expressed relative to equimolar amounts of total embryonic RNA. Substantial increases in the levels of erbA alpha RNA were noted at stages well after the onset of zygotic transcription at the mid-blastula transition, with accumulation of erbA alpha transcripts reaching a relative maximum in advance of metamorphosis. We also show that erbA alpha RNAs are expressed unequally across Xenopus neural tube embryos. This differential expression continues through later stages of development, including metamorphosis. This finding suggests that erbA alpha/thyroid hormone receptors may play roles in tissue-specific processes across all of Xenopus development.


2009 ◽  
Vol 37 (2) ◽  
pp. 79-87 ◽  
Author(s):  
Keiko Motoyama ◽  
Yuji Nakai ◽  
Tomoya Miyashita ◽  
Yuichiro Fukui ◽  
Maki Morita ◽  
...  

To elucidate the physiological responses to a social stressor, we exposed mice to an isolation stress and analyzed their hepatic gene expression profiles using a DNA microarray. Male BALB/c mice were exposed to isolation stress for 30 days, and then hepatic RNA was sampled and subjected to DNA microarray analysis. The isolation stress altered the expression of 420 genes (after considering the false discovery rate). Gene Ontology analysis of these differentially expressed genes indicated that the stress remarkably downregulated the lipid metabolism-related pathway through peroxisome proliferator-activated receptor-α, while the lipid biosynthesis pathway controlled by sterol regulatory element binding factor 1, Golgi vesicle transport, and secretory pathway-related genes were significantly upregulated. These results suggest that isolation for 30 days with a mild and consecutive social stress regulates the systems for lipid metabolism and also causes endoplasmic reticulum stress in mouse liver.


Endocrinology ◽  
2010 ◽  
Vol 151 (6) ◽  
pp. 2946-2956 ◽  
Author(s):  
Madesh Belakavadi ◽  
Jason Saunders ◽  
Noah Weisleder ◽  
Preethi S. Raghava ◽  
Joseph D. Fondell

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