Biocompatible succinic acid-based polyesters for potential biomedical applications: fungal biofilm inhibition and mesenchymal stem cell growth

RSC Advances ◽  
2015 ◽  
Vol 5 (104) ◽  
pp. 85756-85766 ◽  
Author(s):  
E. Jäger ◽  
R. K. Donato ◽  
M. Perchacz ◽  
A. Jäger ◽  
F. Surman ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Cell Growth ◽  
Mesenchymal Stem Cell ◽  
Medical Devices ◽  
Biomedical Applications ◽  
Growth Promotion ◽  
Intrinsic Properties ◽  
Biofilm Inhibition

Poly(alkene succinates) are promising materials for specialized medical devices and tissue engineering, presenting intrinsic properties, such as; fungal biofilm inhibition, biocompatibility and stem cells controlled growth promotion.

scholarly journals Advances in Regenerative Stem Cell Therapy in Androgenic Alopecia and Hair Loss: Wnt Pathway, Growth-Factor, and Mesenchymal Stem Cell Signaling Impact Analysis on Cell Growth and Hair Follicle Development

Cells ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 466 ◽  
Author(s):  
Pietro Gentile ◽  
Simone Garcovich
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Growth ◽  
Mesenchymal Stem Cell ◽  
Hair Follicle ◽  
Hair Loss ◽  
Hair Growth ◽  
Follicle Development ◽  
Hair Regrowth ◽  
Hair Follicle Development

The use of stem cells has been reported to improve hair regrowth in several therapeutic strategies, including reversing the pathological mechanisms, that contribute to hair loss, regeneration of hair follicles, or creating hair using the tissue-engineering approach. Although various promising stem cell approaches are progressing via pre-clinical models to clinical trials, intraoperative stem cell treatments with a one-step procedure offer a quicker result by incorporating an autologous cell source without manipulation, which may be injected by surgeons through a well-established clinical practice. Many authors have concentrated on adipose-derived stromal vascular cells due to their ability to separate into numerous cell genealogies, platelet-rich plasma for its ability to enhance cell multiplication and neo-angiogenesis, as well as human follicle mesenchymal stem cells. In this paper, the significant improvements in intraoperative stem cell approaches, from in vivo models to clinical investigations, are reviewed. The potential regenerative instruments and functions of various cell populaces in the hair regrowth process are discussed. The addition of Wnt signaling in dermal papilla cells is considered a key factor in stimulating hair growth. Mesenchymal stem cell-derived signaling and growth factors obtained by platelets influence hair growth through cellular proliferation to prolong the anagen phase (FGF-7), induce cell growth (ERK activation), stimulate hair follicle development (β-catenin), and suppress apoptotic cues (Bcl-2 release and Akt activation).

scholarly journals Mesenchymal stem cell like (MSCl) cells generated from human embryonic stem cells support pluripotent cell growth

2011 ◽  
Vol 414 (3) ◽  
pp. 474-480 ◽  
Author(s):  
Nóra Varga ◽  
Zoltán Veréb ◽  
Éva Rajnavölgyi ◽  
Katalin Német ◽  
Ferenc Uher ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Cell Growth ◽  
Mesenchymal Stem Cell ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Pluripotent Cell

Two novel amino acid-coated super paramagnetic nanoparticles at low concentrations label and promote the proliferation of mesenchymal stem cells

RSC Advances ◽  
2016 ◽  
Vol 6 (12) ◽  
pp. 10159-10161 ◽  
Author(s):  
Zhe-Zhen Yu ◽  
Qing-Hua Wu ◽  
Shang-Li Zhang ◽  
Jun-Ying Miao ◽  
Bao- Xiang Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Amino Acid ◽  
Magnetic Nanoparticles ◽  
Cell Growth ◽  
Mesenchymal Stem Cell ◽  
S Phase ◽  
Low Concentrations

We identified two amino acid-coated magnetic nanoparticles that promoted mesenchymal stem cell growth without the need for transfection agents by increasing the proportion of cells in the S phase.

Branched Channel Scaffolds Fabricated by SFF for Direct Cell Growth Observations

2009 ◽  
Vol 24 (1_suppl) ◽  
pp. 63-74 ◽  
Author(s):  
Shanglong Xu ◽  
Yue Yang ◽  
Xibin Wang ◽  
Chaofeng Wang
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Cell Growth ◽  
Mesenchymal Stem Cell ◽  
Channel Structure ◽  
Free Form ◽  
Channel System ◽  
Direct Cell ◽  
Time Observation ◽  
Solid Free Form Fabrication

A β-TCP scaffold with a branched channel system was designed to create a novel micro-device that allowed culture perfusion and direct time observation of the cells attached. The scaffold was made by indirect solid free form fabrication (SFF) technology. The flow channel structure was exposed so that the perfusion of the mesenchymal stem cell (MSC) culture could be viewed directly. The cell-seeded scaffolds were continuously perfused for 7 days in the micro-device; during this time, it was possible to observe the dynamic culture processes with cells adhering to the scaffolds and real time cell growth directly. This concept has great potential for use in bone tissue engineering and for versatile fabrication of enhanced scaffolds.

