scholarly journals Electrochemical Sensing of Idarubicin—DNA Interaction Using Electropolymerized Azure B and Methylene Blue Mediation

Chemosensors ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 33
Author(s):  
Anastasia Goida ◽  
Yurii Kuzin ◽  
Vladimir Evtugyn ◽  
Anna Porfireva ◽  
Gennady Evtugyn ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Biological Fluids ◽  
Dna Interaction ◽  
Electrochemical Sensing ◽  
Dna Sensor ◽  
Azure B ◽  
Fish Sperm ◽  
Highly Sensitive

A highly sensitive electrochemical DNA sensor for detection of the chemotherapeutic drug idarubicin mediated by Methylene blue (MB) has been developed. DNA from fish sperm has been immobilized at the electropolymerized layers of Azure B. The incorporation of MB into the DNA layers substantially increased the sensor sensitivity. The concentration range for idarubicin determination by cyclic voltammetry was from 1 fM to 0.1 nM, with a limit of detection (LOD) of 0.3 fM. Electrochemical impedance spectroscopy (EIS) in the presence of a redox probe ([Fe(CN)6]3−/4−) allowed for the widening of a linear range of idarubicin detection from 1 fM to 100 nM, retaining LOD 0.3 fM. The DNA sensor has been tested in various real and artificial biological fluids with good recovery ranging between 90–110%. The sensor has been successfully used for impedimetric idarubicin detection in medical preparation Zavedos®. The developed DNA biosensor could be useful for the control of the level of idarubicin during cancer therapy as well as for pharmacokinetics studies.

scholarly journals Electrochemical DNA Sensor Based on the Copolymer of Proflavine and Azure B for Doxorubicin Determination

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 924
Author(s):  
Anna Porfireva ◽  
Gennady Evtugyn
Keyword(s):  
Polymer Film ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Polymer Surface ◽  
Charge Transfer Resistance ◽  
Dna Sensor ◽  
Transfer Resistance ◽  
Redox Probe ◽  
Azure B

A DNA sensor has been developed for the determination of doxorubicin by consecutive electropolymerization of an equimolar mixture of Azure B and proflavine and adsorption of native DNA from salmon sperm on a polymer film. Electrochemical investigation showed a difference in the behavior of individual drugs polymerized and their mixture. The use of the copolymer offered some advantages, i.e., a higher roughness of the surface, a wider range of the pH sensitivity of the response, a denser and more robust film, etc. The formation of the polymer film and its redox properties were studied using scanning electron microscopy and electrochemical impedance spectroscopy. For the doxorubicin determination, its solution was mixed with DNA and applied on the polymer surface. After that, charge transfer resistance was assessed in the presence of [Fe(CN)6]3−/4− as the redox probe. Its value regularly grew with the doxorubicin concentration in the range from 0.03 to 10 nM (limit of detection 0.01 nM). The DNA sensor was tested on the doxorubicin preparations and spiked samples mimicking blood serum. The recovery was found to be 98–106%. The DNA sensor developed can find application for the determination of drug residues in blood and for the pharmacokinetics studies.

scholarly journals Surface-Enhanced Oxidation and Determination of Isothipendyl Hydrochloride at an Electrochemical Sensing Film Constructed by Multiwalled Carbon Nanotubes

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
S. N. Prashanth ◽  
Shankara S. Kalanur ◽  
Nagappa L. Teradal ◽  
J. Seetharamappa
Keyword(s):  
Limit Of Detection ◽  
Biological Fluids ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
Good Precision ◽  
Multiwalled Carbon ◽  
Limit Of Quantification ◽  
Surface Enhanced ◽  
Ph Range

