Influence of length on cytotoxicity of multi-walled carbon nanotubes against human acute monocytic leukemia cell line THP-1 in vitro and subcutaneous tissue of rats in vivo

2005 ◽  
Vol 1 (2) ◽  
pp. 176 ◽  
Author(s):  
Yoshinori Sato ◽  
Atsuro Yokoyama ◽  
Ken-ichiro Shibata ◽  
Yuki Akimoto ◽  
Shin-ichi Ogino ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Subcutaneous Tissue ◽  
Leukemia Cell ◽  
Leukemia Cell Line ◽  
Acute Monocytic Leukemia ◽  
Monocytic Leukemia ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

scholarly journals Biodegradable multi-walled carbon nanotubes trigger anti-tumoral effects

Nanoscale ◽  
2018 ◽  
Vol 10 (23) ◽  
pp. 11013-11020 ◽  
Author(s):  
E. González-Lavado ◽  
N. Iturrioz-Rodríguez ◽  
E. Padín-González ◽  
J. González ◽  
L. García-Hevia ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Malignant Melanoma ◽  
Solid Tumors ◽  
Mild Oxidation ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Mild oxidation treatments improve the in vitro and in vivo macrophage biodegradation of carbon nanotubes that trigger remarkable anti-tumoral effects in malignant melanoma solid tumors produced in mice.

Morphine and cancer progression: Hydrogen peroxide points to need for more research

2018 ◽  
Vol 7 (2) ◽  
pp. 93-96 ◽  
Author(s):  
Herbert Bosshart, MD
Keyword(s):  
Hydrogen Peroxide ◽  
Cancer Progression ◽  
Leukemia Cell ◽  
Leukemia Cell Line ◽  
Acute Monocytic Leukemia ◽  
H2o2 Production ◽  
Monocytic Leukemia ◽  
Promoting Effect ◽  
Intractable Cancer Pain

Background: Morphine is widely used in the management of intractable cancer pain. However, conflicting views exist on two suspected nonanalgesic properties of morphine: suppression of immune function and inhibition of cancer progression.Methods: In vitro measurement of the tumor growth-inhibiting signaling molecule, hydrogen peroxide (H2O2), released from the cultured acute monocytic leukemia cell line, THP-1, in the presence or absence of morphine.Results: Morphine at concentrations of 10−8 M significantly reduced H2O2 release from THP-1 cells.Conclusions: These results provide a proof of concept for morphine’s ability to inhibit H2O2 production and release in a leukemia cell system and point to a possible and as yet unrecognized tumor-promoting effect of morphine. More research is needed to systematically examine this suspected morphine-associated tumor-promoting effect.

scholarly journals CRISPR-Cas9 Editing of Human Histone Deubiquitinase Gene USP16 in Human Monocytic Leukemia Cell Line THP-1

2021 ◽  
Vol 9 ◽  
Author(s):  
Iveta Gažová ◽  
Lucas Lefevre ◽  
Stephen J. Bush ◽  
Rocio Rojo ◽  
David A. Hume ◽  
...  
Keyword(s):  
Cell Line ◽  
Leukemia Cell ◽  
Leukemic Cell ◽  
Leukemia Cell Line ◽  
Acute Monocytic Leukemia ◽  
Wild Type ◽  
Monocytic Leukemia ◽  
Damage Repair ◽  
Apparent Loss

USP16 is a histone deubiquitinase which facilitates G2/M transition during the cell cycle, regulates DNA damage repair and contributes to inducible gene expression. We mutated the USP16 gene in a high differentiation clone of the acute monocytic leukemia cell line THP-1 using the CRISPR-Cas9 system and generated four homozygous knockout clones. All were able to proliferate and to differentiate in response to phorbol ester (PMA) treatment. One line was highly proliferative prior to PMA treatment and shut down proliferation upon differentiation, like wild type. Three clones showed sustained expression of the progenitor cell marker MYB, indicating that differentiation had not completely blocked proliferation in these clones. Network analysis of transcriptomic differences among wild type, heterozygotes and homozygotes showed clusters of genes that were up- or down-regulated after differentiation in all cell lines. Prior to PMA treatment, the homozygous clones had lower levels than wild type of genes relating to metabolism and mitochondria, including SRPRB, encoding an interaction partner of USP16. There was also apparent loss of interferon signaling. In contrast, a number of genes were up-regulated in the homozygous cells compared to wild type at baseline, including other deubiquitinases (USP12, BAP1, and MYSM1). However, three homozygotes failed to fully induce USP3 during differentiation. Other network clusters showed effects prior to or after differentiation in the homozygous clones. Thus the removal of USP16 affected the transcriptome of the cells, although all these lines were able to survive, which suggests that the functions attributed to USP16 may be redundant. Our analysis indicates that the leukemic line can adapt to the extreme selection pressure applied by the loss of USP16, and the harsh conditions of the gene editing and selection protocol, through different compensatory pathways. Similar selection pressures occur during the evolution of a cancer in vivo, and our results can be seen as a case study in leukemic cell adaptation. USP16 has been considered a target for cancer chemotherapy, but our results suggest that treatment would select for escape mutants that are resistant to USP16 inhibitors.

