scholarly journals Oxalic acid and sclerotial differentiation of Polyporus umbellatus

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Yong-Mei Xing ◽  
Wan-Qiang Yin ◽  
Meng-Meng Liu ◽  
Chun-Lan Wang ◽  
Shun-Xing Guo

2020 ◽  
Author(s):  
Yong-Mei Xing ◽  
Bing Li ◽  
Xu Zeng ◽  
Li-Si Zhou ◽  
Tae-Soo Lee ◽  
...  

Abstract Polyporus umbellatus is a precious medicinal fungus. The whole transcriptome of P. umbellatus exposed to different concentrations of oxalic acid was performed and analyzed using RNA-seq based on sequencing technology. Reactive oxygen species (ROS) detection of P. umbellatus mycelia was visually conducted and using non-invasive micro-test technology (NMT), the net Ca2+ and H2O2 fluxes of P. umbellatus were measured. Totally, 22,523 unigenes were generated by De novo assembly of reads and there are 1223 differentially expressed genes (DEGs) between the control group and the high oxalic acid complemented (PD_S) group and 459 DEGs between the control group and the low concentration oxalic acid additive (PU_SM) group. The transcriptomic analysis indicated DEGs encoding enzymes related to oxidative stress, energy metabolism and so on. Compared to that of the control group, the biomass of the sclerotia in the PU_SM group increased 66%, however, no sclerotia formed in the PD_S group. The low concentration of oxalic acid could increase Ca2+ and H2O2 influx, while the high concentration of oxalic acid presented slight H2O2 efflux. There is a great significant positive correlation between the net Ca2+ and H2O2 fluxes. Different concentrations of exogenous oxalic acid affected P. umbellatus sclerotial formation in different ways.



2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yong-Mei Xing ◽  
Bing Li ◽  
Xu Zeng ◽  
Li-Si Zhou ◽  
Tae-Soo Lee ◽  
...  

AbstractPolyporus umbellatus is a precious medicinal fungus. Oxalic acid was observed to affect sclerotial formation and sclerotia possessed more medicinal compounds than mycelia. In this study, the transcriptome of P. umbellatus was analysed after the fungus was exposed to various concentrations of oxalic acid. The differentially expressed genes (DEGs) encoding a series of oxidases were upregulated, and reductases were downregulated, in the low-oxalic-acid (Low OA) group compared to the control (No OA) group, while the opposite phenomenon was observed in the high-oxalic-acid (High OA) group. The detection of reactive oxygen species (ROS) in P. umbellatus mycelia was performed visually, and Ca2+ and H2O2 fluxes were measured using non-invasive micro-test technology (NMT). The sclerotial biomass in the Low OA group increased by 66%, however, no sclerotia formed in the High OA group. The ROS fluorescence intensity increased significantly in the Low OA group but decreased considerably in the High OA group. Ca2+ and H2O2 influx significantly increased in the Low OA group, while H2O2 exhibited efflux in the High OA group. A higher level of oxidative stress formed in the Low OA group. Different concentrations of oxalic acid were determined to affect P. umbellatus sclerotial formation in different ways.



2012 ◽  
Vol 11 (4) ◽  
pp. 957-960 ◽  
Author(s):  
W.-Q. Yin ◽  
S.-X. Guo ◽  
Y.-M. Xing ◽  
X.-K. Xing


Author(s):  
N.C. Lyon ◽  
W. C. Mueller

Schumacher and Halbsguth first demonstrated ectodesmata as pores or channels in the epidermal cell walls in haustoria of Cuscuta odorata L. by light microscopy in tissues fixed in a sublimate fixative (30% ethyl alcohol, 30 ml:glacial acetic acid, 10 ml: 65% nitric acid, 1 ml: 40% formaldehyde, 5 ml: oxalic acid, 2 g: mecuric chloride to saturation 2-3 g). Other workers have published electron micrographs of structures transversing the outer epidermal cell in thin sections of plant leaves that have been interpreted as ectodesmata. Such structures are evident following treatment with Hg++ or Ag+ salts and are only rarely observed by electron microscopy. If ectodesmata exist without such treatment, and are not artefacts, they would afford natural pathways of entry for applied foliar solutions and plant viruses.



TAPPI Journal ◽  
2011 ◽  
Vol 10 (5) ◽  
pp. 21-28 ◽  
Author(s):  
CARL HOUTMAN ◽  
ERIC HORN

Pilot data indicate that wood chip pretreatment with oxalic acid reduced the specific energy required to make thermomechanical pulp. A combined oxalic acid/bisulfite treatment resulted in 21% refiner energy savings and 13% increase in brightness for aspen. A low level of oxalic acid treatment was effective for spruce. Energy savings of 30% was observed with no significant change in strength properties. Adding bisulfite did not significantly increase the brightness of the spruce pulp. For pine, the optimum treatment was a moderate level of oxalic acid, which resulted in 34% energy savings and an increase in strength properties. For all of these treatments 1–3 w/w % carbohydrates were recovered, which can be fermented to produce ethanol. The extract sugar solution contained significant quantities of arabinose.







2020 ◽  
Author(s):  
Madhur Kumar Dubey ◽  
Naman Jain ◽  
Atul Kumar ◽  
Gaurang Deep ◽  
Md Sharib


1983 ◽  
Vol 48 (11) ◽  
pp. 3223-3228 ◽  
Author(s):  
Peter Ševčík ◽  
Ľubica Adamčíková

The kinetic parameters of the reaction steps of the oscillation cycle and the parameters of modified oscillation reactions of the Belousov-Zhabotinskii (BZ) type with oxalic acid, tartaric acid, and hypophosphite ions were compared with predictions of Edelson's analysis based on the mechanism of the classical BZ reaction.



1976 ◽  
Vol 85 (5) ◽  
pp. 617 ◽  
Author(s):  
D. G. OREOPOULOS
Keyword(s):  


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