scholarly journals Towards identifying the mode of action of drugs using live-cell FTIR spectroscopy

The Analyst ◽  
2019 ◽  
Vol 144 (8) ◽  
pp. 2725-2735 ◽  
Author(s):  
Ali Altharawi ◽  
Khondaker Miraz Rahman ◽  
K. L. Andrew Chan
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ftir Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Mode Of Action ◽  
Fourier Transform Infrared ◽  
Live Cell ◽  
Promising Tool

Fourier transform infrared spectroscopy (FTIR) has been shown to be a promising tool for identifying the mode of action of drugs.

Fourier-transform infrared spectroscopy for rapid screening and live-cell monitoring: application to nanotoxicology

Nanomedicine ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. 145-156 ◽  
Author(s):  
SK Sundaram ◽  
Colette A Sacksteder ◽  
Thomas J Weber ◽  
Brian J Riley ◽  
R Shane Addleman ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Live Cell ◽  
Rapid Screening ◽  
Cell Monitoring ◽  
Monitoring Application

Time-resolved Fourier-transform infrared spectroscopy reveals the hidden bimolecular process of the ferrioxalate actinometer

2019 ◽  
Vol 21 (43) ◽  
pp. 23803-23807 ◽  
Author(s):  
Frank Hendrik Pilz ◽  
Jörg Lindner ◽  
Peter Vöhringer
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ftir Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Molecular Level ◽  
Fourier Transform Infrared ◽  
Bimolecular Reaction ◽  
Time Resolved

Step-scan FTIR-spectroscopy reveals the bimolecular reaction in the ferrioxalate photochemistry, which builds the molecular-level foundation of the Hatchard–Parker actinometer.

scholarly journals Detection of Animal Fat Mixtures in Meatballs Using Fourier Transform Infrared Spectroscopy (FTIR Spectroscopy)

2021 ◽  
Vol 3 (1) ◽  
pp. 8-12
Author(s):  
Imelda Fajriati ◽  
Yusi Rosadi ◽  
Nisrina Nabila Rosadi ◽  
Khamidinal Khamidinal
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ftir Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Soxhlet Extraction ◽  
Fourier Transform Infrared ◽  
Extraction Temperature ◽  
Chicken Fat ◽  
Animal Fats ◽  
Animal Fat

Detection of animal mixtures in meatballs by using Fourier Transform Infrared Spectroscopy (FTIR spectroscopy) has been studied. This research aims to study the different markers of fat characters in meatballs containing the mixture of chicken fat, lard and rat fat. Sample preparation used Soxhlet extraction with n-hexane solvent and distillation of fat from the solvent. The extraction temperature is 70°C with solvent volume 200 mL. Fat samples were prepared by varying the ratio of 100% chicken fat concentration which corresponds to 1%, 10%, and 20% lard, and 1%, 10%, and 20% rat fat. The meatballs were made with a composition of 0%, 5% and 90% rat meat. The result of FTIR interpretation shows that the increasing concentration of the mixture of lard and rat fat have increased the absorption at wave numbers of 3371 cm-1, 3332 cm-1, 2337 cm-1, and 1743 cm-1. The FTIR spectrum is interpreted based on the uptake of typical functional groups of animal fats. The characteristics of animal fat properties can be distinguished by consistent results using infrared spectroscopy.

scholarly journals Bifidobacteria Strain Typing by Fourier Transform Infrared Spectroscopy

2021 ◽  
Vol 12 ◽  
Author(s):  
Francesca Deidda ◽  
Nicole Bozzi Cionci ◽  
Miriam Cordovana ◽  
Ilenia Campedelli ◽  
Fabio Fracchetti ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ftir Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Bifidobacterium Longum ◽  
Fourier Transform Infrared ◽  
The Other ◽  
Strain Typing ◽  
Covalent Bonds ◽  
Wide Range

Fourier transform infrared (FTIR) spectroscopy, a technology traditionally used in chemistry to determine the molecular composition of a wide range of sample types, has gained growing interest in microbial typing. It is based on the different vibrational modes of the covalent bonds between atoms of a given sample, as bacterial cells, induced by the absorption of infrared radiation. This technique has been largely used for the study of pathogenic species, especially in the clinical field, and has been proposed also for the typing at different subspecies levels. The high throughput, speed, low cost, and simplicity make FTIR spectroscopy an attractive technique also for industrial applications, in particular, for probiotics. The aim of this study was to compare FTIR spectroscopy with established genotyping methods, pulsed-field gel electrophoresis (PFGE), whole-genome sequencing (WGS), and multilocus sequence typing (MLST), in order to highlight the FTIR spectroscopy potential discriminatory power at strain level. Our study focused on bifidobacteria, an important group of intestinal commensals generally recognized as probiotics. For their properties in promoting and maintaining health, bifidobacteria are largely marketed by the pharmaceutical, food, and dairy industries. Strains belonging to Bifidobacterium longum subsp. longum and Bifidobacterium animalis subsp. lactis were taken into consideration together with some additional type strains. For B. longum subsp. longum, it was possible to discriminate the strains with all the methods used. Although two isolates were shown to be strictly phylogenetically related, constituting a unique cluster, based on PFGE, WGS, and MLST, no clustering was observed with FTIR. For B. animalis subsp. lactis group, PFGE, WGS, and MLST were non-discriminatory, and only one strain was easily distinguished. On the other hand, FTIR discriminated all the isolates one by one, and no clustering was observed. According to these results, FTIR analysis is not only equivalent to PFGE, WGS, and MLST, but also for some strains, in particular, for B. animalis subsp. lactis group, more informative, being able to differentiate strains not discernible with the other two methods based on phenotypic variations likely deriving from certain genetic changes. Fourier transform infrared spectroscopy has highlighted the possibility of using the cell surface as a kind of barcode making tracing strains possible, representing an important aspect in probiotic applications. Furthermore, this work constitutes the first investigation on bifidobacterial strain typing using FTIR spectroscopy.

scholarly journals Cell apoptosis specific marker found by Fourier Transform Infrared Spectroscopy

2004 ◽  
Vol 18 (3) ◽  
pp. 415-422 ◽  
Author(s):  
Silvia Gaudenzi ◽  
Deleana Pozzi ◽  
Paolo Toro ◽  
Ida Silvestri ◽  
Stefania Morrone ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ftir Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Ph Value ◽  
Fourier Transform Infrared ◽  
Specific Marker ◽  
Chemical Agent

We used Fourier Transform Infrared Spectroscopy (FTIR) combined with flow cytometry to study the apoptosis and necrosis processes in Jurkat, a lymphocyte cell line. The apoptosis was induced in the cells by a chemical agent, the actinomycin D, while the necrosis was induced lowering the pH value to 4.2. The apoptotic events were analysed by flow cytometry (using annexin V and propidium iodide) and contemporary monitored by FTIR spectroscopy at different times after the treatment. This comparison allowed us to find in the IR spectrum, between 3000 cm−1and 2800 cm−1, a “marker band” of the apoptosis corresponding to the exposure of phosphatidylserine on the outer leaflet of the membrane. A marker of a specific cellular process obtained by using a non‒destructive technique such as FTIR spectroscopy, has a great significance in the diagnostic medicine providing a tool for detecting pathologiesin vivo.

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