scholarly journals Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Christian Hacker ◽  
Jalal Asadi ◽  
Christos Pliotas ◽  
Sophie Ferguson ◽  
Lee Sherry ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
New Approach ◽  
Transmission Electron ◽  
Nanoparticle Suspensions

scholarly journals Fine structural detection of calcium ions by photoconversion

2016 ◽  
Author(s):  
V. Poletto ◽  
V. Galimberti ◽  
G. Guerra ◽  
V. Rosti ◽  
F. Moccia ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Calcium Ions ◽  
High Spatial Resolution ◽  
New Approach ◽  
Photo Oxidation ◽  
Transmission Electron ◽  
Direct Irradiation ◽  
Calcium Detection

We propose a tool for a rapid high-resolution detection of calcium ions which can be used in parallel with other techniques. We have applied a new approach by  photo-oxidation of diaminobenzidine in presence of the emission of an excited fluorochrome specific for calcium detection. This method combines the selectivity of available fluorophores to the high spatial resolution offered by transmission electron microscopy to detect even fluorescing molecules even when present in low amounts in membrane-bounded organelles. We show in this paper that Mag-Fura 2 photoconversion via diaminobenzidine oxidation is an efficient way for localizing Ca2+ ions at EM level, is easily carried out and reproducible, and can be obtained on a good amount of cells, since the exposition in our conditions is not limited to the direct irradiation of the sample via an objective but obtained with a germicide lamp. The end product is sufficiently electron dense to be detected clearly when present in sufficient amount within a membrane boundary.

A Novel Approach of Core-Shell Ag@Pt Nanoparticles Production

2017 ◽  
Vol 886 ◽  
pp. 48-52
Author(s):  
Silvia Chowdhury ◽  
Faridah Yusof ◽  
Nadzril Sulaiman ◽  
Mohammad Omer Faruck
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Gas Sensors ◽  
Pt Nanoparticles ◽  
Core Shell ◽  
New Approach ◽  
Novel Approach ◽  
Transmission Electron ◽  
Car Exhaust ◽  
Vis Spectroscopy

Pt-based nanoparticles (NPs) have numerous applications, such as, as catalyst, in car exhaust systems, gas sensors, biosensors and cancer therapy. One of the Pt based NPs which has been successfully produced is core-shell Ag@Pt NPs. Numerous methods for the synthesis of this material have been reported. This paper reports a fully new approach of chemical mediated synthesis for core-shell Ag@Pt NPs. Characterization process for the synthesized Ag@Pt NPs, carried out by the UV-vis Spectroscopy, Transmission Electron Microscopy (TEM), High Resolution Transmission Electron Microscopy (HRTEM) showed that the core AgNPs have approximate sizes of 18 nm in diameter are shelled with Pt and the sizes of core-shell Ag@Pt NPs were estimated to be around 29 nm in diameter.

scholarly journals Mild Heating of Amphotericin B-Desoxycholate: Effects on Ultrastructure, In Vitro Activity and Toxicity, and Therapeutic Efficacy in Severe Candidiasis in Leukopenic Mice

2000 ◽  
Vol 44 (6) ◽  
pp. 1598-1603 ◽  
Author(s):  
Els W. M. van Etten ◽  
Wim van Vianen ◽  
Patty Roovers ◽  
Peter Frederik
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Amphotericin B ◽  
Therapeutic Efficacy ◽  
Fungal Infections ◽  
Laser Diffraction ◽  
In Vitro Activity ◽  
New Approach ◽  
Transmission Electron

ABSTRACT Heated (20 min at 70°C) amphotericin B-desoxycholate (hAMB-DOC) was further characterized, as was another formulation obtained after centrifugation (60 min, 3000 × g), hcAMB-DOC. Conventional AMB-DOC consisted of individual micelles (approximately 4 nm in diameter) and threadlike aggregated micelles, as revealed by cryo-transmission electron microscopy. For both hAMB-DOC and hcAMB-DOC, pleiomorphic cobweb structures were observed with a mean particle size of approximately 300 nm as determined by laser diffraction. The potent antifungal activity of AMB-DOC against Candida albicans is not reduced by heating. Effective killing of C. albicans(>99.9% within 6 h) was obtained at 0.1 mg/liter with each of the AMB formulations. For AMB-DOC, hAMB-DOC, and hcAMB-DOC, cation release (86Rb+) from C. albicans of ≥50% was observed at 0.8, 0.4, and 0.4 mg/liter, respectively. After heating of AMB-DOC, toxicity was reduced 16-fold as determined by red blood cell (RBC) lysis. For AMB-DOC, hAMB-DOC, and hcAMB-DOC, hemolysis of ≥50% was observed at 6.4, 102.4, and 102.4 mg/liter, respectively. In contrast, AMB-DOC and its derivates showed similar toxicities in terms of cation release from RBC. For AMB-DOC, hAMB-DOC, and hcAMB-DOC, cation release (86Rb+) of ≥50% was observed at 1.6, 0.8, and 0.8 mg/liter, respectively. In persistently leukopenic mice with severe invasive candidiasis, higher dosages of both hAMB-DOC and hcAMB-DOC were tolerated than those of conventional AMB-DOC (3 versus 0.8 mg/kg of body weight, respectively), resulting in significantly improved therapeutic efficacy. In conclusion, this new approach of heating AMB-DOC may be of great value for further optimizing the treatment of severe fungal infections.

