Cell apoptosis specific marker found by Fourier Transform Infrared Spectroscopy

We used Fourier Transform Infrared Spectroscopy (FTIR) combined with flow cytometry to study the apoptosis and necrosis processes in Jurkat, a lymphocyte cell line. The apoptosis was induced in the cells by a chemical agent, the actinomycin D, while the necrosis was induced lowering the pH value to 4.2. The apoptotic events were analysed by flow cytometry (using annexin V and propidium iodide) and contemporary monitored by FTIR spectroscopy at different times after the treatment. This comparison allowed us to find in the IR spectrum, between 3000 cm−1and 2800 cm−1, a “marker band” of the apoptosis corresponding to the exposure of phosphatidylserine on the outer leaflet of the membrane. A marker of a specific cellular process obtained by using a non‒destructive technique such as FTIR spectroscopy, has a great significance in the diagnostic medicine providing a tool for detecting pathologiesin vivo.

Download Full-text

In vivo study of the state of order of the membranes of Gram-negative bacteria by Fourier-transform infrared spectroscopy (FT-IR)

FEBS Letters ◽

10.1016/0014-5793(91)81339-a ◽

1991 ◽

Vol 294 (1-2) ◽

pp. 43-46 ◽

Cited By ~ 33

Author(s):

Christian Schultz ◽

Dieter Naumann

Keyword(s):

Fourier Transform ◽

Infrared Spectroscopy ◽

Fourier Transform Infrared Spectroscopy ◽

Fourier Transform Infrared ◽

The State ◽

In Vivo Study ◽

Gram Negative Bacteria ◽

Gram Negative ◽

Ft Ir

Download Full-text

Evaluation of in vivo adsorption of blood elements onto hydrogel-coated silicone rubber by scanning electron microscopy and fourier transform infrared spectroscopy

Journal of Biomedical Materials Research ◽

10.1002/jbm.820190916 ◽

1985 ◽

Vol 19 (9) ◽

pp. 1043-1071 ◽

Cited By ~ 29

Author(s):

Linda M. Seifert ◽

Raymond T. Greer

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Fourier Transform ◽

Infrared Spectroscopy ◽

Fourier Transform Infrared Spectroscopy ◽

Silicone Rubber ◽

Fourier Transform Infrared ◽

Blood Elements ◽

Scanning Electron

Download Full-text

A Green and Facile Approach for Synthesis of Starch-Pectin Magnetite Nanoparticles and Application by Removal of Methylene Blue from Textile Effluent

Journal of Nanomaterials ◽

10.1155/2019/4576135 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 2

Author(s):

Mih Venasius Nsom ◽

Ekane Peter Etape ◽

Josepha Foba Tendo ◽

Beckley Victorine Namond ◽

Paul T. Chongwain ◽

...

Keyword(s):

Methylene Blue ◽

Fourier Transform ◽

Infrared Spectroscopy ◽

Fourier Transform Infrared Spectroscopy ◽

Ph Value ◽

Polymer Concentration ◽

Fourier Transform Infrared ◽

Hybrid Nanoparticles ◽

Blue Dye ◽

Methylene Blue Dye

Pectin-starch magnetite hybrid nanoparticles were fabricated, characterized, and evaluated as potential absorbents for methylene blue dye based on recycling water from the textile industry. The nanocomposite adsorbent was synthesized with the iron salt coprecipitation method, and the precipitates obtained were sponge-like. The effects of a pectin : starch ratio in the absorbent and the amount of methylene blue adsorbed were investigated. The nanocomposites obtained were characterized using a Fourier-transform infrared spectroscopy (FTIR), before and after methylene blue adsorption. Fourier-transform infrared spectroscopy (FTIR) spectra provided the evidence that the starch-pectin iron oxide hybrid nanoparticles were successfully synthesized. It also indicated that the hybrid nanoparticles actually absorbed the methylene blue dye from the effluent. PXRD results showed that the synthesized hybrid composite adopted the spinel microstructure of Fe3O4 though the crystallinity of the composite decreased with an increase in the pectin : starch ratio. Furthermore, calculations based on PXRD showed that the synthesized powders were nanoparticles. The amount of adsorbed dye by hybrid adsorbent increased with an increase in the starch : pectin ratio, and the increase was better observed at a low polymer concentration of 18%. The amount of adsorbed dye by hybrid adsorbent was high at high pH and low at low pH value which attested to the ion exchange and electrostatic force mechanism during the adsorption process. Finally, the capacity of the absorbent decreased with an increase in temperature.

