Naked eye and optical biosensing of cysteine over the other amino acids using β-cyclodextrin decorated silver nanoparticles as a nanoprobe

2018 ◽  
Vol 42 (11) ◽  
pp. 9193-9199 ◽  
Author(s):  
Ramar Rajamanikandan ◽  
Malaichamy Ilanchelian

A simple, highly selective and efficient sensing approach based on a β-CD AgNP colorimetric nanoprobe has been demonstrated, which permits quick and specific determination of Cys over other important amino acids.

Author(s):  
George Joseph ◽  
Asha Varughese ◽  
Ann Daniel

Abstract Background A method has been developed and validated for selective, accurate and precise determination of total proteinogenic amino acids and taurine from Infant Formula and Adult/Pediatric Nutritional Formulas (powders, ready-to-feed liquids, and liquid concentrates). The method was reviewed by the AOAC INTERNATIONAL SPIFAN Expert Review Panel (ERP) during the 133rd AOAC Annual Meeting & Expo on September 7, 2019 in Denver, CO, USA and was recommended to First Action Official MethodsSM status. Objective The method involves protein hydrolysis to amino acids, a simple pre-column derivatisation of amino acids and separation of derivatised amino acids by UHPLC. The quantification of amino acids is performed by multi-point calibration using norvaline as the internal standard. The analytical method is capable of quantitative determination for 22 proteinogenic amino acids, but cannot be used to quantitate tryptophan, which is destroyed during the acid hydrolysis step. Asparagine is determined as aspartic acid and glutamine as glutamic acid. The cystine and cysteine are converted to S-2-carboxyethylthiocysteine (CYSx) and the derivative is separated from the other amino acids. Citrulline which is present in some matrices and it is separated from other amino acids is not included in the method performance evaluation in the single laboratory validation (SLV). Method The proposed method met all the performance requirement limits set in standard method performance requirements (SMPR) 2014.013 for total proteinogenic amino acids and taurine. The correlation coefficient of multi-point calibration was not less than 0.999 for any amino acids at any point in the SLV study confirming the validity of linear dynanic range (LDR) and linearity of the method. The individual amino acids in the chromatogram were identified by absolute retention time and relative retention time (RRT) with respect to the internal standard norvaline. There were no significant (S/N Ratio <10) interferences from the reagents or by-products of derivatisation and targeted matrices. The method demonstrated high selectivity. Result Accuracy of the method was validated using standard reference materials (NIST SRM 1869 and 1849a) and spike recovery studies. The amino acid results in the SRMs were within the ranges of Reference Mass Fraction Values. The accuracy of the method was corroboratively validated by spike recovery studies. The average spike recovery range between 93 to 107% ensure the accuracy of the method for amino acids and compliance to the AOAC SMPR 2014.013. Conclusions Precision data of the method demonstrate that it meets the stakeholder requirements as per the SMPR. The mean RSDr for all the amino acids for 17 matrices selected for the SLV were not more than 4%. The method is very sensitive and the LOQ can go down to approximately ten times lower than the SMPR requirements. The sensitivity of method is a direct reflection of its signal to noise ratio which ensures guaranteed method performance at the lower levels of amino acids in these matrices. Highlights Taurine (aminoethane sulfonic acid) unlike the other amino acids is a beta-sulfonic amino acid that is not used in protein synthesis but is found as a free amino acid in tissues. The acidic functional group (-COOH) in common amino acid is replaced with a sulfonic acid (-SO3H) group in Taurine. The method offers baseline separation of citrulline which is an alpha amino acid generally present in Infant Formula and Adult/Pediatric Nutritional products. The separation of citrulline eliminates the risk of interference of this compound with other amino acids. The method can also separate and quantitate hydroxyproline, an important component of collagen that is often used to quantitate collagen. The method is simple and does not include any proprietary chemicals or instruments and can be performed on any basic reverse phase UHPLC system with UV detection.