The electrochemical behavior of isothipendyl hydrochloride (IPH) was investigated at bare and multiwalled-carbon-nanotube modified glassy carbon electrode (MWCNT-GCE). IPH (55 μM) showed two oxidation peaks in Britton-Robinson (BR) buffer of pH 7.0. The oxidation process of IPH was observed to be irreversible over the pH range of 2.5–9.0. The influence of pH, scan rate, and concentration of the drug on anodic peak was studied. A differential pulse voltammetric method with good precision and accuracy was developed for the determination of IPH in pure and biological fluids. The peak current was found to be linearly dependent on the concentration of IPH in the range of 1.25–55 μM. The values of limit of detection and limit of quantification were noticed to be 0.284 and 0.949 μM, respectively.

scholarly journals Highly sensitive dual mode electrochemical platform for microRNA detection

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Pawan Jolly ◽  
Marina R. Batistuti ◽  
Anna Miodek ◽  
Pavel Zhurauski ◽  
Marcelo Mulato ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Dual Mode ◽  
Electrode Surfaces ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Detection Mode ◽  
Amplification Strategy

Abstract MicroRNAs (miRNAs) play crucial regulatory roles in various human diseases including cancer, making them promising biomarkers. However, given the low levels of miRNAs present in blood, their use as cancer biomarkers requires the development of simple and effective analytical methods. Herein, we report the development of a highly sensitive dual mode electrochemical platform for the detection of microRNAs. The platform was developed using peptide nucleic acids as probes on gold electrode surfaces to capture target miRNAs. A simple amplification strategy using gold nanoparticles has been employed exploiting the inherent charges of the nucleic acids. Electrochemical impedance spectroscopy was used to monitor the changes in capacitance upon any binding event, without the need for any redox markers. By using thiolated ferrocene, a complementary detection mode on the same sensor was developed where the increasing peaks of ferrocene were recorded using square wave voltammetry with increasing miRNA concentration. This dual-mode approach allows detection of miRNA with a limit of detection of 0.37 fM and a wide dynamic range from 1 fM to 100 nM along with clear distinction from mismatched target miRNA sequences. The electrochemical platform developed can be easily expanded to other miRNA/DNA detection along with the development of microarray platforms.

scholarly journals Abnormal Anionic Porphyrin Sensing Effect for HER2 Gene Related DNA Detection via Impedance Difference between MWCNTs and Single-Stranded DNA or Double-Stranded DNA

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2688 ◽  
Author(s):  
Jingheng Ning ◽  
Long Liu ◽  
Xin Luo ◽  
Min Wang ◽  
Donglin Liu ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Electrochemical Sensing ◽  
Her2 Gene ◽  
Single Stranded Dna ◽  
Double Stranded Dna ◽  
Efficient Detection ◽  
Target Dna ◽  
Cnts Surface

Human epidermal growth factor receptor 2 (HER2) is a key tumor marker for several common and deadly cancers. It is of great importance to develop efficient detection methods for its over-expression. In this work, an electrochemical impedance spectroscopy (EIS) method adjustable by anionic porphyrin for HER2 gene detection has been proposed, based on the impedance difference between multi-walled carbon nanotubes (MWCNTs) and DNA. The interesting finding herein is that with the addition of anionic porphyrin, i.e., meso-tetra(4-sulfophenyl)-porphyrin (TSPP), the impedance value obtained at a glass carbon electrode (GCE) modified with MWCNTs and a single stranded DNA (ssDNA), the probe DNA that might be assembled tightly onto MWCNTs through π-π stacking interaction, gets a slight decrease; however, the impedance value from a GCE modified with MWCNTs and a double stranded DNA (dsDNA), the hybrid of the probe DNA with a target DNA, which might be assembled loosely onto MWCNTs for the screening effect of phosphate backbones in dsDNA, gets an obvious decrease. The reason may be that on the one hand, being rich in negative sulfonate groups, TSPP will try to push DNA far away from CNTs surface due to its strong electrostatic repulsion towards DNA; on the other hand, rich in planar phenyl or pyrrole rings, TSPP will compete with DNA for the surface of CNTs since it can also be assembled onto CNTs through conjugative interactions. In this way, the “loosely assembled” dsDNA will be repelled by this anionic porphyrin and released off CNTs surface much more than the “tightly assembled” ssDNA, leading to a bigger difference in the impedance value between dsDNA and ssDNA. Thus, through the amplification effect of TSPP on the impedance difference, the perfectly matched target DNA could be easily determined by EIS without any label. Under the optimized experimental conditions, this electrochemical sensor shows an excellent linear response to target DNA in a concentration range of 2.0 × 10−11–2.0 × 10−6 M with a limit of detection (LOD) of 6.34 × 10−11 M (S/N = 3). This abnormally sensitive electrochemical sensing performance resulting from anionic porphyrin for DNA sequences specific to HER2 gene will offer considerable promise for tumor diagnosis and treatment.