Microenvironment of macula flava in the human vocal fold as a stem cell niche

2016 ◽  
Vol 130 (7) ◽  
pp. 656-661 ◽  
Author(s):  
K Sato ◽  
S Chitose ◽  
T Kurita ◽  
H Umeno
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Division ◽  
Cell Growth ◽  
Mesenchymal Stem Cell ◽  
Growth Medium ◽  
Stem Cell Niche ◽  
Vocal Fold ◽  
Colony Forming Unit ◽  
Cell Niche

AbstractBackground:There is growing evidence that the cells in the maculae flavae are tissue stem cells of the human vocal fold mucosa, and that the maculae flavae are a candidate for a stem cell niche. The role of microenvironment in the maculae flavae of the human vocal fold mucosa was investigated.Method:Anterior maculae flavae from six surgical specimens were cultured in a mesenchymal stem cell growth medium or a Dulbecco's modified Eagle's medium.Results:Using mesenchymal stem cell growth medium, the subcultured cells formed a colony-forming unit, and cell division reflected asymmetric self-renewal. This indicates that these cells are mesenchymal stem cells or stromal stem cells in the bone marrow. Using Dulbecco's modified Eagle's medium, the subcultured cells showed symmetric cell division without a colony-forming unit.Conclusion:A proper microenvironment in the maculae flavae of the human vocal fold mucosa is necessary to be effective as a stem cell niche that maintains the stemness of the contained tissue stem cells.

scholarly journals Engineered scaffolds based on mesenchymal stem cells/preosteoclasts extracellular matrix promote bone regeneration

2020 ◽  
Vol 11 ◽  
pp. 204173142092691 ◽  
Author(s):  
Rui Dong ◽  
Yun Bai ◽  
Jingjin Dai ◽  
Moyuan Deng ◽  
Chunrong Zhao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Complex Biological Process

Recently, extracellular matrix-based tissue-engineered bone is a promising approach to repairing bone defects, and the seed cells are mostly mesenchymal stem cells. However, bone remodelling is a complex biological process, in which osteoclasts perform bone resorption and osteoblasts dominate bone formation. The interaction and coupling of these two kinds of cells is the key to bone repair. Therefore, the extracellular matrix secreted by the mesenchymal stem cells alone cannot mimic a complex bone regeneration microenvironment, and the addition of extracellular matrix by preosteoclasts may contribute as an effective strategy for bone regeneration. Here, we established the mesenchymal stem cell/preosteoclast extracellular matrix -based tissue-engineered bones and demonstrated that engineered-scaffolds based on mesenchymal stem cell/ preosteoclast extracellular matrix significantly enhanced osteogenesis in a 3 mm rat femur defect model compared with mesenchymal stem cell alone. The bioactive proteins released from the mesenchymal stem cell/ preosteoclast extracellular matrix based tissue-engineered bones also promoted the migration, adhesion, and osteogenic differentiation of mesenchymal stem cells in vitro. As for the mechanisms, the iTRAQ-labeled mass spectrometry was performed, and 608 differentially expressed proteins were found, including the IGFBP5 and CXCL12. Through in vitro studies, we proved that CXCL12 and IGFBP5 proteins, mainly released from the preosteoclasts, contributed to mesenchymal stem cells migration and osteogenic differentiation, respectively. Overall, our research, for the first time, introduce pre-osteoclast into the tissue engineering of bone and optimize the strategy of constructing extracellular matrix–based tissue-engineered bone using different cells to simulate the natural bone regeneration environment, which provides new sight for bone tissue engineering.

scholarly journals Regenerative medicine in dental and oral tissues: Dental pulp mesenchymal stem cell

2016 ◽  
Vol 28 (1) ◽  
Author(s):  
Janti Sudiono ◽  
Ciptadhi Tri Oka ◽  
Melanie S Djamil ◽  
Ferry Sandra
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Mesenchymal Stem Cell ◽  
Dental Pulp ◽  
Periodontal Ligament ◽  
Therapeutic Modality ◽  
Regenerative Capacity