1961 ◽  
Vol 06 (03) ◽  
pp. 462-469 ◽  
Author(s):  
R Gross ◽  
W Gerok

SummaryIn normal human platelets twenty amino acids and additionally taurine, phosphoethanolamine and phosphoserine were determined on a quantitative basis, using elution chromatography on ion exchangers.The highest molar share goes to taurine, glutamic acid, alanine, aspartic acid, proline and serine. The other amino acids can be found in free form only in a low concentration in platelets.The connection of these results with other investigations on the metabolism is discussed.


2021 ◽  
pp. 130218
Author(s):  
Jinkun Huang ◽  
Xiaomei Mo ◽  
Haiyan Fu ◽  
Yao Sun ◽  
Qian Gao ◽  
...  

2017 ◽  
Vol 100 (6) ◽  
pp. 1814-1818 ◽  
Author(s):  
Stig Valdersnes ◽  
Anita Birkenes ◽  
Livar Froyland

Abstract The aim of this study was to develop and validate a method for the determination of balenine/ophidine (hereafter referred to as “balenine”) in whale extracts and muscle samples from Balaenoptera acutorostrata. Further, the goal was to evaluate the method’s applicability for the determination of other histidine-containing dipeptides (HCDs): anserine and carnosine and their amino acids π-methylhistidine, τ-methylhistidine, histidine, and β-alanine. For balenine, the LOD and LOQ were found to be 0.03 and 0.1 mg/g, respectively, and the linear range was validated up to 160 mg/g. Trueness was evaluated by spiking experiments with balenine, and the recovery was found to be 88–90%. A comparison of the results showed that most of the other analytes were within 80–120% of the value found with the previously developed and validated method. Precision and internal reproducibility for balenine was around 0.9 and 2%, respectively, with measurement uncertainties of 2–4%. Therefore, the method was found to be fit for purpose for the determination of balenine and other HCDs and their constituent amino acids in whale meat and extracts.


1968 ◽  
Vol 48 (3) ◽  
pp. 349-354 ◽  
Author(s):  
F. J. Sowden ◽  
K. C. Ivarson

A system of separating asparagine, glutamine and citrulline from each other and from the other common amino acids, using lithium buffers and the Technicon amino acid analyzer and C2 resin in a 0.63 × 75-cm column, is described. The column was operated with a buffer flow rate of 37 ml/hour at 30 °C for the first 70 min, then at 50 °C. The buffer was 0.067 M in lithium citrate and adjusted to a pH of 2.80; 2% isopropanol was added to improve the separation of threonine and serine. The analysis was complete through citrulline in 4 hours. If a second buffer, pH 4.15, 0.25 N in lithium was added after 3.5 hours, most of the common acidic and neutral amino acids found in soil extracts were separated in 6 hours. Some data on the determination of asparagine and glutamine by amide hydrolysis is included.


1917 ◽  
Vol 25 (2) ◽  
pp. 255-272 ◽  
Author(s):  
Mataro Nagayo ◽  
Yoneji Miyagawa ◽  
Tokushiro Mitamura ◽  
Arao Imamura