scholarly journals Ecofriendly Long Life Nanocomposite Sensors for Determination of Carbachol in Presence of Choline: Application in Ophthalmic Solutions and Biological Fluids

Sensors ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 2357 ◽  
Author(s):  
Eman A. Al-Harbi ◽  
Mona H. Abdelrahman ◽  
Amira M. El-Kosasy
Keyword(s):  
Limit Of Detection ◽  
Biological Fluids ◽  
Ion Association ◽  
Life Time ◽  
Electrochemical Sensing ◽  
Great Promise ◽  
Official Method ◽  
Significant Difference ◽  
Long Life ◽  
Ophthalmic Solutions

Several emerging nano scale forms of carbon are showing great promise in electrochemical sensing such as graphene and multi-walled carbon nanotubes (MWCNTs). Herein we present an ecofriendly method to fabricate long life and sensitive ion selective sensors based on graphene and MWCNTs nanocomposites with no need for volatile organic solvents. Both sensors were fabricated, for the analysis of carbachol in ophthalmic solutions, plasma and urine where ion- association complex was formed between cationic carbachol and anionic Sodium tetra phenyl borate (NaTBP) in a ratio 1:1. Both sensors were evaluated according to the IUPAC recommendation data, revealing linear response in the concentration range 10−7 M to 10−2 M with near Nernstian slopes 50.80 ± 5 and 58.14 ± 3 mV/decade and correlation coefficients 0.9992 and 0.9998 for graphene and MWCNTs based sensors, respectively. Both sensors were successfully applied as stability indicating method for the analysis of carbachol in presence of its metabolite choline, in ophthalmic preparations, in plasma and urine showing good recovery percentage values. MWCNTs based sensor showed some advantages over graphene sensor regarding lower limit of detection (LOD), longer life time and higher selectivity towards carbachol. Statistical comparison of the proposed sensors with the official method showed no significant difference for accuracy and precision.

scholarly journals Differential Pulse Voltammetric Electrochemical Sensor for the Detection of Etidronic Acid in Pharmaceutical Samples by Using rGO-Ag@SiO2/Au PCB

Nanomaterials ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1368 ◽  
Author(s):  
Sathish Panneer Selvam ◽  
Somasekhar R. Chinnadayyala ◽  
Sungbo Cho ◽  
Kyusik Yun
Keyword(s):  
Electrochemical Sensor ◽  
Active Sites ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
Etidronic Acid ◽  
Active Surface Area ◽  
Transfer Resistance ◽  
Pharmaceutical Samples

An rGO-Ag@SiO2 nanocomposite-based electrochemical sensor was developed to detect etidronic acid (EA) using the differential pulse voltammetric (DPV) technique. Rapid self-assembly of the rGO-Ag@SiO2 nanocomposite was accomplished through probe sonication. The developed rGO-Ag@SiO2 nanocomposite was used as an electrochemical sensing platform by drop-casting on a gold (Au) printed circuit board (PCB). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) confirmed the enhanced electrochemical active surface area (ECASA) and low charge transfer resistance (Rct) of the rGO-Ag@SiO2/Au PCB. The accelerated electron transfer and the high number of active sites on the rGO-Ag@SiO2/Au PCB resulted in the electrochemical detection of EA through the DPV technique with a limit of detection (LOD) of 0.68 μM and a linear range of 2.0–200.0 μM. The constructed DPV sensor exhibited high selectivity toward EA, high reproducibility in terms of different Au PCBs, excellent repeatability, and long-term stability in storage at room temperature (25 °C). The real-time application of the rGO-Ag@SiO2/Au PCB for EA detection was investigated using EA-based pharmaceutical samples. Recovery percentages between 96.2% and 102.9% were obtained. The developed DPV sensor based on an rGO-Ag@SiO2/Au PCB could be used to detect other electrochemically active species following optimization under certain conditions.

