Probing the influence of cis–trans isomers on model lipid membrane fluidity using cis-parinaric acid and a stop-flow technique

AbstractListeria monocytogenes is a food-borne pathogen with the ability to grow at low temperatures down to − 0.4 °C. Maintaining cytoplasmic membrane fluidity by changing the lipid membrane composition is important during growth at low temperatures. In Listeria monocytogenes, the dominant adaptation effect is the fluidization of the membrane by shortening of fatty acid chain length. In some strains, however, an additional response is the increase in menaquinone content during growth at low temperatures. The increase of this neutral lipid leads to fluidization of the membrane and thus represents a mechanism that is complementary to the fatty acid-mediated modification of membrane fluidity. This study demonstrated that the reduction of menaquinone content for Listeria monocytogenes strains resulted in significantly lower resistance to temperature stress and lower growth rates compared to unaffected control cultures after growth at 6 °C. Menaquinone content was reduced by supplementation with aromatic amino acids, which led to a feedback inhibition of the menaquinone synthesis. Menaquinone-reduced Listeria monocytogenes strains showed reduced bacterial cell fitness. This confirmed the adaptive function of menaquinones for growth at low temperatures of this pathogen.

Download Full-text

An increase in model lipid membrane fluidity as a result of local anesthetic action

Cell and Tissue Biology ◽

10.1134/s1990519x16040052 ◽

2016 ◽

Vol 10 (4) ◽

pp. 324-331 ◽

Cited By ~ 3

Author(s):

S. S. Efimova ◽

R. Ya. Medvedev ◽

L. V. Schagina ◽

O. S. Ostroumova

Keyword(s):

Membrane Fluidity ◽

Local Anesthetic ◽

Lipid Membrane ◽

Anesthetic Action

Download Full-text

Impact of selected polyphenolics on the structural properties of model lipid membranes – a review

International Journal of Food Studies ◽

10.7455/ijfs.v6i2.391 ◽

2017 ◽

Vol 6 (2) ◽

Cited By ~ 1

Author(s):

Nataša P. Ulrih ◽

Ajda Ota ◽

Veronika Abram

Keyword(s):

Electron Paramagnetic Resonance ◽

Membrane Fluidity ◽

Phenolic Acids ◽

Lipid Membranes ◽

Lipid Membrane ◽

Biological Effects ◽

Resonance Spectroscopy ◽

Paramagnetic Resonance ◽

Model Lipid Membranes ◽

Electron Paramagnetic

This review is a presentation of data gathered on the interactions of several polyphenolics (i.e., phenolic acids, stilbenes, flavonoids) with lipid bilayers of different lipid compositions. These polyphenolics have been investigated through a combination of fluorescence spectroscopy, electron paramagnetic resonance spectroscopy, and differential scanning calorimetry, to detect changes in membrane fluidity. Among the investigated phenolic acids, the least polar phenolic acid, p-coumaric acid, has the greatest effect on lipid membrane structure. It appears to have a greater ability to cross membranes by passive transport than more polar phenolic acids. On the other hand, among the flavonoids that have been studied, the anthocyanins cyanidin 3-glucoside and its aglycone are inactive. All of the flavonols tested, except for epigallocatechin-3-gallate, promote small decreases in membrane fluidity. Computer simulation of electron paramagnetic resonance spectra for flavonoids indicated two or three regions in the phosphatidylcholine/ phosphatidylserine (2.4:1) membrane with different fluidity characteristics. The effects of the different flavonoids are correlated to their structural characteristics, whereby even the difference in one -OH group can be important, as can the number of H-bonds they form. The role of membrane composition and flavonoid structure in these interactions with lipid membranes are of great importance for bioavailability of these compounds and for their biological effects in an organism

Download Full-text

Control of Lipid Self-Assembled Structures & Assessment of Lipid Membrane Fluidity by Fluorescence Spectroscopy

Journal of Oleo Science ◽

10.5650/jos.ess19244 ◽

2020 ◽

Vol 69 (2) ◽

pp. 83-91

Author(s):