Download Full-text

Liposome-based formulations for the antibiotic nonapeptide Leucinostatin A: Fourier transform infrared spectroscopy characterization and in vivo toxicologic study

AAPS PharmSciTech ◽

10.1208/pt010102 ◽

2000 ◽

Vol 1 (1) ◽

pp. 9-19 ◽

Cited By ~ 1

Author(s):

Maurizio Ricci ◽

Paola Sassi ◽

Claudio Nastruzzi ◽

Carlo Róssi

Keyword(s):

Fourier Transform ◽

Infrared Spectroscopy ◽

Fourier Transform Infrared Spectroscopy ◽

Fourier Transform Infrared

Download Full-text

Investigation of interfacial structure of couping agent treated fillers by fourier transform infrared spectroscopy and attenuated total reflection-FTIR spectroscopy

Polymer Composites ◽

10.1002/pc.10409 ◽

2002 ◽

Vol 23 (1) ◽

pp. 28-33 ◽

Cited By ~ 10

Author(s):

Yujuan Liu ◽

Rui Yang ◽

Jian Yu ◽

Kunhua Wang

Keyword(s):

Fourier Transform ◽

Infrared Spectroscopy ◽

Ftir Spectroscopy ◽

Fourier Transform Infrared Spectroscopy ◽

Fourier Transform Infrared ◽

Total Reflection ◽

Attenuated Total Reflection ◽

Interfacial Structure

Download Full-text

Attenuated Total Reflection–Fourier Transform Infrared Spectroscopy as a Possible Method to Investigate Biophysical Parameters of Stratum Corneum In Vivo

Journal of Investigative Dermatology ◽

10.1046/j.1523-1747.2001.01262.x ◽

2001 ◽

Vol 116 (3) ◽

pp. 380-386 ◽

Cited By ~ 77

Author(s):

Lorenzo Brancaleon ◽

Michael Paul Bamberg ◽

Takeshi Sakamaki ◽

Nikiforos Kollias

Keyword(s):

Fourier Transform ◽

Infrared Spectroscopy ◽

Stratum Corneum ◽

Fourier Transform Infrared Spectroscopy ◽

Fourier Transform Infrared ◽

Total Reflection ◽

Attenuated Total Reflection ◽

Biophysical Parameters

Download Full-text

Time-resolved Fourier-transform infrared spectroscopy reveals the hidden bimolecular process of the ferrioxalate actinometer

Physical Chemistry Chemical Physics ◽

10.1039/c9cp05233j ◽

2019 ◽

Vol 21 (43) ◽

pp. 23803-23807 ◽

Cited By ~ 2

Author(s):

Frank Hendrik Pilz ◽

Jörg Lindner ◽

Peter Vöhringer

Keyword(s):

Fourier Transform ◽

Infrared Spectroscopy ◽

Ftir Spectroscopy ◽

Fourier Transform Infrared Spectroscopy ◽

Molecular Level ◽

Fourier Transform Infrared ◽

Bimolecular Reaction ◽

Time Resolved

Step-scan FTIR-spectroscopy reveals the bimolecular reaction in the ferrioxalate photochemistry, which builds the molecular-level foundation of the Hatchard–Parker actinometer.

Download Full-text

A Non-Toxic Depressant for Galena in Differential Flotation of Cu-Pb Sulfides

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.734-737.1018 ◽

2013 ◽

Vol 734-737 ◽

pp. 1018-1021 ◽

Cited By ~ 1

Author(s):

Xian Hui Qiu

Keyword(s):

Fourier Transform ◽

Infrared Spectroscopy ◽

Fourier Transform Infrared Spectroscopy ◽

Tannic Acid ◽

Ph Value ◽

Fourier Transform Infrared ◽

Lead Ions ◽

Hydroxyl Groups ◽

Natural Polymer ◽

Ph Range

Tannic acid, a natural polymer extracts from trees, was found to selectively depress galena while chalcopyrite was floated by xanthate. Micro-flotation studies, adsorption and Fourier transform infrared spectroscopy have been employed to investigate the mechanism. Micro-flotation studies shown that tannic acid can effectively depress galena while depress chalcopyrite weakly in the entire pH range. The adsorption results shown that the tannic acid adsorbed on chalcopyrite and galena surface influenced by the pH value, the higher the pH, the less of adsorption density. Tannic acid adsorb on galena through complexation with the lead ions, not with hydroxyl complex in the galena surface. Fourier transform infrared spectroscopy revealed that tannic acid interacted with lead ions by the hydroxyl groups on the phenol group. Tannic acid may be replace the hazardous inorganic depressants such as cyanide, dichromate and sulfur dioxide used in differential Cu-Pb sulfide separation.

Download Full-text