scholarly journals An Innovative Nanobody-Based High-Biocompatibility Gold Interdigitated Microelectrode Electrochemical Bioimpedance Sensor for the Ultrasensitive Detection of Difenacoum in Human Serum

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 3930
Author(s):  
Liuchuan Guo ◽  
Sihan Wang ◽  
Zhiwei He ◽  
Jing Zhang ◽  
Xiaoli Zhu ◽  
...  
Keyword(s):  
Human Serum ◽  
Detection Method ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Intentional Ingestion ◽  
Accuracy And Precision ◽  
Highly Sensitive ◽  
Interdigitated Microelectrode ◽  
Polycrystalline Gold

Difenacoum (DIF) is one of the most widely used anticoagulant rodenticides. However, accidental or intentional ingestion of DIF seriously threatens humans and other non-target species. Therefore, a rapid and sensitive detection method to quantify DIF is urgently needed. In this study, one anti-DIF nanobody (Nb) was assembled on the surface of a gold interdigitated microelectrode (IDME) using an Au–S bond to fabricate a bioimpedance sensor. To improve the immobilization amount of Nbs on the electrode, a polycrystalline gold IDME was prepared to provide a larger surface and better biocompatibility. Thus, a novel and ultrasensitive bioimpedance sensor based on electrochemical impedance spectroscopy (EIS) was designed for the determination of DIF, and it displayed good reproducibility and stability in human serum. The proposed bioimpedance sensor displayed a wide working range, between 0.1–1000 pg/mL, with a limit of detection (LOD) of 0.1 pg/mL of DIF. This method exhibited excellent performance, good sensitivity, and reproducibility and achieved the highest sensitivity of all currently existing methods used to quantify DIF. The highly sensitive DIF detection of this proposed bioimpedance sensor indicates its potential as an efficacious approach for DIF monitoring in human serum with high accuracy and precision.

scholarly journals Voltammetric Amitriptyline Determination Using a Metal-Free Electrode Based on Phosphorus-Doped Multi-Walled Carbon Nanotubes

2022 ◽  
Author(s):  
Chanakarn Sanguarnsak ◽  
Kiattisak Promsuwan ◽  
Jenjira Saichanapan ◽  
Asamee Soleh ◽  
Kasrin Saisahas ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Electrochemical Sensing ◽  
Urine Samples ◽  
Electrochemical Characteristics ◽  
Sensing Element ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes ◽  
Phosphorus Doped

Abstract A new electrode material of phosphorus-doped multi-walled carbon nanotubes (P-MWCNTs) was developed as an electrochemical sensing element for amitriptyline (AMT). P-MWCNTs were hydrothermally synthesized and drop casted on a glassy carbon electrode (P-MWCNTs/GCE). The P-MWCNTs were morphologically, chemically and structurally characterized. The electrochemical characteristics of the P-MWCNTs/GCE were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and adsorptive stripping voltammetry (AdSV). The P-MWCNTs increased electron transfer at the GCE and the electrochemical conductivity of the electrode. Electrocatalytic activity toward the oxidation of AMT was excellent. In the optimal voltammetric condition, the P-MWCNTs/GCE produced linear ranges of 0.50 to 10 µg mL-1 and 10 to 40 µg mL-1. The limit of detection (LOD) and limit of quantification (LOQ) were 0.15 µg mL-1 (0.54 µM) and 0.52 µg mL-1 (1.80 µM), respectively. The developed sensor displayed good repeatability, reproducibility and specificity. The sensor successfully quantified AMT in pharmaceutical tablets, giving results consistent with spectrophotometric analysis. The sensor achieved recoveries from 98±2% to 101±5% from spiked urine samples. The proposed sensor could be applied to determine AMT in pharmaceutical and urine samples for forensic toxicology.

