scholarly journals Spatially resolved shear distribution in microfluidic chip for studying force transduction mechanisms in cells

Lab on a Chip ◽  
2010 ◽  
Vol 10 (2) ◽  
pp. 235-239 ◽  
Author(s):  
Jianbin Wang ◽  
Jinseok Heo ◽  
Susan Z. Hua
Keyword(s):  
Microfluidic Chip ◽  
Spatially Resolved ◽  
Shear Distribution ◽  
Transduction Mechanisms ◽  
In Cells ◽  
Force Transduction

scholarly journals Metavinculin modulates force transduction in cell adhesion sites

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Verena Kanoldt ◽  
Carleen Kluger ◽  
Christiane Barz ◽  
Anna-Lena Schweizer ◽  
Deepak Ramanujam ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Heart Muscle ◽  
Tissue Response ◽  
Adhesion Forces ◽  
Muscle Disorders ◽  
Adhesion Sites ◽  
Molecular Forces ◽  
In Cells ◽  
Force Transduction

AbstractVinculin is a ubiquitously expressed protein, crucial for the regulation of force transduction in cells. Muscle cells express a vinculin splice-isoform called metavinculin, which has been associated with cardiomyopathies. However, the molecular function of metavinculin has remained unclear and its role for heart muscle disorders undefined. Here, we have employed a set of piconewton-sensitive tension sensors to probe metavinculin mechanics in cells. Our experiments reveal that metavinculin bears higher molecular forces but is less frequently engaged as compared to vinculin, leading to altered force propagation in cell adhesions. In addition, we have generated knockout mice to investigate the consequences of metavinculin loss in vivo. Unexpectedly, these animals display an unaltered tissue response in a cardiac hypertrophy model. Together, the data reveal that the transduction of cell adhesion forces is modulated by expression of metavinculin, yet its role for heart muscle function seems more subtle than previously thought.

Dealing with mechanics: mechanisms of force transduction in cells

2004 ◽  
Vol 29 (7) ◽  
pp. 364-370 ◽  
Author(s):  
Paul A. Janmey ◽  
David A. Weitz
Keyword(s):  
In Cells ◽  
Force Transduction

Analysis of Mechanisms of Cellular Injury on the Micrometer Scale

1997 ◽  
Vol 3 (S2) ◽  
pp. 37-38
Author(s):  
Robert C. Burghardt ◽  
Rola Barhoumi
Keyword(s):  
Fluorescent Probes ◽  
Molecular Mechanisms ◽  
Cultured Cells ◽  
Rapid Identification ◽  
Fluorescence Probes ◽  
Ros Generation ◽  
Mechanisms Of Toxicity ◽  
Transduction Mechanisms ◽  
Cellular Signal ◽  
In Cells

Non-invasive imaging tools using biosensors and biomarkers for defining the function of living cells and tissues are among rapidly emerging technologies being developed to monitor cellular toxicity mechanisms. Commercially available fluorescence probes and naturally occurring fluorescent molecules can be used to quantitatively monitor a number of functional endpoints in cultured cells. Innovative approaches to exploit the sensitivity, spectroscopy and temporal/spatial resolution properties of fluorescent probes may provide sensitive approaches for analysis of the molecular mechanisms of toxicity in cells exposed to a variety of toxicants. Kinetic analysis of multiple cellular parameters such as intracellular glutathione (GSH) and Ca2+ content, reactive oxygen species (ROS) generation, mitochondrial and plasma membrane potentials, intracellular pH, and gap junction-mediated intercellular communication (GJIC) with cellular component-specific fluorescent probes permits rapid identification of changes in these parameters and the chronology of injury in cells caused by acute toxicant exposure. Cellular signal transduction mechanisms are also likely to be vulnerable to toxic insults.

Three-dimensional Mach-Zehnder interferometer in a microfluidic chip for spatially-resolved label-free detection

Lab on a Chip ◽  
2010 ◽  
Vol 10 (9) ◽  
pp. 1167 ◽  
Author(s):  
Andrea Crespi ◽  
Yu Gu ◽  
Bongkot Ngamsom ◽  
Hugo J. W. M. Hoekstra ◽  
Chaitanya Dongre ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Three Dimensional ◽  
Zehnder Interferometer ◽  
Label Free ◽  
Spatially Resolved ◽  
Label Free Detection ◽  
Mach Zehnder Interferometer

An integrated microfluidic chip enabling control and spatially resolved monitoring of temperature in micro flow reactors

2014 ◽  
Vol 407 (2) ◽  
pp. 387-396 ◽  
Author(s):  
Christian Hoera ◽  
Stefan Ohla ◽  
Zhe Shu ◽  
Erik Beckert ◽  
Stefan Nagl ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Flow Reactors ◽  
Spatially Resolved ◽  
Micro Flow

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

Electron Channeling Patterns

1977 ◽  
Vol 35 ◽  
pp. 12-13
Author(s):  
David C. Joy
Keyword(s):  
Electron Diffraction ◽  
Incidence Angle ◽  
Photographic Plate ◽  
Diffraction Effect ◽  
Angle Of Incidence ◽  
Bulk Single Crystal ◽  
Time Resolved ◽  
Electron Channeling ◽  
Spatially Resolved ◽  
Large Bulk

Electron channeling patterns (ECP) were first found by Coates (1967) while observing a large bulk, single crystal of silicon in a scanning electron microscope. The geometric pattern visible was shown to be produced as a result of the changes in the angle of incidence, between the beam and the specimen surface normal, which occur when the sample is examined at low magnification (Booker, Shaw, Whelan and Hirsch 1967).A conventional electron diffraction pattern consists of an angularly resolved intensity distribution in space which may be directly viewed on a fluorescent screen or recorded on a photographic plate. An ECP, on the other hand, is produced as the result of changes in the signal collected by a suitable electron detector as the incidence angle is varied. If an integrating detector is used, or if the beam traverses the surface at a fixed angle, then no channeling contrast will be observed. The ECP is thus a time resolved electron diffraction effect. It can therefore be related to spatially resolved diffraction phenomena by an application of the concepts of reciprocity (Cowley 1969).

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