New Technique for Successful Thermal Barrier Coating Specimen Preparation for Transmission Electron Microscopy

2000 ◽  
Vol 6 (3) ◽  
pp. 231-236
Author(s):  
M.R. Brickey ◽  
J.L. Lee
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Thermally Grown Oxide ◽  
Thermal Barrier ◽  
Specimen Preparation ◽  
Ion Milling ◽  
New Approach ◽  
Barrier Coating ◽  
Transmission Electron ◽  
Thermal Histories

Abstract Reliability of thermal barrier coatings (TBC) hinges on the adhesion of a thermally grown oxide scale to an insulative ceramic topcoat and an underlying metallic bondcoat. The width of the scale and its interfaces makes transmission electron microscopy (TEM) an appropriate tool for its analysis. However, specimen preparation has proven to be a challenging obstacle leading to a dearth of TEM research on TBCs. A new approach to cross-section TBC TEM specimen preparation is described. The principal advantages of this technique are reproducibility, reduced specimen damage, and time savings resulting from decreased ion milling. This technique has been successfully applied to numerous TBC specimens with various thermal histories.

A New Approach to Assess Gold Nanoparticle Uptake by Mammalian Cells: Combining Optical Dark-Field and Transmission Electron Microscopy

Small ◽  
2012 ◽  
Vol 8 (23) ◽  
pp. 3683-3690 ◽  
Author(s):  
Christina Rosman ◽  
Sebastien Pierrat ◽  
Andreas Henkel ◽  
Marco Tarantola ◽  
David Schneider ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Gold Nanoparticle ◽  
Mammalian Cells ◽  
Dark Field ◽  
Nanoparticle Uptake ◽  
New Approach ◽  
Transmission Electron

A Method for Preparation of Site-Specific Multiple Samples of Semiconductor Material for Transmission Electron Microscopy

2001 ◽  
Vol 7 (S2) ◽  
pp. 948-949
Author(s):  
R. S. Rai ◽  
S. Bagchi ◽  
L. Duncan ◽  
L. Prabhu ◽  
J. Beck ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Sample Preparation ◽  
Ion Beam ◽  
Preparation Technique ◽  
Cross Sectional ◽  
Site Specific ◽  
New Approach ◽  
Area Of Interest ◽  
Transmission Electron

In recent years, the availability of focused ion beam (FIB) milling systems has given a much-needed boost for transmission electron microscopy (TEM) as a technique for site-specific analysis. Much progress has been made in the area of site-specific cross-sectional and planar TEM sample preparation techniques. However, a continuing need exists to reduce the sample preparation time, in order to improve TEM cycle time for better support of process development, yield improvement and production in a high-volume industrial environment. Thus, a faster TEM sample preparation technique is always desirable to meet this demand. A new approach to TEM sample preparation is described in this paper.Following the new approach developed in the present work, one can prepare on a single TEM grid at least two different cross-sectional samples of site-specific device structures or up to four different cross-sectional samples of blanket films. Two different samples, each containing an area of interest near the center, are cleaved or cut to a width of about 1.25 mm; these samples may be from two separate locations of a wafer, or from two different wafers where TEM analyses are required.

Environmentally Friendly Synthesis of Hydroxyapatite Nanoplates and their Adsorption Behavior of Proteins

2010 ◽  
Vol 113-116 ◽  
pp. 1036-1039
Author(s):  
Li Ying Li ◽  
Wen Hua Song ◽  
Jie Lian ◽  
Jin Hua Zhang
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Environmentally Friendly ◽  
Adsorption Behavior ◽  
X Ray Diffraction ◽  
New Approach ◽  
X Ray ◽  
Transmission Electron ◽  
Hydroxyapatite Nanocrystals ◽  
Environmentally Friendly Method

By a environmentally friendly method with the control of anionic amino acid surfactant N-lauroylsarcosine sodium (Sar-Na), hydroxyapatite (HAP) nanoplates were synthesized and the adsorption behavior of bovine serum albumin (BSA) were carefully studied. The products were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Results showed that hydroxyapatite nanocrystals with lengths of 100 nm, a width of 10 nm and a high aspect ratio (10:1) were obtained. Results of UV-Vis spectrum indicate that prepared hydroxyapatite nanoplates can be effectively adsorbed BSA. The current work provides a new approach to produce biomaterials.

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