Detection and Quantification of E. coli and Coliform Bacteria in Water Samples with a New Method Based on Fluorescence In Situ Hybridisation

2012 ◽  
pp. 123-130
Author(s):  
Michael Hügler ◽  
Karin Böckle ◽  
Ingrid Eberhagen ◽  
Karin Thelen ◽  
Claudia Beimfohr ◽  
...  
Keyword(s):  
Water Samples ◽  
New Method ◽  
Coliform Bacteria ◽  
E Coli ◽  
Detection And Quantification

CONSUMPTION OF RAW WATER – THE HEALTH RISKS RELATED TO THE PRESENCE OF HEAVY METALS AND „ESCHERICHIA COLI“

2021 ◽  
Author(s):  
Mirjana Grujović ◽  
◽  
Katarina Mladenović ◽  
Zoran Simić ◽  
Simona Đuretanović
Keyword(s):  
Escherichia Coli ◽  
Trace Elements ◽  
Health Risks ◽  
Water Samples ◽  
Major And Trace Elements ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Mountain Stream ◽  
E Coli ◽  
Colorimetric Test

This research aimed to investigate the quality of drinking water from the rural area of village Pajsijević (Šumadija, central Serbia). The water is consumed as raw since it is not purified or chlorinated before consumption. The water was collected at three sampling points – in the spring of Kotlenik Mountain stream (W1 sample), in the local reservoir (W2 sample), and from the tap (W3 sample). Also, the sediment samples (soil and sand) were analyzed, too. The health risks related to the presence and concentration of some major and trace elements (Ca, Cr, Cu, Fe, Mg, Mn, Ni, Zn) and N, NO, NN3, NH4, P, P2O5, and PO4 were evaluated. Additionally, the presence and the number of total coliform bacteria and Escherichia coli (as an indicator of fecal contamination) were evaluated. The concentrations of analyzed major and trace elements in all water samples were below those at which toxic effects may occur. The exception was the concentrations of Fe (2.02 – 2012 mg/L), which were higher than is allowed. The origin of Fe in water is from sediment (soil and sand), which also showed high content of Fe (3006.0 mg/g and 2229.9 mg/g, respectively). The results of the Colorimetric test indicated the presence of coliform bacteria as well as the presence of E. coli in all water samples. Further research needs to include characterization of isolated coliform bacteria and serological investigation of E. coli strains in order to evaluate the risks of consumption related to waterborne illness.

scholarly journals DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Jurnal BIOMA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Rezki Rachmawati ◽  
Muzajjanah Muzajjanah ◽  
Yoswita Rustam
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Coliform Bacterium ◽  
Probable Number ◽  
E Coli ◽  
Biological Contamination ◽  
Drinking Water Samples

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

scholarly journals Identifikasi Bakteri Escherichia coli dalam Air Minum Galon pada Kantin yang ada di Universitas Andalas Padang

2021 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Muhammad Rayhan Braja Gitawama ◽  
Netti Suharti ◽  
Nora Harminarti
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Data Analysis ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Drug Agency ◽  
Drinking Water Samples

The National Food and Drug Agency has controlled the refill drinking water in drinking water depots and also controlled food at school canteens ranging from elementary, junior high, to high school, but the National Food and Drug Agency never do food quality controlling in canteens at universities. Objectives: To identified the contamination by coliform and E. coli bacteria in drinking water at Andalas University canteen. Methods: This research was descriptive to identify coliform bacteria on  15 drinking water at the Andalas University faculty canteens.  Samples were taken directly using a sterile bottle, while data analysis using Most Probable Number  (MPN)  tables 5-1-1 and the presence of E. coli bacteria colonies from drinking water samples. Results: 9 of 15 water samples were contaminated by coliform bacteria with the highest MPN index of 240/100 ml that was found in 2 samples. From 9 samples containing coliform bacteria, all of them were found to contain E. coli bacteria. Conclusion: Most of the samples were contaminated by coliform and E. coli bacteria. Drinking water served using a kettle was more contaminated than drinking water served using gallons.Keywords: Coliform, Escherichia coli, MPN

scholarly journals Development of Fluorescence In Situ Hybridization as a Rapid, Accurate Method for Detecting Coliforms in Water Samples