Nomenclature. As mentioned above, the prosopon and the nymph of the tsutsugamushi have many characteristics which distinguish it from the other trombidia. These are the pale color and small size of the body, delicacy of the skin, the conspicuous constriction between the cephalothoracic region and the abdomen, rudimentary eyes and tracheal openings, absence of tracheal capillaries, etc. The fact that the thumb or the appendage of the palpus does not start from the side of the fourth joint of the latter but from its end, is another charac teristic which does not occur in other trombidia. From the biological point of view two facts should be mentioned; i.e., the adult female does not deposit eggs in heaps, and in contrast to those of other species, the larvaæ hatch out all through the year and feed on mammals. From these characteristics running through all the stages of development, we believe that we are justified in claiming a new genus for the tsutsugamushi. We therefore propose to change the scientific name given to this mite by Brumpt in 1910, Trombidium akamushi, and to introduce instead, according to the suggestion of Drs. Goto and Watase, the name Leptotrombidium akamushi. Determinaton of Leptotrombidium akamushi, N. Sp. Prosopon and Nymph. 1. Pale color. 2. Delicacy of the skin. 3. Distinct constriction between the cephalothoracic region and the abdomen. 4. Rudimentary, sessile eyes. 5. Indistinctnes of tracheal openings. 6. Absence of tracheal capillaries. 7. Crista abruptly widened at the end. 8. One palpal claw. Larva. 1. One scutum. 2. Round pinacles. 3. Legs, six joints, not counting the coxa. 4. Haris on the coza: one each on Joints 1, 2, and 3. 5. Hairs on the scutum, including two tactile hairs, seven; one is in the median line. Specific Determination of Leptotrombidium akamushi, N. Sp. (Trombidium akamushi Brumpt, 1910). Prosopon. 1. Small size 2. Three pairs of sucking discs on the external genitalia. 3. Hairs of the body feathered, club-shaped at the end, and the colorless. 4. The appendage of the palpus tapes distinctly towards the end. Nymph. 1. The size of the body is smaller thant that of the prosopon. 2. Two pairs of sucking discs on the external genitalia. There are no other distinct differences in the external morphology between the proposon and the nymph. Larva. 1. The color of the body is orange-red, but becomes paler after sucking on mammals. 2. The hairs of the body average 110 to 120 in number. 3. One pair of double eyes. 4. The sucking tube is visible when feeding on mammals (Hayashi). 5. The salivary gland is relatively large (Kawamura and Komagata). 6. The number and arrangemetn of the hairs on the legs and mouth parts correspond to the description given by Hirst. 7. The larvæ are found all through the year. 8. The larva is the carrier of tsutsugamushi disease in man.


Author(s):  
D.R. Rasmussen ◽  
N.-H. Cho ◽  
C.B. Carter

Domains in GaAs can exist which are related to one another by the inversion symmetry, i.e., the sites of gallium and arsenic in one domain are interchanged in the other domain. The boundary between these two different domains is known as an antiphase boundary [1], In the terminology used to describe grain boundaries, the grains on either side of this boundary can be regarded as being Σ=1-related. For the {110} interface plane, in particular, there are equal numbers of GaGa and As-As anti-site bonds across the interface. The equilibrium distance between two atoms of the same kind crossing the boundary is expected to be different from the length of normal GaAs bonds in the bulk. Therefore, the relative position of each grain on either side of an APB may be translated such that the boundary can have a lower energy situation. This translation does not affect the perfect Σ=1 coincidence site relationship. Such a lattice translation is expected for all high-angle grain boundaries as a way of relaxation of the boundary structure.


Author(s):  
Y. Ishida ◽  
H. Ishida ◽  
K. Kohra ◽  
H. Ichinose

IntroductionA simple and accurate technique to determine the Burgers vector of a dislocation has become feasible with the advent of HVEM. The conventional image vanishing technique(1) using Bragg conditions with the diffraction vector perpendicular to the Burgers vector suffers from various drawbacks; The dislocation image appears even when the g.b = 0 criterion is satisfied, if the edge component of the dislocation is large. On the other hand, the image disappears for certain high order diffractions even when g.b ≠ 0. Furthermore, the determination of the magnitude of the Burgers vector is not easy with the criterion. Recent image simulation technique is free from the ambiguities but require too many parameters for the computation. The weak-beam “fringe counting” technique investigated in the present study is immune from the problems. Even the magnitude of the Burgers vector is determined from the number of the terminating thickness fringes at the exit of the dislocation in wedge shaped foil surfaces.


1962 ◽  
Vol 08 (03) ◽  
pp. 434-441 ◽  
Author(s):  
Edmond R Cole ◽  
Ewa Marciniak ◽  
Walter H Seegers

SummaryTwo quantitative procedures for autoprothrombin C are described. In one of these purified prothrombin is used as a substrate, and the activity of autoprothrombin C can be measured even if thrombin is in the preparation. In this procedure a reaction mixture is used wherein the thrombin titer which develops in 20 minutes is proportional to the autoprothrombin C in the reaction mixture. A unit is defined as the amount which will generate 70 units of thrombin in the standardized reaction mixture. In the other method thrombin interferes with the result, because a standard bovine plasma sample is recalcified and the clotting time is noted. Autoprothrombin C shortens the clotting time, and the extent of this is a quantitative measure of autoprothrombin C activity.


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