Fluorometric detection of EGFR exon 19 deletion mutation in lung cancer cells using graphene oxide

Abstract Non–small cell lung cancer with pituitary metastasis (NSCLC-PM) is a devastating disease; however, treatment is being revolutionized by a novel therapy targeting highly specific tumor signals, such as the mutation of epidermal growth factor receptors (EGFRs). Long-term management of hormonal defects in this population has become a unique neuroendocrine clinical challenge. We report the case of a 73-year-old female nonsmoker who was diagnosed with stage IV non–small cell lung cancer. The initial staging evaluation revealed a 7 × 11 × 21-mm sellar lesion abutting the optic chiasm and causing clinical hypopituitarism. The patient received three cycles of chemotherapy with carboplatin and pemetrexed, which was discontinued because of major cumulative side effects of myelosuppression and kidney disease. Eight months later, scans demonstrated evidence of disease progression. A repeated lung nodule biopsy revealed an EGFR exon 19 deletion mutation. EGFR-targeted therapy with osimertinib 80 mg daily was initiated. A complete resolution of the pituitary lesion was evident on a follow-up pituitary MRI 5 weeks later and was sustained 1 year after. However, the panhypopituitarism persisted. This is an illustrative case of NSCLC-PM with EGFR exon 19 deletion mutation, wherein osimertinib, a third-generation EGFR‒tyrosine kinase inhibitor, eradicated the sellar metastasis and prevented the need for radiotherapy. However, the neuroendocrine deficits persisted despite anatomic improvement.

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In vitrotoxic effects of reduced graphene oxide nanosheets on lung cancer cells

Nanotechnology ◽

10.1088/1361-6528/aa95a8 ◽

2017 ◽

Vol 28 (50) ◽

pp. 504001 ◽

Cited By ~ 29

Author(s):

Tanveer A Tabish ◽

Md Zahidul I Pranjol ◽

Hasan Hayat ◽

Alma A M Rahat ◽

Trefa M Abdullah ◽

...

Keyword(s):

Lung Cancer ◽

Graphene Oxide ◽

Cancer Cells ◽

Reduced Graphene Oxide ◽

Lung Cancer Cells ◽

Graphene Oxide Nanosheets ◽

Reduced Graphene

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Dose-dependent genotoxicity of ammonia-modified graphene oxide particles in lung cancer cells

Journal of Physics Conference Series ◽

10.1088/1742-6596/1186/1/012009 ◽

2019 ◽

Vol 1186 ◽

pp. 012009

Author(s):

K Hristova-Panusheva ◽

M Keremidarska-Markova ◽

T Andreeva ◽

G Speranza ◽

D Wang ◽

...

Keyword(s):

Lung Cancer ◽

Graphene Oxide ◽

Cancer Cells ◽

Lung Cancer Cells ◽

Modified Graphene Oxide ◽

Oxide Particles ◽

Modified Graphene ◽

Dose Dependent

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Cytotoxicity Evaluation of Ammonia-Modified Graphene Oxide Particles in Lung Cancer Cells and Embryonic Stem Cells

Advances in Condensed Matter Physics ◽

10.1155/2018/9571828 ◽

2018 ◽

Vol 2018 ◽

pp. 1-11 ◽

Cited By ~ 3

Author(s):

Milena Keremidarska-Markova ◽

Kamelia Hristova-Panusheva ◽

Tonya Andreeva ◽

Giorgio Speranza ◽

Dayong Wang ◽

...

Keyword(s):

Lung Cancer ◽

Stem Cells ◽

Graphene Oxide ◽

Embryonic Stem Cells ◽

Cancer Cells ◽

Human Lung ◽

Embryonic Stem ◽

A549 Cells ◽

Lung Cancer Cells ◽

Human Lung Cancer

Potential toxicity of graphene oxide (GO) is a subject of increasing research interest in the recent years. Here, we have evaluated the cytotoxicity of ammonia-modified GO (GO-NH2) and pristine GO particles in human lung cancer cells, A549 and embryonic stem cells, Lep3 exposed to different particles concentrations (0.1, 1, 10, 20, and 50 μg/ml) for different times (24 and 48h). Compared with GO, GO-NH2 particles possessed smaller size, positive surface charge and higher thickness. An increased propensity to aggregation in cell cultures was also found for GO-NH2 particles. Cytotoxicity evaluation revealed that GO-NH2 particles are more toxic than pristine GO. Applied at concentrations of 10, 20 and 50 μg/ml for 24h they affect significantly cell morphology of viable embryonic stem cells whereas human lung cancer A549 cells seem to be relatively more resistant to short-time exposure. After 48h exposure however cell proliferation of A549 cells was strongly suppressed in a dose-dependent manner while the proliferation ability of embryonic stem cells was not affected. These results suggested that both GO particles exert different degree of cytotoxicity which is time, dose and cell dependent. In general, ammonia-modified GO particles are more toxic than the pristine GO which should be taken into account for future biomedical applications.

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