Biosensors ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 8
Author(s):  
Jong-Tar Kuo ◽  
Li-Li Chang ◽  
Chia-Yuan Yen ◽  
Teh-Hua Tsai ◽  
Yu-Chi Chang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Water Samples ◽  
Domestic Wastewater ◽  
Detection Methods ◽  
Coliform Bacteria ◽  
Individual Species ◽  
Biosensor System ◽  
Wastewater Samples

Coliform bacteria are indicators of water quality; however, most detection methods for coliform bacteria are time-consuming and nonspecific. Here, we developed a fluorescence in situ hybridization (FISH) approach to detect four types of coliform bacteria, including Escherichia coli, Klebsiella pneumoniae, Enterobacter aerogenes, and Citrobacter freundii, simultaneously in water samples using specific probes for 16S rRNA. This FISH method was applied to detect coliform bacteria in simulated water and domestic wastewater samples and compared with traditional detection methods (e.g., plate counting, multiple-tube fermentation (MTF) technique, and membrane filter (MF) technique). Optimal FISH conditions for detecting the four types of coliforms were found to be fixation in 3% paraformaldehyde at 4 °C for 2 h and hybridization at 50 °C for 1.5 h. By comparing FISH with plate counting, MTF, MF, and a commercial detection kit, we found that FISH had the shortest detection time and highest accuracy for the identification of coliform bacteria in simulated water and domestic wastewater samples. Moreover, the developed method could simultaneously detect individual species and concentrations of coliform bacteria. Overall, our findings indicated that FISH could be used as a rapid, accurate biosensor system for simultaneously detecting four types of coliform bacteria to ensure water safety.

Impact of chlorine injury on reaction kinetics of coliforms and E. coli in colisureTM and LTB

1997 ◽  
Vol 35 (11-12) ◽  
pp. 419-422
Author(s):  
G. McFeters ◽  
M. Pickett ◽  
S. Broadaway ◽  
B. Pyle
Keyword(s):  
Surface Water ◽  
Water Samples ◽  
Natural Populations ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Total Coliforms ◽  
E Coli ◽  
Lag Period ◽  
Surface Water Samples ◽  
Over Time

This study compared a chromogenic and fluorogenic substrate based medium, Colisure, with the conventional fermentation based medium, lauryl tryptose broth (LTB), for the recovery of chlorine-injured and non-injured coliforms and E. coli. Mixed natural populations of coliforms from sewage, with and without chlorine exposure, and untreated surface water samples were examined following a USEPA established procedure. Colisure and LTB were examined after 20, 24, 28 and 48h incubation and reactions verified. There were slight increases in positive reactions over time with unchlorinated samples, for which the two media gave similar kinetic patterns. The increases over time with chlorinated samples were much greater than with unchlorinated samples. LTB gave significantly lower total positive responses and had a more pronounced lag period than did Colisure. Total coliform bacteria from surface water samples gave somewhat different reactions with more of an increase with time. Colisure recovered comparable or higher numbers of total coliforms and E. coli in 20–28h than LTB after 48h. These results confirm that Colisure gives improved recovery of chlorine-injured total coliforms and E. coli under conditions simulating treated drinking and source water, resulting in a more realistic estimate of the actual population of indicator bacteria in public water supplies.

Faecal contamination of public water sources in informal settlements of Kisumu City, western Kenya

2012 ◽  
Vol 66 (12) ◽  
pp. 2674-2681 ◽  
Author(s):  
Selpha Opisa ◽  
Maurice R. Odiere ◽  
Walter G. Z. O. Jura ◽  
Diana M. S. Karanja ◽  
Pauline N. M. Mwinzi
Keyword(s):  
Water Samples ◽  
Membrane Filtration ◽  
Informal Settlements ◽  
Water Sources ◽  
Coliform Bacteria ◽  
Potential Contribution ◽  
Western Kenya ◽  
Faecal Contamination ◽  
E Coli ◽  
Kisumu City

High levels of environmental contamination, often associated with improper waste and excreta management, are widespread among informal settlements within urban areas in developing countries. We determined the level of faecal contamination in domestic water sources and evaluated the potential contribution of these water sources to intestinal helminthiases in seven informal settlements of Kisumu City, western Kenya. Membrane filtration technique was used for enumeration of total and faecal (Escherichia coli) coliform bacteria in water samples collected from dams, rivers, springs and wells. Out of the 80 water sources sampled, 76 (95%) were highly contaminated with E coli. All water samples from unprotected wells (26) and 92.6% of samples from protected wells (25) were positive for E. coli. The highest and lowest E. coli densities were observed in samples from dams (3,800 ± 1,807 coliforms per 100 ml) and boreholes (419 ± 223 coliforms per 100 ml), respectively (p = 0.0321). Distance from pit latrines was negatively associated with E. coli coliform density for wells (r = −0.34, n = 53, p = 0.0142). Untreated well-water may not be suitable for human consumption, and its continued use constitutes a major health risk for the inhabitants of these informal settlements.