scholarly journals Label free, electric field mediated ultrasensitive electrochemical point-of-care device for CEA detection

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
B. Chakraborty ◽  
A. Das ◽  
N. Mandal ◽  
N. Samanta ◽  
N. Das ◽  
...  
Keyword(s):  
Electric Field ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Zno Nanorods ◽  
Hybrid Nanostructures ◽  
Electrochemical Sensing ◽  
Label Free ◽  
Electron Transfer Rate

AbstractDeveloping point-of-care (PoC) diagnostic platforms for carcinoembryonic antigen detection is essential. However, thefew implementations of transferring the signal amplification strategies in electrochemical sensing on paper-based platforms are not satisfactory in terms of detection limit (LOD). In the quest for pushing down LOD, majority of the research has been targeted towards development of improved nanostructured substrates for entrapping more analyte molecules and augmenting the electron transfer rate to the working electrode. But, such approaches have reached saturation. This paper focuses on enhancing the mass transport of the analyte towards the sensor surface through the application of an electric field, in graphene-ZnO nanorods heterostructure. These hybrid nanostructures have been deposited on flexible polyethylene terephthalate substrates with screen printed electrodes for PoC application. The ZnO nanorods have been functionalized with aptamers and the working sensor has been integrated with smartphone interfaced indigenously developed low cost potentiostat. The performance of the system, requiring only 50 µl analyte has been evaluated using electrochemical impedance spectroscopy and validated against commercially available ELISA kit. Limit of detection of 1 fg/ml in human serum with 6.5% coefficient of variation has been demonstrated, which is more than three orders of magnitude lower than the existing attempts on PoC device.

scholarly journals A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of PSA as a Specific Diagnostic Marker of Prostate Cancer

2020 ◽  
Vol 23 ◽  
pp. 243-258 ◽  
Author(s):  
Shokoufeh Hassani ◽  
Armin Salek Maghsoudi ◽  
Milad Rezaei Akmal ◽  
Soheila Rahmani Rahmani ◽  
Pouria Sarihi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Specific Antigen ◽  
Differential Pulse ◽  
Label Free ◽  
Serum Samples ◽  
Highly Sensitive ◽  
Set Up

Purpose: The current project aimed to design a simple, highly sensitive, and economical label-free electrochemical aptasensor for determination of prostate-specific antigen (PSA), as the gold standard biomarker for prostate cancer diagnosis. The aptasensor was set up using a screen-printed carbon electrode (SPCE) modified by gold nanoparticles (Au NPs) conjugated to thiolated aptamers. Methods: Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were implemented for electrochemical (EC) characterization of the aptasensor. The determination of PSA was also performed through differential pulse voltammetry (DPV) in [Fe (CN) 6]3-/4- electrolyte solution. Results: The present aptasensor was shown an outstanding linear response in the concentration range of 1 pg/mL - 200 ng/mL with a remarkably lower limit of detection of 0.077 pg/mL. The optimum concentration for PSA separation and the optimum incubation time for antigen-aptamer binding were determined by observing and electing the highest electrochemical responses in a specified time or concentration. Conclusion: According to the results of the specificity tests, the designed aptasensor did not show any significant interactions with other analytes in real samples. Clinical functionality of the aptasensor was appraised in serum samples of healthy individuals and patients examining the PSA level through the fabricated aptasensor and the reference methods. Both methods are comparable in sensitivity. The present fabricated PSA aptasensor with substantial characteristics of ultra-sensitivity and cost-effectiveness can be conventionally built and used for the routine check-up of the men for prostate problems.

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