scholarly journals PDLLA honeycomb-like scaffolds with a high loading of superhydrophilic graphene/multi-walled carbon nanotubes promote osteoblast in vitro functions and guided in vivo bone regeneration

2017 ◽  
Vol 73 ◽  
pp. 31-39 ◽  
Author(s):  
Edmundo Silva ◽  
Luana Marotta Reis de Vasconcellos ◽  
Bruno V.M. Rodrigues ◽  
Danilo Martins dos Santos ◽  
Sergio P. Campana-Filho ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Bone Regeneration ◽  
High Loading ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

scholarly journals Cellular responses induced by multi-walled carbon nanotubes: in vivo and in vitro studies on the medicinal leech macrophages

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Rossana Girardello ◽  
Nicolò Baranzini ◽  
Gianluca Tettamanti ◽  
Magda de Eguileor ◽  
Annalisa Grimaldi
Keyword(s):  
Carbon Nanotubes ◽  
Cellular Responses ◽  
Medicinal Leech ◽  
In Vitro Studies ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

scholarly journals Production, characterization and immobilization of Aspergillus versicolor L-asparaginase onto multi-walled carbon nanotubes

2020 ◽  
Vol 10 (4) ◽  
pp. 5733-5740
Keyword(s):  
Carbon Nanotubes ◽  
Production Condition ◽  
Physical Adsorption ◽  
Maximum Yield ◽  
Normal Fibroblast ◽  
Aspergillus Versicolor ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

The goal of this work is to study the optimum production of L-ASNase and the influence of oxidized multi-walled carbon nanotubes (ox-MWCNTs) on the biological activity of the isolated L-ASNase. Isolation and production of L-ASNase from different screened fungi in the presence of L-asparagine substrate were carried out. The isolated enzyme was identified by molecular18S rRNA analysis. The partial purified L-ASNase having 6.77 purification fold was immobilized onto ox-MWCNTs using physical adsorption technique with maximum yield about 54.4%. In vitro cytotoxicity of L-ASNase against normal fibroblast cell line (BHK-21) was examined relative to the immobilized one using SRB assay. Also, in vivo biological examination using liver and kidney dysfunction biomarkers in the treated mice (AST, ALT, LDH, lipase and α-amylase) was also investigated. Among the ten screened fungi, Aspergillus versicolor was the most potent one with maximum activity about 45.5 U/mL. The results showed that the most appropriate production condition was at asparagine concentration of about 0.04 M and pH 8. The immobilized L-ASNase retained a complete activity (100%) after 30 min at 45ᴼC of incubation relative to that in case of the free one. The toxicity of L-ASNase was significantly decreased up to 50 μg/mL after immobilization onto MWCNTs. Besides, the immobilized L-ASNase exhibited good storage ability and stability at high temperature relative to that in case of the free one.

Evaluation of toxicity of halloysite nanotubes and multi-walled carbon nanotubes to endothelial cells in vitro and blood vessels in vivo

2020 ◽  
Vol 14 (8) ◽  
pp. 1017-1038 ◽  
Author(s):  
Bihan Wu ◽  
Mengdie Jiang ◽  
Xuewu Liu ◽  
Chaobo Huang ◽  
Zhipeng Gu ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Endothelial Cells ◽  
Blood Vessels ◽  
Halloysite Nanotubes ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

The Effects of the Coating of Anodized Titanium with Multi-Walled Carbon Nanotubes on Bone Formation

2012 ◽  
Vol 529-530 ◽  
pp. 621-624
Author(s):  
Saori Inoue ◽  
Motohiro Uo ◽  
Masatoshi Sakairi ◽  
Eri Hirata ◽  
Min Ho Lee ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Bone Formation ◽  
Bone Tissue ◽  
Biological Properties ◽  
Adhesion Protein ◽  
Multi Walled Carbon Nanotubes ◽  
Anodized Titanium ◽  
Walled Carbon Nanotubes

Carbon nanotubes (CNTs) have excellent chemical, physical, and biological properties such as strong cell adhesion, protein adsorption and cell proliferationin vitro. Excellent osteocompatibility for the CNT monolith was also reported in vivo. The purpose of this study was to evaluate the effects of anodized titanium coated with multiwalled CNTs (MWCNTs) on human osteosarcoma Saos2 cells and bone tissue. Saos2 cells on CNT-Ti showed excellent proliferation with extension of cell morphology in all directions. CNT-Ti wire was implanted in the bone marrow of femurs of rats. At 2 weeks after surgery, histological investigations revealed that bone tissue attached to the surface of the CNT-Ti directly. Thus the surface modification of anodized Ti by MWCNTs can be effective for bone formation.

Sign in / Sign up

Export Citation Format

Share Document