Makoto Uyama

Keyword(s):

Fluorescence Spectroscopy ◽

Membrane Fluidity ◽

Lipid Membrane ◽

Self Assembled

Download Full-text

Impact of humic acids on EYL liposome membranes: ESR method

Nukleonika ◽

10.1515/nuka-2015-0081 ◽

2015 ◽

Vol 60 (3) ◽

pp. 455-459 ◽

Cited By ~ 1

Author(s):

Barbara Pytel ◽

Aneta Filipiak ◽

Izabella Pisarek ◽

Ryszard Olchawa ◽

Dariusz Man

Keyword(s):

Humic Substances ◽

Membrane Fluidity ◽

Lipid Bilayer ◽

Humic Acids ◽

Lipid Membrane ◽

Surface Region ◽

Egg Yolk ◽

Fulvic Acids ◽

Spin Resonance ◽

Mass Concentrations

AbstractIn this paper, the effects of model (commercial) and natural (extracted from peat) humic substances on the membrane of liposomes formed with egg yolk lecithin (EYL) are presented. In our research, mass concentrations of fulvic and humic acids were used, which in relation to lecithin varied from 0% to 13%. To study membrane fluidity, electron spin resonance (EPR) was used with two spin probes, penetrating various regions of the lipid bilayer. The effects of model and natural humic substances (humic acids – HAs and fulvic acids – FAs) on the lipid membrane in different regions were researched: the lipid-water interphase, and in the middle of the lipid bilayer. It was shown that FA and HA impact the fluidity of liposome membranes in different ways. Increased mass concentrations of HAs decreased membrane fluidity in both acids: extracted from peat and the model. However, increased mass concentration of FAs extracted from peat, decreased membrane fluidity in the surface region, at the same time stiffening the central part of the bilayer. Increasing the concentration of FAs extracted from peat had the opposite effect when compared to model FA. This effect may be related to the complexation of xenobiotics present in the soil environment and their impact on biological membranes.

Download Full-text

Effects of fatty acids on BK channels in GH3cells

AJP Cell Physiology ◽

10.1152/ajpcell.2000.279.4.c1211 ◽

2000 ◽

Vol 279 (4) ◽

pp. C1211-C1219 ◽

Cited By ~ 50

Author(s):

Donald D. Denson ◽

Xiaoping Wang ◽

Roger T. Worrell ◽

Douglas C. Eaton

Keyword(s):

Fatty Acids ◽

Membrane Fluidity ◽

Direct Interaction ◽

Bk Channels ◽

Bk Channel ◽

Channel Activity ◽

Trans Isomers ◽

Open Probability ◽

Cytosolic Phospholipase ◽

Excised Patches

Ca2+-activated K+ (BK) channels in GH3 cells are activated by arachidonic acid (AA). Because cytosolic phospholipase A2 can produce other unsaturated free fatty acids (FFA), we examined the effects of FFA on BK channels in excised patches. Control recordings were made at several holding potentials. The desired FFA was added to the bath solution, and the voltage paradigm was repeated. AA increased the activity of BK channels by 3.6 ± 1.6-fold. The cis FFA, palmitoleic, oleic, linoleic, linolenic, eicosapentaenoic, and the triple bond analog of AA, eicosatetraynoic acid, all increased BK channel activity, whereas stearic (saturated) or the trans isomers elaidic, linolelaidic, and linolenelaidic had no effect. The cisunsaturated FFA shifted the open probability vs. voltage relationships to the left without a change in slope, suggesting no change in the sensitivity of the voltage sensor. Measurements of membrane fluidity showed no correlation between the change of membrane fluidity and the change in BK channel activation. In addition, AA effects on BK channels were unaffected in the presence of N-acetylcysteine. Arachidonyl-CoA, a membrane impermeable analog of AA, activates channels when applied to the cytosolic surface of excised patches, suggesting an effect of FFAs from the cytosolic surface of BK channels. Our data imply a direct interaction between cis FFA and the BK channel protein.

Download Full-text