Background. Regenerative medicine is a new therapeutic modality using cell, stem cell and tissue engineering technologies. Purpose. To describe the regenerative capacity of dental pulp mesenchymal stem cell. Review. In dentistry, stem cell and tissue engineering technologies develop incredibly and attract great interest, due to the capacity to facilitate innovation in dental material and regeneration of dental and oral tissues. Mesenchymal stem cells derived from dental pulp, periodontal ligament and dental follicle, can be isolated, cultured and differentiated into various cells, so that can be useful for regeneration of dental, nerves, periodontal and bone tissues. Tissue engineering is a technology in reconstructive biology, which utilizes mechanical, cellular, or biological mediators to facilitate regeneration or reconstruction of a particular tissue. The multipotency, high proliferation rates and accessibility, make dental pulp as an attractive source of mesenchymal stem cells for tissue regeneration. Revitalized dental pulp and continued root development is the focus of regenerative endodontic while biological techniques that can restore lost alveolar bone, periodontal ligament, and root cementum is the focus of regenerative periodontic. Conclucion. Dentin-derived morphogens such as BMP are known to be involved in the regulation of odontogenesis. The multipotency and angiogenic capacity of DPSCs as the regenerative capacity of human dentin / pulp complex indicated that dental pulp may contain progenitors that are responsible for dentin repair. The human periodontal ligament is a viable alternative source for possible primitive precursors to be used in stem cell therapy.

scholarly journals Isolation of a multipotent mesenchymal stem cell-like population from human adrenal cortex

2018 ◽  
Vol 7 (5) ◽  
pp. 617-629 ◽  
Author(s):  
Earn H Gan ◽  
Wendy Robson ◽  
Peter Murphy ◽  
Robert Pickard ◽  
Simon Pearce ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Flow Cytometry ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Surface ◽  
Adrenal Cortex ◽  
Cell Biology ◽  
Growth Promotion ◽  
Pluripotency Markers

Background The highly plastic nature of adrenal cortex suggests the presence of adrenocortical stem cells (ACSC), but the exact in vivo identity of ACSC remains elusive. A few studies have demonstrated the differentiation of adipose or bone marrow-derived mesenchymal stem cells (MSC) into steroid-producing cells. We therefore investigated the isolation of multipotent MSC from human adrenal cortex. Methods Human adrenals were obtained as discarded surgical material. Single-cell suspensions from human adrenal cortex (n = 3) were cultured onto either complete growth medium (CM) or MSC growth promotion medium (MGPM) in hypoxic condition. Following ex vivo expansion, their multilineage differentiation capacity was evaluated. Phenotype markers were analysed by immunocytochemistry and flow cytometry for cell-surface antigens associated with bone marrow MSCs and adrenocortical-specific phenotype. Expression of mRNAs for pluripotency markers was assessed by q-PCR. Results The formation of colony-forming unit fibroblasts comprising adherent cells with fibroblast-like morphology were observed from the monolayer cell culture, in both CM and MGPM. Cells derived from MGPM revealed differentiation towards osteogenic and adipogenic cell lineages. These cells expressed cell-surface MSC markers (CD44, CD90, CD105 and CD166) but did not express the haematopoietic, lymphocytic or HLA-DR markers. Flow cytometry demonstrated significantly higher expression of GLI1 in cell population harvested from MGPM, which were highly proliferative. They also exhibited increased expression of the pluripotency markers. Conclusion Our study demonstrates that human adrenal cortex harbours a mesenchymal stem cell-like population. Understanding the cell biology of adrenal cortex- derived MSCs will inform regenerative medicine approaches in autoimmune Addison’s disease.

In vivo study of the angiogenesis potential of bone marrow-derived mesenchymal stem cell aggregates in their niche like environment

2021 ◽  
pp. 039139882110255
Author(s):  
Sara Anajafi ◽  
Azam Ranjbar ◽  
Monireh Torabi-Rahvar ◽  
Naser Ahmadbeigi
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cultured Cells ◽  
Morphological Evidence ◽  
Cell Aggregates ◽  
Plla Scaffold ◽  
Significant Difference

Background: Sufficient blood vessel formation in bioengineered tissues is essential in order to keep the viability of the organs. Impaired development of blood vasculatures results in failure of the implanted tissue. The cellular source which is seeded in the scaffold is one of the crucial factors involved in tissue engineering methods. Materials and methods: Considering the notable competence of Bone Marrow derived Mesenchymal Stem Cell aggregates for tissue engineering purposes, in this study BM-aggregates and expanded BM-MSCs were applied without any inductive agent or co-cultured cells, in order to investigate their own angiogenesis potency in vivo. BM-aggregates and BM-MSC were seeded in Poly-L Lactic acid (PLLA) scaffold and implanted in the peritoneal cavity of mice. Result: Immunohistochemistry results indicated that there was a significant difference ( p < 0.050) in CD31+ cells between PLLA scaffolds contained cultured BM-MSC; PLLA scaffolds contained BM-aggregates and empty PLLA. According to morphological evidence, obvious connections with recipient vasculature and acceptable integration with surroundings were established in MSC and aggregate-seeded scaffolds. Conclusion: Our findings revealed cultured BM-MSC and BM-aggregates, capacity in order to develop numerous connections between PLLA scaffold and recipient’s vasculature which is crucial to the survival of tissues, and considerable tendency to develop constructs containing CD31+ endothelial cells which can contribute in vessel’s tube formation.

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