Development and validation of a FISH-based method for the detection and quantification of E. coli and coliform bacteria in water samples

2011 ◽  
Vol 64 (7) ◽  
pp. 1435-1442 ◽  
Author(s):  
Michael Hügler ◽  
Karin Böckle ◽  
Ingrid Eberhagen ◽  
Karin Thelen ◽  
Claudia Beimfohr ◽  
...  
Keyword(s):  
Water Samples ◽  
Fluorescent Dyes ◽  
Direct Detection ◽  
Simultaneous Detection ◽  
Agar Plate ◽  
Coliform Bacteria ◽  
Bacterial Cells ◽  
Indicator Organisms ◽  
E Coli ◽  
Pure Cultures

Monitoring of microbiological contaminants in water supplies requires fast and sensitive methods for the specific detection of indicator organisms or pathogens. We developed a protocol for the simultaneous detection of E. coli and coliform bacteria based on the Fluorescence in situ Hybridization (FISH) technology. This protocol consists of two approaches. The first allows the direct detection of single E. coli and coliform bacterial cells on the filter membranes. The second approach includes incubation of the filter membranes on a nutrient agar plate and subsequent detection of the grown micro-colonies. Both approaches were validated using drinking water samples spiked with pure cultures and naturally contaminated water samples. The effects of heat, chlorine and UV disinfection were also investigated. The micro-colony approach yielded very good results for all samples and conditions tested, and thus can be thoroughly recommended for usage as an alternative method to detect E. coli and coliform bacteria in water samples. However, during this study, some limitations became visible for the single cell approach. The method cannot be applied for water samples which have been disinfected by UV irradiation. In addition, our results indicated that green fluorescent dyes are not suitable to be used with chlorine disinfected samples.

scholarly journals DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Jurnal BIOMA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Rezki Rachmawati ◽  
Muzajjanah Muzajjanah ◽  
Yoswita Rustam
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Coliform Bacterium ◽  
Probable Number ◽  
E Coli ◽  
Biological Contamination ◽  
Drinking Water Samples

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

scholarly journals Pollution Physique Et Bactériologique De L’eau Du Fleuve Dans La Basse Vallée De L’ouémé Pendant Les Périodes De Basses Et Hautes Eaux Au Bénin

2017 ◽  
Vol 13 (33) ◽  
pp. 167
Author(s):  
Adjagodo A. ◽  
Agassounon Djikpo Tchibozo M. ◽  
Kelomè N.C. ◽  
Vissin E.W. ◽  
Agbossou E.
Keyword(s):  
Water Samples ◽  
Membrane Filtration ◽  
High Water ◽  
Total Coliforms ◽  
E Coli ◽  
Sampling Campaign ◽  
High Water Period ◽  
Thermotolerant Coliforms

The objective of this study is to assess the water river quality of the Lower Valley of Ouémé in Benin. Two river water sampling campaigns were carried out during low and high water. At each sampling campaign, eleven (11) sampling sites were selected and at each site four samples were taken, making a total of 44 water samples. Hydrogen potential, dissolved oxygen, suspended matter and turbidity were measured in situ. Fecal contamination germs such as total coliforms, thermotolerant coliforms, Escherichia coli and fecal enterococci were counted by the membrane filtration technique. The results of the physical analyzes reveal that the pH measured in the water samples conformed to the WHO standard except turbidity. The results of the bacteriological analyzes in 100 mL of water sample of the river reveal average bacterial loads in periods of low and high waters which vary respectively from 2.57.10 2 ± 451 CFU and 3.35.102 ± 459 CFU for total coliforms; 1.42.102 ± 315 CFU and 1.55.102 ± 251 for thermotolerant coliforms; 0,79.102 ± 216 CFU and 1.55.102 ± 251 for E. coli. The Enterococci are absent in the waters during these two periods. The high burden of these germs counted in the high water period may be due runoff phenomenon and unhygienic behavior observable in the study area.

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